complete protease inhibitor mixture  (Roche)


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    Structured Review

    Roche complete protease inhibitor mixture
    Complete Protease Inhibitor Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mixture/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mixture - by Bioz Stars, 2021-05
    86/100 stars

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    Protease Inhibitor:

    Article Title: Kinetic Analysis of Arf GAP1 Indicates a Regulatory Role for Coatomer *
    Article Snippet: [1–415]Arf GAP1-His and [1–415]Arf GAP1-GFP were expressed in H5 insect cells with the BAC-to-BAC® baculovirus expression system following the manufacturer's protocol (Invitrogen). .. Pellets from 200 ml of H5 insect cells containing the expressed proteins were lysed in 20 m m Tris-HCl, pH 8.0, 150 m m NaCl, 10% glycerol, 0.1% Triton X-100 with a protease inhibitor mixture (Complete™, Roche Applied Science). ..

    Article Title: Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader
    Article Snippet: .. Briefly, HA-LPA1 -B103 or Vec-B103 cell pellets (∼6–7 × 106 cells) were resuspended in 1 ml of ice-cold PBS containing cOmplete™ protease inhibitor mixture (Roche) and transferred to a glass dram vial. .. Cell suspensions in an ice bath were then probe sonicated (Qsonica Q125 sonicator, 30–40% amplitude with an intense pulse sound; pulse: 5 s on, 1 s off, for 90 s) and the resulting solutions were centrifuged at 4°C for 1 h at 10,000 g .

    Article Title: Mapping sites of carboxymethyllysine modification on proteins reveals its consequences for proteostasis and cell proliferation
    Article Snippet: Fetal calf serum (FCS), human serum, endothelial growth supplement (ECGS), glyoxal (HUVEC studies), hydroxyurea, nocodazole, antimycin A, thymidine, 2-deoxy-D-glucose, trypsin inhibitor, thrombin, aprotinin, 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), 4’,6-diamidino-2-phenylindole (DAPI), octyl β-D-glucopyranoside, iodoacetamide (IAA), aqueous NH3 , ATP, cOmplete™, EDTA-free protease inhibitor cocktail, HEPES, MOPS, NP40 and PonceauS were purchased from Sigma (Taufkirchen, Germany). .. Protease inhibitor mixture complete, EDTA-free was obtained from Roche Diagnostics (Mannheim, Germany), fibrinogen from Merck/Millipore (Darmstadt, Germany) and Fluoromount-G® from Southern Biotech (Birmingham, AL, US), respectively. .. Bovine serum albumin-C (BSA-C) was from Aurion (Wageningen, The Netherlands) and goat serum from Cell Signaling Technology (Frankfurt, Germany).

    Article Title: FlashPack: Fast and Simple Preparation of Ultrahigh-performance Capillary Columns for LC-MS*
    Article Snippet: .. HeLa cell pellet or differentiating human myoblasts (−80 °C) were thawed at +4 °C in solubilization buffer containing 50 m m triethylammonium bicarbonate, 1% sodium deoxycholate, 10 m m tris(2-carboxyethyl)phosphine, 40 m m 2-chloroacetamide, pH 8.5, supplemented with a cOmplete™ Protease Inhibitor Mixture (Roche). ..

    Article Title: Nesfatin-1 Induces the Phosphorylation Levels of cAMP Response Element-Binding Protein for Intracellular Signaling in a Neural Cell Line
    Article Snippet: Western Blotting In order to detect phosphorylated cAMP response element-binding protein (CREB), i.e., pCREB, western blot analysis was performed using rabbit monoclonal antibodies: anti-CREB (CREB (48H2) #9197, Cell Signaling Technology, Danvers, USA) and anti-Phospho-CREB (Ser133) (#9191, Cell Signaling Technology). .. NB41A3 cells were lysed in RIPA buffer containing phosphate-buffered saline (PBS), 1% Igepal CA-630 (Sigma), 0.5% sodium deoxycholate, 0.1% SDS, 1 mM dithiothreitol, 1 mM sodium orthovanadate, 2% (v/w) Complete™ protease inhibitor mixture (Roche Molecular Biochemicals), and 0.1 mg/mL phenylmethylsufonyl fluoride. .. Next, 25 µg of the whole cell extract was subjected to SDS-PAGE, and the intensities of the bands corresponding to CREB and pCREB were quantitatively measured by using the ImageJ software (Rasband, W.S., ImageJ, US National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/ , 1997–2011).

    Article Title: Rescue by 4-phenylbutyrate of several misfolded creatine transporter-1 variants linked to the creatine transporter deficiency syndrome.
    Article Snippet: .. Other cell culture reagents, such as BSA and Complete™ protease inhibitor mixture, were purchased from Roche Applied Science, SDS from BioMol (Hamburg, Germany), scintillation mixture (Rotiszint® eco plus), and Tris from Carl Roth (Karlsruhe, Germany). .. The rabbit polyclonal anti-GFP antibody (ab290) was obtained from Abcam (Cambridge, UK).

    Article Title: Convergence of Multiple Autophagy and Cytoplasm to Vacuole Targeting Components to a Perivacuolar Membrane Compartment Prior to de Novo Vesicle Formation *
    Article Snippet: .. Complete™ EDTA-free protease inhibitor mixture was from Roche Molecular Biochemicals. ..

    Article Title: P2X7 Receptor is Involved in Mitochondrial Dysfunction Induced by Extracellular Alpha Synuclein in Neuroblastoma SH-SY5Y Cells
    Article Snippet: Clarity™ Western ECL Substrate was purchased from Bio-Rad Laboratories (Hercules, CA, USA). .. Complete® protease inhibitor mixture tablets were purchased from Roche Diagnostics. .. Cell lysis buffer and antibodies, such as rabbit antiparkin (#2132), rabbit anti-AMPK (#5832), rabbit anti-Ulk-1 (#8054), rabbit anti-p-Ulk-1 (#5869), rabbit anti-p-AMPK (#2535), rabbit anti-LC3-II (#2775), were obtained from Cell Signaling Technology (Beverly, MA, USA).

    Cell Culture:

    Article Title: Rescue by 4-phenylbutyrate of several misfolded creatine transporter-1 variants linked to the creatine transporter deficiency syndrome.
    Article Snippet: .. Other cell culture reagents, such as BSA and Complete™ protease inhibitor mixture, were purchased from Roche Applied Science, SDS from BioMol (Hamburg, Germany), scintillation mixture (Rotiszint® eco plus), and Tris from Carl Roth (Karlsruhe, Germany). .. The rabbit polyclonal anti-GFP antibody (ab290) was obtained from Abcam (Cambridge, UK).

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    Roche complete protease inhibitor mix
    Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel <t>complete</t> ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.
    Complete Protease Inhibitor Mix, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mix/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mix - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Roche complete protease inhibitor mixture
    The ShK domains from L. sigmodontis protein nLs_04059 and its orthologs in other filarial species have a distinct sequence signature. All ShK domains identified in the <t>complete</t> theoretical proteomes of L. sigmodontis, B. malayi, L. loa, W. bancrofti,
    Complete Protease Inhibitor Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mixture/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mixture - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Roche caspase lysis buffer
    Nutlin prevents <t>caspase</t> activation and γH2AX accumulation in response to Wee1 inhibitor and/or gemcitabine A . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, and/or 8μM Nutlin in the absence and presence of 50μM ZVAD-FMK for another 24 hrs. Cells were harvested and immunoblot analysis was performed to detect poly-ADP ribose polymerase (PARP) and γH2AX. B ., C . U2OS cells were treated as in (A). The cells were then fixed and stained for γH2AX by immunofluorescence. Detection and analysis was performed using automated immunofluorescence microscopy (BD Pathway). Figure panel (B) shows images of γH2AX staining for each treatment condition. Quantitation of γH2AX intensities was done using the BD pathway analysis tool and depicted in figure panel (C). Error bars represent the SD, n=3. D . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, 8μM Nutlin in the absence and presence ( Supplementary Figure 1 ) of 50μM ZVAD-FMK for another 24 hrs. The cells were harvested and lysed for caspase activity assay. Fluorescent intensity measurements were obtained for each treatment. The activity (arbitrary units of fluorescence/min) was calculated for each treatment at the linear part of the curve (cf. Supplementary Figure 1 ). Error bars represent the S.D, n=3.
    Caspase Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase lysis buffer/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caspase lysis buffer - by Bioz Stars, 2021-05
    86/100 stars
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    86
    Roche applied science complete protease inhibitor mixture tablets
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Applied Science Complete Protease Inhibitor Mixture Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied science complete protease inhibitor mixture tablets/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    applied science complete protease inhibitor mixture tablets - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

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    Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel complete ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.

    Journal: PLoS ONE

    Article Title: Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown

    doi: 10.1371/journal.pone.0013568

    Figure Lengend Snippet: Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel complete ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.

    Article Snippet: Western Blotting For generation of whole cell protein extracts, cells grown to subconfluency in 25 cm2 flasks were stimulated as indicated, washed with icecold PBS and scraped into radioimmunoprecipitation assay (RIPA) buffer composed of 50 mmol/L Tris-HCl, pH 7.4; 150 mmol/L NaCl; 1% Nonidet P40; 0.25% sodium deoxycholate and 1× Roche COMPLETE® protease inhibitor mix (Roche Diagnostics GmbH, Mannheim, Germany).

    Techniques: Mass Spectrometry, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    The ShK domains from L. sigmodontis protein nLs_04059 and its orthologs in other filarial species have a distinct sequence signature. All ShK domains identified in the complete theoretical proteomes of L. sigmodontis, B. malayi, L. loa, W. bancrofti,

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Comparative Analysis of the Secretome from a Model Filarial Nematode (Litomosoides sigmodontis) Reveals Maximal Diversity in Gravid Female Parasites *

    doi: 10.1074/mcp.M114.038539

    Figure Lengend Snippet: The ShK domains from L. sigmodontis protein nLs_04059 and its orthologs in other filarial species have a distinct sequence signature. All ShK domains identified in the complete theoretical proteomes of L. sigmodontis, B. malayi, L. loa, W. bancrofti,

    Article Snippet: Soluble WBE was prepared by homogenization in 25 m m ammonium bicarbonate, 1% Rapi Gest SF surfactant (Waters), and cOmplete Protease Inhibitor Mixture (Roche) using a mini-pestle in a microcentrifuge tube.

    Techniques: Sequencing

    Pfam enrichment analysis of ESP proteins against the complete theoretical proteome of L. sigmodontis . The fold-enrichment is displayed for each lifecycle stage; DUF290 represents the transthyretin-like protein family.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Comparative Analysis of the Secretome from a Model Filarial Nematode (Litomosoides sigmodontis) Reveals Maximal Diversity in Gravid Female Parasites *

    doi: 10.1074/mcp.M114.038539

    Figure Lengend Snippet: Pfam enrichment analysis of ESP proteins against the complete theoretical proteome of L. sigmodontis . The fold-enrichment is displayed for each lifecycle stage; DUF290 represents the transthyretin-like protein family.

    Article Snippet: Soluble WBE was prepared by homogenization in 25 m m ammonium bicarbonate, 1% Rapi Gest SF surfactant (Waters), and cOmplete Protease Inhibitor Mixture (Roche) using a mini-pestle in a microcentrifuge tube.

    Techniques: End-sequence Profiling

    Nutlin prevents caspase activation and γH2AX accumulation in response to Wee1 inhibitor and/or gemcitabine A . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, and/or 8μM Nutlin in the absence and presence of 50μM ZVAD-FMK for another 24 hrs. Cells were harvested and immunoblot analysis was performed to detect poly-ADP ribose polymerase (PARP) and γH2AX. B ., C . U2OS cells were treated as in (A). The cells were then fixed and stained for γH2AX by immunofluorescence. Detection and analysis was performed using automated immunofluorescence microscopy (BD Pathway). Figure panel (B) shows images of γH2AX staining for each treatment condition. Quantitation of γH2AX intensities was done using the BD pathway analysis tool and depicted in figure panel (C). Error bars represent the SD, n=3. D . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, 8μM Nutlin in the absence and presence ( Supplementary Figure 1 ) of 50μM ZVAD-FMK for another 24 hrs. The cells were harvested and lysed for caspase activity assay. Fluorescent intensity measurements were obtained for each treatment. The activity (arbitrary units of fluorescence/min) was calculated for each treatment at the linear part of the curve (cf. Supplementary Figure 1 ). Error bars represent the S.D, n=3.

    Journal: Oncotarget

    Article Title: Mdm2 inhibition confers protection of p53-proficient cells from the cytotoxic effects of Wee1 inhibitors

    doi:

    Figure Lengend Snippet: Nutlin prevents caspase activation and γH2AX accumulation in response to Wee1 inhibitor and/or gemcitabine A . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, and/or 8μM Nutlin in the absence and presence of 50μM ZVAD-FMK for another 24 hrs. Cells were harvested and immunoblot analysis was performed to detect poly-ADP ribose polymerase (PARP) and γH2AX. B ., C . U2OS cells were treated as in (A). The cells were then fixed and stained for γH2AX by immunofluorescence. Detection and analysis was performed using automated immunofluorescence microscopy (BD Pathway). Figure panel (B) shows images of γH2AX staining for each treatment condition. Quantitation of γH2AX intensities was done using the BD pathway analysis tool and depicted in figure panel (C). Error bars represent the SD, n=3. D . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, 8μM Nutlin in the absence and presence ( Supplementary Figure 1 ) of 50μM ZVAD-FMK for another 24 hrs. The cells were harvested and lysed for caspase activity assay. Fluorescent intensity measurements were obtained for each treatment. The activity (arbitrary units of fluorescence/min) was calculated for each treatment at the linear part of the curve (cf. Supplementary Figure 1 ). Error bars represent the S.D, n=3.

    Article Snippet: The pelleted cells were resuspended in 250μl caspase lysis buffer (1M Tris-HCl, 2mM MgCl2 , 150mM NaCl, 10mM DTT, Roche complete mini protease-inhibitor mix).

    Techniques: Activation Assay, Staining, Immunofluorescence, Microscopy, Quantitation Assay, Caspase Activity Assay, Activity Assay, Fluorescence

    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Journal: The Journal of Biological Chemistry

    Article Title: Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site

    doi: 10.1074/jbc.M117.775981

    Figure Lengend Snippet: The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Article Snippet: Cells were resuspended in 50 m m NaH2 PO4 , 300 m m NaCl, pH 8.0, with Roche Applied Science cOmplete protease inhibitor mixture tablets.

    Techniques: Activation Assay, Activity Assay