complete protease inhibitor mix  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Roche complete protease inhibitor mix
    Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel <t>complete</t> ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.
    Complete Protease Inhibitor Mix, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mix/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mix - by Bioz Stars, 2021-06
    86/100 stars

    Images

    1) Product Images from "Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown"

    Article Title: Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0013568

    Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel complete ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.
    Figure Legend Snippet: Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel complete ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.

    Techniques Used: Mass Spectrometry, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Related Articles

    Western Blot:

    Article Title: Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell
    Article Snippet: Peptides of in-gel trypsin-digested protein bands were separated by liquid chromatography on a reversed-phase C18 ). .. For Western immunoblotting, cell extracts from PC12 cells, COS7 cells and chromaffin cells were prepared in buffer A containing 1% Triton X-100 and the Complete™ protease inhibitors mix (Roche, Welwyn Garden City, U.K.). .. Detergent extracts from other cell lines, organs and brain parts were from GenoTech (St Louis, MO, U.S.A.).

    Article Title: From a Movement-Deficient Grapevine Fanleaf Virus to the Identification of a New Viral Determinant of Nematode Transmission
    Article Snippet: Film-based photographs were acquired onto Kodak Electron Image Films SO-163 and developed. .. Western Blot Analysis For each sample, three discs 1 cm in diameter were collected from agro-infiltrated N. benthamiana leaf patches, five days post infiltration (dpi), ground in 270 µL of phosphate buffered saline (pH 7.4) in the presence of protease inhibitors (cOmplete™: Roche Diagnostics GmbH, Mannheim, Germany), and mixed with 1 volume (w/v) of Laemmli buffer 2X [ ]. .. Total soluble proteins were heat-denatured 10 min at 90 °C and clarified at 10,000 g for 5 min prior to separation on an 8% acrylamide SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).

    Protease Inhibitor:

    Article Title: Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro
    Article Snippet: The translation reaction was set up using modified standard conditions ( ). .. The reaction (10 μl) contained 50% tRNA-depleted LTE (5 μl), 1.67 μl of 3 μg/μl of antisense-oligonucleotide treated total tRNA, 4.45 mM Mg(OAc)2 , 0.24 mM spermidine, 2 mM DTT, 40 mM creatine phosphate, 20 mM HEPES–KOH pH 7.6, 1% (v/v) PEG 3000, 1× protease inhibitor (Complete™ EDTA-free, Roche), 0.14 mM of each amino acid, 1.5 mM rNTP mix (ATP, GTP, UTP and CTP), 0.01 mM anti-splice leader DNA oligonucleotide, 0.1 mg/ml T7 RNA polymerase, 40 U/ml creatine phosphokinase, 50–100 ng/μl DNA template, with or without 20 μM AGC-suppressor tRNA (L. tarentolae tRNAGly GCU) as indicated. .. For inactivation of selected tRNAs directly in LTE, the cell extract was incubated with antisense oligonucleotides at 30 μM concentration at 37°C for 5 min and then cooled down on ice prior to assembly of the in vitro translation reaction.

    Article Title: Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro
    Article Snippet: The translation reaction was set up using modified standard conditions ( ). .. The reaction (10 μl) contained 50% tRNA-depleted LTE (5 μl), 1.67 μl of 3 μg/μl of antisense-oligonucleotide treated total tRNA, 4.45 mM Mg(OAc)2 , 0.24 mM spermidine, 2 mM DTT, 40 mM creatine phosphate, 20 mM HEPES–KOH pH 7.6, 1% (v/v) PEG 3000, 1× protease inhibitor (Complete™ EDTA-free, Roche), 0.14 mM of each amino acid, 1.5 mM rNTP mix (ATP, GTP, UTP and CTP), 0.01 mM anti-splice leader DNA oligonucleotide, 0.1 mg/ml T7 RNA polymerase, 40 U/ml creatine phosphokinase, 50–100 ng/μl DNA template, with or without 20 μM AGC-suppressor tRNA ( L. tarentolae tRNAGly GCU) as indicated. .. For inactivation of selected tRNAs directly in LTE, the cell extract was incubated with antisense oligonucleotides at 30 μM concentration at 37°C for 5 min and then cooled down on ice prior to assembly of the in vitro translation reaction.

    Article Title: Exosomes induce endolysosomal permeabilization as a gateway by which exosomal tau seeds escape into the cytosol
    Article Snippet: This preparation of exosome pellets was resuspended in 2 ml of 0.95-M sucrose in 20-mM HEPES (15630-080, Life Technologies), after which a sucrose step gradient (six 2-ml steps: 2.0, 1.65, 1.3, 0.95, 0.6, and 0.25 M on top) was used to purify the exosomes by centrifugation at 200,000g for 16 h at 4 °C. .. Finally, the sucrose-purified exosomes floating in the interphase at 0.95-M sucrose were recovered, washed with 5-ml PBS, ultracentrifuged again, and the exosome pellet resuspended in 120-µl PBS containing 1 × Complete protease inhibitor cocktail (Roche). .. Protein content was quantified with a BCA™ Protein Assay Kit (23227, Thermo-Fisher) using a 15-µl aliquot of exosomes in PBS, which was mixed with 15 µl of 1 × RIPA buffer (150-mM NaCl, 50-mM Tris–HCl pH7.4, 0.5% (w/v) sodium deoxycholate, 1.0% (v/v) Nonidet P-40, 0.1% (w/v) SDS, 5-mM EDTA, 50-mM NaF) supplemented with protease inhibitors, and then homogenized in a water bath sonicator for 10 min.

    Article Title: Rapamycin conditionally inhibits Hsp90 but not Hsp70 mRNA translation in Drosophila: implications for the mechanisms of Hsp mRNA translation
    Article Snippet: .. The cell pellets were resuspended in 1000 μl of 4°C m7 GTP column buffer (100 mM KCl, 20 mM HEPES pH 7.6, 7 mM β-mercaptoethanol, 0.2 mM EDTA, 10% glycerol) to which the following components were added for lysis: Triton ×-100 to 0.1% and Complete ™ protease inhibitor mix (Roche). ..

    Article Title: Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy
    Article Snippet: Seedlings of 5 days old pldα1-1 PLDα1-YFP and pldα1-2 PLDα1-YFP complemented plants, a progeny from one selected T2 plant from each independent transgenic SALK line, were used for immunoblotting analysis. .. Roots from ~50 seedlings of 14 days old plants of A. thaliana , ecotype Col-0, pldα1-1 , and pldα1-2 single mutants as well as pldα1-1 PLDα1-YFP and pldα1-2 PLDα1-YFP complemented lines were homogenized using liquid nitrogen to fine powder and the proteins were extracted in E-buffer [50 mM HEPES (pH 7.5), 75 mM NaCl, 1 mM EGTA, 1 mM MgCl2 , 1 mM NaF, 10% (v/v) glycerol, Complete™ EDTA-free protease inhibitor and PhosSTOP™ phosphatase inhibitor cocktails (both from Roche, Basel, Switzerland]. .. After centrifugation at 13000 g in 4°C for 15 min, supernatants were mixed with 4-fold concentrated Laemmli buffer [final concentration 62.5 mM Tris-HCl (pH 6.8), 2% (w/v) SDS, 10% (v/v) glycerol, 300 mM 2-mercaptoethanol] and boiled for 5 min.

    Lysis:

    Article Title: Rapamycin conditionally inhibits Hsp90 but not Hsp70 mRNA translation in Drosophila: implications for the mechanisms of Hsp mRNA translation
    Article Snippet: .. The cell pellets were resuspended in 1000 μl of 4°C m7 GTP column buffer (100 mM KCl, 20 mM HEPES pH 7.6, 7 mM β-mercaptoethanol, 0.2 mM EDTA, 10% glycerol) to which the following components were added for lysis: Triton ×-100 to 0.1% and Complete ™ protease inhibitor mix (Roche). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Roche complete protease inhibitor mix
    Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel <t>complete</t> ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.
    Complete Protease Inhibitor Mix, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mix/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mix - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche complete protease inhibitor mixture
    The ShK domains from L. sigmodontis protein nLs_04059 and its orthologs in other filarial species have a distinct sequence signature. All ShK domains identified in the <t>complete</t> theoretical proteomes of L. sigmodontis, B. malayi, L. loa, W. bancrofti,
    Complete Protease Inhibitor Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mixture/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mixture - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche caspase lysis buffer
    Nutlin prevents <t>caspase</t> activation and γH2AX accumulation in response to Wee1 inhibitor and/or gemcitabine A . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, and/or 8μM Nutlin in the absence and presence of 50μM ZVAD-FMK for another 24 hrs. Cells were harvested and immunoblot analysis was performed to detect poly-ADP ribose polymerase (PARP) and γH2AX. B ., C . U2OS cells were treated as in (A). The cells were then fixed and stained for γH2AX by immunofluorescence. Detection and analysis was performed using automated immunofluorescence microscopy (BD Pathway). Figure panel (B) shows images of γH2AX staining for each treatment condition. Quantitation of γH2AX intensities was done using the BD pathway analysis tool and depicted in figure panel (C). Error bars represent the SD, n=3. D . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, 8μM Nutlin in the absence and presence ( Supplementary Figure 1 ) of 50μM ZVAD-FMK for another 24 hrs. The cells were harvested and lysed for caspase activity assay. Fluorescent intensity measurements were obtained for each treatment. The activity (arbitrary units of fluorescence/min) was calculated for each treatment at the linear part of the curve (cf. Supplementary Figure 1 ). Error bars represent the S.D, n=3.
    Caspase Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase lysis buffer/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caspase lysis buffer - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche applied science complete protease inhibitor mixture tablets
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Applied Science Complete Protease Inhibitor Mixture Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied science complete protease inhibitor mixture tablets/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    applied science complete protease inhibitor mixture tablets - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel complete ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.

    Journal: PLoS ONE

    Article Title: Evaluation of Soluble Junctional Adhesion Molecule-A as a Biomarker of Human Brain Endothelial Barrier Breakdown

    doi: 10.1371/journal.pone.0013568

    Figure Lengend Snippet: Soluble JAM-A serum levels do not indicate BBB disturbance in multiple sclerosis and ischemic stroke. A Levels of sJAM-A in serum samples from 45 patients with stable relapsing-remitting MS not receiving secondary prophylactic therapy were measured in duplicates by ELISA and compared to those in 14 untreated MS patients during an acute relapse. B Levels of sJAM-A were measured in serum samples from 13 patients with non-small vessel complete ischemic stroke where a first sample was obtained within the first 3 h of symptom onset and a second sample 24 h after clinical onset. Serum samples were not concentrated before ELISA measurement. Dots represent single values, lines median values. Serum levels in stable and inflammatory-active MS patients were statistically compared by the Mann-Whitney U test, serum levels in stroke patients over time by the Wilcoxon matched pairs test. n.s., not significant.

    Article Snippet: Western Blotting For generation of whole cell protein extracts, cells grown to subconfluency in 25 cm2 flasks were stimulated as indicated, washed with icecold PBS and scraped into radioimmunoprecipitation assay (RIPA) buffer composed of 50 mmol/L Tris-HCl, pH 7.4; 150 mmol/L NaCl; 1% Nonidet P40; 0.25% sodium deoxycholate and 1× Roche COMPLETE® protease inhibitor mix (Roche Diagnostics GmbH, Mannheim, Germany).

    Techniques: Mass Spectrometry, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    The ShK domains from L. sigmodontis protein nLs_04059 and its orthologs in other filarial species have a distinct sequence signature. All ShK domains identified in the complete theoretical proteomes of L. sigmodontis, B. malayi, L. loa, W. bancrofti,

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Comparative Analysis of the Secretome from a Model Filarial Nematode (Litomosoides sigmodontis) Reveals Maximal Diversity in Gravid Female Parasites *

    doi: 10.1074/mcp.M114.038539

    Figure Lengend Snippet: The ShK domains from L. sigmodontis protein nLs_04059 and its orthologs in other filarial species have a distinct sequence signature. All ShK domains identified in the complete theoretical proteomes of L. sigmodontis, B. malayi, L. loa, W. bancrofti,

    Article Snippet: Soluble WBE was prepared by homogenization in 25 m m ammonium bicarbonate, 1% Rapi Gest SF surfactant (Waters), and cOmplete Protease Inhibitor Mixture (Roche) using a mini-pestle in a microcentrifuge tube.

    Techniques: Sequencing

    Pfam enrichment analysis of ESP proteins against the complete theoretical proteome of L. sigmodontis . The fold-enrichment is displayed for each lifecycle stage; DUF290 represents the transthyretin-like protein family.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Comparative Analysis of the Secretome from a Model Filarial Nematode (Litomosoides sigmodontis) Reveals Maximal Diversity in Gravid Female Parasites *

    doi: 10.1074/mcp.M114.038539

    Figure Lengend Snippet: Pfam enrichment analysis of ESP proteins against the complete theoretical proteome of L. sigmodontis . The fold-enrichment is displayed for each lifecycle stage; DUF290 represents the transthyretin-like protein family.

    Article Snippet: Soluble WBE was prepared by homogenization in 25 m m ammonium bicarbonate, 1% Rapi Gest SF surfactant (Waters), and cOmplete Protease Inhibitor Mixture (Roche) using a mini-pestle in a microcentrifuge tube.

    Techniques: End-sequence Profiling

    Nutlin prevents caspase activation and γH2AX accumulation in response to Wee1 inhibitor and/or gemcitabine A . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, and/or 8μM Nutlin in the absence and presence of 50μM ZVAD-FMK for another 24 hrs. Cells were harvested and immunoblot analysis was performed to detect poly-ADP ribose polymerase (PARP) and γH2AX. B ., C . U2OS cells were treated as in (A). The cells were then fixed and stained for γH2AX by immunofluorescence. Detection and analysis was performed using automated immunofluorescence microscopy (BD Pathway). Figure panel (B) shows images of γH2AX staining for each treatment condition. Quantitation of γH2AX intensities was done using the BD pathway analysis tool and depicted in figure panel (C). Error bars represent the SD, n=3. D . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, 8μM Nutlin in the absence and presence ( Supplementary Figure 1 ) of 50μM ZVAD-FMK for another 24 hrs. The cells were harvested and lysed for caspase activity assay. Fluorescent intensity measurements were obtained for each treatment. The activity (arbitrary units of fluorescence/min) was calculated for each treatment at the linear part of the curve (cf. Supplementary Figure 1 ). Error bars represent the S.D, n=3.

    Journal: Oncotarget

    Article Title: Mdm2 inhibition confers protection of p53-proficient cells from the cytotoxic effects of Wee1 inhibitors

    doi:

    Figure Lengend Snippet: Nutlin prevents caspase activation and γH2AX accumulation in response to Wee1 inhibitor and/or gemcitabine A . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, and/or 8μM Nutlin in the absence and presence of 50μM ZVAD-FMK for another 24 hrs. Cells were harvested and immunoblot analysis was performed to detect poly-ADP ribose polymerase (PARP) and γH2AX. B ., C . U2OS cells were treated as in (A). The cells were then fixed and stained for γH2AX by immunofluorescence. Detection and analysis was performed using automated immunofluorescence microscopy (BD Pathway). Figure panel (B) shows images of γH2AX staining for each treatment condition. Quantitation of γH2AX intensities was done using the BD pathway analysis tool and depicted in figure panel (C). Error bars represent the SD, n=3. D . U2OS cells were treated with 8μM Nutlin for 24 hrs, followed by treatment with 1μM Wee1 inhibitor, 300nM gemcitabine, 8μM Nutlin in the absence and presence ( Supplementary Figure 1 ) of 50μM ZVAD-FMK for another 24 hrs. The cells were harvested and lysed for caspase activity assay. Fluorescent intensity measurements were obtained for each treatment. The activity (arbitrary units of fluorescence/min) was calculated for each treatment at the linear part of the curve (cf. Supplementary Figure 1 ). Error bars represent the S.D, n=3.

    Article Snippet: The pelleted cells were resuspended in 250μl caspase lysis buffer (1M Tris-HCl, 2mM MgCl2 , 150mM NaCl, 10mM DTT, Roche complete mini protease-inhibitor mix).

    Techniques: Activation Assay, Staining, Immunofluorescence, Microscopy, Quantitation Assay, Caspase Activity Assay, Activity Assay, Fluorescence

    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Journal: The Journal of Biological Chemistry

    Article Title: Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site

    doi: 10.1074/jbc.M117.775981

    Figure Lengend Snippet: The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Article Snippet: Cells were resuspended in 50 m m NaH2 PO4 , 300 m m NaCl, pH 8.0, with Roche Applied Science cOmplete protease inhibitor mixture tablets.

    Techniques: Activation Assay, Activity Assay