complete hank s balanced salt solution  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher complete hank s balanced salt solution
    Complete Hank S Balanced Salt Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete hank s balanced salt solution/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete hank s balanced salt solution - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Stable Transfection:

    Article Title: Functional drug screening reveals anticonvulsants as enhancers of mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion
    Article Snippet: .. Mitochondrial Ca2+ measurements HeLa cells stably expressing mt-cameleon were seeded onto uncoated 35-mm glass-bottomed dishes (MatTek Corporation), grown to 40–70% confluence then treated with 50 μM carbamazepine or vehicle alone for 24 h. Shortly, before imaging, cells were rinsed twice and maintained in calcium containing Hanks' balanced salt solution (HBSS, Gibco) supplemented with 1 mM HEPES (Sigma) and 20% glucose at 37 °C. .. Fluorescence imaging was achieved as previously described (Tolhurst et al , ), based on Palmer et al ( ).

    Blocking Assay:

    Article Title: The Effect of the Nonionic Block Copolymer Pluronic P85 on Gene Expression in Mouse Muscle and Antigen Presenting Cells
    Article Snippet: .. The plasmid DNA and block copolymer stock solutions were added to Gibco™ Hanks Balanced Salt Solution (HBSS) buffer (Invitrogen, Carlsbad, CA) and gently mixed to obtain required concentrations (in case of SP1017 the total block copolymer concentrations at various dilutions are presented). ..

    Incubation:

    Article Title: Transdifferentiation of periodontal ligament-derived stem cells into retinal ganglion-like cells and its microRNA signature
    Article Snippet: .. Briefly, the treated or control PDLSCs at Day 24 were incubated in Hanks’ balanced salt solution (HBSS, Ca2+ /Mg2+ -free; Gibco BRL) containing 5μM Fluo-4AM and 0.1% pluronic F-127 (Sigma-Aldrich) for 30 minutes at room temperature. .. After washing with HBSS, 1 μM glutamate (Sigma-Aldrich) was added to the culture, and fluorescence images were immediately captured using a fluorescence microscope (Eclipse Ni-C; Nikon, Japan), with excitation wavelength at 495 nm and emission wavelength at 515 nm, every 5 seconds for a total of 2 minutes under a 40X objective lens.

    other:

    Article Title: Teaching an Old Drug New Tricks: Agonism, Antagonism, and Biased Signaling of Pilocarpine through M3 Muscarinic Acetylcholine Receptor
    Article Snippet: Hanks’ balanced salt solution (HBSS) with or without Ca2+ and fura-2, AM were acquired from Life Technologies (Carlsbad, CA).

    Imaging:

    Article Title: Functional drug screening reveals anticonvulsants as enhancers of mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion
    Article Snippet: .. Mitochondrial Ca2+ measurements HeLa cells stably expressing mt-cameleon were seeded onto uncoated 35-mm glass-bottomed dishes (MatTek Corporation), grown to 40–70% confluence then treated with 50 μM carbamazepine or vehicle alone for 24 h. Shortly, before imaging, cells were rinsed twice and maintained in calcium containing Hanks' balanced salt solution (HBSS, Gibco) supplemented with 1 mM HEPES (Sigma) and 20% glucose at 37 °C. .. Fluorescence imaging was achieved as previously described (Tolhurst et al , ), based on Palmer et al ( ).

    Expressing:

    Article Title: Functional drug screening reveals anticonvulsants as enhancers of mTOR-independent autophagic killing of Mycobacterium tuberculosis through inositol depletion
    Article Snippet: .. Mitochondrial Ca2+ measurements HeLa cells stably expressing mt-cameleon were seeded onto uncoated 35-mm glass-bottomed dishes (MatTek Corporation), grown to 40–70% confluence then treated with 50 μM carbamazepine or vehicle alone for 24 h. Shortly, before imaging, cells were rinsed twice and maintained in calcium containing Hanks' balanced salt solution (HBSS, Gibco) supplemented with 1 mM HEPES (Sigma) and 20% glucose at 37 °C. .. Fluorescence imaging was achieved as previously described (Tolhurst et al , ), based on Palmer et al ( ).

    Plasmid Preparation:

    Article Title: The Effect of the Nonionic Block Copolymer Pluronic P85 on Gene Expression in Mouse Muscle and Antigen Presenting Cells
    Article Snippet: .. The plasmid DNA and block copolymer stock solutions were added to Gibco™ Hanks Balanced Salt Solution (HBSS) buffer (Invitrogen, Carlsbad, CA) and gently mixed to obtain required concentrations (in case of SP1017 the total block copolymer concentrations at various dilutions are presented). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher hank s balanced salt solution hbss
    Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor <t>[Hank’s</t> balanced salt solution <t>(HBSS)].</t> The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p
    Hank S Balanced Salt Solution Hbss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hank s balanced salt solution hbss/product/Thermo Fisher
    Average 93 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    hank s balanced salt solution hbss - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor [Hank’s balanced salt solution (HBSS)]. The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p

    Journal: Frontiers in Veterinary Science

    Article Title: Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation

    doi: 10.3389/fvets.2017.00159

    Figure Lengend Snippet: Misoprostol pretreatment inhibits CXCL8-, LTB 4 -, and PAF-induced equine neutrophil chemotaxis. Calcein AM-labeled equine neutrophils were pretreated with multiple concentrations of misoprostol, dibutyryl cyclic-AMP (db-cAMP), or the vehicle control (VC) for each inhibitor [Hank’s balanced salt solution (HBSS)]. The phosphatidylinositol-3 kinase (PI3K)-inhibitor wortmannin was used as a positive control for inhibition. Neutrophils were stimulated for 1 h with the following chemoattractants or vehicles: (A) 100 ng/ml CXCL8 (or HBSS vehicle), (B) 10 nM LTB 4 (or EtOH vehicle), or (C) 10 nM PAF (or EtOH vehicle). Percent chemotaxis was calculated by dividing florescence in each bottom well by 100% migration control wells. Data are expressed as mean% chemotaxis ± SEM. ** p

    Article Snippet: Experimental Reagents Lipopolysaccharide (LPS) from E. coli 055:B5, phorbol 12-myristate 13-acetate (PMA), CXCL8, dibutyryl cyclic AMP (db-cAMP), wortmannin, staurosporine, bovine serum albumin (BSA), and anti-BSA antibody were from Sigma Aldrich (St. Louis, MO, USA); heat-inactivated fetal bovine serum (FBS) was from Gemini-Bioproducts (West Sacramento, CA, USA); misoprostol, LTB4 , and PAF were from Cayman Chemical (Ann Arbor, MI, USA); equine recombinant granulocyte-monocyte colony-stimulating factor (GM-CSF) was from Kingfisher Biotech (Saint Paul, MN, USA); and Hank’s balanced salt solution (HBSS) was from Thermo Fisher Scientific (Grand Island, NY, USA).

    Techniques: Chemotaxis Assay, Labeling, Positive Control, Inhibition, Migration