Complementary Dna Cdna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complementary dna cdna/product/Illumina Inc
Average 99 stars, based on 13 article reviews
Price from $9.99 to $1999.99
Related Products / Commonly Used Together
1) Product Images from "Circulating CD1c+ myeloid dendritic cells are potential precursors to LCH lesion CD1a+CD207+ cells"
Article Title: Circulating CD1c+ myeloid dendritic cells are potential precursors to LCH lesion CD1a+CD207+ cells
Journal: Blood Advances
Figure Legend Snippet: HLA-DQB2 expression is identified on blood BRAF-V600E + CD1c + mDCs in patients with high-risk (HR) LCH. (A) Genomic DNA was isolated from peripheral blood specimens from LCH patients (N = 11; n = 3 for high-risk multisystem, n = 3 for low-risk [LR] multisystem, n = 5 for low-risk single lesion) with BRAF V600E + lesions and healthy donors (N = 11). The percentage of circulating cells with BRAF V600 E As expected from previous studies, BRAF V600E expression was detected at low levels in PBMCs from patients with BRAF V600E + Technical duplicates were used in this experiment. LCH patients’ PBMC samples used in the study are listed in supplemental Table 1b. Blue dot, Patient LCH 0019; red dot, patient LCH 0020; green dot, patient LCH 0021. (B) RNA from peripheral blood specimens from the same set of LCH patients as in panel A was extracted and cDNA was amplified, and then the HLA-DQB2 expression was determined by qPCR (normalized to GAPDH mRNA expression). HLA-DQB2 expression was specifically detected in PBMCs from the same patients with detectable BRAF V600E + PBMCs. Technical duplicates were used in this experiment. Blue dot, Patient LCH 0019; red dot, patient LCH 0020; green dot, patient LCH 0021). (C) Representative dot plots showing identification of CD1c + mDCs (green gate) within HLA-DR + cells from PBMCs of an HR LCH patient. Overlay histograms show HLA-DQB2 expression in LCH lesion CD1c + mDCs (green) compared with control (gray). HLA-DQB2 expression was detectable on some CD1c + mDCs. Representative dot plots showing identification of CD1c + mDCs (green gate) from PBMCs of a healthy donor were illustrated in supplemental Figure 8. No HLA-DQB2 expression was detectable on CD1c + mDCs from PBMCs of a healthy donor. (D) Genomic DNA from unsorted and sorted cells from PBMCs of high-risk LCH patients (N = 3) with BRAF V600E + lesions was isolated and amplified, and the percentage of cells with BRAF V600E allele was determined by qPCR. As demonstrated in previous studies, many lineages have the potential to carry the BRAF Technical duplicates were used in this experiment. Blue dot, Patient LCH 0019; red dot, patient LCH 0020; green dot, patient LCH 0021. (E) Genomic DNA from CD1c + mDCs (HLA-DQB2 - and HLA-DQB2 + ) from PBMCs of high-risk LCH patients (N = 3) was isolated and amplified, and the percentage of cells with BRAF V600E allele was determined by qPCR. BRAF V600E was highly enriched in the HLA-DQB2 + CD1c + mDC population. Technical duplicates were used in this experiment. Blue dot, Patient LCH 0019; red dot, patient LCH 0020; green dot, patient LCH 0021.
Techniques Used: Expressing, Isolation, Amplification, Real-time Polymerase Chain Reaction
2) Product Images from "scDual-Seq: mapping the gene regulatory program of Salmonella infection by host and pathogen single-cell RNA-sequencing"
Article Title: scDual-Seq: mapping the gene regulatory program of Salmonella infection by host and pathogen single-cell RNA-sequencing
Journal: Genome Biology
Figure Legend Snippet: A single-cell RNA-sequencing approach to studying host–pathogen interaction. a Heterogeneity of outcomes of intracellular infection is due to both Salmonella and macrophage states. scDual-Seq simultaneously produces the transcriptome of both the host and the pathogen and allows the identification of cellular subpopulations during infection. b Schematic of the scDual-Seq method. Reverse transcription is primed using random hexamers, followed by RNase treatment and 3’ polyA tailing. The second strand is synthesized using the CEL-Seq2 barcoded primers (see “ Methods ”). The samples are pooled together before the complementary DNA (cDNA) undergoes linear amplification by in vitro transcription. The amplified RNA is then reverse transcribed using a random primer with an overhang of the sequence complementary to the Illumina 3’ adaptor. cDNA with both Illumina adaptors are selected by polymerase chain reaction and the DNA library is sequenced using paired-end Illumina sequencing. c Mean number of unique transcripts identified across five technical replicates, for mouse ( black ) and Salmonella ( red ). Circles and error bars represent the mean and standard deviation. d Plot between the expression of the two technical replicates of 10 pg mouse RNA and 10 pg Salmonella RNA. e Boxplots indicating the correlation coefficients across replicates with the sum expression of all 20 samples for mouse and for five replicates in each dilution for Salmonella . Mouse indicated in black , Salmonella dilutions indicated in red
Techniques Used: RNA Sequencing Assay, Infection, Synthesized, Amplification, In Vitro, Sequencing, Polymerase Chain Reaction, Standard Deviation, Expressing
Article Title: An annual cycle of gene regulation in the red-legged salamander mental gland: from hypertrophy to expression of rapidly evolving pheromones
Article Snippet: ..
Article Title: Transcriptome Analysis of Carbohydrate Metabolism Genes and Molecular Regulation of Sucrose Transport Gene LoSUT on the Flowering Process of Developing Oriental Hybrid Lily ‘Sorbonne’ Bulb
Article Snippet: A total of 60 µg RNA was pooled from all the samples equally for cDNA preparation.