complementary dna cdna synthesis  (Millipore)


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    Name:
    Gene Synthesis
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    Gene Synthesis is currently only available to our customers in Europe and some areas in Asia Please visit this page for more information about our gene synthesis services provided in Europe and some areas of Asia by GENEWIZ All other customers please join our mailing list in order to get updates on future availability in your region
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    Structured Review

    Millipore complementary dna cdna synthesis
    Gene Synthesis is currently only available to our customers in Europe and some areas in Asia Please visit this page for more information about our gene synthesis services provided in Europe and some areas of Asia by GENEWIZ All other customers please join our mailing list in order to get updates on future availability in your region
    https://www.bioz.com/result/complementary dna cdna synthesis/product/Millipore
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    complementary dna cdna synthesis - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: NMR Evidence for Forming Highly Populated Helical Conformations in the Partially Folded hNck2 SH3 Domain
    Article Snippet: .. To achieve high-level protein expression, the DNA fragment encoding the first hNck2 SH3 domain with Escherichia coli -preferred codons was obtained by a polymerase chain reaction-based de novo gene synthesis approach, as previously described ( , ), and subsequently cloned into pET32a vector (Novagen, Singapore). .. Similarly, the DNA fragment for the SH3 domain carrying four-residue Ala-mutations over residues Lue25 -Trp26 -Leu27 -Leu28 (designated 4AlaMut) was synthesized by the same approach, except that the two middle primers were replaced by those with the mutations.

    Article Title: Engineering the “Missing Link” in Biosynthetic (−)-Menthol Production: Bacterial Isopulegone Isomerase
    Article Snippet: .. Eight overlapping DNA oligonucleotides (up to 80 nt in length) were used to assemble the full length genes using the SpeedyGenes gene synthesis method ( in the Supporting Information)., Purified genes were ligated into linearized pET21b (Novagen) to generate a C-terminally His6 -tagged enzyme by In-Fusion cloning (Clontech), according to the manufacturer’s protocol. .. Cloning products were transformed into T7 Express competent E. coli cells and grown on LB agar plates containing 100 μg/mL ampicillin, followed by incubation overnight at 37 °C.

    Positron Emission Tomography:

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: .. Genes for VH expression were created using polymerase chain reaction-based gene synthesis ( ) and ligated into a pET-17b bacterial expression vector (Novagen) between the NdeI and XhoI restriction sites. .. Restriction sites for BamHI and NotI were inserted at the edges of the CDR3 loop for introducing different sequences via ligation of synthetic primers.

    Molecular Cloning:

    Article Title: Improved Biodistribution and Extended Serum Half-Life of a Bacteriophage Endolysin by Albumin Binding Domain Fusion
    Article Snippet: .. Plasmid and DNA Construct Design Gene synthesis and subcloning into the pET21a(+) vector (Novagen, Darmstadt, Germany) were performed by BioBasic Inc. (Markham, ON, Canada), and modifications resulting in LysK variants without Amidase-2 domain and Lys6-tag were performed in-house using standard molecular cloning techniques ( ). .. The ABD used in this study (ABD035) is an affinity-matured variant ( ) from streptococcal protein G ( ; ).

    Subcloning:

    Article Title: Improved Biodistribution and Extended Serum Half-Life of a Bacteriophage Endolysin by Albumin Binding Domain Fusion
    Article Snippet: .. Plasmid and DNA Construct Design Gene synthesis and subcloning into the pET21a(+) vector (Novagen, Darmstadt, Germany) were performed by BioBasic Inc. (Markham, ON, Canada), and modifications resulting in LysK variants without Amidase-2 domain and Lys6-tag were performed in-house using standard molecular cloning techniques ( ). .. The ABD used in this study (ABD035) is an affinity-matured variant ( ) from streptococcal protein G ( ; ).

    Construct:

    Article Title: Improved Biodistribution and Extended Serum Half-Life of a Bacteriophage Endolysin by Albumin Binding Domain Fusion
    Article Snippet: .. Plasmid and DNA Construct Design Gene synthesis and subcloning into the pET21a(+) vector (Novagen, Darmstadt, Germany) were performed by BioBasic Inc. (Markham, ON, Canada), and modifications resulting in LysK variants without Amidase-2 domain and Lys6-tag were performed in-house using standard molecular cloning techniques ( ). .. The ABD used in this study (ABD035) is an affinity-matured variant ( ) from streptococcal protein G ( ; ).

    Purification:

    Article Title: Engineering the “Missing Link” in Biosynthetic (−)-Menthol Production: Bacterial Isopulegone Isomerase
    Article Snippet: .. Eight overlapping DNA oligonucleotides (up to 80 nt in length) were used to assemble the full length genes using the SpeedyGenes gene synthesis method ( in the Supporting Information)., Purified genes were ligated into linearized pET21b (Novagen) to generate a C-terminally His6 -tagged enzyme by In-Fusion cloning (Clontech), according to the manufacturer’s protocol. .. Cloning products were transformed into T7 Express competent E. coli cells and grown on LB agar plates containing 100 μg/mL ampicillin, followed by incubation overnight at 37 °C.

    SYBR Green Assay:

    Article Title: Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis
    Article Snippet: .. Real-time gene synthesis was conducted with 20 µl of reaction mixture including 1× PCR buffer (Novagen), 1 µl of 0.25× to 5× SYBR Green I (1× = 1/20 000 dilution; Invitrogen) or LCGreen I (Idaho Technology Inc.), 4 mM of MgSO4 , 1 mM each of dNTP (Stratagene), 500 µg/ml of bovine serum albumin (BSA), 5–80 nM of oligonucleotides, 60 nM to 1 µM of forward and reverse primers and 1 U of KOD Hot Start (Novagen). .. The PCR were conducted under the following conditions: 2 min of initial denaturation at 95°C; 20 cycles of 95°C for 5 s, 58–70°C for 10 s, 72°C for 30 s; followed by 20 cycles of 95°C for 5 s, 49°C for 10 s, 72°C for 30 s; and final extension at 72°C for 10 min. Desalted oligonucleotides were purchased from Research Biolabs (Singapore) and Proligo (Singapore) without additional purification.

    Expressing:

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: .. Genes for VH expression were created using polymerase chain reaction-based gene synthesis ( ) and ligated into a pET-17b bacterial expression vector (Novagen) between the NdeI and XhoI restriction sites. .. Restriction sites for BamHI and NotI were inserted at the edges of the CDR3 loop for introducing different sequences via ligation of synthetic primers.

    Article Title: NMR Evidence for Forming Highly Populated Helical Conformations in the Partially Folded hNck2 SH3 Domain
    Article Snippet: .. To achieve high-level protein expression, the DNA fragment encoding the first hNck2 SH3 domain with Escherichia coli -preferred codons was obtained by a polymerase chain reaction-based de novo gene synthesis approach, as previously described ( , ), and subsequently cloned into pET32a vector (Novagen, Singapore). .. Similarly, the DNA fragment for the SH3 domain carrying four-residue Ala-mutations over residues Lue25 -Trp26 -Leu27 -Leu28 (designated 4AlaMut) was synthesized by the same approach, except that the two middle primers were replaced by those with the mutations.

    Polymerase Chain Reaction:

    Article Title: Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis
    Article Snippet: .. Real-time gene synthesis was conducted with 20 µl of reaction mixture including 1× PCR buffer (Novagen), 1 µl of 0.25× to 5× SYBR Green I (1× = 1/20 000 dilution; Invitrogen) or LCGreen I (Idaho Technology Inc.), 4 mM of MgSO4 , 1 mM each of dNTP (Stratagene), 500 µg/ml of bovine serum albumin (BSA), 5–80 nM of oligonucleotides, 60 nM to 1 µM of forward and reverse primers and 1 U of KOD Hot Start (Novagen). .. The PCR were conducted under the following conditions: 2 min of initial denaturation at 95°C; 20 cycles of 95°C for 5 s, 58–70°C for 10 s, 72°C for 30 s; followed by 20 cycles of 95°C for 5 s, 49°C for 10 s, 72°C for 30 s; and final extension at 72°C for 10 min. Desalted oligonucleotides were purchased from Research Biolabs (Singapore) and Proligo (Singapore) without additional purification.

    Plasmid Preparation:

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: .. Genes for VH expression were created using polymerase chain reaction-based gene synthesis ( ) and ligated into a pET-17b bacterial expression vector (Novagen) between the NdeI and XhoI restriction sites. .. Restriction sites for BamHI and NotI were inserted at the edges of the CDR3 loop for introducing different sequences via ligation of synthetic primers.

    Article Title: NMR Evidence for Forming Highly Populated Helical Conformations in the Partially Folded hNck2 SH3 Domain
    Article Snippet: .. To achieve high-level protein expression, the DNA fragment encoding the first hNck2 SH3 domain with Escherichia coli -preferred codons was obtained by a polymerase chain reaction-based de novo gene synthesis approach, as previously described ( , ), and subsequently cloned into pET32a vector (Novagen, Singapore). .. Similarly, the DNA fragment for the SH3 domain carrying four-residue Ala-mutations over residues Lue25 -Trp26 -Leu27 -Leu28 (designated 4AlaMut) was synthesized by the same approach, except that the two middle primers were replaced by those with the mutations.

    Article Title: Improved Biodistribution and Extended Serum Half-Life of a Bacteriophage Endolysin by Albumin Binding Domain Fusion
    Article Snippet: .. Plasmid and DNA Construct Design Gene synthesis and subcloning into the pET21a(+) vector (Novagen, Darmstadt, Germany) were performed by BioBasic Inc. (Markham, ON, Canada), and modifications resulting in LysK variants without Amidase-2 domain and Lys6-tag were performed in-house using standard molecular cloning techniques ( ). .. The ABD used in this study (ABD035) is an affinity-matured variant ( ) from streptococcal protein G ( ; ).

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  • 94
    Millipore cdna synthesis
    Expression levels of the porin gene between uninfected and infected nymphal and female ticks. (A) Porin gene expression level in B. microti -infected or -uninfected nymphal H. longicornis . (B) Gel electrophoresis analysis of conventional <t>PCR</t> products of Babesia β- tubulin (1,341 bp) in B. microti -infected and -uninfected female ticks at engorgement. Lane 1, infected tick sample; lane 2, positive control; lane 3, uninfected tick sample. (C) SSH analysis of porin expression levels in infected ticks. No or weak bands were visualized on a gel for PCR products of porin amplified from <t>cDNA</t> of infected ticks subtracted with that of uninfected ticks. Bright bands were visualized on a gel for PCR products of porin amplified from cDNA of uninfected ticks subtracted with that of infected ticks. Lanes 1, 5, 9, and 13: PCR products amplified by 18 cycles from porin gene; lanes 2, 6, 10, and 14: PCR products amplified by 23 cycles; lanes 3, 7, 11, and 15: PCR products amplified by 28 cycles; lanes 4, 8, 12, and 16: PCR products amplified by 33 cycles. (D) Real-time PCR analysis of porin mRNA expression in whole body of B. microti -infected and -uninfected engorged female ticks. The bar indicates the median with 95% CI of three biological repeats. The asterisks above the bar indicate a significant difference in porin / GAPDH between uninfected and B. microti -infected ticks (** p
    Cdna Synthesis, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/Millipore
    Average 94 stars, based on 229 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2020-08
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    97
    Millipore first strand cdna templates
    Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) <t>qRT-PCR</t> analysis of <t>cDNA</t> prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p
    First Strand Cdna Templates, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna templates/product/Millipore
    Average 97 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    85
    Millipore label first strand cdna
    Expression patterns of 25 chromosome 6-encoded genes in 10 breast cancer cell lines and 5 cases of breast cancer tumor tissues compared with the chromosome 6-mediated suppressed non-tumorigenic and non-metastatic breast cancer cell line MDA/H6. The gene expression ratio between MDA-MB-231 and MDA/H6 were derived from 12 microarray images with <t>Cy3</t> and Cy5 dye sways in <t>cDNA</t> labeling, while the ratio between others and MDA/H6 were from the duplicate microarrays. Cytobands indicate chromosome 6 band regions of the resultant genes. The color map indicates fold changes in expression ratios. LOH: loss of heterozygosity was based on the compiled data from the PUBMED literature. Yellow color indicates the highest frequency in LOH documented in literature; EBP: enhancer-binding protein.
    Label First Strand Cdna, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    Image Search Results


    Expression levels of the porin gene between uninfected and infected nymphal and female ticks. (A) Porin gene expression level in B. microti -infected or -uninfected nymphal H. longicornis . (B) Gel electrophoresis analysis of conventional PCR products of Babesia β- tubulin (1,341 bp) in B. microti -infected and -uninfected female ticks at engorgement. Lane 1, infected tick sample; lane 2, positive control; lane 3, uninfected tick sample. (C) SSH analysis of porin expression levels in infected ticks. No or weak bands were visualized on a gel for PCR products of porin amplified from cDNA of infected ticks subtracted with that of uninfected ticks. Bright bands were visualized on a gel for PCR products of porin amplified from cDNA of uninfected ticks subtracted with that of infected ticks. Lanes 1, 5, 9, and 13: PCR products amplified by 18 cycles from porin gene; lanes 2, 6, 10, and 14: PCR products amplified by 23 cycles; lanes 3, 7, 11, and 15: PCR products amplified by 28 cycles; lanes 4, 8, 12, and 16: PCR products amplified by 33 cycles. (D) Real-time PCR analysis of porin mRNA expression in whole body of B. microti -infected and -uninfected engorged female ticks. The bar indicates the median with 95% CI of three biological repeats. The asterisks above the bar indicate a significant difference in porin / GAPDH between uninfected and B. microti -infected ticks (** p

    Journal: Frontiers in Physiology

    Article Title: Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti

    doi: 10.3389/fphys.2020.00502

    Figure Lengend Snippet: Expression levels of the porin gene between uninfected and infected nymphal and female ticks. (A) Porin gene expression level in B. microti -infected or -uninfected nymphal H. longicornis . (B) Gel electrophoresis analysis of conventional PCR products of Babesia β- tubulin (1,341 bp) in B. microti -infected and -uninfected female ticks at engorgement. Lane 1, infected tick sample; lane 2, positive control; lane 3, uninfected tick sample. (C) SSH analysis of porin expression levels in infected ticks. No or weak bands were visualized on a gel for PCR products of porin amplified from cDNA of infected ticks subtracted with that of uninfected ticks. Bright bands were visualized on a gel for PCR products of porin amplified from cDNA of uninfected ticks subtracted with that of infected ticks. Lanes 1, 5, 9, and 13: PCR products amplified by 18 cycles from porin gene; lanes 2, 6, 10, and 14: PCR products amplified by 23 cycles; lanes 3, 7, 11, and 15: PCR products amplified by 28 cycles; lanes 4, 8, 12, and 16: PCR products amplified by 33 cycles. (D) Real-time PCR analysis of porin mRNA expression in whole body of B. microti -infected and -uninfected engorged female ticks. The bar indicates the median with 95% CI of three biological repeats. The asterisks above the bar indicate a significant difference in porin / GAPDH between uninfected and B. microti -infected ticks (** p

    Article Snippet: RNA extraction, cDNA synthesis, and real-time PCR were performed as described elsewhere ( ).

    Techniques: Expressing, Infection, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Positive Control, Amplification, Real-time Polymerase Chain Reaction

    Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) qRT-PCR analysis of cDNA prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p

    Journal: Journal of Translational Medicine

    Article Title: An RNA editing fingerprint of cancer stem cell reprogramming

    doi: 10.1186/s12967-014-0370-3

    Figure Lengend Snippet: Identification of an RNA editing fingerprint of malignant progenitor reprogramming, and stable ADAR1 overexpression in K562 cells. (A) LSC purification strategy for detection of CSC-associated RNA recoding. (B-D) RNA-sequencing analysis of FACS-purified CP and BC CML LSC [ 1 ] showing A-to-G RNA editing changes in MDM2, AZIN1 and APOBEC3D (n = 8 per group). (E) Lentiviral construct expressing human ADAR1 or a catalytically inactive form (ADAR1m). (F-H) qRT-PCR analysis of cDNA prepared from K562 lines using primers detecting ADAR1 lentivirus (F) and total human ADAR1 (G,H) showing K562 leukemia cells stably transduced with active ADAR1 or inactive ADAR1m express high levels of ADAR1 transcripts compared with vector open reading frame (ORF) control backbone. *p

    Article Snippet: High-fidelity PCR and Sanger sequencing analysis For PCR and targeted Sanger sequencing analysis, 1-2 μL of first-strand cDNA templates were prepared for PCR in 25-50 μL reaction volumes using the high-fidelity KOD Hot Start DNA Polymerase kit according to the manufacturer’s instructions (EMD Millipore, Temecula, CA).

    Techniques: Over Expression, Purification, RNA Sequencing Assay, FACS, Construct, Expressing, Quantitative RT-PCR, Stable Transfection, Transduction, Plasmid Preparation

    Expression patterns of 25 chromosome 6-encoded genes in 10 breast cancer cell lines and 5 cases of breast cancer tumor tissues compared with the chromosome 6-mediated suppressed non-tumorigenic and non-metastatic breast cancer cell line MDA/H6. The gene expression ratio between MDA-MB-231 and MDA/H6 were derived from 12 microarray images with Cy3 and Cy5 dye sways in cDNA labeling, while the ratio between others and MDA/H6 were from the duplicate microarrays. Cytobands indicate chromosome 6 band regions of the resultant genes. The color map indicates fold changes in expression ratios. LOH: loss of heterozygosity was based on the compiled data from the PUBMED literature. Yellow color indicates the highest frequency in LOH documented in literature; EBP: enhancer-binding protein.

    Journal: Journal of Cancer

    Article Title: Undetectable and Decreased Expression of KIAA1949 (Phostensin) Encoded on Chromosome 6p21.33 in Human Breast Cancers Revealed by Transcriptome Analysis

    doi:

    Figure Lengend Snippet: Expression patterns of 25 chromosome 6-encoded genes in 10 breast cancer cell lines and 5 cases of breast cancer tumor tissues compared with the chromosome 6-mediated suppressed non-tumorigenic and non-metastatic breast cancer cell line MDA/H6. The gene expression ratio between MDA-MB-231 and MDA/H6 were derived from 12 microarray images with Cy3 and Cy5 dye sways in cDNA labeling, while the ratio between others and MDA/H6 were from the duplicate microarrays. Cytobands indicate chromosome 6 band regions of the resultant genes. The color map indicates fold changes in expression ratios. LOH: loss of heterozygosity was based on the compiled data from the PUBMED literature. Yellow color indicates the highest frequency in LOH documented in literature; EBP: enhancer-binding protein.

    Article Snippet: Briefly, 50-μg total RNA was mixed with Cy3-dUTP (or Cy5-dUTP) and other reagents from the kit to synthesize the label first strand cDNA at 42ºC for 1 h. The reaction was stopped by addition of 2.5-μl 0.5M EDTA and 2.5-μl 1N NaOH and then incubated at 65ºC for 30 min. After adding 6.2-μl 1M Tris-HCl (pH 7.5), the samples were purified by use of Microcon 100 (Cat. No. 42412, Millipore Corp., Bedford, MA) to remove unincorporated nucleotides and salts.

    Techniques: Expressing, Multiple Displacement Amplification, Derivative Assay, Microarray, Labeling, Binding Assay