complementary dna cdna synthesis kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher complementary dna cdna synthesis kit
    Complementary Dna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary dna cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    complementary dna cdna synthesis kit - by Bioz Stars, 2020-05
    99/100 stars

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    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Kupffer Cell Suppression of CD8+ T cells in Human Hepatocellular Carcinoma is Mediated by B7-H1/PD-1 Interactions
    Article Snippet: .. For RT-PCR, total RNA from tumor tissues and liver tissue as indicated were isolated using RNeasy Kits (QIAGEN, Valencia, CA). cDNA synthesis was performed using SuperScript One-Cycle cDNA Kit (Invitrogen). .. The cDNA served as a template in Real-Time PCR using Fast SYBR Green Master Kit (Applied Biosystems, Foster City, CA).

    Article Title: Longitudinal fluctuations in PD1 and PD-L1 expression in association with changes in anti-viral immune response in chronic hepatitis B
    Article Snippet: .. For RT-PCR, total RNA from liver biopsy tissue as indicated was isolated using RNeasy kits (Qiagen, Venlo, Netherlands). cDNA synthesis was performed using SuperScript One-Cycle cDNA kit (Life Technologies, Carlsbad, CA USA). .. The cDNA served as a template for real-time PCR using Fast SYBR Green Master kit (Life Technologies).

    Synthesized:

    Article Title: Fuzzy logic selection as a new reliable tool to identify molecular grade signatures in breast cancer – the INNODIAG study
    Article Snippet: .. Double-stranded cDNA (ds-cDNA) was synthesized from 2 μg of total RNA using SuperScript One cycle kit (Invitrogen Life Technologies, Saint-Aubin, France) with random primers and Oligo(dT) primers, then cleaned with MinElute PCR Purification Kit (Qiagen, Courtaboeuf, France). .. ERCC RNA Spike-In Control Mix (Ambion Life technologies, Saint-Aubin, France), a set of external RNA positive controls, was added to total RNA at the beginning of the experiment to assess accuracy of measurements of gene expression.

    Isolation:

    Article Title: Kupffer Cell Suppression of CD8+ T cells in Human Hepatocellular Carcinoma is Mediated by B7-H1/PD-1 Interactions
    Article Snippet: .. For RT-PCR, total RNA from tumor tissues and liver tissue as indicated were isolated using RNeasy Kits (QIAGEN, Valencia, CA). cDNA synthesis was performed using SuperScript One-Cycle cDNA Kit (Invitrogen). .. The cDNA served as a template in Real-Time PCR using Fast SYBR Green Master Kit (Applied Biosystems, Foster City, CA).

    Article Title: Longitudinal fluctuations in PD1 and PD-L1 expression in association with changes in anti-viral immune response in chronic hepatitis B
    Article Snippet: .. For RT-PCR, total RNA from liver biopsy tissue as indicated was isolated using RNeasy kits (Qiagen, Venlo, Netherlands). cDNA synthesis was performed using SuperScript One-Cycle cDNA kit (Life Technologies, Carlsbad, CA USA). .. The cDNA served as a template for real-time PCR using Fast SYBR Green Master kit (Life Technologies).

    Construct:

    Article Title: Sex-biased gene expression in the brown alga Fucus vesiculosus
    Article Snippet: .. Double stranded cDNA was constructed using the SuperScript® One-Cycle cDNA kit (Invitrogen), following the manufacturer’s instructions. ..

    Purification:

    Article Title: Fuzzy logic selection as a new reliable tool to identify molecular grade signatures in breast cancer – the INNODIAG study
    Article Snippet: .. Double-stranded cDNA (ds-cDNA) was synthesized from 2 μg of total RNA using SuperScript One cycle kit (Invitrogen Life Technologies, Saint-Aubin, France) with random primers and Oligo(dT) primers, then cleaned with MinElute PCR Purification Kit (Qiagen, Courtaboeuf, France). .. ERCC RNA Spike-In Control Mix (Ambion Life technologies, Saint-Aubin, France), a set of external RNA positive controls, was added to total RNA at the beginning of the experiment to assess accuracy of measurements of gene expression.

    Microarray:

    Article Title: Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae
    Article Snippet: .. RNA for microarray analysis was reverse transcribed using a SuperScript One-Cycle cDNA Kit (Invitrogen) as outlined in the NimbleGen Microarray Experienced User’s Guide including RNaseA and cDNA precipitation steps. .. RNA for qRT-PCR was reverse transcribed using a Roche Transcriptor First Strand cDNA Synthesis kit with random hexamers.

    Article Title: Development and Validation of an Haemophilus influenzae Supragenome Hybridization (SGH) Array for Transcriptomic Analyses
    Article Snippet: .. Generation of labelled double-stranded cDNA for SGH array hybridization First and second-strand cDNA synthesis was performed using a SuperScript One-Cycle cDNA Kit (Invitrogen) as outlined in the NimbleGen Microarray Experienced User’s Guide including RNaseA and cDNA precipitation steps. .. 1 µg of cDNA was Cy3-labeled using a NimbleGen One-Color DNA Labeling Kit.

    Polymerase Chain Reaction:

    Article Title: Fuzzy logic selection as a new reliable tool to identify molecular grade signatures in breast cancer – the INNODIAG study
    Article Snippet: .. Double-stranded cDNA (ds-cDNA) was synthesized from 2 μg of total RNA using SuperScript One cycle kit (Invitrogen Life Technologies, Saint-Aubin, France) with random primers and Oligo(dT) primers, then cleaned with MinElute PCR Purification Kit (Qiagen, Courtaboeuf, France). .. ERCC RNA Spike-In Control Mix (Ambion Life technologies, Saint-Aubin, France), a set of external RNA positive controls, was added to total RNA at the beginning of the experiment to assess accuracy of measurements of gene expression.

    Hybridization:

    Article Title: Development and Validation of an Haemophilus influenzae Supragenome Hybridization (SGH) Array for Transcriptomic Analyses
    Article Snippet: .. Generation of labelled double-stranded cDNA for SGH array hybridization First and second-strand cDNA synthesis was performed using a SuperScript One-Cycle cDNA Kit (Invitrogen) as outlined in the NimbleGen Microarray Experienced User’s Guide including RNaseA and cDNA precipitation steps. .. 1 µg of cDNA was Cy3-labeled using a NimbleGen One-Color DNA Labeling Kit.

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    Thermo Fisher taqman advanced mirna cdna synthesis kit
    Real-time PCR validation of Cd-dysregulated miRNAs. <t>TaqMan</t> ® Advanced <t>miRNA</t> assays were used to validate selected Cd-dysregulated miRNAs. ( A ) miR-21-5p; ( B ) miR-34a-5p; ( C ) miR-146b-5p; ( D ) miR-224-5p; ( E ) miR-3084a-3p; ( F ) miR-3084c-3p; ( G ) miR-455-3p; ( H ) miR-423-5p. The comparative CT method was used to determine the fold change (±SEM), and an asterisk (*) indicates a statistically significant change in expression in the Cd-treated group versus the saline control ( N = 6 per group, unpaired t -test, p ≤ 0.05).
    Taqman Advanced Mirna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman advanced mirna cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    taqman advanced mirna cdna synthesis kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher first strand cdna synthesis kit
    Elevation of miR-99a-5p expression levels in 5637 and T24 cells transfected with pSM-99a. Notes: ( A ) miR-99a-5p was downregulated in 5637 and T24 cells. Cells at 70% confluence were collected, and the expression of miR-99a-5p was detected by miRNA QPCR. ( B and C ) Transfection of pSM-99a elevated the expression level of miR-99a-5p in 5637 and T24 cells. Transfected cells were selected with 200 μg/mL G418 for 24 h, while co-expressed EGFP was detectable under fluorescent microscopy. Total <t>RNA</t> was extracted and reverse transcribed, and the resulting first <t>cDNA</t> was applied to the subsequent miRNA QPCR. miR-99a-5p expression was normalized by U6 RNA. Data represent three independent experiments and are expressed as fold ± SD relative to the SV-Huc1 or vector only control (pSM). * P
    First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 1499 article reviews
    Price from $9.99 to $1999.99
    first strand cdna synthesis kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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    Real-time PCR validation of Cd-dysregulated miRNAs. TaqMan ® Advanced miRNA assays were used to validate selected Cd-dysregulated miRNAs. ( A ) miR-21-5p; ( B ) miR-34a-5p; ( C ) miR-146b-5p; ( D ) miR-224-5p; ( E ) miR-3084a-3p; ( F ) miR-3084c-3p; ( G ) miR-455-3p; ( H ) miR-423-5p. The comparative CT method was used to determine the fold change (±SEM), and an asterisk (*) indicates a statistically significant change in expression in the Cd-treated group versus the saline control ( N = 6 per group, unpaired t -test, p ≤ 0.05).

    Journal: Toxics

    Article Title: Cadmium Nephrotoxicity Is Associated with Altered MicroRNA Expression in the Rat Renal Cortex

    doi: 10.3390/toxics6010016

    Figure Lengend Snippet: Real-time PCR validation of Cd-dysregulated miRNAs. TaqMan ® Advanced miRNA assays were used to validate selected Cd-dysregulated miRNAs. ( A ) miR-21-5p; ( B ) miR-34a-5p; ( C ) miR-146b-5p; ( D ) miR-224-5p; ( E ) miR-3084a-3p; ( F ) miR-3084c-3p; ( G ) miR-455-3p; ( H ) miR-423-5p. The comparative CT method was used to determine the fold change (±SEM), and an asterisk (*) indicates a statistically significant change in expression in the Cd-treated group versus the saline control ( N = 6 per group, unpaired t -test, p ≤ 0.05).

    Article Snippet: The cDNA template for PCR was prepared using 10 ng of total RNA sample and the TaqMan® Advanced miRNA cDNA Synthesis kit (Thermo Fisher Scientific) following the manufacturer’s recommended protocol.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    circRNA effects on linear transcripts, miRNAs and tumor growth ( A ) Upon efficient transduction of TUSC3 circ104557 in HCT-116 cells (harboring a methylated CpG island), the TUSC3 linear RNA levels did not change: it was not detected in any of the conditions tested. ( B ) TUSC3 circ104557 transduction in DKO cells (harboring an unmethylated CpG island) did not affect the levels of TUSC3 linear RNA. RNA levels were determined using circular or linear specific qRT-PCR primers. The lentiviral transduction of the empty vector (Mock condition) was used as a control. Experiments were performed in technical triplicates. ( C ) Expression of candidate miRNAs putatively targeted by ATRNL1 circ100686 (miR-378a-3p), SAMD4A circ101356 (miR-660-5p and miR-330-3p) and TUSC3 circ104557 (miR-330-3p) was significantly downregulated in DKO cells, evaluated in three biological replicates by real-time quantitative PCR using TaqMan Advanced MicroRNA Assays. Expression levels were normalized using hsa-miR-345-5p, hsa-miR-191-5p and hsa-miR-423-3p advanced Control miRNA Assays. ( D ) Using the same strategy, expression of miR-330-3p, putatively targeted by TUSC3 circ104557, was also assessed in the gain-of-function cellular model. A significant downregulation of miR-330-3p was detected upon TUSC3 circ104557 transduction in HCT116 cells. ( E ) HCT116-Mock and HCT116-TUSC3 circular cells were injected in the left or right flank of 10 mice, respectively. Tumor volume measured over time (left panel) and tumor weight upon sacrifice (right panel) are shown. Tumor growth was significantly reduced upon TUSC3 circular ectopic expression. ns, nonsignificant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001, using Student’s t -test. Error bars show means ± s.d.

    Journal: Oncotarget

    Article Title: Circular RNA CpG island hypermethylation-associated silencing in human cancer

    doi: 10.18632/oncotarget.25673

    Figure Lengend Snippet: circRNA effects on linear transcripts, miRNAs and tumor growth ( A ) Upon efficient transduction of TUSC3 circ104557 in HCT-116 cells (harboring a methylated CpG island), the TUSC3 linear RNA levels did not change: it was not detected in any of the conditions tested. ( B ) TUSC3 circ104557 transduction in DKO cells (harboring an unmethylated CpG island) did not affect the levels of TUSC3 linear RNA. RNA levels were determined using circular or linear specific qRT-PCR primers. The lentiviral transduction of the empty vector (Mock condition) was used as a control. Experiments were performed in technical triplicates. ( C ) Expression of candidate miRNAs putatively targeted by ATRNL1 circ100686 (miR-378a-3p), SAMD4A circ101356 (miR-660-5p and miR-330-3p) and TUSC3 circ104557 (miR-330-3p) was significantly downregulated in DKO cells, evaluated in three biological replicates by real-time quantitative PCR using TaqMan Advanced MicroRNA Assays. Expression levels were normalized using hsa-miR-345-5p, hsa-miR-191-5p and hsa-miR-423-3p advanced Control miRNA Assays. ( D ) Using the same strategy, expression of miR-330-3p, putatively targeted by TUSC3 circ104557, was also assessed in the gain-of-function cellular model. A significant downregulation of miR-330-3p was detected upon TUSC3 circ104557 transduction in HCT116 cells. ( E ) HCT116-Mock and HCT116-TUSC3 circular cells were injected in the left or right flank of 10 mice, respectively. Tumor volume measured over time (left panel) and tumor weight upon sacrifice (right panel) are shown. Tumor growth was significantly reduced upon TUSC3 circular ectopic expression. ns, nonsignificant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001, using Student’s t -test. Error bars show means ± s.d.

    Article Snippet: In brief, TaqMan™ Advanced miRNA cDNA Synthesis Kit (ThermoFisher, Cat. No. ) was used for retrotranscription.

    Techniques: Transduction, Methylation, Quantitative RT-PCR, Plasmid Preparation, Expressing, Real-time Polymerase Chain Reaction, Injection, Mouse Assay

    Percentage of detection for each miRNA, employing ( a ) Taqman qPCR or ( b ) Multiplex Circulating miRNA Assay (Abcam).

    Journal: Cancers

    Article Title: Comparative miRNA Analysis of Urine Extracellular Vesicles Isolated through Five Different Methods

    doi: 10.3390/cancers8120112

    Figure Lengend Snippet: Percentage of detection for each miRNA, employing ( a ) Taqman qPCR or ( b ) Multiplex Circulating miRNA Assay (Abcam).

    Article Snippet: Taqman miRNA Assay Reverse transcription of the miRNA was performed using TaqMan Advanced miRNA cDNA Synthesis Kit (ThermoFisher Scientific), following the manufacturer’s recommendations.

    Techniques: Real-time Polymerase Chain Reaction, Multiplex Assay

    Correlation between signal intensity (Log 10 transformed) achieved by multiplex miRNA assay and C t values obtained by Taqman qPCR, using the same RNAs.

    Journal: Cancers

    Article Title: Comparative miRNA Analysis of Urine Extracellular Vesicles Isolated through Five Different Methods

    doi: 10.3390/cancers8120112

    Figure Lengend Snippet: Correlation between signal intensity (Log 10 transformed) achieved by multiplex miRNA assay and C t values obtained by Taqman qPCR, using the same RNAs.

    Article Snippet: Taqman miRNA Assay Reverse transcription of the miRNA was performed using TaqMan Advanced miRNA cDNA Synthesis Kit (ThermoFisher Scientific), following the manufacturer’s recommendations.

    Techniques: Transformation Assay, Multiplex Assay, Real-time Polymerase Chain Reaction

    Elevation of miR-99a-5p expression levels in 5637 and T24 cells transfected with pSM-99a. Notes: ( A ) miR-99a-5p was downregulated in 5637 and T24 cells. Cells at 70% confluence were collected, and the expression of miR-99a-5p was detected by miRNA QPCR. ( B and C ) Transfection of pSM-99a elevated the expression level of miR-99a-5p in 5637 and T24 cells. Transfected cells were selected with 200 μg/mL G418 for 24 h, while co-expressed EGFP was detectable under fluorescent microscopy. Total RNA was extracted and reverse transcribed, and the resulting first cDNA was applied to the subsequent miRNA QPCR. miR-99a-5p expression was normalized by U6 RNA. Data represent three independent experiments and are expressed as fold ± SD relative to the SV-Huc1 or vector only control (pSM). * P

    Journal: OncoTargets and therapy

    Article Title: miR-99a-5p acts as tumor suppressor via targeting to mTOR and enhances RAD001-induced apoptosis in human urinary bladder urothelial carcinoma cells

    doi: 10.2147/OTT.S114276

    Figure Lengend Snippet: Elevation of miR-99a-5p expression levels in 5637 and T24 cells transfected with pSM-99a. Notes: ( A ) miR-99a-5p was downregulated in 5637 and T24 cells. Cells at 70% confluence were collected, and the expression of miR-99a-5p was detected by miRNA QPCR. ( B and C ) Transfection of pSM-99a elevated the expression level of miR-99a-5p in 5637 and T24 cells. Transfected cells were selected with 200 μg/mL G418 for 24 h, while co-expressed EGFP was detectable under fluorescent microscopy. Total RNA was extracted and reverse transcribed, and the resulting first cDNA was applied to the subsequent miRNA QPCR. miR-99a-5p expression was normalized by U6 RNA. Data represent three independent experiments and are expressed as fold ± SD relative to the SV-Huc1 or vector only control (pSM). * P

    Article Snippet: First-strand cDNA was synthesized from total RNA (2.5 μg) using First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) and oligo dT primers.

    Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, Microscopy, Plasmid Preparation