Structured Review

TaKaRa complementary dna cdna synthesis kit
Complementary Dna Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complementary dna cdna synthesis kit/product/TaKaRa
Average 99 stars, based on 2 article reviews
Price from $9.99 to $1999.99
complementary dna cdna synthesis kit - by Bioz Stars, 2020-08
99/100 stars

Images

Related Articles

Irradiation:

Article Title: Unraveling Fungal Radiation Resistance Regulatory Networks through the Genome-Wide Transcriptome and Genetic Analyses of Cryptococcus neoformans
Article Snippet: .. The cDNA was synthesized using the PrimeScript first-strand cDNA synthesis kit (TaKaRa) with total RNAs extracted from irradiated cells. ..

Synthesized:

Article Title: Ultrasmall Gold Nanoparticles as Carriers for Nucleus-Based Gene Therapy Due to Size-Dependent Nuclear Entry
Article Snippet: .. MCF-7 cells were incubated with Au-POY2T NPs and POY2T at a POY2T concentration of 5 μM for 24 h. For RT-PCR, total mRNA was extracted from MCF-7 cells, and first-strand cDNA was synthesized using a PrimeScript first-strand cDNA synthesis kit (Takara, Shiga, Japan). .. Real-time PCR analysis of c-myc was conducted for quantitative analysis of the reduction in c-myc transcription.

Article Title: Unraveling Fungal Radiation Resistance Regulatory Networks through the Genome-Wide Transcriptome and Genetic Analyses of Cryptococcus neoformans
Article Snippet: .. The cDNA was synthesized using the PrimeScript first-strand cDNA synthesis kit (TaKaRa) with total RNAs extracted from irradiated cells. ..

Article Title: Urokinase-type plasminogen activator receptor interaction with β1 integrin is required for platelet-derived growth factor-AB-induced human mesenchymal stem/stromal cell migration
Article Snippet: .. First-strand cDNA was synthesized from 1.5–2.0 μg total RNA using the PrimeScript™ first strand cDNA synthesis Kit (TaKaRa, Saint Germain en Laye, France). .. All samples were subjected to PCR amplification with oligonucleotide primers for uPAR or uPA and the constitutively expressed gene for GAPDH.

Isolation:

Article Title: Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties
Article Snippet: .. High-quality RNA was isolated for cDNA synthesis by using the PrimeScript™ first-strand cDNA synthesis kit (Takara, Japan) according to the manufacturer’s protocol. .. The primers used for real-time PCR were as follows: Forward 5′-TTGCTTCAGGGTTTCATCCAG-3′ and reverse 5′-AGCTTCTTGGTGGACGCATC-3′ for Bax , and forward 5′-TGTGGATGACTGAGTACCTGAACC-3′ and reverse 5′-CAGCCAGGAGAAATCAAACAGAG-3′ for Bcl-2 .

Quantitative RT-PCR:

Article Title: Exosome Mediated Delivery of miR-124 Promotes Neurogenesis after Ischemia
Article Snippet: .. For analysis of mRNA levels, reverse-transcription was performed using PrimeScript First-Strand cDNA Synthesis Kit (Takara), and cDNAs were used for qRT-PCR using PrimeScript RT Master Mix (Takara). .. For analysis of miRNA levels, total RNA was reverse-transcribed to cDNA using miRcute miRNA First-Strand cDNA Synthesis Kit (Tiangen Biotech), and qRT-PCR was carried out using miRcute miRNA qPCR Detection Kit (SYBR Green) (Tiangen Biotech).

Real-time Polymerase Chain Reaction:

Article Title: Beta Palmitate Improves Bone Length and Quality during Catch-Up Growth in Young Rats
Article Snippet: .. Reverse Transcription and Real Time PCR for GDF-5 To measure the expression of growth and differentiation factor 5 (GDF-5) in EGP, first-strand cDNA synthesis was performed with the PrimeScript First-Strand cDNA Synthesis Kit (Takara Bio, Mountainview, CA, USA) using 1 μg of the total RNA as a template, according to the manufacturer’s instructions. .. A real-time quantitative polymerase chain reaction (qPCR) was performed with the ABI Prism 7000 Sequence Detection System (Applied Biosystems Inc., Foster City, CA, USA), according to the manufacturer’s instructions and using specific FAM- labeled probes (TaqMan® assay on demand Rn0043356-m1 for GDF5); Rn00667086 for Aco2 served as the internal control [ ]).

Article Title: Proteomic Analysis of GLUT4 Storage Vesicles Reveals Tumor Suppressor Candidate 5 (TUSC5) as a Novel Regulator of Insulin Action in Adipocytes *
Article Snippet: .. qPCR Total RNA was extracted from cells using TRIzol reagent (Invitrogen). cDNA synthesis was carried out using PrimeScript first strand cDNA synthesis kit (Clontech and Takara Bio Company). .. PCRs were performed on the LightCycler 480 II (Roche Applied Science) using FastStart Universal SYBR Green Master (Roche Applied Science).

Concentration Assay:

Article Title: Ultrasmall Gold Nanoparticles as Carriers for Nucleus-Based Gene Therapy Due to Size-Dependent Nuclear Entry
Article Snippet: .. MCF-7 cells were incubated with Au-POY2T NPs and POY2T at a POY2T concentration of 5 μM for 24 h. For RT-PCR, total mRNA was extracted from MCF-7 cells, and first-strand cDNA was synthesized using a PrimeScript first-strand cDNA synthesis kit (Takara, Shiga, Japan). .. Real-time PCR analysis of c-myc was conducted for quantitative analysis of the reduction in c-myc transcription.

Incubation:

Article Title: Ultrasmall Gold Nanoparticles as Carriers for Nucleus-Based Gene Therapy Due to Size-Dependent Nuclear Entry
Article Snippet: .. MCF-7 cells were incubated with Au-POY2T NPs and POY2T at a POY2T concentration of 5 μM for 24 h. For RT-PCR, total mRNA was extracted from MCF-7 cells, and first-strand cDNA was synthesized using a PrimeScript first-strand cDNA synthesis kit (Takara, Shiga, Japan). .. Real-time PCR analysis of c-myc was conducted for quantitative analysis of the reduction in c-myc transcription.

Expressing:

Article Title: Beta Palmitate Improves Bone Length and Quality during Catch-Up Growth in Young Rats
Article Snippet: .. Reverse Transcription and Real Time PCR for GDF-5 To measure the expression of growth and differentiation factor 5 (GDF-5) in EGP, first-strand cDNA synthesis was performed with the PrimeScript First-Strand cDNA Synthesis Kit (Takara Bio, Mountainview, CA, USA) using 1 μg of the total RNA as a template, according to the manufacturer’s instructions. .. A real-time quantitative polymerase chain reaction (qPCR) was performed with the ABI Prism 7000 Sequence Detection System (Applied Biosystems Inc., Foster City, CA, USA), according to the manufacturer’s instructions and using specific FAM- labeled probes (TaqMan® assay on demand Rn0043356-m1 for GDF5); Rn00667086 for Aco2 served as the internal control [ ]).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Ultrasmall Gold Nanoparticles as Carriers for Nucleus-Based Gene Therapy Due to Size-Dependent Nuclear Entry
Article Snippet: .. MCF-7 cells were incubated with Au-POY2T NPs and POY2T at a POY2T concentration of 5 μM for 24 h. For RT-PCR, total mRNA was extracted from MCF-7 cells, and first-strand cDNA was synthesized using a PrimeScript first-strand cDNA synthesis kit (Takara, Shiga, Japan). .. Real-time PCR analysis of c-myc was conducted for quantitative analysis of the reduction in c-myc transcription.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    TaKaRa cdna preparation kit
    Northern analyses of a few select forward subtracted SSH <t>cDNA</t> clones . RNA isolated from the leaves of the control and 425 mM NaCl-treated plants and blotted onto Hybond N + membrane was hybridized with the individual radiolabelled ESTs. A RNA blot each for the control and NaCl-treated sample was hybridized with <t>PCR</t> amplified radiolabelled actin fragment. The horizontal bars against the individual genes represent increase (in%) in transcripts of the respective genes in response to NaCl treatment of the plant when compared to control. The values were obtained after normalization of the blot intensities of actin for the control and NaCl treated sample.
    Cdna Preparation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna preparation kit/product/TaKaRa
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna preparation kit - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    TaKaRa smart race cdna amplification kit
    <t>RACE</t> and RT-PCR analyses identify an additional upstream G50 exon. (A) RACE analyses were performed using <t>cDNA</t> generated from G50pKO-infected 3T12 or A20-HE2 cells 24 hours after treatment with TPA. The 5′ end of E0 was mapped using the primer
    Smart Race Cdna Amplification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart race cdna amplification kit/product/TaKaRa
    Average 99 stars, based on 1516 article reviews
    Price from $9.99 to $1999.99
    smart race cdna amplification kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Northern analyses of a few select forward subtracted SSH cDNA clones . RNA isolated from the leaves of the control and 425 mM NaCl-treated plants and blotted onto Hybond N + membrane was hybridized with the individual radiolabelled ESTs. A RNA blot each for the control and NaCl-treated sample was hybridized with PCR amplified radiolabelled actin fragment. The horizontal bars against the individual genes represent increase (in%) in transcripts of the respective genes in response to NaCl treatment of the plant when compared to control. The values were obtained after normalization of the blot intensities of actin for the control and NaCl treated sample.

    Journal: BMC Plant Biology

    Article Title: Isolation, identification and expression analysis of salt-induced genes in Suaeda maritima, a natural halophyte, using PCR-based suppression subtractive hybridization

    doi: 10.1186/1471-2229-9-69

    Figure Lengend Snippet: Northern analyses of a few select forward subtracted SSH cDNA clones . RNA isolated from the leaves of the control and 425 mM NaCl-treated plants and blotted onto Hybond N + membrane was hybridized with the individual radiolabelled ESTs. A RNA blot each for the control and NaCl-treated sample was hybridized with PCR amplified radiolabelled actin fragment. The horizontal bars against the individual genes represent increase (in%) in transcripts of the respective genes in response to NaCl treatment of the plant when compared to control. The values were obtained after normalization of the blot intensities of actin for the control and NaCl treated sample.

    Article Snippet: Double stranded cDNA was prepared by reverse transcription of 4 μg of the purified mRNA in 20 μl reaction solution following the steps outlined in the cDNA preparation kit (Super SMART PCR cDNA synthesis kit, Clontech, Palo alto, USA).

    Techniques: Northern Blot, Clone Assay, Isolation, Northern blot, Polymerase Chain Reaction, Amplification

    RACE and RT-PCR analyses identify an additional upstream G50 exon. (A) RACE analyses were performed using cDNA generated from G50pKO-infected 3T12 or A20-HE2 cells 24 hours after treatment with TPA. The 5′ end of E0 was mapped using the primer

    Journal:

    Article Title: Alternatively Initiated Gene 50/RTA Transcripts Expressed during Murine and Human Gammaherpesvirus Reactivation from Latency ▿

    doi: 10.1128/JVI.01444-08

    Figure Lengend Snippet: RACE and RT-PCR analyses identify an additional upstream G50 exon. (A) RACE analyses were performed using cDNA generated from G50pKO-infected 3T12 or A20-HE2 cells 24 hours after treatment with TPA. The 5′ end of E0 was mapped using the primer

    Article Snippet: For 3T12 analysis, poly(A) RNA was purified using the Sigma gel elute kit, and 300 ng was converted to cDNA using the BD Smart RACE cDNA amplification kit (Clontech).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Generated, Infection

    of the amg-miR166 cleavage sites by 5′ Rapid Amplification of cDNA Ends (RACE).

    Journal: PLoS ONE

    Article Title: Characterization of microRNAs Expressed during Secondary Wall Biosynthesis in Acacia mangium

    doi: 10.1371/journal.pone.0049662

    Figure Lengend Snippet: of the amg-miR166 cleavage sites by 5′ Rapid Amplification of cDNA Ends (RACE).

    Article Snippet: First strand cDNA was constructed using SMART™ RACE cDNA Amplification Kit (CLONTECH Laboratories, Inc. USA) following protocol instruction.

    Techniques: Rapid Amplification of cDNA Ends

    Amino acid alignment of Anisakis haemoglobin with haemoglobins of Ascaris , Pseudoterranova and Nippostrongylus brasilensis , and phylogenetic analysis. A ) The cDNA sequence of Anisakis haemoglobin was obtained by degenerate PCR and RACE-PCR (accession number: JX860676) and the amino acid sequence was deduced. Alignment of sequences was carried out using MUSCLE ( http://www.ebi.ac.uk/Tools/muscle/index.html ). The hydrophobic leader portion (sequence not obtained in Anisakis ) and the C-terminal tail (important for assembly of octamers in Ascaris ) are boxed in black, while hydrophobic haem-binding regions are boxed in red. The haemoglobin protein consists of two homologous domains, illustrated by dashed and dotted lines. The B10 tyrosine, E7 distal glutamine and F8 proximal histidine, important in binding of oxygen, are conserved between the three nematodes and are marked with boxes. B ) A range of haemoglobin sequences across different species was obtained by BLAST search. The evolutionary history was inferred using the Minimum Evolution method [21] . The bootstrap consensus tree inferred from 2000 replicates is taken to represent the evolutionary history of the taxa analyzed. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm at a search level of 0. The Neighbor-joining algorithm was used to generate the initial tree. The analysis involved 26 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. There were a total of 64 positions in the final dataset. Evolutionary analyses were conducted in MEGA5.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: A Cross-Reactive Monoclonal Antibody to Nematode Haemoglobin Enhances Protective Immune Responses to Nippostrongylus brasiliensis

    doi: 10.1371/journal.pntd.0002395

    Figure Lengend Snippet: Amino acid alignment of Anisakis haemoglobin with haemoglobins of Ascaris , Pseudoterranova and Nippostrongylus brasilensis , and phylogenetic analysis. A ) The cDNA sequence of Anisakis haemoglobin was obtained by degenerate PCR and RACE-PCR (accession number: JX860676) and the amino acid sequence was deduced. Alignment of sequences was carried out using MUSCLE ( http://www.ebi.ac.uk/Tools/muscle/index.html ). The hydrophobic leader portion (sequence not obtained in Anisakis ) and the C-terminal tail (important for assembly of octamers in Ascaris ) are boxed in black, while hydrophobic haem-binding regions are boxed in red. The haemoglobin protein consists of two homologous domains, illustrated by dashed and dotted lines. The B10 tyrosine, E7 distal glutamine and F8 proximal histidine, important in binding of oxygen, are conserved between the three nematodes and are marked with boxes. B ) A range of haemoglobin sequences across different species was obtained by BLAST search. The evolutionary history was inferred using the Minimum Evolution method [21] . The bootstrap consensus tree inferred from 2000 replicates is taken to represent the evolutionary history of the taxa analyzed. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm at a search level of 0. The Neighbor-joining algorithm was used to generate the initial tree. The analysis involved 26 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. There were a total of 64 positions in the final dataset. Evolutionary analyses were conducted in MEGA5.

    Article Snippet: Amplification of the 5′ and 3′ regions of the haemoglobin sequence was conducted using the SMART RACE CDNA Amplification Kit (Clontech) with the Primescript Reverse Transcriptase (Takara, Japan).

    Techniques: Sequencing, Polymerase Chain Reaction, Binding Assay