complementary dna cdna conversion kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher complementary dna cdna conversion kit
    Complementary Dna Cdna Conversion Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary dna cdna conversion kit/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complementary dna cdna conversion kit - by Bioz Stars, 2020-08
    93/100 stars

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    Article Title: Iron accentuated reactive oxygen species release by NADPH oxidase in activated microglia contributes to oxidative stress in vitro
    Article Snippet: A complementary DNA (cDNA) conversion kit (Applied Biosciences, Waltham, MA) used a Veriti thermal cycler (Applied Biosciences) to convert 1 μg of mRNA into cDNA, according to the manufacturer’s suggested protocol.

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    Thermo Fisher verso cdna kit
    mRNA expression of N-extended AChE, AChE-S, and AChE-R isoforms following treatment of Y79 cells with glucose for 1 h. Y79 cells were pre-treated for 16–24 h in starvation medium (1% FBS and 1 mg/ml of glucose) and then with 3.5 mg/ml glucose for different time intervals. Total <t>RNA</t> was extracted and <t>cDNA</t> was prepared for real-time PCR procedure as described under Materials and Methods. Results are presented as fold of control cells cultured in starvation media. Values are means ± SEM, ( N = 4). * p
    Verso Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/verso cdna kit/product/Thermo Fisher
    Average 99 stars, based on 266 article reviews
    Price from $9.99 to $1999.99
    verso cdna kit - by Bioz Stars, 2020-08
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    90
    Thermo Fisher cdna kit
    Differentiated adipocytes and fat depots express <t>CXCR2</t> . (A) 3T3‐L1 cells were differentiated into adipocytes and cells stained with oil red‐O (scale bars: 50 μm). (B) mRNA was extracted before and after differentiation, reverse transcribed to <t>cDNA</t> and analyzed for CXCR2 expression relative to the house‐keeping gene 18s. (C) Oil red‐O staining was quantified in undifferentiated and differentiated adipocytes in the absence or presence of two different CXCR2 inhibitors (expressed relative to differentiated cells). (D) Adipocytes differentiated in the absence and presence of a CXCR2 inhibitor 1 were analyzed for PPARγ protein expression relative to GAPDH levels (left panel) and quantified relative to GAPDH using densitometry. Data are plotted as mean (± sem ), where each symbol represents an experimental replicate. Analyzed using an unpaired t test with Welch's correction (B and D) or one‐way ANOVA with TUKEY's multiple comparison test (C). Data are representative of two separate experiments
    Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna kit/product/Thermo Fisher
    Average 90 stars, based on 269 article reviews
    Price from $9.99 to $1999.99
    cdna kit - by Bioz Stars, 2020-08
    90/100 stars
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    99
    Thermo Fisher taqman advanced mirna cdna synthesis kit
    Real-time PCR validation of Cd-dysregulated miRNAs. <t>TaqMan</t> ® Advanced <t>miRNA</t> assays were used to validate selected Cd-dysregulated miRNAs. ( A ) miR-21-5p; ( B ) miR-34a-5p; ( C ) miR-146b-5p; ( D ) miR-224-5p; ( E ) miR-3084a-3p; ( F ) miR-3084c-3p; ( G ) miR-455-3p; ( H ) miR-423-5p. The comparative CT method was used to determine the fold change (±SEM), and an asterisk (*) indicates a statistically significant change in expression in the Cd-treated group versus the saline control ( N = 6 per group, unpaired t -test, p ≤ 0.05).
    Taqman Advanced Mirna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    taqman advanced mirna cdna synthesis kit - by Bioz Stars, 2020-08
    99/100 stars
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    mRNA expression of N-extended AChE, AChE-S, and AChE-R isoforms following treatment of Y79 cells with glucose for 1 h. Y79 cells were pre-treated for 16–24 h in starvation medium (1% FBS and 1 mg/ml of glucose) and then with 3.5 mg/ml glucose for different time intervals. Total RNA was extracted and cDNA was prepared for real-time PCR procedure as described under Materials and Methods. Results are presented as fold of control cells cultured in starvation media. Values are means ± SEM, ( N = 4). * p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Acetylcholinesterase (AChE) is an important link in the apoptotic pathway induced by hyperglycemia in Y79 retinoblastoma cell line

    doi: 10.3389/fnmol.2012.00069

    Figure Lengend Snippet: mRNA expression of N-extended AChE, AChE-S, and AChE-R isoforms following treatment of Y79 cells with glucose for 1 h. Y79 cells were pre-treated for 16–24 h in starvation medium (1% FBS and 1 mg/ml of glucose) and then with 3.5 mg/ml glucose for different time intervals. Total RNA was extracted and cDNA was prepared for real-time PCR procedure as described under Materials and Methods. Results are presented as fold of control cells cultured in starvation media. Values are means ± SEM, ( N = 4). * p

    Article Snippet: RNA samples (0.2 μg) were used for cDNA synthesis prepared by the Verso™ cDNA Kit (Thermo SCIENTIFIC, UK).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture

    Differentiated adipocytes and fat depots express CXCR2 . (A) 3T3‐L1 cells were differentiated into adipocytes and cells stained with oil red‐O (scale bars: 50 μm). (B) mRNA was extracted before and after differentiation, reverse transcribed to cDNA and analyzed for CXCR2 expression relative to the house‐keeping gene 18s. (C) Oil red‐O staining was quantified in undifferentiated and differentiated adipocytes in the absence or presence of two different CXCR2 inhibitors (expressed relative to differentiated cells). (D) Adipocytes differentiated in the absence and presence of a CXCR2 inhibitor 1 were analyzed for PPARγ protein expression relative to GAPDH levels (left panel) and quantified relative to GAPDH using densitometry. Data are plotted as mean (± sem ), where each symbol represents an experimental replicate. Analyzed using an unpaired t test with Welch's correction (B and D) or one‐way ANOVA with TUKEY's multiple comparison test (C). Data are representative of two separate experiments

    Journal: Journal of Leukocyte Biology

    Article Title: The chemokine receptor CXCR2 contributes to murine adipocyte development. The chemokine receptor CXCR2 contributes to murine adipocyte development

    doi: 10.1002/JLB.1A0618-216RR

    Figure Lengend Snippet: Differentiated adipocytes and fat depots express CXCR2 . (A) 3T3‐L1 cells were differentiated into adipocytes and cells stained with oil red‐O (scale bars: 50 μm). (B) mRNA was extracted before and after differentiation, reverse transcribed to cDNA and analyzed for CXCR2 expression relative to the house‐keeping gene 18s. (C) Oil red‐O staining was quantified in undifferentiated and differentiated adipocytes in the absence or presence of two different CXCR2 inhibitors (expressed relative to differentiated cells). (D) Adipocytes differentiated in the absence and presence of a CXCR2 inhibitor 1 were analyzed for PPARγ protein expression relative to GAPDH levels (left panel) and quantified relative to GAPDH using densitometry. Data are plotted as mean (± sem ), where each symbol represents an experimental replicate. Analyzed using an unpaired t test with Welch's correction (B and D) or one‐way ANOVA with TUKEY's multiple comparison test (C). Data are representative of two separate experiments

    Article Snippet: Purified RNA (500 ng) was converted to cDNA using the high capacity RNA to cDNA kit (Thermo Scientific). cDNA was analyzed for CXCR2 expression using the indicated primers (Table ), Q5 High‐Fidelity DNA polymerase and DNTP mix (NewEngland Biolabs, Ipswich, MA, USA) using a standard thermocycler programme.

    Techniques: Staining, Expressing

    Real-time PCR validation of Cd-dysregulated miRNAs. TaqMan ® Advanced miRNA assays were used to validate selected Cd-dysregulated miRNAs. ( A ) miR-21-5p; ( B ) miR-34a-5p; ( C ) miR-146b-5p; ( D ) miR-224-5p; ( E ) miR-3084a-3p; ( F ) miR-3084c-3p; ( G ) miR-455-3p; ( H ) miR-423-5p. The comparative CT method was used to determine the fold change (±SEM), and an asterisk (*) indicates a statistically significant change in expression in the Cd-treated group versus the saline control ( N = 6 per group, unpaired t -test, p ≤ 0.05).

    Journal: Toxics

    Article Title: Cadmium Nephrotoxicity Is Associated with Altered MicroRNA Expression in the Rat Renal Cortex

    doi: 10.3390/toxics6010016

    Figure Lengend Snippet: Real-time PCR validation of Cd-dysregulated miRNAs. TaqMan ® Advanced miRNA assays were used to validate selected Cd-dysregulated miRNAs. ( A ) miR-21-5p; ( B ) miR-34a-5p; ( C ) miR-146b-5p; ( D ) miR-224-5p; ( E ) miR-3084a-3p; ( F ) miR-3084c-3p; ( G ) miR-455-3p; ( H ) miR-423-5p. The comparative CT method was used to determine the fold change (±SEM), and an asterisk (*) indicates a statistically significant change in expression in the Cd-treated group versus the saline control ( N = 6 per group, unpaired t -test, p ≤ 0.05).

    Article Snippet: The cDNA template for PCR was prepared using 10 ng of total RNA sample and the TaqMan® Advanced miRNA cDNA Synthesis kit (Thermo Fisher Scientific) following the manufacturer’s recommended protocol.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    circRNA effects on linear transcripts, miRNAs and tumor growth ( A ) Upon efficient transduction of TUSC3 circ104557 in HCT-116 cells (harboring a methylated CpG island), the TUSC3 linear RNA levels did not change: it was not detected in any of the conditions tested. ( B ) TUSC3 circ104557 transduction in DKO cells (harboring an unmethylated CpG island) did not affect the levels of TUSC3 linear RNA. RNA levels were determined using circular or linear specific qRT-PCR primers. The lentiviral transduction of the empty vector (Mock condition) was used as a control. Experiments were performed in technical triplicates. ( C ) Expression of candidate miRNAs putatively targeted by ATRNL1 circ100686 (miR-378a-3p), SAMD4A circ101356 (miR-660-5p and miR-330-3p) and TUSC3 circ104557 (miR-330-3p) was significantly downregulated in DKO cells, evaluated in three biological replicates by real-time quantitative PCR using TaqMan Advanced MicroRNA Assays. Expression levels were normalized using hsa-miR-345-5p, hsa-miR-191-5p and hsa-miR-423-3p advanced Control miRNA Assays. ( D ) Using the same strategy, expression of miR-330-3p, putatively targeted by TUSC3 circ104557, was also assessed in the gain-of-function cellular model. A significant downregulation of miR-330-3p was detected upon TUSC3 circ104557 transduction in HCT116 cells. ( E ) HCT116-Mock and HCT116-TUSC3 circular cells were injected in the left or right flank of 10 mice, respectively. Tumor volume measured over time (left panel) and tumor weight upon sacrifice (right panel) are shown. Tumor growth was significantly reduced upon TUSC3 circular ectopic expression. ns, nonsignificant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001, using Student’s t -test. Error bars show means ± s.d.

    Journal: Oncotarget

    Article Title: Circular RNA CpG island hypermethylation-associated silencing in human cancer

    doi: 10.18632/oncotarget.25673

    Figure Lengend Snippet: circRNA effects on linear transcripts, miRNAs and tumor growth ( A ) Upon efficient transduction of TUSC3 circ104557 in HCT-116 cells (harboring a methylated CpG island), the TUSC3 linear RNA levels did not change: it was not detected in any of the conditions tested. ( B ) TUSC3 circ104557 transduction in DKO cells (harboring an unmethylated CpG island) did not affect the levels of TUSC3 linear RNA. RNA levels were determined using circular or linear specific qRT-PCR primers. The lentiviral transduction of the empty vector (Mock condition) was used as a control. Experiments were performed in technical triplicates. ( C ) Expression of candidate miRNAs putatively targeted by ATRNL1 circ100686 (miR-378a-3p), SAMD4A circ101356 (miR-660-5p and miR-330-3p) and TUSC3 circ104557 (miR-330-3p) was significantly downregulated in DKO cells, evaluated in three biological replicates by real-time quantitative PCR using TaqMan Advanced MicroRNA Assays. Expression levels were normalized using hsa-miR-345-5p, hsa-miR-191-5p and hsa-miR-423-3p advanced Control miRNA Assays. ( D ) Using the same strategy, expression of miR-330-3p, putatively targeted by TUSC3 circ104557, was also assessed in the gain-of-function cellular model. A significant downregulation of miR-330-3p was detected upon TUSC3 circ104557 transduction in HCT116 cells. ( E ) HCT116-Mock and HCT116-TUSC3 circular cells were injected in the left or right flank of 10 mice, respectively. Tumor volume measured over time (left panel) and tumor weight upon sacrifice (right panel) are shown. Tumor growth was significantly reduced upon TUSC3 circular ectopic expression. ns, nonsignificant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001, using Student’s t -test. Error bars show means ± s.d.

    Article Snippet: In brief, TaqMan™ Advanced miRNA cDNA Synthesis Kit (ThermoFisher, Cat. No. ) was used for retrotranscription.

    Techniques: Transduction, Methylation, Quantitative RT-PCR, Plasmid Preparation, Expressing, Real-time Polymerase Chain Reaction, Injection, Mouse Assay