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Meridian Life Science competent escherichia coli cells
Competent Escherichia Coli Cells, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/competent escherichia coli cells/product/Meridian Life Science
Average 91 stars, based on 3 article reviews
Price from $9.99 to $1999.99
competent escherichia coli cells - by Bioz Stars, 2020-08
91/100 stars

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Clone Assay:

Article Title: Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems.
Article Snippet: .. Although numerous techniques for protein expression and production are available the pace of genome sequencing outstrips our ability to analyze the encoded proteins. ..

Ligation:

Article Title: A CRISPR-Cas9 screen identifies essential CTCF anchor sites for estrogen receptor-driven breast cancer cell proliferation
Article Snippet: .. Annealed oligos were diluted at 1:1000 in sterile water, and ligated to plasmid vector lentiCRISPR v2 (gift from Feng Zhang (Addgene plasmid #52961)) using the following parameters: 50 ng BsmBI (Fermentas) digested plasmid, 1 μl diluted oligo duplex, 1× Ligation Buffer (Roche), 5U T4 DNA Ligase (Roche) incubated at RT/30 min. We did five independent ligation reactions per pool and used them to transform highly competent Escherichia coli cells (EletroSHOX - Bioline, BIO-85038) according to manufacturer's protocol. .. In order to assess the complexity of our libraries, we plated 1 μl of cell transformation mixture on LB agar plates containing Ampicillin, incubated them overnight at 37°C, and counted individual bacterial colonies after 16 h. At this point, we estimated that each individual sgRNA is covered > 100×, ensuring that our libraries have high-complexity and are suitable for pooled screening.

Article Title: Ebola Preparedness: Diagnosis Improvement Using Rapid Approaches for Proficiency Testing
Article Snippet: .. The reaction mixture was then incubated at 70°C for 30 min. Two microliters from this reaction was then added into a 10-μl ligation reaction mixture, which consisted of 2 μl of 5× Express Link T4 DNA ligase buffer (Invitrogen), 1 μl of pCRII vector (25 ng/μl), 4 μl of nuclease-free water, and 1 μl of ExpressLink T4 DNA ligase (5 units), followed by incubation at room temperature for 1 h. The ligated product was transformed into chemically competent Escherichia coli cells (α-Select Bronze Efficiency; Bioline) according to the manufacturer's protocol. .. The recombinant plasmid DNA was sequence verified and linearized using the restriction enzyme HindIII or XhoI for T7 RNA polymerase or SP6 RNA polymerase, respectively.

Isolation:

Article Title: Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems.
Article Snippet: .. Although numerous techniques for protein expression and production are available the pace of genome sequencing outstrips our ability to analyze the encoded proteins. ..

Incubation:

Article Title: A CRISPR-Cas9 screen identifies essential CTCF anchor sites for estrogen receptor-driven breast cancer cell proliferation
Article Snippet: .. Annealed oligos were diluted at 1:1000 in sterile water, and ligated to plasmid vector lentiCRISPR v2 (gift from Feng Zhang (Addgene plasmid #52961)) using the following parameters: 50 ng BsmBI (Fermentas) digested plasmid, 1 μl diluted oligo duplex, 1× Ligation Buffer (Roche), 5U T4 DNA Ligase (Roche) incubated at RT/30 min. We did five independent ligation reactions per pool and used them to transform highly competent Escherichia coli cells (EletroSHOX - Bioline, BIO-85038) according to manufacturer's protocol. .. In order to assess the complexity of our libraries, we plated 1 μl of cell transformation mixture on LB agar plates containing Ampicillin, incubated them overnight at 37°C, and counted individual bacterial colonies after 16 h. At this point, we estimated that each individual sgRNA is covered > 100×, ensuring that our libraries have high-complexity and are suitable for pooled screening.

Article Title: Ebola Preparedness: Diagnosis Improvement Using Rapid Approaches for Proficiency Testing
Article Snippet: .. The reaction mixture was then incubated at 70°C for 30 min. Two microliters from this reaction was then added into a 10-μl ligation reaction mixture, which consisted of 2 μl of 5× Express Link T4 DNA ligase buffer (Invitrogen), 1 μl of pCRII vector (25 ng/μl), 4 μl of nuclease-free water, and 1 μl of ExpressLink T4 DNA ligase (5 units), followed by incubation at room temperature for 1 h. The ligated product was transformed into chemically competent Escherichia coli cells (α-Select Bronze Efficiency; Bioline) according to the manufacturer's protocol. .. The recombinant plasmid DNA was sequence verified and linearized using the restriction enzyme HindIII or XhoI for T7 RNA polymerase or SP6 RNA polymerase, respectively.

Transformation Assay:

Article Title: Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems.
Article Snippet: .. Although numerous techniques for protein expression and production are available the pace of genome sequencing outstrips our ability to analyze the encoded proteins. ..

Article Title: Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA
Article Snippet: .. To multiply the T7-VEE-OKS-iMG plasmid, competent E. coli cells (α -select chemically competent cells from Bioline GmbH, Luckenwalde, Germany) were transformed with 100 ng plasmid DNA and cultivated in LB medium supplemented with 50 μ g/ml ampicillin (Sigma-Aldrich Chemie GmbH, Steinheim, Germany). .. The isolation of plasmids was performed using the QIAprep Spin Miniprep Kit (Qiagen).

Article Title: Ebola Preparedness: Diagnosis Improvement Using Rapid Approaches for Proficiency Testing
Article Snippet: .. The reaction mixture was then incubated at 70°C for 30 min. Two microliters from this reaction was then added into a 10-μl ligation reaction mixture, which consisted of 2 μl of 5× Express Link T4 DNA ligase buffer (Invitrogen), 1 μl of pCRII vector (25 ng/μl), 4 μl of nuclease-free water, and 1 μl of ExpressLink T4 DNA ligase (5 units), followed by incubation at room temperature for 1 h. The ligated product was transformed into chemically competent Escherichia coli cells (α-Select Bronze Efficiency; Bioline) according to the manufacturer's protocol. .. The recombinant plasmid DNA was sequence verified and linearized using the restriction enzyme HindIII or XhoI for T7 RNA polymerase or SP6 RNA polymerase, respectively.

Article Title: Characterization of microsatellite loci in Tilia platyphyllos (Malvaceae) and cross-amplification in related species 1
Article Snippet: .. The plasmids were transformed into competent E. coli cells (Bioline, London, United Kingdom). .. Recombinant colonies were selected by blue/white screening, and M13 PCR amplification was used to estimate the size of the inserts.

Plasmid Preparation:

Article Title: Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems.
Article Snippet: .. Although numerous techniques for protein expression and production are available the pace of genome sequencing outstrips our ability to analyze the encoded proteins. ..

Article Title: A CRISPR-Cas9 screen identifies essential CTCF anchor sites for estrogen receptor-driven breast cancer cell proliferation
Article Snippet: .. Annealed oligos were diluted at 1:1000 in sterile water, and ligated to plasmid vector lentiCRISPR v2 (gift from Feng Zhang (Addgene plasmid #52961)) using the following parameters: 50 ng BsmBI (Fermentas) digested plasmid, 1 μl diluted oligo duplex, 1× Ligation Buffer (Roche), 5U T4 DNA Ligase (Roche) incubated at RT/30 min. We did five independent ligation reactions per pool and used them to transform highly competent Escherichia coli cells (EletroSHOX - Bioline, BIO-85038) according to manufacturer's protocol. .. In order to assess the complexity of our libraries, we plated 1 μl of cell transformation mixture on LB agar plates containing Ampicillin, incubated them overnight at 37°C, and counted individual bacterial colonies after 16 h. At this point, we estimated that each individual sgRNA is covered > 100×, ensuring that our libraries have high-complexity and are suitable for pooled screening.

Article Title: Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA
Article Snippet: .. To multiply the T7-VEE-OKS-iMG plasmid, competent E. coli cells (α -select chemically competent cells from Bioline GmbH, Luckenwalde, Germany) were transformed with 100 ng plasmid DNA and cultivated in LB medium supplemented with 50 μ g/ml ampicillin (Sigma-Aldrich Chemie GmbH, Steinheim, Germany). .. The isolation of plasmids was performed using the QIAprep Spin Miniprep Kit (Qiagen).

Article Title: Ebola Preparedness: Diagnosis Improvement Using Rapid Approaches for Proficiency Testing
Article Snippet: .. The reaction mixture was then incubated at 70°C for 30 min. Two microliters from this reaction was then added into a 10-μl ligation reaction mixture, which consisted of 2 μl of 5× Express Link T4 DNA ligase buffer (Invitrogen), 1 μl of pCRII vector (25 ng/μl), 4 μl of nuclease-free water, and 1 μl of ExpressLink T4 DNA ligase (5 units), followed by incubation at room temperature for 1 h. The ligated product was transformed into chemically competent Escherichia coli cells (α-Select Bronze Efficiency; Bioline) according to the manufacturer's protocol. .. The recombinant plasmid DNA was sequence verified and linearized using the restriction enzyme HindIII or XhoI for T7 RNA polymerase or SP6 RNA polymerase, respectively.

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    Meridian Life Science e coli strain ms427pehag
    EhaG promotes autoaggregation and biofilm formation in E. coli . (A) Settling profile of liquid suspensions of E. coli strains MS427pBAD (vector control) and <t>MS427pEhaG.</t> Suspensions were prepared from overnight LB cultures supplemented with 0.2% arabinose
    E Coli Strain Ms427pehag, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain ms427pehag/product/Meridian Life Science
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli strain ms427pehag - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    89
    Meridian Life Science coli o157 h7 antibodies
    Cyclic voltammograms of immuno-c/sNP-cell solutions: ( a ) B. cereus cell concentrations ranging from 4 to 3.9 × 10 2 CFU/mL; and ( b ) E. coli <t>O157:H7</t> cell concentrations ranging from 6 CFU/mL to 5.9 × 10 4 CFU/mL.
    Coli O157 H7 Antibodies, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli o157 h7 antibodies/product/Meridian Life Science
    Average 89 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    coli o157 h7 antibodies - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    Image Search Results


    EhaG promotes autoaggregation and biofilm formation in E. coli . (A) Settling profile of liquid suspensions of E. coli strains MS427pBAD (vector control) and MS427pEhaG. Suspensions were prepared from overnight LB cultures supplemented with 0.2% arabinose

    Journal: Applied and Environmental Microbiology

    Article Title: Molecular Characterization of the EhaG and UpaG Trimeric Autotransporter Proteins from Pathogenic Escherichia coli

    doi: 10.1128/AEM.06680-11

    Figure Lengend Snippet: EhaG promotes autoaggregation and biofilm formation in E. coli . (A) Settling profile of liquid suspensions of E. coli strains MS427pBAD (vector control) and MS427pEhaG. Suspensions were prepared from overnight LB cultures supplemented with 0.2% arabinose

    Article Snippet: Wells were washed twice with TBS (137 mM NaCl, 10 mM Tris, pH 7.4) and then blocked with TBS-2% milk for 1 h. After being washed with TBS, 200 μl of washed and standardized (OD600 of 0.1) cultures of E. coli strain MS427pEhaG, MS427pUpaG, or MS427pBAD was added to 12 replicate wells per ECM component and the plates were incubated at 37°C for 2 h. After being washed to remove nonadherent bacteria, adherent cells were fixed with 4% paraformaldehyde (PFA), washed, incubated for 1 h with anti- E. coli serum (Meridian Life Sciences Inc.; catalogue no. B65001R) diluted 1:500 in 0.05% TBS-Tween–0.2% skim milk, washed, and incubated for 1 h with a secondary anti-rabbit antibody conjugated with horseradish peroxidase (diluted 1:1,000) (Sigma-Aldrich; catalogue no. A6154).

    Techniques: Plasmid Preparation

    EhaG and UpaG mediate E. coli adherence to ECM proteins. ELISA-based assay demonstrating binding of E. coli MS427pEhaG (black bars), E. coli MS427pUpaG (gray bars), and E. coli MS427pBAD (white bars) to collagen (I to V), fibronectin, fibrinogen, and

    Journal: Applied and Environmental Microbiology

    Article Title: Molecular Characterization of the EhaG and UpaG Trimeric Autotransporter Proteins from Pathogenic Escherichia coli

    doi: 10.1128/AEM.06680-11

    Figure Lengend Snippet: EhaG and UpaG mediate E. coli adherence to ECM proteins. ELISA-based assay demonstrating binding of E. coli MS427pEhaG (black bars), E. coli MS427pUpaG (gray bars), and E. coli MS427pBAD (white bars) to collagen (I to V), fibronectin, fibrinogen, and

    Article Snippet: Wells were washed twice with TBS (137 mM NaCl, 10 mM Tris, pH 7.4) and then blocked with TBS-2% milk for 1 h. After being washed with TBS, 200 μl of washed and standardized (OD600 of 0.1) cultures of E. coli strain MS427pEhaG, MS427pUpaG, or MS427pBAD was added to 12 replicate wells per ECM component and the plates were incubated at 37°C for 2 h. After being washed to remove nonadherent bacteria, adherent cells were fixed with 4% paraformaldehyde (PFA), washed, incubated for 1 h with anti- E. coli serum (Meridian Life Sciences Inc.; catalogue no. B65001R) diluted 1:500 in 0.05% TBS-Tween–0.2% skim milk, washed, and incubated for 1 h with a secondary anti-rabbit antibody conjugated with horseradish peroxidase (diluted 1:1,000) (Sigma-Aldrich; catalogue no. A6154).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    Cyclic voltammograms of immuno-c/sNP-cell solutions: ( a ) B. cereus cell concentrations ranging from 4 to 3.9 × 10 2 CFU/mL; and ( b ) E. coli O157:H7 cell concentrations ranging from 6 CFU/mL to 5.9 × 10 4 CFU/mL.

    Journal: Biosensors

    Article Title: Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

    doi: 10.3390/bios2010015

    Figure Lengend Snippet: Cyclic voltammograms of immuno-c/sNP-cell solutions: ( a ) B. cereus cell concentrations ranging from 4 to 3.9 × 10 2 CFU/mL; and ( b ) E. coli O157:H7 cell concentrations ranging from 6 CFU/mL to 5.9 × 10 4 CFU/mL.

    Article Snippet: Polyclonal anti-Bacillus antibodies and monoclonal anti-E. coli O157:H7 antibodies were obtained from Meridian Life Science, Inc. (Saco, ME, USA).

    Techniques:

    Mean charge transfer values obtained in cyclic voltammetry of immuno-c/sNP-cell solutions: ( a ) B. cereus cell concentrations ranging from 4 to 3.9 × 10 2 CFU/mL ( n = 3); ( b ) E. coli O157:H7 cell concentrations ranging from 6 CFU/mL to 5.9 × 10 4 CFU/mL ( n = 3); and ( c ) B. cereus and E. coli results displayed together ( n = 6). Error bars represent ± one standard deviation.

    Journal: Biosensors

    Article Title: Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

    doi: 10.3390/bios2010015

    Figure Lengend Snippet: Mean charge transfer values obtained in cyclic voltammetry of immuno-c/sNP-cell solutions: ( a ) B. cereus cell concentrations ranging from 4 to 3.9 × 10 2 CFU/mL ( n = 3); ( b ) E. coli O157:H7 cell concentrations ranging from 6 CFU/mL to 5.9 × 10 4 CFU/mL ( n = 3); and ( c ) B. cereus and E. coli results displayed together ( n = 6). Error bars represent ± one standard deviation.

    Article Snippet: Polyclonal anti-Bacillus antibodies and monoclonal anti-E. coli O157:H7 antibodies were obtained from Meridian Life Science, Inc. (Saco, ME, USA).

    Techniques: Standard Deviation

    Immunomagnetic separation from sample matrix, magnetic alignment on SPCE surface, and electrochemical detection of B. cereus and E. coli O157:H7 cells.

    Journal: Biosensors

    Article Title: Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

    doi: 10.3390/bios2010015

    Figure Lengend Snippet: Immunomagnetic separation from sample matrix, magnetic alignment on SPCE surface, and electrochemical detection of B. cereus and E. coli O157:H7 cells.

    Article Snippet: Polyclonal anti-Bacillus antibodies and monoclonal anti-E. coli O157:H7 antibodies were obtained from Meridian Life Science, Inc. (Saco, ME, USA).

    Techniques: Immunomagnetic Separation

    Cyclic voltammograms ( a ) and charge transfer values; ( b ) for electrochemical tests performed on pure B. cereus and E. coli O157:H7 cells, suspended at various concentrations in 0.1 M HCl solution, in the absence of c/sNPs.

    Journal: Biosensors

    Article Title: Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

    doi: 10.3390/bios2010015

    Figure Lengend Snippet: Cyclic voltammograms ( a ) and charge transfer values; ( b ) for electrochemical tests performed on pure B. cereus and E. coli O157:H7 cells, suspended at various concentrations in 0.1 M HCl solution, in the absence of c/sNPs.

    Article Snippet: Polyclonal anti-Bacillus antibodies and monoclonal anti-E. coli O157:H7 antibodies were obtained from Meridian Life Science, Inc. (Saco, ME, USA).

    Techniques: