combretastatin a4  (Millipore)


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  • 94
    Name:
    Combretastatin A4
    Description:

    Catalog Number:
    c7744
    Price:
    None
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    Structured Review

    Millipore combretastatin a4
    Combretastatin A4

    https://www.bioz.com/result/combretastatin a4/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    combretastatin a4 - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "End-binding proteins sensitize microtubules to the action of microtubule-targeting agents"

    Article Title: End-binding proteins sensitize microtubules to the action of microtubule-targeting agents

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1300395110

    Effects of MT-destabilizing MTAs on MT dynamics. ( A ) Representative kymographs illustrating the dynamics of rhodamine-labeled MTs grown in the absence of GFP-EB3 ( Left ) or GFP-EB3 labeled MTs ( Right ) in the presence of MT-destabilizing MTAs. The positions of MT seeds are indicated by black lines above the kymographs. ( B ) MTA concentrations sufficient to inhibit MT outgrowth from the seed, so that no MT elongation exceeding 0.5 μm was observed within a 10-min period. ( C–H ) Length of MT growth episodes, MT growth rate, and catastrophe frequency at increasing concentrations of the indicated MTAs. Error bars represent SEM. Abbreviations: CA4, combretastatin A4; Col, colchicine; Dola, dolastatin 15; Podo, podophyllotoxin; VB, vinblastine; VC, vincristine; VR, vinorelbine.
    Figure Legend Snippet: Effects of MT-destabilizing MTAs on MT dynamics. ( A ) Representative kymographs illustrating the dynamics of rhodamine-labeled MTs grown in the absence of GFP-EB3 ( Left ) or GFP-EB3 labeled MTs ( Right ) in the presence of MT-destabilizing MTAs. The positions of MT seeds are indicated by black lines above the kymographs. ( B ) MTA concentrations sufficient to inhibit MT outgrowth from the seed, so that no MT elongation exceeding 0.5 μm was observed within a 10-min period. ( C–H ) Length of MT growth episodes, MT growth rate, and catastrophe frequency at increasing concentrations of the indicated MTAs. Error bars represent SEM. Abbreviations: CA4, combretastatin A4; Col, colchicine; Dola, dolastatin 15; Podo, podophyllotoxin; VB, vinblastine; VC, vincristine; VR, vinorelbine.

    Techniques Used: Labeling

    2) Product Images from "Vinblastine rapidly induces NOXA and acutely sensitizes primary chronic lymphocytic leukemia cells to ABT-737"

    Article Title: Vinblastine rapidly induces NOXA and acutely sensitizes primary chronic lymphocytic leukemia cells to ABT-737

    Journal: Molecular cancer therapeutics

    doi: 10.1158/1535-7163.MCT-12-1197

    Other microtubule disrupting drugs induce Noxa and sensitize CLL cells to ABT-737. Freshly-isolated lymphocytes from CLL patients were incubated with vinblastine, vincristine, vinorelbine or combretastatin A4 alone (A) or in combination with ABT-737 (B)
    Figure Legend Snippet: Other microtubule disrupting drugs induce Noxa and sensitize CLL cells to ABT-737. Freshly-isolated lymphocytes from CLL patients were incubated with vinblastine, vincristine, vinorelbine or combretastatin A4 alone (A) or in combination with ABT-737 (B)

    Techniques Used: Isolation, Incubation

    Related Articles

    Positive Control:

    Article Title: Identification of novel 1-indolyl acetate-5-nitroimidazole derivatives of combretastatin A-4 as potential tubulin polymerization inhibitors.
    Article Snippet: .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells. .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells.

    Article Title: Molecular, Biological and Structural Features of VL CDR-1 Rb44 Peptide, Which Targets the Microtubule Network in Melanoma Cells
    Article Snippet: .. The microtubule depolymerizing CA4 (combretastatin A4, Sigma-Adrich, St. Louis, MO) was used at 75 μM as positive control of G2/M cell cycle arrest. .. Transmission Electron Microscopy B16F10-Nex2 cells (1 × 106 ) were seeded in 6-well plates.

    Generated:

    Article Title: Human iPSC-Derived Endothelial Cell Sprouting Assay in Synthetic Hydrogel Arrays
    Article Snippet: .. Inhibitor stock solutions were generated for SU5416 (20 mM; Sigma), temsirolimus (20 mM; Sigma), sunitinib malate (20 mM; Sigma), SB-3CT (16 mM; Sigma), nilotinib (20 mM; Selleck Chemicals), vatalanib (20 mM; Selleck Chemicals), combretastatin A4 (20 mM; Sigma), withaferin A (20 mM; Sigma), and (+-)-thalidomide (100 mM; Sigma). .. Compound plates containing the ToxCast Phase I and II chemical library[ ] dissolved at 6–20 mM in DMSO were provided via Materials Transfer Agreement by the US Environmental Protection Agency (Research Triangle Park, NC).

    Cell Culture:

    Article Title: Identification of novel 1-indolyl acetate-5-nitroimidazole derivatives of combretastatin A-4 as potential tubulin polymerization inhibitors.
    Article Snippet: .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells. .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells.

    Concentration Assay:

    Article Title: Identification of novel 1-indolyl acetate-5-nitroimidazole derivatives of combretastatin A-4 as potential tubulin polymerization inhibitors.
    Article Snippet: .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells. .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells.

    Incubation:

    Article Title: Identification of novel 1-indolyl acetate-5-nitroimidazole derivatives of combretastatin A-4 as potential tubulin polymerization inhibitors.
    Article Snippet: .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells. .. Microtubules are essential for the mitotic division of cells and have become an attractive target for anti-tumour drugs due to the increased incidence of cancer and significant mitosis rate of tumour cells.

    Activity Assay:

    Article Title: Structurally simplified biphenyl combretastatin A4 derivatives retain in vitro anti-cancer activity dependent on mitotic arrest
    Article Snippet: .. Reagents Two biphenyl analogues (MP5-F9 and MP5-G9) of CA4 were prepared as previously reported [ ] and their biological activity was compared to CA4 (Sigma-Aldrich Canada, Cat. No. C7744, Mississauga, ON, Canada). .. Reversine (Sigma-Aldrich Canada, Cat. No. R3904, Mississauga, ON, Canada) and RO-3306 (Sigma-Aldrich Canada Cat. No. SML0569, Mississauga, ON, Canada), small molecule inhibitors known to prevent mitotic arrest, were used to test the causal connection between mitotic arrest and apoptosis.

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    Millipore reversine
    Additional characterization of ‘loop swap’ mutants. ( A ) Representative images of HeLa cells transfected with GFP-BubR1 wt treated as in Figure 3E in the presence (+) or absence (−) of the Mps1 inhibitor <t>Reversine</t> as control for the experiment shown in Figure 3G . The corresponding quantification is shown in Figure 3H . Scale bar: 10 µm. ( B ) Quantification of Bub1 kinetochore levels in cells treated as in Figure 3E . The graph shows mean intensity from three independent experiments. Error bars represent SEM. Values for Bub1 in BubR1 wt expressing cells are set to 1. ( C ) Quantification of the Western Blot in Figure 3J . The amounts of co-precipitating MCC and APC/C components were normalized to the amount of GFP-BubR1 bait present in the IP. Values for GFP-BubR1 wt are set to 1. The graphs show mean intensity of two independent experiments. Error bars represent SEM. ( D ) Quantification of the Western Blot in Figure 3K . The amounts of co-precipitating proteins were normalized to the amounts present in GFP-BubR1 wt expressing cells. The graphs show mean intensity of two independent experiments. Error bars represent SEM. DOI: http://dx.doi.org/10.7554/eLife.05269.008
    Reversine, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversine/product/Millipore
    Average 98 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    reversine - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    94
    Millipore combretastatin a 4
    Additional characterization of ‘loop swap’ mutants. ( A ) Representative images of HeLa cells transfected with GFP-BubR1 wt treated as in Figure 3E in the presence (+) or absence (−) of the Mps1 inhibitor <t>Reversine</t> as control for the experiment shown in Figure 3G . The corresponding quantification is shown in Figure 3H . Scale bar: 10 µm. ( B ) Quantification of Bub1 kinetochore levels in cells treated as in Figure 3E . The graph shows mean intensity from three independent experiments. Error bars represent SEM. Values for Bub1 in BubR1 wt expressing cells are set to 1. ( C ) Quantification of the Western Blot in Figure 3J . The amounts of co-precipitating MCC and APC/C components were normalized to the amount of GFP-BubR1 bait present in the IP. Values for GFP-BubR1 wt are set to 1. The graphs show mean intensity of two independent experiments. Error bars represent SEM. ( D ) Quantification of the Western Blot in Figure 3K . The amounts of co-precipitating proteins were normalized to the amounts present in GFP-BubR1 wt expressing cells. The graphs show mean intensity of two independent experiments. Error bars represent SEM. DOI: http://dx.doi.org/10.7554/eLife.05269.008
    Combretastatin A 4, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/combretastatin a 4/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    combretastatin a 4 - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Additional characterization of ‘loop swap’ mutants. ( A ) Representative images of HeLa cells transfected with GFP-BubR1 wt treated as in Figure 3E in the presence (+) or absence (−) of the Mps1 inhibitor Reversine as control for the experiment shown in Figure 3G . The corresponding quantification is shown in Figure 3H . Scale bar: 10 µm. ( B ) Quantification of Bub1 kinetochore levels in cells treated as in Figure 3E . The graph shows mean intensity from three independent experiments. Error bars represent SEM. Values for Bub1 in BubR1 wt expressing cells are set to 1. ( C ) Quantification of the Western Blot in Figure 3J . The amounts of co-precipitating MCC and APC/C components were normalized to the amount of GFP-BubR1 bait present in the IP. Values for GFP-BubR1 wt are set to 1. The graphs show mean intensity of two independent experiments. Error bars represent SEM. ( D ) Quantification of the Western Blot in Figure 3K . The amounts of co-precipitating proteins were normalized to the amounts present in GFP-BubR1 wt expressing cells. The graphs show mean intensity of two independent experiments. Error bars represent SEM. DOI: http://dx.doi.org/10.7554/eLife.05269.008

    Journal: eLife

    Article Title: A molecular basis for the differential roles of Bub1 and BubR1 in the spindle assembly checkpoint

    doi: 10.7554/eLife.05269

    Figure Lengend Snippet: Additional characterization of ‘loop swap’ mutants. ( A ) Representative images of HeLa cells transfected with GFP-BubR1 wt treated as in Figure 3E in the presence (+) or absence (−) of the Mps1 inhibitor Reversine as control for the experiment shown in Figure 3G . The corresponding quantification is shown in Figure 3H . Scale bar: 10 µm. ( B ) Quantification of Bub1 kinetochore levels in cells treated as in Figure 3E . The graph shows mean intensity from three independent experiments. Error bars represent SEM. Values for Bub1 in BubR1 wt expressing cells are set to 1. ( C ) Quantification of the Western Blot in Figure 3J . The amounts of co-precipitating MCC and APC/C components were normalized to the amount of GFP-BubR1 bait present in the IP. Values for GFP-BubR1 wt are set to 1. The graphs show mean intensity of two independent experiments. Error bars represent SEM. ( D ) Quantification of the Western Blot in Figure 3K . The amounts of co-precipitating proteins were normalized to the amounts present in GFP-BubR1 wt expressing cells. The graphs show mean intensity of two independent experiments. Error bars represent SEM. DOI: http://dx.doi.org/10.7554/eLife.05269.008

    Article Snippet: Reversine (Calbiochem, part of EMD Biosciences, Darmstadt, Germany) was used at 0.5 µM.

    Techniques: Transfection, Expressing, Western Blot

    C-MAD2 is incorporated into the MCC in the presence of reversine. A , outline of the cell lysate preparation protocol for the experiments shown in B . Cells transfected with GST-tagged MAD2 L13A (C-MAD2) or MAD2 ΔC10 (O-MAD2) or untransfected cells

    Journal: The Journal of Biological Chemistry

    Article Title: Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex *

    doi: 10.1074/jbc.M113.522375

    Figure Lengend Snippet: C-MAD2 is incorporated into the MCC in the presence of reversine. A , outline of the cell lysate preparation protocol for the experiments shown in B . Cells transfected with GST-tagged MAD2 L13A (C-MAD2) or MAD2 ΔC10 (O-MAD2) or untransfected cells

    Article Snippet: Reversine (Calbiochem) was used at 500 n m ( ).

    Techniques: Transfection

    C-MAD2 does not stay at unattached kinetochores in the presence of reversine. A , HeLa-mRFP-H2A cells transfected with GFP-MAD2 L13A were treated with MG132 for 1 h, followed by nocodazole ( Noc ) addition for 1 h. Reversine was then added, and cells were

    Journal: The Journal of Biological Chemistry

    Article Title: Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex *

    doi: 10.1074/jbc.M113.522375

    Figure Lengend Snippet: C-MAD2 does not stay at unattached kinetochores in the presence of reversine. A , HeLa-mRFP-H2A cells transfected with GFP-MAD2 L13A were treated with MG132 for 1 h, followed by nocodazole ( Noc ) addition for 1 h. Reversine was then added, and cells were

    Article Snippet: Reversine (Calbiochem) was used at 500 n m ( ).

    Techniques: Transfection

    C-MAD2 expression in reversine-treated cells rescues MCC assembly and checkpoint responses. A and B , HeLa cells expressing GFP-tagged MAD2 L13A (C-MAD2) ( A ) or MAD2 ΔC10 (O-MAD2) ( B ) were arrested in nocodazole. Live cell imaging was started ∼3

    Journal: The Journal of Biological Chemistry

    Article Title: Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex *

    doi: 10.1074/jbc.M113.522375

    Figure Lengend Snippet: C-MAD2 expression in reversine-treated cells rescues MCC assembly and checkpoint responses. A and B , HeLa cells expressing GFP-tagged MAD2 L13A (C-MAD2) ( A ) or MAD2 ΔC10 (O-MAD2) ( B ) were arrested in nocodazole. Live cell imaging was started ∼3

    Article Snippet: Reversine (Calbiochem) was used at 500 n m ( ).

    Techniques: Expressing, Live Cell Imaging

    Reversine compromises MCC-APC/C interaction and APC/C inhibition. A , cells were synchronized and treated as in A . Cell lysates were then subjected to CDC27 immunoprecipitation ( lanes 2–7 ). CDC27 IPs as a source of APC/Cs were incubated in

    Journal: The Journal of Biological Chemistry

    Article Title: Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex *

    doi: 10.1074/jbc.M113.522375

    Figure Lengend Snippet: Reversine compromises MCC-APC/C interaction and APC/C inhibition. A , cells were synchronized and treated as in A . Cell lysates were then subjected to CDC27 immunoprecipitation ( lanes 2–7 ). CDC27 IPs as a source of APC/Cs were incubated in

    Article Snippet: Reversine (Calbiochem) was used at 500 n m ( ).

    Techniques: Inhibition, Immunoprecipitation, Incubation

    BUBR1 phosphorylation is not required for MCC assembly or stability. A , cells were synchronized and treated as in A using reversine or other small molecule inhibitors. Cell lysates were then analyzed for BUBR1. Noc , nocodazole. B , cell lysates from

    Journal: The Journal of Biological Chemistry

    Article Title: Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex *

    doi: 10.1074/jbc.M113.522375

    Figure Lengend Snippet: BUBR1 phosphorylation is not required for MCC assembly or stability. A , cells were synchronized and treated as in A using reversine or other small molecule inhibitors. Cell lysates were then analyzed for BUBR1. Noc , nocodazole. B , cell lysates from

    Article Snippet: Reversine (Calbiochem) was used at 500 n m ( ).

    Techniques: