column dnase digestion  (Qiagen)


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    Structured Review

    Qiagen column dnase digestion
    Column Dnase Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/column dnase digestion/product/Qiagen
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    column dnase digestion - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    DNA Synthesis:

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms
    Article Snippet: Expression of mcr-1 was assayed by a two-step qRT-PCR using primers mcr-1 -qF, mcr-1 -qR and mcr-1 probe, with Precision 2× qPCR Mastermix (PrimerDesign, UK) following manufacturers’ protocol. .. Total RNA was extracted from bacteria using the RNeasy Plus kit with on column DNase digestion (Qiagen, Germany), followed by complementary DNA synthesis with DNA-integrated genomic DNA (gDNA) removal using QuantiTect Reverse Transcription kit (Qiagen, Germany), according to manufacturers’ protocol. .. The absence of carry-over gDNA was verified for every experiment by comparative qPCR (or standard PCR) in the absence of reverse transcriptase. rpoB expression level was used as internal control using primers rpoB -qF, rpoB -qR and rpoB probe.

    Synthesized:

    Article Title: MYB induces the expression of the oncogenic corepressor SKI in acute myeloid leukemia
    Article Snippet: Cells were selected and maintained in the presence of 1 μg/ml puromycin (pINDUCER) or 600 μg/ml hygromycin (pMSCV). .. Total RNA was isolated using the RNeasy Mini Kit with on column DNase digestion (QIAGEN, Hilden, Germany). cDNA was synthesized via QuantiTect Reverse Transcription Kit (QIAGEN). .. RT-qPCR were carried out via the QuantiTect SYBR Green PCR Kit (QIAGEN).

    Quantitative RT-PCR:

    Article Title: Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells
    Article Snippet: RNA was isolated using RNeasy spin columns including on column DNase digestion (Qiagen, Germantown, MD). .. RNA was isolated using RNeasy spin columns including on column DNase digestion (Qiagen, Germantown, MD).

    Article Title: Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity
    Article Snippet: 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany). .. 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany).

    Article Title: The Effect of ASIC3 Knockout on Corticostriatal Circuit and Mouse Self-grooming Behavior
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR). .. RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol.

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms
    Article Snippet: Expression of mcr-1 was assayed by a two-step qRT-PCR using primers mcr-1 -qF, mcr-1 -qR and mcr-1 probe, with Precision 2× qPCR Mastermix (PrimerDesign, UK) following manufacturers’ protocol. .. Total RNA was extracted from bacteria using the RNeasy Plus kit with on column DNase digestion (Qiagen, Germany), followed by complementary DNA synthesis with DNA-integrated genomic DNA (gDNA) removal using QuantiTect Reverse Transcription kit (Qiagen, Germany), according to manufacturers’ protocol.

    Article Title: Targeting lysyl oxidase reduces peritoneal fibrosis
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and quantitative real-time PCR (qRT–PCR) ... RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions.

    Article Title: MYB induces the expression of the oncogenic corepressor SKI in acute myeloid leukemia
    Article Snippet: Paragraph title: RNA isolation and reverse transcription quantitative PCR (RT-qPCR) ... Total RNA was isolated using the RNeasy Mini Kit with on column DNase digestion (QIAGEN, Hilden, Germany). cDNA was synthesized via QuantiTect Reverse Transcription Kit (QIAGEN).

    SYBR Green Assay:

    Article Title: Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Article Snippet: Total RNA of monolayer cells was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen, Hilden Germany) according to instructions of manufacturers. .. After complete homogenization with proteinase k at 55°C (Qiagen), total RNA was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen).

    Article Title: The Effect of ASIC3 Knockout on Corticostriatal Circuit and Mouse Self-grooming Behavior
    Article Snippet: RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol. .. RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol.

    Microarray:

    Article Title: XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway
    Article Snippet: Paragraph title: Gene Expression Microarray Analysis ... Total RNA was extracted by using RNeasy mini kit with on column DNase digestion (QIAGEN).

    Article Title: Multipotent luminal mammary cancer stem cells model tumor heterogeneity
    Article Snippet: RNAs were prepared using the RNeasy kit with on column DNAse digestion (Qiagen) according to the manufacturer’s protocol. .. To match our MaCSC-derived syngeneic murine breast tumors to their most relevant human breast cancer subtypes based on the global similarities in gene expression pattern, we carried out transcriptome profiling on our murine tumors and then employed a supervised learning approach to assign the subtype labels to each murine tumor.

    Infection:

    Article Title: XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway
    Article Snippet: MDA-MB-231 cells infected with control shRNA or XBP1 shRNA lentiviruses grown in glucose free medium were treated in 0.1% O2 in a hypoxia chamber for 24h. .. Total RNA was extracted by using RNeasy mini kit with on column DNase digestion (QIAGEN).

    Expressing:

    Article Title: XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway
    Article Snippet: Paragraph title: Gene Expression Microarray Analysis ... Total RNA was extracted by using RNeasy mini kit with on column DNase digestion (QIAGEN).

    Article Title: Multipotent luminal mammary cancer stem cells model tumor heterogeneity
    Article Snippet: RNAs were prepared using the RNeasy kit with on column DNAse digestion (Qiagen) according to the manufacturer’s protocol. .. RNA integrity was checked by electropherogram (Agilent Technologies, Santa Clara, CA, USA) and applied to Nimblegen expression arrays (Roche, Pleasanton, CA, USA).

    Article Title: Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells
    Article Snippet: RNA was isolated using RNeasy spin columns including on column DNase digestion (Qiagen, Germantown, MD). .. RNA was either reverse transcribed using the RT2 first strand kit (SA Biosciences, Frederick, MD) and gene expression detected using toll like receptor (TLR) signaling pathway RT2 profiler plates with SYBR green master mix (SA Biosciences), or using Superscript III Supermix (Life Technologies) followed by gene expression assays (Life Technologies).

    Article Title: Epithelial SCAP/INSIG/SREBP Signaling Regulates Multiple Biological Processes during Perinatal Lung Maturation
    Article Snippet: miRNA expression profile was determined by using GeneChip miRNA 3.0 Array ( www.affymetrix.com ). .. Lung samples from ScapΔ/Δ and control littermates at E18.5 (n = 3 per genotype) were used to isolate RNA using miRNeasy kit with on column DNase digestion (Qiagen, Valencia, CA).

    Article Title: Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity
    Article Snippet: 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany). .. 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany).

    Article Title: Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Article Snippet: Furthermore, the gene expression of collagen 2A1 and osteopontin was assessed to examine potential induction of chondrogenic or osteogenic differentiation. .. Total RNA of monolayer cells was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen, Hilden Germany) according to instructions of manufacturers.

    Article Title: The Effect of ASIC3 Knockout on Corticostriatal Circuit and Mouse Self-grooming Behavior
    Article Snippet: Paragraph title: RNA Extraction and Gene Expression Analysis ... RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol.

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms
    Article Snippet: Expression of mcr-1 was assayed by a two-step qRT-PCR using primers mcr-1 -qF, mcr-1 -qR and mcr-1 probe, with Precision 2× qPCR Mastermix (PrimerDesign, UK) following manufacturers’ protocol. .. Total RNA was extracted from bacteria using the RNeasy Plus kit with on column DNase digestion (Qiagen, Germany), followed by complementary DNA synthesis with DNA-integrated genomic DNA (gDNA) removal using QuantiTect Reverse Transcription kit (Qiagen, Germany), according to manufacturers’ protocol.

    Article Title: Targeting lysyl oxidase reduces peritoneal fibrosis
    Article Snippet: RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions. .. RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions.

    Article Title: Sex differences in rat placental development: from pre-implantation to late gestation
    Article Snippet: Paragraph title: Gene expression studies ... Total RNA from labyrinth samples at E13 and E15 were extracted using RNeasy kits with on column DNase digestion (Qiagen).

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: For gene expression analysis, TriZol reagent was added to each well and RNA extraction was performed according to the manufacturer's instructions. .. RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    Ligation:

    Article Title: NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothelium
    Article Snippet: RNA from HAVECs from each condition was purified using TRIzol extraction followed by RNeasy MinElute Cleanup Kit with column DNAse digestion (Qiagen, Valencia, CA). .. 50 ng of RNA was used with the Ovation® RNA-Seq System (7100-08)(NuGEN, San Carlos, CA) and manufacturer's protocol to synthesize and SPIA amplify cDNA, which was then sheared with a covaris S-series.

    Cell Culture:

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: Paragraph title: Cell Culture and Monocyte Differentiation Assay ... RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    Indirect Immunoperoxidase Assay:

    Article Title: Epithelial SCAP/INSIG/SREBP Signaling Regulates Multiple Biological Processes during Perinatal Lung Maturation
    Article Snippet: Lung samples from ScapΔ/Δ and control littermates at E18.5 (n = 3 per genotype) were used to isolate RNA using miRNeasy kit with on column DNase digestion (Qiagen, Valencia, CA). .. Lung samples from ScapΔ/Δ and control littermates at E18.5 (n = 3 per genotype) were used to isolate RNA using miRNeasy kit with on column DNase digestion (Qiagen, Valencia, CA).

    Generated:

    Article Title: A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection
    Article Snippet: Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothelium
    Article Snippet: RNA from HAVECs from each condition was purified using TRIzol extraction followed by RNeasy MinElute Cleanup Kit with column DNAse digestion (Qiagen, Valencia, CA). .. 50 ng of RNA was used with the Ovation® RNA-Seq System (7100-08)(NuGEN, San Carlos, CA) and manufacturer's protocol to synthesize and SPIA amplify cDNA, which was then sheared with a covaris S-series.

    Polymerase Chain Reaction:

    Article Title: Targeting lysyl oxidase reduces peritoneal fibrosis
    Article Snippet: RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions. .. RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions.

    Article Title: Regulation of Hypothalamic Corticotropin-Releasing Hormone Transcription by Elevated Glucocorticoids
    Article Snippet: Total RNA was extracted from primary cultures of hypothalamic neurons using TRIzol reagent, followed by purification using RNeasy minikit reagents and column DNase digestion (QIAGEN) to remove genomic DNA contamination. cDNA was reverse transcribed from 0.3 to 0.75 μg of total RNA, and CRH primary transcript levels (hnRNA) were measured using primer sequences designed to amplify the intron as described previously ( ). .. Total RNA was extracted from primary cultures of hypothalamic neurons using TRIzol reagent, followed by purification using RNeasy minikit reagents and column DNase digestion (QIAGEN) to remove genomic DNA contamination. cDNA was reverse transcribed from 0.3 to 0.75 μg of total RNA, and CRH primary transcript levels (hnRNA) were measured using primer sequences designed to amplify the intron as described previously ( ).

    DNA Sequencing:

    Article Title: NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothelium
    Article Snippet: RNA from HAVECs from each condition was purified using TRIzol extraction followed by RNeasy MinElute Cleanup Kit with column DNAse digestion (Qiagen, Valencia, CA). .. 50 ng of RNA was used with the Ovation® RNA-Seq System (7100-08)(NuGEN, San Carlos, CA) and manufacturer's protocol to synthesize and SPIA amplify cDNA, which was then sheared with a covaris S-series.

    Sequencing:

    Article Title: A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: Response of peanut Arachis hypogaea roots to the presence of beneficial and pathogenic fungi by transcriptome analysis
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: Targeting lysyl oxidase reduces peritoneal fibrosis
    Article Snippet: RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions. .. RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions.

    Article Title: Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNAse digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    RNA Sequencing Assay:

    Article Title: A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection
    Article Snippet: Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothelium
    Article Snippet: Paragraph title: 2.5. RNA-seq and Analysis Pipeline ... RNA from HAVECs from each condition was purified using TRIzol extraction followed by RNeasy MinElute Cleanup Kit with column DNAse digestion (Qiagen, Valencia, CA).

    Multiple Displacement Amplification:

    Article Title: XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway
    Article Snippet: MDA-MB-231 cells infected with control shRNA or XBP1 shRNA lentiviruses grown in glucose free medium were treated in 0.1% O2 in a hypoxia chamber for 24h. .. Total RNA was extracted by using RNeasy mini kit with on column DNase digestion (QIAGEN).

    Isolation:

    Article Title: Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells
    Article Snippet: They were incubated at 37°C with type I or type III IFNs at the concentrations indicated for 24 hours and harvested as described for MDDCs. .. RNA was isolated using RNeasy spin columns including on column DNase digestion (Qiagen, Germantown, MD). .. RNA was either reverse transcribed using the RT2 first strand kit (SA Biosciences, Frederick, MD) and gene expression detected using toll like receptor (TLR) signaling pathway RT2 profiler plates with SYBR green master mix (SA Biosciences), or using Superscript III Supermix (Life Technologies) followed by gene expression assays (Life Technologies).

    Article Title: A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection
    Article Snippet: For RNA sequencing, 12 d old peanut seedlings were treated with MBE02 (106 cells/ml) and root samples of 8-10 different plants/samples were collected after 14 days of treatment, immediately frozen into liquid nitrogen, and stored in −80 °C until further use. .. Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. RNA concentration was then measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA).

    Article Title: Genome wide transcriptome analysis reveals ABA mediated response in Arabidopsis during gold (AuCl−4) treatment
    Article Snippet: Three independent biological replicates were used for each treatment and around 240 seedlings were used in each biological replicate to isolate RNA. .. Total RNA was isolated from frozen root and shoot tissues separately using RNeasy Plant Mini Kit with on column DNase digestion (Qiagen, USA), according to the manufacturer's instructions. .. The integrity of RNA was checked on agarose gel and concentrations were determined using Nanodrop (Wilmington, DE, USA).

    Article Title: Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Article Snippet: GAPDH and ACTB served as housekeeping genes. shows the primer sequences. .. Total RNA of monolayer cells was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen, Hilden Germany) according to instructions of manufacturers. .. Frozen tendon constructs were homogenized with the Tissue Lyser II (Qiagen).

    Article Title: Response of peanut Arachis hypogaea roots to the presence of beneficial and pathogenic fungi by transcriptome analysis
    Article Snippet: To increase the accuracy of detection, three subsamples of peanut roots were bulked into one sample for each RNA seq sample. .. Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. RNA degradation and contamination was detected on 1% agarose gels.

    Article Title: Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection
    Article Snippet: Each sample was repeated twice, and all the four samples were frozen in liquid nitrogen immediately after collection and stored at −80 °C prior to RNA extraction. .. Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNAse digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. RNA degradation and contamination was detected on 1.2% agarose gels.

    Article Title: Regulation of Hypothalamic Corticotropin-Releasing Hormone Transcription by Elevated Glucocorticoids
    Article Snippet: Paragraph title: RNA Isolation and real-time PCR for CRH hnRNA ... Total RNA was extracted from primary cultures of hypothalamic neurons using TRIzol reagent, followed by purification using RNeasy minikit reagents and column DNase digestion (QIAGEN) to remove genomic DNA contamination. cDNA was reverse transcribed from 0.3 to 0.75 μg of total RNA, and CRH primary transcript levels (hnRNA) were measured using primer sequences designed to amplify the intron as described previously ( ).

    Functional Assay:

    Article Title: Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells
    Article Snippet: RNA was isolated using RNeasy spin columns including on column DNase digestion (Qiagen, Germantown, MD). .. All qRT-PCR was performed using the 7900HT or Viia 7 system (both Applied Biosystems) and fold change of gene expression was calculated using the ΔΔCq method.

    Article Title: Epithelial SCAP/INSIG/SREBP Signaling Regulates Multiple Biological Processes during Perinatal Lung Maturation
    Article Snippet: Lung samples from ScapΔ/Δ and control littermates at E18.5 (n = 3 per genotype) were used to isolate RNA using miRNeasy kit with on column DNase digestion (Qiagen, Valencia, CA). .. The miRNAs with P < 0.01 and fold changes > 1.3 was defined as differentially expressed using BRB-ArrayTools Version: 4.2.1 ( http://linus.nci.nih.gov/BRB-ArrayTools.html ). miRNA target prediction and filtering were performed using miRNA Target Filter tool in IPA. miRNA and mRNA targets showing inverse expression patterns in response to Scap deletion at E18.5 were selected for further analysis (i.e. miRNA up, mRNA down OR miRNA down, mRNA up).

    Microscopy:

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: Twenty-four hours later, monocyte cultures were visualized under phase-contrast microscopy or processed for FACS analysis as described. .. RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    Purification:

    Article Title: NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothelium
    Article Snippet: For Western blots, endothelial cells were treated for 48 hours in DMSO or DAPT and then trypsinized, washed with PBS, and re-plated for 96 hours before harvesting in RIPA buffer (Sigma, St. Louis, MO) + 1× Complete Mini protease inhibitors (Roche, Madison, WI). .. RNA from HAVECs from each condition was purified using TRIzol extraction followed by RNeasy MinElute Cleanup Kit with column DNAse digestion (Qiagen, Valencia, CA). .. Then, RNA from the two biological replicates was pooled into one sample per condition prior to library generation.

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: For gene expression analysis, TriZol reagent was added to each well and RNA extraction was performed according to the manufacturer's instructions. .. RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen). .. RNA concentration and purity was determined by Nanodrop and confirmed by agarose gel electrophoresis.

    Article Title: Regulation of Hypothalamic Corticotropin-Releasing Hormone Transcription by Elevated Glucocorticoids
    Article Snippet: The content of the wells was transferred to 1.5-mL microfuge tubes and frozen at −80°C or immediately processed for RNA isolation. .. Total RNA was extracted from primary cultures of hypothalamic neurons using TRIzol reagent, followed by purification using RNeasy minikit reagents and column DNase digestion (QIAGEN) to remove genomic DNA contamination. cDNA was reverse transcribed from 0.3 to 0.75 μg of total RNA, and CRH primary transcript levels (hnRNA) were measured using primer sequences designed to amplify the intron as described previously ( ). .. Power SYBR Green PCR mix (Applied Biosystems) was used for the amplification mixture with each primer at a final concentration of 200 nM and 1.5 μL of cDNA for a total reaction volume of 12.5 μL.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The Effect of ASIC3 Knockout on Corticostriatal Circuit and Mouse Self-grooming Behavior
    Article Snippet: RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol. .. RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol.

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen). .. RNA concentration and purity was determined by Nanodrop and confirmed by agarose gel electrophoresis.

    Labeling:

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: Briefly, a cocktail of biotin-conjugated antibodies against CD3, CD7, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A), as well as anti-Biotin MicroBeads, yields highly pure unlabeled monocytes obtained by depletion of the magnetically labeled cells. .. RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    Plasmid Preparation:

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms
    Article Snippet: Total RNA was extracted from bacteria using the RNeasy Plus kit with on column DNase digestion (Qiagen, Germany), followed by complementary DNA synthesis with DNA-integrated genomic DNA (gDNA) removal using QuantiTect Reverse Transcription kit (Qiagen, Germany), according to manufacturers’ protocol. .. For qPCR determination of mcr-1 copy numbers per cell, 0.1 ng of total gDNA was used as template with primers mcr-1 qF, mcr-1 qR and mcr-1 probe and 16S primers and probe (Supplementary Table ).

    TaqMan Assay:

    Article Title: Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity
    Article Snippet: 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany). .. 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany).

    Real-time Polymerase Chain Reaction:

    Article Title: Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells
    Article Snippet: Paragraph title: Quantitative Real-time PCR ... RNA was isolated using RNeasy spin columns including on column DNase digestion (Qiagen, Germantown, MD).

    Article Title: Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity
    Article Snippet: 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany). .. 2.4 RNA was extracted from snap-frozen tissue samples using the Qiagen RNeasy mini-kit with on column DNase digestion (Qiagen GmBH, Hilden, Germany).

    Article Title: Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Article Snippet: Paragraph title: Real-Time PCR ... Total RNA of monolayer cells was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen, Hilden Germany) according to instructions of manufacturers.

    Article Title: The Effect of ASIC3 Knockout on Corticostriatal Circuit and Mouse Self-grooming Behavior
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR). .. RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol.

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms
    Article Snippet: Paragraph title: Quantitative real-time PCR ... Total RNA was extracted from bacteria using the RNeasy Plus kit with on column DNase digestion (Qiagen, Germany), followed by complementary DNA synthesis with DNA-integrated genomic DNA (gDNA) removal using QuantiTect Reverse Transcription kit (Qiagen, Germany), according to manufacturers’ protocol.

    Article Title: Targeting lysyl oxidase reduces peritoneal fibrosis
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and quantitative real-time PCR (qRT–PCR) ... RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions.

    Article Title: MYB induces the expression of the oncogenic corepressor SKI in acute myeloid leukemia
    Article Snippet: Paragraph title: RNA isolation and reverse transcription quantitative PCR (RT-qPCR) ... Total RNA was isolated using the RNeasy Mini Kit with on column DNase digestion (QIAGEN, Hilden, Germany). cDNA was synthesized via QuantiTect Reverse Transcription Kit (QIAGEN).

    Article Title: Regulation of Hypothalamic Corticotropin-Releasing Hormone Transcription by Elevated Glucocorticoids
    Article Snippet: Paragraph title: RNA Isolation and real-time PCR for CRH hnRNA ... Total RNA was extracted from primary cultures of hypothalamic neurons using TRIzol reagent, followed by purification using RNeasy minikit reagents and column DNase digestion (QIAGEN) to remove genomic DNA contamination. cDNA was reverse transcribed from 0.3 to 0.75 μg of total RNA, and CRH primary transcript levels (hnRNA) were measured using primer sequences designed to amplify the intron as described previously ( ).

    RNA Extraction:

    Article Title: A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Total RNA was isolated by using the RNAeasy plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: Response of peanut Arachis hypogaea roots to the presence of beneficial and pathogenic fungi by transcriptome analysis
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: The Effect of ASIC3 Knockout on Corticostriatal Circuit and Mouse Self-grooming Behavior
    Article Snippet: RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR). .. RNA extraction of brain subregion samples was conducted with RNeasy Mini Kit (Qiagen) with on column DNase digestion (Qiagen), based on the manufacturer’s protocol. .. The RNA concentration and quality were measured by NanoDrop 1000 (Thermo Scientific, Waltham, MA, USA).

    Article Title: Targeting lysyl oxidase reduces peritoneal fibrosis
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and quantitative real-time PCR (qRT–PCR) ... RNA was extracted from mesothelial cell lysates using the RNEasy micro extraction kit, with on column DNAse digestion (Qiagen), following the manufacturer’s instructions.

    Article Title: Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection
    Article Snippet: Paragraph title: RNA extraction, library construction and sequencing ... Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNAse digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: For gene expression analysis, TriZol reagent was added to each well and RNA extraction was performed according to the manufacturer's instructions. .. RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    shRNA:

    Article Title: XBP1 Promotes Triple Negative Breast Cancer By Controlling the HIF1 α Pathway
    Article Snippet: MDA-MB-231 cells infected with control shRNA or XBP1 shRNA lentiviruses grown in glucose free medium were treated in 0.1% O2 in a hypoxia chamber for 24h. .. Total RNA was extracted by using RNeasy mini kit with on column DNase digestion (QIAGEN).

    Homogenization:

    Article Title: Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Article Snippet: Total RNA of monolayer cells was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen, Hilden Germany) according to instructions of manufacturers. .. Frozen tendon constructs were homogenized with the Tissue Lyser II (Qiagen).

    Spectrophotometry:

    Article Title: Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Article Snippet: Total RNA of monolayer cells was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen, Hilden Germany) according to instructions of manufacturers. .. After complete homogenization with proteinase k at 55°C (Qiagen), total RNA was isolated using the RNeasy® Mini Kit with On Column DNase digestion (Qiagen).

    Concentration Assay:

    Article Title: Response of peanut Arachis hypogaea roots to the presence of beneficial and pathogenic fungi by transcriptome analysis
    Article Snippet: Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNase digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection
    Article Snippet: Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNAse digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions. .. Total RNA was isolated from frozen medullar tissue by using the RNA plant mini kit with column DNAse digestion (Qiagen, Hilden, Germany) following the manufacturer’s instructions.

    Differentiation Assay:

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: Paragraph title: Cell Culture and Monocyte Differentiation Assay ... RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    FACS:

    Article Title: TLR9 Differentiates Rapid from Slowly Progressive Forms of Idiopathic Pulmonary Fibrosis
    Article Snippet: Twenty-four hours later, monocyte cultures were visualized under phase-contrast microscopy or processed for FACS analysis as described. .. RNA was purified using the RNAeasy RNA cleanup kit (Quiagen) and subjected to on column DNAase digestion (Quiagen).

    RNA Detection:

    Article Title: Regulation of Hypothalamic Corticotropin-Releasing Hormone Transcription by Elevated Glucocorticoids
    Article Snippet: Total RNA was extracted from primary cultures of hypothalamic neurons using TRIzol reagent, followed by purification using RNeasy minikit reagents and column DNase digestion (QIAGEN) to remove genomic DNA contamination. cDNA was reverse transcribed from 0.3 to 0.75 μg of total RNA, and CRH primary transcript levels (hnRNA) were measured using primer sequences designed to amplify the intron as described previously ( ). .. Levels of hnRNA were normalized to GAPDH mRNA as determined in separate real-time-quantitative (q) PCR reactions.

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    Qiagen column dnase digestion
    Column Dnase Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/column dnase digestion/product/Qiagen
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    Qiagen on column dnase i digestion
    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    On Column Dnase I Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase i digestion/product/Qiagen
    Average 99 stars, based on 235 article reviews
    Price from $9.99 to $1999.99
    on column dnase i digestion - by Bioz Stars, 2020-01
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    Qiagen dnase on column digestion
    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    Dnase On Column Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase on column digestion/product/Qiagen
    Average 93 stars, based on 5 article reviews
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    Image Search Results


    Summary of  in vitro  primer extension and DNase I and KMnO 4  footprinting. ( A ) Sequence of P vpsL  from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4  footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4  cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: In Vitro, Footprinting, Sequencing, Labeling, Binding Assay

    DNase I footprinting of P vpsL  complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: Footprinting, Binding Assay