column dnase digestion  (Qiagen)

 
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    Name:
    RNeasy Plus Mini Kit
    Description:
    For phenol free total RNA extraction from cells tissues with removal of genomic DNA contamination Kit contents Qiagen RNeasy Plus Mini Kit 50 preps 30mg Sample 30 to 50L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format 25 min Time Run Ideal for PCR Northern Dot and Slot Blotting Array Analysis Sequencing For Purification of Up to 100g Total RNA from Cells tissues Using gDNA Eliminator Columns Includes RNeasy Mini Spin Columns gDNA Eliminator Spin Columns Collection Tubes RNase free Water and Buffers Benefits Efficient gDNA removal with unique gDNA Eliminator columns no need for DNase High quality total RNA in minutes using fast and simple extraction protocols Phenol free RNA isolation Ideal for sensitive applications such as real time RT PCR and RNA Seq
    Catalog Number:
    74134
    Price:
    360
    Category:
    RNeasy Plus Micro and Mini Kits
    Buy from Supplier


    Structured Review

    Qiagen column dnase digestion
    RNeasy Plus Mini Kit
    For phenol free total RNA extraction from cells tissues with removal of genomic DNA contamination Kit contents Qiagen RNeasy Plus Mini Kit 50 preps 30mg Sample 30 to 50L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format 25 min Time Run Ideal for PCR Northern Dot and Slot Blotting Array Analysis Sequencing For Purification of Up to 100g Total RNA from Cells tissues Using gDNA Eliminator Columns Includes RNeasy Mini Spin Columns gDNA Eliminator Spin Columns Collection Tubes RNase free Water and Buffers Benefits Efficient gDNA removal with unique gDNA Eliminator columns no need for DNase High quality total RNA in minutes using fast and simple extraction protocols Phenol free RNA isolation Ideal for sensitive applications such as real time RT PCR and RNA Seq
    https://www.bioz.com/result/column dnase digestion/product/Qiagen
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    column dnase digestion - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: The Tumor Suppressive Role of eIF3f and Its Function in Translation Inhibition and rRNA Degradation
    Article Snippet: .. Cells were harvested at 4, 8 and 16 h after transfection and total RNA was isolated using RNeasy kit (QIAGEN). rRNAs (2.0 µg) were separated on an agarose gel and visualized by UV light (B). ..

    Agarose Gel Electrophoresis:

    Article Title: The Tumor Suppressive Role of eIF3f and Its Function in Translation Inhibition and rRNA Degradation
    Article Snippet: .. Cells were harvested at 4, 8 and 16 h after transfection and total RNA was isolated using RNeasy kit (QIAGEN). rRNAs (2.0 µg) were separated on an agarose gel and visualized by UV light (B). ..

    Isolation:

    Article Title: Research Resource: A Genome-Wide Study Identifies Potential New Target Genes for POU1F1
    Article Snippet: .. Total RNA from cells infected with the lentiviral vectors was isolated by using the RNeasy Kit (Qiagen, Hilden, Germany), including on-column DNase I digestion according to the manufacturer's recommendations. .. The quality of isolated RNA was controlled by the Lab-on-Chip-System Bioanalyser 2100 (Agilent Technologies, Inc., Palo Alto, CA).

    Article Title: The Tumor Suppressive Role of eIF3f and Its Function in Translation Inhibition and rRNA Degradation
    Article Snippet: .. Cells were harvested at 4, 8 and 16 h after transfection and total RNA was isolated using RNeasy kit (QIAGEN). rRNAs (2.0 µg) were separated on an agarose gel and visualized by UV light (B). ..

    Article Title: The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma
    Article Snippet: .. Real- time PCR Total RNA was isolated from glioma cells using Qiagen RNeasy kit, 1 μg was used as a template. .. Amplifications were performed in duplicates in 20 μl containing 2× SYBR PCR MasterMix (Applied Biosystems) and a set of primers (human SPP1, NANOG and POU5F1_1_SG/OCT4 primers were from QuantiTect Primer Assays: QT01008798, QT01844808 and QT00210840, respectively, Qiagen).

    Article Title: Mechanisms by which Porphyromonas gingivalis evades innate immunity
    Article Snippet: .. Quantitative RT-PCR BMDCs were cultured in a 24-well plate for 6 h with 108 bacteria/well and RNA was isolated from the cells by RNeasy kit (Qiagen). .. RNA was treated to remove DNA contamination (Invitrogen) and the cDNA was synthesized using random primers, and reverse transcribed using Superscript III (Invitrogen).

    Preserving:

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: .. Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus . .. Their results confirmed that the preservation of copepods in RNAlate r® and the extraction with Qiagen RNeasy Micro Kit were the optimal isolation method for high-quality and quantity of RNA for NGS studies of C . helgolandicus .

    Quantitative RT-PCR:

    Article Title: Mechanisms by which Porphyromonas gingivalis evades innate immunity
    Article Snippet: .. Quantitative RT-PCR BMDCs were cultured in a 24-well plate for 6 h with 108 bacteria/well and RNA was isolated from the cells by RNeasy kit (Qiagen). .. RNA was treated to remove DNA contamination (Invitrogen) and the cDNA was synthesized using random primers, and reverse transcribed using Superscript III (Invitrogen).

    Purification:

    Article Title: Techniques for the Isolation of High-Quality RNA from Cells Encapsulated in Chitosan Hydrogels
    Article Snippet: .. The protocol developed by Wang et al. uses a combination of mechanical disruption, extraction with CTAB buffer and purification with the RNeasy® kit. .. To test the efficacy of this approach with our specific material and cell source, the hydrogels were either (1) cryo-pulverized with a mortar and pestle into a fine powder or (2) finely minced with sharp surgical scissors and then disrupted using an ultrasonic dismembrator (Fisher Scientific Model 100) with three 5-s bursts at a setting of 4 with intervals of cooling on ice in 600 μL of CTAB buffer [2% CTAB, 2% poly(vinylpyrrolidone) (PVP), 1.4 M NaCl, 100 mM Tris-HCl, 20 mM EDTA, and 1% beta-mercaptoethanol in diethylpyrocarbonate (DEPC)-treated water] prewarmed to 65°C.

    Real-time Polymerase Chain Reaction:

    Article Title: The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma
    Article Snippet: .. Real- time PCR Total RNA was isolated from glioma cells using Qiagen RNeasy kit, 1 μg was used as a template. .. Amplifications were performed in duplicates in 20 μl containing 2× SYBR PCR MasterMix (Applied Biosystems) and a set of primers (human SPP1, NANOG and POU5F1_1_SG/OCT4 primers were from QuantiTect Primer Assays: QT01008798, QT01844808 and QT00210840, respectively, Qiagen).

    Infection:

    Article Title: Research Resource: A Genome-Wide Study Identifies Potential New Target Genes for POU1F1
    Article Snippet: .. Total RNA from cells infected with the lentiviral vectors was isolated by using the RNeasy Kit (Qiagen, Hilden, Germany), including on-column DNase I digestion according to the manufacturer's recommendations. .. The quality of isolated RNA was controlled by the Lab-on-Chip-System Bioanalyser 2100 (Agilent Technologies, Inc., Palo Alto, CA).

    Cell Culture:

    Article Title: Mechanisms by which Porphyromonas gingivalis evades innate immunity
    Article Snippet: .. Quantitative RT-PCR BMDCs were cultured in a 24-well plate for 6 h with 108 bacteria/well and RNA was isolated from the cells by RNeasy kit (Qiagen). .. RNA was treated to remove DNA contamination (Invitrogen) and the cDNA was synthesized using random primers, and reverse transcribed using Superscript III (Invitrogen).

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  • 99
    Qiagen on column dnase i digestion
    Summary of in vitro primer extension and <t>DNase</t> I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.
    On Column Dnase I Digestion, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase i digestion/product/Qiagen
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    on column dnase i digestion - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    90
    Qiagen on column dnase i digestion step
    <t>DNase</t> I footprinting assay of EsaR binding sites in the noncoding strand of P dkgA , coding strand of P glpF , and noncoding strand of P lrhA . (A to C) Capillary electrophoresis of FAM-labeled DNA fragments P dkgA (A), P glpF (B), and P lrhA (C) from DNase I
    On Column Dnase I Digestion Step, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/on column dnase i digestion step/product/Qiagen
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    on column dnase i digestion step - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: In Vitro, Footprinting, Sequencing, Labeling, Binding Assay

    DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: Footprinting, Binding Assay

    Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: Summary of in vitro primer extension and DNase I and KMnO 4 footprinting. ( A ) Sequence of P vpsL from −60 to +30. Bold and underlined A with black arrow at +1 and bold G (+3) represent the TSS determined by primer extensions; the −10 element and the −35 region are labeled and boxed in green; sequences in bold and red denote the VpsR binding site. Protection sites from DNase I footprinting and hypersensitive sites are depicted as rectangular boxes and triangles, respectively, either above (non-template) or below (template) the sequences: gray, RNAP with or without c-di-GMP or VpsR; black, RNAP with VpsR and c-di-GMP; red, VpsR with or without c-di-GMP. The open transcription bubble detected using KMnO 4 footprinting is shown as separated ssDNA from position −11 to +2 with sites of KMnO 4 cleavage indicated as purple asterisks. ( B ) Summary of positions of protection and hypersensitive sites on non-template and template strand DNA.

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: In Vitro, Footprinting, Sequencing, Labeling, Binding Assay

    DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Journal: Nucleic Acids Research

    Article Title: VpsR and cyclic di-GMP together drive transcription initiation to activate biofilm formation in Vibrio cholerae

    doi: 10.1093/nar/gky606

    Figure Lengend Snippet: DNase I footprinting of P vpsL complexes on ( A ) nontemplate DNA and ( B ) template DNA. GA corresponds to G+A ladder. VpsR, c-di-GMP and/or RNAP are present as indicated. To the right of each gel image, a schematic indicates the −10 and −35 regions and the +1. The VpsR binding site is indicated as a dashed black line. DNase I protection regions and hypersensitive sites seen with the activated complex of RNAP, VpsR, c-di-GMP and DNA are depicted as black rectangles and horizontal arrows, respectively. The dashed red boxes indicate the regions of DNA where the protection/enhancement within and immediately adjacent to the VpsR binding site changes when comparing complexes containing RNAP with or without VpsR or c-di-GMP to the activated complex. (See text for details.)

    Article Snippet: Cells were harvested by centrifugation and RNA was extracted using the RNeasy kit (Qiagen), and an on-column DNase I digestion (Qiagen) was performed according to manufacturer's instructions.

    Techniques: Footprinting, Binding Assay

    DNase I footprinting analysis of N. gonorrhoeae Fur protein with gonococcal fur and tonB promoter/operator regions. (A) DNase I footprint analysis of the fur promoter operator region. Radiolabeled DNA was incubated with increasing concentrations of Fur

    Journal: Journal of Bacteriology

    Article Title: Expression of the Gonococcal Global Regulatory Protein Fur and Genes Encompassing the Fur and Iron Regulon during In Vitro and In Vivo Infection in Women

    doi: 10.1128/JB.01830-07

    Figure Lengend Snippet: DNase I footprinting analysis of N. gonorrhoeae Fur protein with gonococcal fur and tonB promoter/operator regions. (A) DNase I footprint analysis of the fur promoter operator region. Radiolabeled DNA was incubated with increasing concentrations of Fur

    Article Snippet: On-column DNase I digestion was also performed for each sample according to the manufacturer's instructions (Qiagen).

    Techniques: Footprinting, Incubation

    DNase I footprinting assay of EsaR binding sites in the noncoding strand of P dkgA , coding strand of P glpF , and noncoding strand of P lrhA . (A to C) Capillary electrophoresis of FAM-labeled DNA fragments P dkgA (A), P glpF (B), and P lrhA (C) from DNase I

    Journal: Applied and Environmental Microbiology

    Article Title: Proteomic Analysis of the Quorum-Sensing Regulon in Pantoea stewartii and Identification of Direct Targets of EsaR

    doi: 10.1128/AEM.01744-13

    Figure Lengend Snippet: DNase I footprinting assay of EsaR binding sites in the noncoding strand of P dkgA , coding strand of P glpF , and noncoding strand of P lrhA . (A to C) Capillary electrophoresis of FAM-labeled DNA fragments P dkgA (A), P glpF (B), and P lrhA (C) from DNase I

    Article Snippet: The cell pellets were stored at −80°C prior to total RNA extraction using the RNeasy minikit (Qiagen) with an additional on-column DNase I digestion step using the RNase-free DNase set (Qiagen).

    Techniques: Footprinting, Binding Assay, Electrophoresis, Labeling

    Nested deletion EMSA analysis of EsaR direct targets. (A to C) The region protected by DNase I digestion in P dkgA (A), P glpF (B), and P lrhA (C) is the gray-shaded sequence (5′ to 3′), and the underlined bases are the 20-bp EsaR binding

    Journal: Applied and Environmental Microbiology

    Article Title: Proteomic Analysis of the Quorum-Sensing Regulon in Pantoea stewartii and Identification of Direct Targets of EsaR

    doi: 10.1128/AEM.01744-13

    Figure Lengend Snippet: Nested deletion EMSA analysis of EsaR direct targets. (A to C) The region protected by DNase I digestion in P dkgA (A), P glpF (B), and P lrhA (C) is the gray-shaded sequence (5′ to 3′), and the underlined bases are the 20-bp EsaR binding

    Article Snippet: The cell pellets were stored at −80°C prior to total RNA extraction using the RNeasy minikit (Qiagen) with an additional on-column DNase I digestion step using the RNase-free DNase set (Qiagen).

    Techniques: Sequencing, Binding Assay