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collagenase type ii  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical collagenase type ii
    Collagenase Type Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 14302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagenase/Collagenase%2C+Type+2/pmc12811436-116-41-44
    Average 99 stars, based on 14302 article reviews
    collagenase type ii - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Sensory-motor integration in a nonspiking interneuron contributes to active sensor control in Drosophila
    Article Snippet: .. We broke through the sheath of the brain and cleaned the area around the cell bodies of interest using a small amount of collagenase (5% in extracellular saline; Worthington Biochemical Corporation Collagenase Type 4), which we applied with a fine-tip electrode (approximately 5-10mm in diameter; item #TW150F-3, World Precision Instruments) at room temperature. ..

    Incubation:

    Article Title: Distinct tissue niches contribute to prostate tissue-resident memory CD8 + T cell differentiation and heterogeneity.
    Article Snippet: Then the entire tissue section was cut longitudinally, then cut laterally into 1 cm pieces, which were then incubated at 37◦C for 30 minutes in Hanks’ balanced salt solution with 2.4 mg ml− 1 of Hepes, 2.1 mg ml− 1 of sodium bicarbonate, 8% bovine growth serum and 0.154 mg ml− 1 of DTE (EMD Millipore). .. The SG, kidney, lung and liver were cut into small pieces then incubated with shaking at 37◦C for 30 minutes (SG, WAT, kidney liver) or 1 hour (lung) in RPMI with 1.2 mg ml− 1 of Hepes, 292 μg ml− 1 of L-glutamine, 1 mM MgCl2, 1 mM CaCl2, 5% FBS and 100 U ml− 1 of collagenase (Worthington). ..

    Article Title: Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1
    Article Snippet: .. For DRG explant cultures, DRGs were isolated from adult mice, placed in Dulbecco’s modified Eagle’s medium (DMEM)/F12 on ice, washed twice with ice-cold Hanks’ balanced salt solution (HBSS)–Hepes, and incubated in 0.6% collagenase (Worthington, # LS004196 ) for 45 min at 37°C. ..

    Article Title: Methods and compositions for treatment of pancreatic cancer
    Article Snippet: .. Cell Isolation Mouse and human PDAC tumors and adjacent pancreata were mechanically dissociated and incubated in collagenase (collagenase II for murine tumors, collagenase IV for human tumors, both 5 mg/ml; Worthington Biochemical Corp., Fisher Scientific), DNAse I (0.5 mg/ml; Roche Diagnostics), and Hank's balanced salt solution (Gibco, Fisher Scientific) for 30 minutes at 37° C. Digestion was then quenched with fetal bovine serum (FBS, Life Technologies), and cells were filtered sequentially through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific). .. Tumors, adjacent pancreata, and lymph nodes were then mechanically disassociated and filtered through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific) using PBS with 1% FBS (Life Technologies).

    Isolation:

    Article Title: Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1
    Article Snippet: .. For DRG explant cultures, DRGs were isolated from adult mice, placed in Dulbecco’s modified Eagle’s medium (DMEM)/F12 on ice, washed twice with ice-cold Hanks’ balanced salt solution (HBSS)–Hepes, and incubated in 0.6% collagenase (Worthington, # LS004196 ) for 45 min at 37°C. ..

    Modification:

    Article Title: Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1
    Article Snippet: .. For DRG explant cultures, DRGs were isolated from adult mice, placed in Dulbecco’s modified Eagle’s medium (DMEM)/F12 on ice, washed twice with ice-cold Hanks’ balanced salt solution (HBSS)–Hepes, and incubated in 0.6% collagenase (Worthington, # LS004196 ) for 45 min at 37°C. ..

    Purification:

    Article Title: Layered Single-Cell Heterogeneity in Hormone Receptor Signaling Across Mouse Organoids and Human ERα+ Cancer Cells
    Article Snippet: Dissociation mixture is prepared using the complete media composed of DMEM/F12 media (Gibco 11039-021), 5% Fetal Bovine Serum (CAT#), and 0.1% Gentamicin (Sigma G1397). .. 50 mg Collagenase (Type 4 filtered, Worthington LS004212) and 3 kunitz units Hyaluronidase (Purified, Worthington LS005475) are used to prepare 30 ml of dissociation mix. ..

    Cell Isolation:

    Article Title: Methods and compositions for treatment of pancreatic cancer
    Article Snippet: .. Cell Isolation Mouse and human PDAC tumors and adjacent pancreata were mechanically dissociated and incubated in collagenase (collagenase II for murine tumors, collagenase IV for human tumors, both 5 mg/ml; Worthington Biochemical Corp., Fisher Scientific), DNAse I (0.5 mg/ml; Roche Diagnostics), and Hank's balanced salt solution (Gibco, Fisher Scientific) for 30 minutes at 37° C. Digestion was then quenched with fetal bovine serum (FBS, Life Technologies), and cells were filtered sequentially through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific). .. Tumors, adjacent pancreata, and lymph nodes were then mechanically disassociated and filtered through 100- and 40-mm nylon cell strainers (Falcon, Fisher Scientific) using PBS with 1% FBS (Life Technologies).



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