collagenase  (Worthington Biochemical)


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    Name:
    Collagenase Type 4
    Description:
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    Catalog Number:
    ls004186
    Price:
    35
    Size:
    100 mg
    Source:
    Clostridium histolyticum
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase
    Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity Suggested for pancreatic islet primary isolation A dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase/product/Worthington Biochemical
    Average 99 stars, based on 232 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Myocardial infarction stabilization by cell‐based expression of controlled Vascular Endothelial Growth Factor levels
    Article Snippet: .. Tissue was minced and digested with 0.15% collagenase (Worthington Biochemical Corporation, Lakewood, NJ) in phosphate‐buffered saline (PBS) at 37°C under continuous shaking for 60 min. After centrifugation at 1500 rpm for 10 min, the lipid‐rich layer was discarded and the cellular pellet was washed once with PBS. .. Released cells were strained through a 100‐μm nylon‐mesh followed by a 70‐μm nylon‐mesh to remove fibrous debris, plated at a density of 105 cells/cm2 and cultured in high‐glucose Dulbecco's modified Eagle's medium with 10% foetal bovine serum, as described .

    Homogenization:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Isolation:

    Article Title: Altered mitochondrial quality control in Atg7-deficient VSMCs promotes enhanced apoptosis and is linked to unstable atherosclerotic plaque phenotype
    Article Snippet: .. Briefly, isolated aortas were incubated with Hank's Balanced Salt Solution (HBSS) containing 1 mg/ml collagenase (Worthington, type II CLS, 4176), 1 mg/mL trypsin inhibitor aprotinin, 0.74 units/mL elastase (Worthington, 2279) and 1% penicillin/streptomycin for 15 min at 37 °C in 5% CO2 . ..

    Purification:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Concentration Assay:

    Article Title: Electrical Homogenization of Ventricular Scar by Application of Collagenase: A Novel Strategy for Arrhythmia Therapy
    Article Snippet: .. Three types of clostridial CLGs [Type 2 collagenase (CLG-2); Type 4 collagenase (CLG-4); and purified collagenase (CLSPA); Worthington Biochemical Corporation, NJ] were evaluated to identify the optimal CLG subtype, and concentration for scar homogenization. ..

    Incubation:

    Article Title: Altered mitochondrial quality control in Atg7-deficient VSMCs promotes enhanced apoptosis and is linked to unstable atherosclerotic plaque phenotype
    Article Snippet: .. Briefly, isolated aortas were incubated with Hank's Balanced Salt Solution (HBSS) containing 1 mg/ml collagenase (Worthington, type II CLS, 4176), 1 mg/mL trypsin inhibitor aprotinin, 0.74 units/mL elastase (Worthington, 2279) and 1% penicillin/streptomycin for 15 min at 37 °C in 5% CO2 . ..

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  • 99
    Worthington Biochemical collagenase type 2
    The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and <t>type</t> 2 diabetic ( db/db ) under basal conditions and in response
    Collagenase Type 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type 2/product/Worthington Biochemical
    Average 99 stars, based on 445 article reviews
    Price from $9.99 to $1999.99
    collagenase type 2 - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Worthington Biochemical collagenase type i
    SMC migration was reduced in the absence of DDR1. SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to migrate for 4 hours in chemotaxis chambers with 200 nM <t>type</t> I collagen (left) or type VIII collagen (right) added to <t>DMEM</t> in the bottom of the chamber. Migration was quantified by staining and counting the number of cells that migrated to the bottom of the filter. Experiments were done in triplicate and repeated three times. A Values from DDR1–/– SMCs were significantly less than values from wild-type SMCs.
    Collagenase Type I, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 487 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type i/product/Worthington Biochemical
    Average 99 stars, based on 487 article reviews
    Price from $9.99 to $1999.99
    collagenase type i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and type 2 diabetic ( db/db ) under basal conditions and in response

    Journal: Life sciences

    Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart

    doi: 10.1016/j.lfs.2012.06.011

    Figure Lengend Snippet: The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and type 2 diabetic ( db/db ) under basal conditions and in response

    Article Snippet: Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington).

    Techniques: Western Blot

    The temporal progression of body weight (A) and survival (B) following either sham or trans-aortic constriction surgery to induce a pressure overload (PO) in non-diabetic (control), high fat-fed, or high fat-fed and STZ treated type 2 diabetic rats. †

    Journal: Life sciences

    Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart

    doi: 10.1016/j.lfs.2012.06.011

    Figure Lengend Snippet: The temporal progression of body weight (A) and survival (B) following either sham or trans-aortic constriction surgery to induce a pressure overload (PO) in non-diabetic (control), high fat-fed, or high fat-fed and STZ treated type 2 diabetic rats. †

    Article Snippet: Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington).

    Techniques:

    Proposed model of endocrine therapy effects on adipose tissue. ERα signaling maintains adipocyte progenitor pools and inhibits preadipocyte expansion, adipocyte differentiation, and hypertrophy. Disruption of E 2 signaling through either tamoxifen treatment or withdrawal of E 2 depletes the adipocyte progenitor pool causing adipocyte hypertrophy consistent with a phenotype that precedes insulin resistance and the development of type 2 diabetes.

    Journal: bioRxiv

    Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance

    doi: 10.1101/2020.08.21.259440

    Figure Lengend Snippet: Proposed model of endocrine therapy effects on adipose tissue. ERα signaling maintains adipocyte progenitor pools and inhibits preadipocyte expansion, adipocyte differentiation, and hypertrophy. Disruption of E 2 signaling through either tamoxifen treatment or withdrawal of E 2 depletes the adipocyte progenitor pool causing adipocyte hypertrophy consistent with a phenotype that precedes insulin resistance and the development of type 2 diabetes.

    Article Snippet: Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase).

    Techniques:

    SMC migration was reduced in the absence of DDR1. SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to migrate for 4 hours in chemotaxis chambers with 200 nM type I collagen (left) or type VIII collagen (right) added to DMEM in the bottom of the chamber. Migration was quantified by staining and counting the number of cells that migrated to the bottom of the filter. Experiments were done in triplicate and repeated three times. A Values from DDR1–/– SMCs were significantly less than values from wild-type SMCs.

    Journal: Journal of Clinical Investigation

    Article Title: The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair

    doi:

    Figure Lengend Snippet: SMC migration was reduced in the absence of DDR1. SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to migrate for 4 hours in chemotaxis chambers with 200 nM type I collagen (left) or type VIII collagen (right) added to DMEM in the bottom of the chamber. Migration was quantified by staining and counting the number of cells that migrated to the bottom of the filter. Experiments were done in triplicate and repeated three times. A Values from DDR1–/– SMCs were significantly less than values from wild-type SMCs.

    Article Snippet: The vessels (12 vessels were pooled per dish) were then transferred to fresh dispersion media: DMEM with 1% HEPES, 1% penicillin-streptomycin, 1.8 mg/ml collagenase type I (Worthington Biochemical Co., Freehold, New Jersey, USA), 0.3 mg/ml elastase type III, 0.44 mg/ml soybean trypsin-inhibitor type I, and 2 mg/ml BSA.

    Techniques: Migration, Mouse Assay, Chemotaxis Assay, Staining

    Cell proliferation on collagen was less in DDR1–/– SMCs compared with DDR1+/+ SMCs. ( a ) SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to grow with 10% FCS and incubated with [ 3 H]thymidine on wells coated with 100 nM type I collagen (left) or type VIII collagen (right). A Value from DDR1–/– SMCs was significantly less than value from wild-type SMCs. ( b ) Number of SMCs per well after plating on 100 nM type I collagen and growing in DMEM containing 10% FCS for 1–4 days. Squares, DDR1+/+ SMCs; circles, DDR1–/– SMCs. Experiments were done in triplicate and repeated three times. A Value from DDR1–/– SMCs was significantly less than value from wild-type SMCs.

    Journal: Journal of Clinical Investigation

    Article Title: The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair

    doi:

    Figure Lengend Snippet: Cell proliferation on collagen was less in DDR1–/– SMCs compared with DDR1+/+ SMCs. ( a ) SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to grow with 10% FCS and incubated with [ 3 H]thymidine on wells coated with 100 nM type I collagen (left) or type VIII collagen (right). A Value from DDR1–/– SMCs was significantly less than value from wild-type SMCs. ( b ) Number of SMCs per well after plating on 100 nM type I collagen and growing in DMEM containing 10% FCS for 1–4 days. Squares, DDR1+/+ SMCs; circles, DDR1–/– SMCs. Experiments were done in triplicate and repeated three times. A Value from DDR1–/– SMCs was significantly less than value from wild-type SMCs.

    Article Snippet: The vessels (12 vessels were pooled per dish) were then transferred to fresh dispersion media: DMEM with 1% HEPES, 1% penicillin-streptomycin, 1.8 mg/ml collagenase type I (Worthington Biochemical Co., Freehold, New Jersey, USA), 0.3 mg/ml elastase type III, 0.44 mg/ml soybean trypsin-inhibitor type I, and 2 mg/ml BSA.

    Techniques: Mouse Assay, Incubation

    Product of DSP protein coated on glass slides. A . Coomassie-stained SDS-PAGE gel for analysis of expression of the recombinant COOH-terminal dentin sialoprotein (rC-DSP) protein. The sample in DL21 cells was grown 3 h with 1 mM IPTG induction after reaching O.D.600 of 0.6. The rC-DSP was purified by the column described in the materials and methods. B . Expression of purified rC-DSP was confirmed by Western blotting analysis using goat polyclonal anti-DSP antibody (Santa Cruz Biotechnology Inc.). D - E . Collagen type I and rC-DSP proteins were coated on glass slides for overnight at 37°C. For scanning electron microscopy, the samples were washed and dried as well as treated with sodium deoxycholate. Then the samples were observed under scanning electron microscope (SEM). Fibril matrix was visible on the collagen type I coated slide ( D ) and rC-DSP coated slide ( E ), but no control slide ( C ). Also, there were different morphologies between rC-DSP and collagen type I.

    Journal: PLoS ONE

    Article Title: Domain of Dentine Sialoprotein Mediates Proliferation and Differentiation of Human Periodontal Ligament Stem Cells

    doi: 10.1371/journal.pone.0081655

    Figure Lengend Snippet: Product of DSP protein coated on glass slides. A . Coomassie-stained SDS-PAGE gel for analysis of expression of the recombinant COOH-terminal dentin sialoprotein (rC-DSP) protein. The sample in DL21 cells was grown 3 h with 1 mM IPTG induction after reaching O.D.600 of 0.6. The rC-DSP was purified by the column described in the materials and methods. B . Expression of purified rC-DSP was confirmed by Western blotting analysis using goat polyclonal anti-DSP antibody (Santa Cruz Biotechnology Inc.). D - E . Collagen type I and rC-DSP proteins were coated on glass slides for overnight at 37°C. For scanning electron microscopy, the samples were washed and dried as well as treated with sodium deoxycholate. Then the samples were observed under scanning electron microscope (SEM). Fibril matrix was visible on the collagen type I coated slide ( D ) and rC-DSP coated slide ( E ), but no control slide ( C ). Also, there were different morphologies between rC-DSP and collagen type I.

    Article Snippet: These cells were digested for 1 h at 37°C in a solution of 3 mg⁄ml collagenase type I and 4 mg⁄ml of dispase (Worthington Biochem, Freehold, NJ).

    Techniques: Staining, SDS Page, Expressing, Recombinant, Purification, Western Blot, Electron Microscopy, Microscopy