Structured Review

Millipore collagenase
Collagenase, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
collagenase - by Bioz Stars, 2021-05
86/100 stars

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Related Articles

Cell Culture:

Article Title: eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet
Article Snippet: Cell culture and treatments Immortalized wild-type (HEP S/S ) and homozygous mutant (HEP A/A ) hepatocytes were cultured as described previously [ ]. .. Primary mouse hepatocytes were obtained by liver perfusion with collagenase IV (Sigma Aldrich, C5138), inoculated on collagen-coated plates with/without round coverslips (5 × 105 cells/well in 6-well plates), and cultured in Dulbecco’s modified Eagle’s medium (DMEM, high glucose) medium containing 10% FBS, and penicillin/streptomycin. .. The medium was replaced with FBS-free DMEM media 2 h after plating and the hepatocytes were incubated for a further 12 h before experimental treatment.

Modification:

Article Title: eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet
Article Snippet: Cell culture and treatments Immortalized wild-type (HEP S/S ) and homozygous mutant (HEP A/A ) hepatocytes were cultured as described previously [ ]. .. Primary mouse hepatocytes were obtained by liver perfusion with collagenase IV (Sigma Aldrich, C5138), inoculated on collagen-coated plates with/without round coverslips (5 × 105 cells/well in 6-well plates), and cultured in Dulbecco’s modified Eagle’s medium (DMEM, high glucose) medium containing 10% FBS, and penicillin/streptomycin. .. The medium was replaced with FBS-free DMEM media 2 h after plating and the hepatocytes were incubated for a further 12 h before experimental treatment.

Mouse Assay:

Article Title: Mutation of the palmitoylation site of estrogen receptor ? in vivo reveals tissue-specific roles for membrane versus nuclear actions
Article Snippet: Primary hepatocyte cultures were isolated from the livers of 8- to10-wk-old mice by a modification of the collagenase method ( ). .. Briefly, livers from WT-C451 and C451A-ERα mice were perfused using the portal vein with HBSS before collagenase perfusion (C5138; Sigma). ..

Incubation:

Article Title: Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis
Article Snippet: Blood was treated with 1× RBC solution (00-4333-57, eBioscience) to lyse red blood cells. .. Spleen and DLN were dissociated in RPMI 1640 medium containing 10% FBS, 200 μg/ml Collagenase IV (DN25, Sigma), and 100 μg/ml DNase I (C5138, Sigma) with the gentleMACS™ Octo Dissociator, and then incubated at 37°C, 200 rpm for 15 and 30 min, respectively. .. Mouse psoriatic skin was cut into small pieces of 2~4 mm with scissors and incubated for 100 min at 37°C, 180 rpm with basic Hank’s Balanced Salt Solution (HBSS, 14065-056, Gibco) containing 250 μg/ml Liberase TL (5401020001, Roche) and 500 μg/ml DNase I, then transferred into the gentleMACS C tube to dissociation with the gentleMACS™ Octo Dissociator at 37°C for 5 min. All samples were respectively filtered into 15 ml tubes with MACS SmartStrainer (30 μm) to obtain single cell suspension.

Article Title: Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function
Article Snippet: Epithelial cells and mucus were removed by 40 min incubation with 5 ml HBSS−/− (without Ca2+ and Mg2+ ) containing 5% FBS, 2 mM EDTA, and 1 mM DTT (Sigma) at 37°C shaking at 250 rpm. .. Colon pieces were then incubated with shaking (37°C, 250 rpm for 45 min) with 5 ml digestion buffer [5% FCS, 1 mg/ml collagenase VIII (Sigma-Aldrich, Rehovot, Israel, C5138-500MG) in PBS+/+ ]. ..

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  • 97
    Millipore clostridium histolyticum
    Degradation of human type I collagen (A) and elastin (B) by the cell-associated complex and the monomeric forms of Rgp and Kgp. FITC-labeled collagen and elastin-fluorescein were incubated with the complex or the monomeric Rgp and/or Kgp in the presence or absence of Rgp and/or Kgp inhibitors as described in Materials and Methods. The collagenase from C. <t>histolyticum</t> was used as the positive control with the same protein amount as the complex. The values are expressed as a ratio to the activity obtained by treatment with the combination of monomeric Rgp and Kgp. RK, a combination of the monomeric forms of Rgp and Kgp; C, the complex; CL, the complex in the presence of leupeptin; CK1, the complex in the presence of KYT-1 (Rgp inhibitor); CK36, the complex in the presence of KYT-36 (Kgp inhibitor); CK1/36, the complex in the presence of KYT-1 and KYT-36; H, collagenase from C. histolyticum . * P
    Clostridium Histolyticum, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clostridium histolyticum/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clostridium histolyticum - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    97
    Millipore collagenase 1a
    Quantification of hydrogel degradation kinetics. Degradation kinetics of hydrogel sections taken from different regions of (A) bulk polymerized control gels and (B) PBFP gradient hydrogels when exposed to 1 µg/mL <t>collagenase-1A</t> (MMP-1) (n = 3). Error bars represent ± standard deviation.
    Collagenase 1a, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase 1a/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase 1a - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    97
    Millipore collagenase type i
    Cleavage of FLNa regulates FA turnover and cell invasion. (A) BT-20 siFLNa cells were transfected with construct either expressing GFP-tagged FLNa WT (GFP–FLNa-WT) or GFP-tagged FLNa mutant resistant to calpain cleavage (GFP–FLNa-Δ) and assayed for <t>nocodazole</t> (NZ)-based FA disassembly. Cells were untreated (control) or incubated with 10 µM nocodazole for 4 h. Cell lysates were collected at the indicated times and immunoblotted with anti–FAK-pY397 or anti–paxillin-pY118. (B) BT-20 siFLNa cells transfected with either GFP–FLNa-WT or GFP–FLNa-Δ were cultured on 10-cm dishes and stimulated or not with degraded <t>type</t> I collagen (De.Collagen) for the indicated times. Where indicated, cells were treated with 50 µM of calpain inhibitor ALLN (22 h). Lysates were prepared at the indicated time points and analyzed by Western blotting using the corresponding antibodies. Control indicates unstimulated. NT, N terminus. (C) Control BT-20 cells, BT-20 siFLNa cells, and BT-20 siFLNa cells expressing GFP–FLNa-WT or GFP–FLNa-Δ were placed in the top layer of Boyden migration chamber, and after 48 h, cells migrated through the chamber to the bottom membrane were measured. The graph represents the mean ± SD from four independent experiments (*, P
    Collagenase Type I, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type i/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type i - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier


    Image Search Results


    Degradation of human type I collagen (A) and elastin (B) by the cell-associated complex and the monomeric forms of Rgp and Kgp. FITC-labeled collagen and elastin-fluorescein were incubated with the complex or the monomeric Rgp and/or Kgp in the presence or absence of Rgp and/or Kgp inhibitors as described in Materials and Methods. The collagenase from C. histolyticum was used as the positive control with the same protein amount as the complex. The values are expressed as a ratio to the activity obtained by treatment with the combination of monomeric Rgp and Kgp. RK, a combination of the monomeric forms of Rgp and Kgp; C, the complex; CL, the complex in the presence of leupeptin; CK1, the complex in the presence of KYT-1 (Rgp inhibitor); CK36, the complex in the presence of KYT-36 (Kgp inhibitor); CK1/36, the complex in the presence of KYT-1 and KYT-36; H, collagenase from C. histolyticum . * P

    Journal: Infection and Immunity

    Article Title: A Functional Virulence Complex Composed of Gingipains, Adhesins, and Lipopolysaccharide Shows High Affinity to Host Cells and Matrix Proteins and Escapes Recognition by Host Immune Systems

    doi: 10.1128/IAI.73.2.883-893.2005

    Figure Lengend Snippet: Degradation of human type I collagen (A) and elastin (B) by the cell-associated complex and the monomeric forms of Rgp and Kgp. FITC-labeled collagen and elastin-fluorescein were incubated with the complex or the monomeric Rgp and/or Kgp in the presence or absence of Rgp and/or Kgp inhibitors as described in Materials and Methods. The collagenase from C. histolyticum was used as the positive control with the same protein amount as the complex. The values are expressed as a ratio to the activity obtained by treatment with the combination of monomeric Rgp and Kgp. RK, a combination of the monomeric forms of Rgp and Kgp; C, the complex; CL, the complex in the presence of leupeptin; CK1, the complex in the presence of KYT-1 (Rgp inhibitor); CK36, the complex in the presence of KYT-36 (Kgp inhibitor); CK1/36, the complex in the presence of KYT-1 and KYT-36; H, collagenase from C. histolyticum . * P

    Article Snippet: Collagenase from Clostridium histolyticum (type I; Sigma) was used as a positive control.

    Techniques: Labeling, Incubation, Positive Control, Activity Assay

    Quantification of hydrogel degradation kinetics. Degradation kinetics of hydrogel sections taken from different regions of (A) bulk polymerized control gels and (B) PBFP gradient hydrogels when exposed to 1 µg/mL collagenase-1A (MMP-1) (n = 3). Error bars represent ± standard deviation.

    Journal: PLoS ONE

    Article Title: MMP-Sensitive PEG Diacrylate Hydrogels with Spatial Variations in Matrix Properties Stimulate Directional Vascular Sprout Formation

    doi: 10.1371/journal.pone.0058897

    Figure Lengend Snippet: Quantification of hydrogel degradation kinetics. Degradation kinetics of hydrogel sections taken from different regions of (A) bulk polymerized control gels and (B) PBFP gradient hydrogels when exposed to 1 µg/mL collagenase-1A (MMP-1) (n = 3). Error bars represent ± standard deviation.

    Article Snippet: Collagenase-1A (Sigma Aldrich) at a concentration of 1 µg/mL was added to the hydrogel sections and the swollen weight of each section was recorded every two hours until complete hydrogel degradation was achieved.

    Techniques: Standard Deviation

    Cleavage of FLNa regulates FA turnover and cell invasion. (A) BT-20 siFLNa cells were transfected with construct either expressing GFP-tagged FLNa WT (GFP–FLNa-WT) or GFP-tagged FLNa mutant resistant to calpain cleavage (GFP–FLNa-Δ) and assayed for nocodazole (NZ)-based FA disassembly. Cells were untreated (control) or incubated with 10 µM nocodazole for 4 h. Cell lysates were collected at the indicated times and immunoblotted with anti–FAK-pY397 or anti–paxillin-pY118. (B) BT-20 siFLNa cells transfected with either GFP–FLNa-WT or GFP–FLNa-Δ were cultured on 10-cm dishes and stimulated or not with degraded type I collagen (De.Collagen) for the indicated times. Where indicated, cells were treated with 50 µM of calpain inhibitor ALLN (22 h). Lysates were prepared at the indicated time points and analyzed by Western blotting using the corresponding antibodies. Control indicates unstimulated. NT, N terminus. (C) Control BT-20 cells, BT-20 siFLNa cells, and BT-20 siFLNa cells expressing GFP–FLNa-WT or GFP–FLNa-Δ were placed in the top layer of Boyden migration chamber, and after 48 h, cells migrated through the chamber to the bottom membrane were measured. The graph represents the mean ± SD from four independent experiments (*, P

    Journal: The Journal of Experimental Medicine

    Article Title: Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion

    doi: 10.1084/jem.20100433

    Figure Lengend Snippet: Cleavage of FLNa regulates FA turnover and cell invasion. (A) BT-20 siFLNa cells were transfected with construct either expressing GFP-tagged FLNa WT (GFP–FLNa-WT) or GFP-tagged FLNa mutant resistant to calpain cleavage (GFP–FLNa-Δ) and assayed for nocodazole (NZ)-based FA disassembly. Cells were untreated (control) or incubated with 10 µM nocodazole for 4 h. Cell lysates were collected at the indicated times and immunoblotted with anti–FAK-pY397 or anti–paxillin-pY118. (B) BT-20 siFLNa cells transfected with either GFP–FLNa-WT or GFP–FLNa-Δ were cultured on 10-cm dishes and stimulated or not with degraded type I collagen (De.Collagen) for the indicated times. Where indicated, cells were treated with 50 µM of calpain inhibitor ALLN (22 h). Lysates were prepared at the indicated time points and analyzed by Western blotting using the corresponding antibodies. Control indicates unstimulated. NT, N terminus. (C) Control BT-20 cells, BT-20 siFLNa cells, and BT-20 siFLNa cells expressing GFP–FLNa-WT or GFP–FLNa-Δ were placed in the top layer of Boyden migration chamber, and after 48 h, cells migrated through the chamber to the bottom membrane were measured. The graph represents the mean ± SD from four independent experiments (*, P

    Article Snippet: Fibronectin, collagenase type I, 10× RPMI 1640, and nocodazole were purchased from Sigma-Aldrich.

    Techniques: Transfection, Construct, Expressing, Mutagenesis, Incubation, Cell Culture, Western Blot, Migration

    Therapeutic modulation of miR-146b-5p relieves cardiac fibrosis and remodeling in MI pig. (A) The LVEF of the experimental pigs was analyzed using echocardiography at different time points (sham group: n = 4, other groups: n = 8 each). (B) The survival analysis of the experimental groups. The survival curve was plotted using the Kaplan-Meier method. Pigs were sacrificed 4 weeks after MI. (C) Representative slices of the porcine myocardium. Infarction areas are indicated by the blue dashed line. Bar = 1 cm. (D) Infarction area analysis. Five whole heart sections per group were randomly selected, and the percentage of infarction area to total area in cross section was calculated. (E–G) The representative images for histological and immunohistochemical staining are shown (10 random fields per group, bar = 100 μm), indicating fibrosis, as per Masson’s staining (E); expression of collagen type 1 (Col1, F); and CD31 + capillaries (G). Cell apoptosis was analyzed by TUNEL staining (Fig. S5). Data are expressed as the mean ± SD. * P

    Journal: Protein & Cell

    Article Title: Therapeutic silencing miR-146b-5p improves cardiac remodeling in a porcine model of myocardial infarction by modulating the wound reparative phenotype

    doi: 10.1007/s13238-020-00750-6

    Figure Lengend Snippet: Therapeutic modulation of miR-146b-5p relieves cardiac fibrosis and remodeling in MI pig. (A) The LVEF of the experimental pigs was analyzed using echocardiography at different time points (sham group: n = 4, other groups: n = 8 each). (B) The survival analysis of the experimental groups. The survival curve was plotted using the Kaplan-Meier method. Pigs were sacrificed 4 weeks after MI. (C) Representative slices of the porcine myocardium. Infarction areas are indicated by the blue dashed line. Bar = 1 cm. (D) Infarction area analysis. Five whole heart sections per group were randomly selected, and the percentage of infarction area to total area in cross section was calculated. (E–G) The representative images for histological and immunohistochemical staining are shown (10 random fields per group, bar = 100 μm), indicating fibrosis, as per Masson’s staining (E); expression of collagen type 1 (Col1, F); and CD31 + capillaries (G). Cell apoptosis was analyzed by TUNEL staining (Fig. S5). Data are expressed as the mean ± SD. * P

    Article Snippet: Briefly, minced mouse myocardium tissue was digested using 1 mg/mL warm collagenase type 1 (C5849, Sigma), 5 units/mL dispase (345235, Collaborative), and 60 units/mL DNase 1 (10104159001, Roche) for 45 min. After filtration through a 70-μm cell strainer, single cell pellets were incubated with anti-CD31 magnetic microbeads (antibody: 553370, BD; microbeads: 11035, Invitrogen) for 20 min following the manufacturer’s instructions.

    Techniques: Immunohistochemistry, Staining, Expressing, TUNEL Assay