collagenase  (Millipore)


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    Structured Review

    Millipore collagenase
    Collagenase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase/product/Millipore
    Average 99 stars, based on 2385 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Inhibition of cholinergic potentiation of insulin secretion from pancreatic islets by chronic elevation of glucose and fatty acids: Protection by casein kinase 2 inhibitor
    Article Snippet: .. 2.2 Mouse islet: isolation and culture Mouse islets from male mice were isolated from fed animals using collagenase (EC 3.4.24.3 Serva, 17449) digestion in Hanks buffer followed by separation of islets from exocrine tissue in a Ficoll (Sigma, F-9378) gradient. .. Isolated islets were cultured for 3–5 days in RPMI 1640 medium (Sigma) containing 10% undialized fetal bovine serum, 10 ml/l penicillin-streptomycin-amphotericin B solution (GIBCO BRL) and 10, 16, or 25 mM glucose with or without free fatty acids noting that undialized bovine albumin contributes variable amounts, but at most 1 mM glucose to the culture medium.

    Incubation:

    Article Title: Multi-walled carbon nanotube oxidation dependent keratinocyte cytotoxicity and skin inflammation
    Article Snippet: .. An additional 1 mL of 0.1 U Collagenase/ 0.8 U Dispase (Sigma Aldrich Cat# 11097113001) was added to the skin samples along with 0.3 M calcium chloride, and the samples were incubated at 37o C for 30 min. After 30 min, the 0.5 M EDTA was added to the reaction to halt enzymatic activity. ..

    other:

    Article Title: Ca2+ and K+ channels of normal human adrenal zona fasciculata cells: Properties and modulation by ACTH and AngII
    Article Snippet: PBS, 1,2 bis -(2-aminophenoxy)ethane-N ,N ,N ′,N ″-tetraacetic acid (BAPTA), MgATP, collagenase (C0130), DNase, forskolin, ACTH (1–24), AngII, GdCl3 , NiCl2 , mibefradil, and arachidonic acid (AA) were obtained from Sigma-Aldrich.

    Article Title: Biocompatibility and degradation of tendon-derived scaffolds
    Article Snippet: Each sample was placed into a 24 well plate and 0.5 ml 0.1 M Tris–HCl with 0.005M CaCl2 (pH 7.4) added to hydrate the samples for 20 min. To this 0.5 ml of a collagenase solutions containing 2 mg/ml collagenase (Clostridium histolyticum (125CDU/mg), Sigma) in the same Tris–HCl buffer.

    Activity Assay:

    Article Title: Multi-walled carbon nanotube oxidation dependent keratinocyte cytotoxicity and skin inflammation
    Article Snippet: .. An additional 1 mL of 0.1 U Collagenase/ 0.8 U Dispase (Sigma Aldrich Cat# 11097113001) was added to the skin samples along with 0.3 M calcium chloride, and the samples were incubated at 37o C for 30 min. After 30 min, the 0.5 M EDTA was added to the reaction to halt enzymatic activity. ..

    Mouse Assay:

    Article Title: Inhibition of cholinergic potentiation of insulin secretion from pancreatic islets by chronic elevation of glucose and fatty acids: Protection by casein kinase 2 inhibitor
    Article Snippet: .. 2.2 Mouse islet: isolation and culture Mouse islets from male mice were isolated from fed animals using collagenase (EC 3.4.24.3 Serva, 17449) digestion in Hanks buffer followed by separation of islets from exocrine tissue in a Ficoll (Sigma, F-9378) gradient. .. Isolated islets were cultured for 3–5 days in RPMI 1640 medium (Sigma) containing 10% undialized fetal bovine serum, 10 ml/l penicillin-streptomycin-amphotericin B solution (GIBCO BRL) and 10, 16, or 25 mM glucose with or without free fatty acids noting that undialized bovine albumin contributes variable amounts, but at most 1 mM glucose to the culture medium.

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    Millipore human mmp 1
    <t>MMP-1</t> and TIMP-1 in human skin exposed to Omniscan. Biopsies were incubated for three days under control conditions or in the presence of Omniscan (250 μM). At the end of the incubation period, culture fluid was collected and assayed as follows:
    Human Mmp 1, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mmp 1/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    human mmp 1 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Millipore mmp 1 9 collagenase activity cells
    Downregulation of EMT markers after A , top panel ) Media from S2-013 cells, treated with indicated compounds was separated on a zymogram gel to analyze the proteolytic activity of <t>MMP-1/-9.</t> ( A , bottom graph ) densitometric analysis of zymogram gel in top panel , reveals a significant decrease in collagenase activity of MMP-1/-9 (**p
    Mmp 1 9 Collagenase Activity Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp 1 9 collagenase activity cells/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    MMP-1 and TIMP-1 in human skin exposed to Omniscan. Biopsies were incubated for three days under control conditions or in the presence of Omniscan (250 μM). At the end of the incubation period, culture fluid was collected and assayed as follows:

    Journal: Investigative radiology

    Article Title: Responses of Human Skin in Organ Culture and Human Skin Fibroblasts to a Gadolinium-Based MRI Contrast Agent: Comparison of Skin from Patients with End-Stage Renal Disease and Skin from Healthy Subjects

    doi: 10.1097/RLI.0b013e3181e9436b

    Figure Lengend Snippet: MMP-1 and TIMP-1 in human skin exposed to Omniscan. Biopsies were incubated for three days under control conditions or in the presence of Omniscan (250 μM). At the end of the incubation period, culture fluid was collected and assayed as follows:

    Article Snippet: Human MMP-1 (collagenase-1) was obtained from Calbiochem.

    Techniques: Incubation

    Collagen degradation upon exposure to skin organ culture fluid or fibroblast MMP-1. Upper panels : SDS-PAGE analysis of the collagen fragmentation pattern obtained following exposure of intact collagen lattices to control buffer alone, to two concentrations of skin organ culture fluid, or to activated MMP-1. The collagen lattices exposed to buffer alone ( control lanes ) show a faint doublet corresponding to the α 1 and α 2 chains of intact type I collagen. The bands are faint because most of the intact collagen remains polymerized in the lattice. The collagen lattices exposed to skin culture fluid show bands corresponding to the ¾- and ¼-sized fragments of type I collagen. Additional fragments are observed. The collagen lattice exposed to purified MMP-1 shows the ¾- and ¼-sized fragments. Middle panel : standard curve obtained by inducing complete hydrolysis of the collagen lattice with 75 ng of MMP-1 and resolving graded amounts of hydrolysate by SDS-PAGE. The ¾- and ¼-sized fragments were digitized and digital images quantified. Lower panels : scanning electron microscopic images of lattices exposed to buffer alone ( intact ) and to organ culture fluid ( fragmented ). Similar results were obtained with 10 different preparations of skin organ culture fluid

    Journal: Archives of dermatological research

    Article Title: Impaired keratinocyte function on matrix metalloproteinase-1 (MMP-1) damaged collagen

    doi: 10.1007/s00403-009-0948-4

    Figure Lengend Snippet: Collagen degradation upon exposure to skin organ culture fluid or fibroblast MMP-1. Upper panels : SDS-PAGE analysis of the collagen fragmentation pattern obtained following exposure of intact collagen lattices to control buffer alone, to two concentrations of skin organ culture fluid, or to activated MMP-1. The collagen lattices exposed to buffer alone ( control lanes ) show a faint doublet corresponding to the α 1 and α 2 chains of intact type I collagen. The bands are faint because most of the intact collagen remains polymerized in the lattice. The collagen lattices exposed to skin culture fluid show bands corresponding to the ¾- and ¼-sized fragments of type I collagen. Additional fragments are observed. The collagen lattice exposed to purified MMP-1 shows the ¾- and ¼-sized fragments. Middle panel : standard curve obtained by inducing complete hydrolysis of the collagen lattice with 75 ng of MMP-1 and resolving graded amounts of hydrolysate by SDS-PAGE. The ¾- and ¼-sized fragments were digitized and digital images quantified. Lower panels : scanning electron microscopic images of lattices exposed to buffer alone ( intact ) and to organ culture fluid ( fragmented ). Similar results were obtained with 10 different preparations of skin organ culture fluid

    Article Snippet: Human MMP-1 (collagenase-1) was obtained from Calbiochem (San Diego, CA).

    Techniques: Organ Culture, SDS Page, Purification

    A representative blot of the various forms of MMP13 following the treatment of estradiol plus and progesterone in the absence and presence of either proteasome inhibitor (MG-132, 5 μmol/L) or collagenase inhibitor I (CI, 5 μmol/L), demonstrating inhibition of fragmentation by MG-132 but not CI. E2P4, 17-β-estradiol (10 −8 mol/L) plus progesterone (10 −10 mol/L); kilodalton (kDa), labeled as Kd.

    Journal: Biology of Reproduction

    Article Title: The Amount and Activity of Active Matrix Metalloproteinase 13 Is Suppressed by Estradiol and Progesterone in Human Pelvic Floor Fibroblasts 1

    doi: 10.1095/biolreprod.108.072462

    Figure Lengend Snippet: A representative blot of the various forms of MMP13 following the treatment of estradiol plus and progesterone in the absence and presence of either proteasome inhibitor (MG-132, 5 μmol/L) or collagenase inhibitor I (CI, 5 μmol/L), demonstrating inhibition of fragmentation by MG-132 but not CI. E2P4, 17-β-estradiol (10 −8 mol/L) plus progesterone (10 −10 mol/L); kilodalton (kDa), labeled as Kd.

    Article Snippet: Fibroblasts at 95% confluence were incubated with hormone-free media containing 0.2% (w/v) LAH for 24 h and then pretreated with MG-132 (an inhibitor of ubiquitin-proteasomal proteolysis, 5 μmol/L, BostonBiochem, Cambridge, MA) or collagenase inhibitor I (CI, an inhibitor of MMP autocatalysis, 5 μmol/L, EMD Biosciences Inc.) for 1 h followed by the addition of E2 (10−8 mol/L) + P4 (10−10 mol/L).

    Techniques: Inhibition, Labeling

    A ) A representative blot of the expression of ubiquitinated proteins following a treatment with E2 + P4 in the presence of proteasome inhibitor (MG-132, 5 μmol/L) or collagenase inhibitor I (CI, 5 μmol/L). B ) Densitometric analysis demonstrated an accumulation of ubiquitinated proteins (higher density of the whole lane) in the presence of MG-132, but not CI, indicating a blockage of the ubiquitin-proteasome pathway. Error bars indicate ± SD. * P

    Journal: Biology of Reproduction

    Article Title: The Amount and Activity of Active Matrix Metalloproteinase 13 Is Suppressed by Estradiol and Progesterone in Human Pelvic Floor Fibroblasts 1

    doi: 10.1095/biolreprod.108.072462

    Figure Lengend Snippet: A ) A representative blot of the expression of ubiquitinated proteins following a treatment with E2 + P4 in the presence of proteasome inhibitor (MG-132, 5 μmol/L) or collagenase inhibitor I (CI, 5 μmol/L). B ) Densitometric analysis demonstrated an accumulation of ubiquitinated proteins (higher density of the whole lane) in the presence of MG-132, but not CI, indicating a blockage of the ubiquitin-proteasome pathway. Error bars indicate ± SD. * P

    Article Snippet: Fibroblasts at 95% confluence were incubated with hormone-free media containing 0.2% (w/v) LAH for 24 h and then pretreated with MG-132 (an inhibitor of ubiquitin-proteasomal proteolysis, 5 μmol/L, BostonBiochem, Cambridge, MA) or collagenase inhibitor I (CI, an inhibitor of MMP autocatalysis, 5 μmol/L, EMD Biosciences Inc.) for 1 h followed by the addition of E2 (10−8 mol/L) + P4 (10−10 mol/L).

    Techniques: Expressing

    Downregulation of EMT markers after A , top panel ) Media from S2-013 cells, treated with indicated compounds was separated on a zymogram gel to analyze the proteolytic activity of MMP-1/-9. ( A , bottom graph ) densitometric analysis of zymogram gel in top panel , reveals a significant decrease in collagenase activity of MMP-1/-9 (**p

    Journal: Oncotarget

    Article Title: Galeterone and its analogs inhibit Mnk-eIF4E axis, synergize with gemcitabine, impede pancreatic cancer cell migration, invasion and proliferation and inhibit tumor growth in mice

    doi: 10.18632/oncotarget.14154

    Figure Lengend Snippet: Downregulation of EMT markers after A , top panel ) Media from S2-013 cells, treated with indicated compounds was separated on a zymogram gel to analyze the proteolytic activity of MMP-1/-9. ( A , bottom graph ) densitometric analysis of zymogram gel in top panel , reveals a significant decrease in collagenase activity of MMP-1/-9 (**p

    Article Snippet: Analysis of secreted MMP-1/-9 collagenase activity Cells were treated with ARDAs or gemcitabine in phenol red free media, with no penicillin/streptomycin for 72 h. Cultured media from was collected and concentrated with 0.5 ml amicon ultra centrifugal columns (Millipore, Bedford, MA).

    Techniques: Activity Assay