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Boehringer Mannheim collagenase
Collagenase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
collagenase - by Bioz Stars, 2021-05
86/100 stars

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Related Articles

Centrifugation:

Article Title: Effects of calcium buffering on glucose-induced insulin release in mouse pancreatic islets: an approximation to the calcium sensor
Article Snippet: Islets from adult (8- to 10-week-old) Swiss albino male mice (OF1) killed by cervical dislocation in accordance with national guidelines were isolated as previously described ( ). .. Briefly, after pancreas digestion with collagenase (Collagenase A, Boehringer Mannheim) in a stationary bath at 37°C, islets were separated by centrifugation and hand picked under a stereomicroscope. .. Once isolated, islets were dissociated into single cells in a low-calcium medium as previously described ( ).

Article Title: Zn2+ chelation by serum albumin improves hexameric Zn2+-insulin dissociation into monomers after exocytosis
Article Snippet: Islet isolation Islets from adult (8–10 weeks old) male Swiss albino mice (Charles River, France) were isolated as previously described [ ]. .. Briefly, after pancreatic digestion with collagenase (Collagenase A; Boehringer Mannheim, Germany) in a stationary bath at 37°C, islets were separated by centrifugation and hand-picked under a stereomicroscope. ..

Article Title: Nutrient modulation of polarized and sustained submembrane Ca2+ microgradients in mouse pancreatic islet cells
Article Snippet: Islets from adult (8-10 weeks old) Swiss albino male mice (OF1), killed by cervical dislocation in accordance with national guidelines, were isolated as previously described ( ). .. Briefly, after pancreas digestion with collagenase (collagenase A, Boehringer Mannheim, Mannheim, Germany) in a stationary bath at 37°C, islets were separated by centrifugation and hand picked under a stereomicroscope. .. Once isolated, islets were dispersed into single cells by enzymatic digestion in the presence of 0.05 % trypsin plus 0.02 % EDTA for 3 min.

other:

Article Title: Voltage inactivation of Ca2+ entry and secretion associated with N- and P/Q-type but not L-type Ca2+ channels of bovine chromaffin cells
Article Snippet: Clostridium histolyticum collagenase (Boehringer-Mannheim); bovine serum albumin fraction V (Sigma).

Article Title: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging
Article Snippet: Collagenase D Boehringer Mannheim Lot #84612223

Incubation:

Article Title: A Quantitative Morphometric Analysis of Rat Spinal Cord Remyelination Following Transplantation of Allogenic Schwann Cells
Article Snippet: Schwann cells from P7 neonatal rat sciatic nerves were dissociated by mechanical trituration following a 30-minute incubation with 2.5 mg/ml trypsin (Boehringer Manheim, Mannheim, Germany) in calcium-free complete saline solution (CSS) as described in . .. Adult sciatic nerves were desheathed, minced, incubated for 45 minutes in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY) with 10 mg/ml each collagenase A + collagenase D (Boehringer Mannheim), pelleted, incubated for an additional 15 minutes in CSS with 2.5 mg/ml trypsin, and triturated though a fire-polished siliconized pasture pipette. .. Schwann cells were washed twice with DMEM + 10% fetal bovine serum before plating, and an additional 3 times in DMEM only before transplantation.

Modification:

Article Title: A Quantitative Morphometric Analysis of Rat Spinal Cord Remyelination Following Transplantation of Allogenic Schwann Cells
Article Snippet: Schwann cells from P7 neonatal rat sciatic nerves were dissociated by mechanical trituration following a 30-minute incubation with 2.5 mg/ml trypsin (Boehringer Manheim, Mannheim, Germany) in calcium-free complete saline solution (CSS) as described in . .. Adult sciatic nerves were desheathed, minced, incubated for 45 minutes in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY) with 10 mg/ml each collagenase A + collagenase D (Boehringer Mannheim), pelleted, incubated for an additional 15 minutes in CSS with 2.5 mg/ml trypsin, and triturated though a fire-polished siliconized pasture pipette. .. Schwann cells were washed twice with DMEM + 10% fetal bovine serum before plating, and an additional 3 times in DMEM only before transplantation.

Transferring:

Article Title: A Quantitative Morphometric Analysis of Rat Spinal Cord Remyelination Following Transplantation of Allogenic Schwann Cells
Article Snippet: Schwann cells from P7 neonatal rat sciatic nerves were dissociated by mechanical trituration following a 30-minute incubation with 2.5 mg/ml trypsin (Boehringer Manheim, Mannheim, Germany) in calcium-free complete saline solution (CSS) as described in . .. Adult sciatic nerves were desheathed, minced, incubated for 45 minutes in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY) with 10 mg/ml each collagenase A + collagenase D (Boehringer Mannheim), pelleted, incubated for an additional 15 minutes in CSS with 2.5 mg/ml trypsin, and triturated though a fire-polished siliconized pasture pipette. .. Schwann cells were washed twice with DMEM + 10% fetal bovine serum before plating, and an additional 3 times in DMEM only before transplantation.

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    Boehringer Mannheim collagenase
    Collagenase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Boehringer Mannheim collagenase a
    Collagenase A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase a/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase a - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

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