collagenase type ii  (Worthington Biochemical)


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    Name:
    Collagenase Type 2
    Description:
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    Catalog Number:
    LS004174
    Price:
    35
    Source:
    Clostridium histolyticum
    Size:
    100 mg
    Cas Number:
    9001.12.1
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    Structured Review

    Worthington Biochemical collagenase type ii
    Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
    https://www.bioz.com/result/collagenase type ii/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type ii - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Activation of intervertebral disc cells by co-culture with notochordal cells, conditioned medium and hypoxia
    Article Snippet: .. Porcine and bovine NP tissues were digested by 0.19% pronase (Roche, Basel, Switzerland) for 1 hour and subsequent collagenase type 2 at a concentration of 32 U/mL in 25 mL of HG-DMEM supplemented with 10% FCS (Worthington, London, UK) overnight (~14 hours) at 37°C and standard culture conditions (5% CO2 and 100% humidity) [ , ]. .. Cell separation using micro sieves and cytometry using FACS Cells isolated from porcine NP tissues were separated with three cell sieves, i.e., cells > 20 μm, 8-20 μm and cells < 8 μm (CellMicroSieves, BioDesign Inc., New York, USA) [ ] with the goal to identify NPC and NC.

    Mouse Assay:

    Article Title: The kallikrein-kinin system is falling into pieces: bradykinin fragments are biological active peptidesAngiotensin-( - Structure-function studies of Tityus serrulatus Hypotensin-I (TsHpt-I): A new agonist of B(
    Article Snippet: Cells were maintained with DMEM-F12 cell media supplemented by 10% FBS and 1% of antibiotic/antimycotic solution at 37°C until usage. .. Mice ventricular adult cardiomyocytes were isolated by collagenase type-2 (Worthington), as previously shown ( ) and kept in Tyrode media (1.3 x 10−1 mol.L-1 NaCl; 5.4 x 10−3 mol.L-1 KCl; 2,5 x 10−2 mol.L-1 HEPES; 5 x 10−4 mol.L-1 MgCl2 ; 3.3 x 10−4 mol.L-1 NaH2 PO4 ; 1.8 x 10−3 mol.L-1 mM CaCl2 ; 2.2 x 10−2 mol.L-1 glucose) until usage. .. For NO quantification, cells were starved with Hank’s Balanced Salt Solution (HBSS) for 1 hour and then loaded with DAF-FM diacetate (Thermo-Fisher) at a final concentration of 5 x 10−6 mol.L-1 for 30 minutes.

    Article Title: Macrophages redirect phagocytosis by non-professional phagocytes and influence inflammation
    Article Snippet: .. For lung harvests, mice were perfused through the right ventricle with PBS and the lungs were carefully excised and placed in type 2 collagenase (Worthington Biochemical Corporation) dissolved in HBSS containing Ca2+ and Mg2+ . .. The lung homogenate was then passed through a 70μm nylon strainer, spun down and treated with red blood cell lysis buffer (Sigma-Aldrich) for 5 minutes.

    Isolation:

    Article Title: The kallikrein-kinin system is falling into pieces: bradykinin fragments are biological active peptidesAngiotensin-( - Structure-function studies of Tityus serrulatus Hypotensin-I (TsHpt-I): A new agonist of B(
    Article Snippet: Cells were maintained with DMEM-F12 cell media supplemented by 10% FBS and 1% of antibiotic/antimycotic solution at 37°C until usage. .. Mice ventricular adult cardiomyocytes were isolated by collagenase type-2 (Worthington), as previously shown ( ) and kept in Tyrode media (1.3 x 10−1 mol.L-1 NaCl; 5.4 x 10−3 mol.L-1 KCl; 2,5 x 10−2 mol.L-1 HEPES; 5 x 10−4 mol.L-1 MgCl2 ; 3.3 x 10−4 mol.L-1 NaH2 PO4 ; 1.8 x 10−3 mol.L-1 mM CaCl2 ; 2.2 x 10−2 mol.L-1 glucose) until usage. .. For NO quantification, cells were starved with Hank’s Balanced Salt Solution (HBSS) for 1 hour and then loaded with DAF-FM diacetate (Thermo-Fisher) at a final concentration of 5 x 10−6 mol.L-1 for 30 minutes.

    Activity Assay:

    Article Title: Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering
    Article Snippet: Articular Chondrocyte Isolation and Culture Human chondrocytes (hCh) (n = 6) were isolated from the femoral heads of patients (age 63 ± 5.8 years old) undergoing hip replacement surgery (Ethic votum was obtained from local committee of the University Kiel, Code: D558/19, Date: 5 December 2019). .. The cartilage tissue was dissected into small pieces and washed twice with phosphate buffer saline (PBS; Sigma-Aldrich, Buchs, Switzerland) for 15 min. Cartilage pieces were first digested with 0.1% pronase (Roche, Mannheim, Germany) for 2 h and then with type 2 collagenase (Worthington, Lakewood, NJ, USA) with an enzyme activity of 600 U/mL for 14 h. Digestion solutions were prepared in Dulbecco’s modified Eagle’s medium (DMEM; Biochrom, Berlin, Germany) and the digestions were carried out at 37 °C in an atmosphere of 5% CO2 . ..

    Modification:

    Article Title: Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering
    Article Snippet: Articular Chondrocyte Isolation and Culture Human chondrocytes (hCh) (n = 6) were isolated from the femoral heads of patients (age 63 ± 5.8 years old) undergoing hip replacement surgery (Ethic votum was obtained from local committee of the University Kiel, Code: D558/19, Date: 5 December 2019). .. The cartilage tissue was dissected into small pieces and washed twice with phosphate buffer saline (PBS; Sigma-Aldrich, Buchs, Switzerland) for 15 min. Cartilage pieces were first digested with 0.1% pronase (Roche, Mannheim, Germany) for 2 h and then with type 2 collagenase (Worthington, Lakewood, NJ, USA) with an enzyme activity of 600 U/mL for 14 h. Digestion solutions were prepared in Dulbecco’s modified Eagle’s medium (DMEM; Biochrom, Berlin, Germany) and the digestions were carried out at 37 °C in an atmosphere of 5% CO2 . ..

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  • 99
    Worthington Biochemical collagenase type 2
    Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and <t>type</t> 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (
    Collagenase Type 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type 2/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type 2 - by Bioz Stars, 2021-06
    99/100 stars
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    80
    Worthington Biochemical enzyme
    Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and <t>type</t> 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (
    Enzyme, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme/product/Worthington Biochemical
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme - by Bioz Stars, 2021-06
    80/100 stars
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    Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and type 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: SMAD3 Deficiency Promotes Inflammatory Aortic Aneurysms in Angiotensin II-Infused Mice Via Activation of iNOS

    doi: 10.1161/JAHA.113.000269

    Figure Lengend Snippet: Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and type 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (

    Article Snippet: Briefly, the whole aorta was dissected out from its origin at the left ventricle to the iliac bifurcation, denuded of periaortic fat, cut into small pieces (≈2‐mm segments), and incubated in 5 mL of Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (#16000036; Life Technologies) and 1.36 mg/mL of type II collagenase (#LS004174; Worthington Biochemical Corporation) in agitation at 115 rpm for 2 hours at 37°C.

    Techniques: Concentration Assay, Construct, Incubation, Derivative Assay, Generated, Mouse Assay, Expressing, Ex Vivo