collagenase type ii  (Thermo Fisher)


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    Name:
    Collagenase Type II powder
    Description:
    Collagenase is a protease that cleaves the bond between a neutral amino acid X and glycine in the sequence Pro X Glyc Pro which is found with high frequency in collagen Collagenase is unique among proteases in its ability to degrade the triple helical native collagen fibrils commonly found in connective tissues such as skin tendon blood vessels and bone Collagenase disaggregation is suitable for the culture of human tumors mouse kidney human adult and fetal brain and many other tissues including epithelium Collagenase is relatively gentle dissociates well at physiological temperature and pH and requires no mechanical agitation or special equipment Gibco Collagenase Type II is isolated from Clostridium histolyticum and packaged as a lyophilized non sterile powder for research use in cell or tissue dissociation and organ perfusions Gibco Collagenase Type II activity is guaranteed to be greater than 125 units mg Compared to other collagenase preparations Gibco Collagenase Type II has higher clostripain activity and is well suited for the digestion of heart bone thyroid cartilage and liver tissues
    Catalog Number:
    17101015
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher collagenase type ii
    Collagenase is a protease that cleaves the bond between a neutral amino acid X and glycine in the sequence Pro X Glyc Pro which is found with high frequency in collagen Collagenase is unique among proteases in its ability to degrade the triple helical native collagen fibrils commonly found in connective tissues such as skin tendon blood vessels and bone Collagenase disaggregation is suitable for the culture of human tumors mouse kidney human adult and fetal brain and many other tissues including epithelium Collagenase is relatively gentle dissociates well at physiological temperature and pH and requires no mechanical agitation or special equipment Gibco Collagenase Type II is isolated from Clostridium histolyticum and packaged as a lyophilized non sterile powder for research use in cell or tissue dissociation and organ perfusions Gibco Collagenase Type II activity is guaranteed to be greater than 125 units mg Compared to other collagenase preparations Gibco Collagenase Type II has higher clostripain activity and is well suited for the digestion of heart bone thyroid cartilage and liver tissues
    https://www.bioz.com/result/collagenase type ii/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase type ii - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Maternal HLA Panel Reactive Antibodies in Early Gestation Positively Correlates with Chronic Chorioamnionitis: Evidence in Support of the Chronic Nature of Maternal Anti-fetal Rejection
    Article Snippet: .. Flow cytometric analyses of HLA class I and class II PRA in maternal sera were performed using the FlowPRA®-I screening test (One Lambda, Canoga Park, CA, USA) and the FlowPRA®-II screening test (One Lambda), according to the manufacturer’s instructions. .. Class I or class II microbeads were mixed with 20 μl of serum, and incubated for 30 min at room temperature with gentle rotation.

    Article Title: Characterization of the fetal blood transcriptome and proteome in maternal anti-fetal rejection: evidence of a distinct and novel type of human fetal systemic inflammatory response
    Article Snippet: .. Flow cytometric analyses of HLA class I and class II PRA in maternal sera were conducted using the FlowPRA®-I Screening Test and the FlowPRA®-II Screening Test (One Lambda, Inc., Canoga Park, CA, USA), according to the manufacturer’s instructions. .. HLA class I or class II microbeads were mixed with 20 μL of serum, followed by incubation for 30 min at room temperature with gentle rotation.

    Modification:

    Article Title: Antibody affinity and valency impact brain uptake of transferrin receptor-targeted gold nanoparticles
    Article Snippet: DNase I (Cat. No. 1014159001), collagenase/dispase (Cat. No. 109113), basic fibroblast growth factor (bFGF; Cat. No. 1363697), and insulin-transferrin-sodium selenite (Cat. No. 11074547001) were purchased from Roche (Hvidovre, DK). .. Pierce Traut's reagent (2-iminothiolane; Cat. No. 26101), nitrocellulose blotting membranes (Pre-cut sheets, 80×80 mm, 0.2 µm; Cat. No. LC2000) and 20X TBS buffer (Cat. No. 28358), rabbit anti-ZO-1 (Cat. No. 61-7300), Alexa Fluor 594-conjugated goat anti-mouse IgG (Cat. No. A11032), Alexa Fluor 594-conjugated goat anti-rabbit IgG (Cat. No. ), Alexa Fluor 488-conjugated donkey anti-rat IgG (Cat. No. A-21202), human IgG isotype control (Cat. No. 02-7102), collagenase II (Cat. No. 17101105), Dulbecco's Modified Eagle Medium (DMEM; Cat. No. 21885), DMEM-F12 GlutaMAXTM (DMEM-F12; Cat. No. 31331), fetal calf serum (Cat. No. 10270), and Mouse Aβ40 ELISA kit (Cat. No. KMB3481) were purchased from Thermo Scientific (Hvidovre, DK). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Antibody affinity and valency impact brain uptake of transferrin receptor-targeted gold nanoparticles
    Article Snippet: DNase I (Cat. No. 1014159001), collagenase/dispase (Cat. No. 109113), basic fibroblast growth factor (bFGF; Cat. No. 1363697), and insulin-transferrin-sodium selenite (Cat. No. 11074547001) were purchased from Roche (Hvidovre, DK). .. Pierce Traut's reagent (2-iminothiolane; Cat. No. 26101), nitrocellulose blotting membranes (Pre-cut sheets, 80×80 mm, 0.2 µm; Cat. No. LC2000) and 20X TBS buffer (Cat. No. 28358), rabbit anti-ZO-1 (Cat. No. 61-7300), Alexa Fluor 594-conjugated goat anti-mouse IgG (Cat. No. A11032), Alexa Fluor 594-conjugated goat anti-rabbit IgG (Cat. No. ), Alexa Fluor 488-conjugated donkey anti-rat IgG (Cat. No. A-21202), human IgG isotype control (Cat. No. 02-7102), collagenase II (Cat. No. 17101105), Dulbecco's Modified Eagle Medium (DMEM; Cat. No. 21885), DMEM-F12 GlutaMAXTM (DMEM-F12; Cat. No. 31331), fetal calf serum (Cat. No. 10270), and Mouse Aβ40 ELISA kit (Cat. No. KMB3481) were purchased from Thermo Scientific (Hvidovre, DK). ..

    Incubation:

    Article Title: Enhanced progenitor cell recruitment and endothelial repair after selective endothelial injury of the mouse kidney
    Article Snippet: Before analysis, renal tissue was placed in culture dishes and minced to small pieces (1–3 mm) immediately after washing in Hank's balanced salt solution (Mediatech, Herndon, VA). .. Collagenase type II (1 mg/ml; Invitrogen) was added and incubated at 37°C in 5% CO2 for 45 min. After digestion, the suspensions were filtered through 41 μm nylon mesh (Millipore). .. After being washed with PBS with 1% BSA, cells were incubated for 45 min on ice with FITC-conjugated anti-mouse CD34 (RAM34) (eBioscience) and PE-conjugated anti-mouse Flk-1 (Avas12α1) (BD Biosciences) or with FITC-conjugated anti-mouse CD117 (c-Kit) (BD Biosciences) and PE-conjugated anti-mouse CD150 (eBioscience).

    Amplification:

    Article Title: Crystal structures of an archaeal class II DNA photolyase and its complex with UV-damaged duplex DNA
    Article Snippet: Given the proven potential of class II photolyases to improve the UV resistance of plants, viruses and animals ( ; ; ), the structure-based engineering of these photolyases, for example, for the binding of different antenna chromophores, or the design of class-specific photolyase inhibitors is now a feasible route for future research. .. The Mm0852 gene encoding the class II photolyase of M. mazei Go1 (UniProt entry Q8PYK9_METMA) was amplified from genomic DNA with primers 5′-CTAGGC CATATG AATCCGAAACGTATCAGG-3′ and 5′-CCC CTCGAG CTACAAAGCTGAATATTTTTC-3′ (Invitrogen) introducing recognition sites for Nde I and Xho I (underlined). .. The PCR product was inserted between Nde I and Xho I sites of the vector pET-28a (Novagen) yielding an N-terminally His6 -tagged fusion protein.

    Isolation:

    Article Title: Definition of a Critical Size Osteochondral Knee Defect and its Negative Effect on the Surrounding Articular Cartilage in the Rat
    Article Snippet: Relative differences in expression were calculated using the 2−ΔΔCT method. .. Isolation and culture of rat chondrocytes Intact cartilage (non-operated positive control) from rat knee joints was dissected and digested with 0.2% type 2 collagenase (Thermo Fisher Scientific) in DMEM/F12 for 3 h at 37°C. ..

    Positive Control:

    Article Title: Definition of a Critical Size Osteochondral Knee Defect and its Negative Effect on the Surrounding Articular Cartilage in the Rat
    Article Snippet: Relative differences in expression were calculated using the 2−ΔΔCT method. .. Isolation and culture of rat chondrocytes Intact cartilage (non-operated positive control) from rat knee joints was dissected and digested with 0.2% type 2 collagenase (Thermo Fisher Scientific) in DMEM/F12 for 3 h at 37°C. ..

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  • 99
    Thermo Fisher gene exp hdac2 hs00231032 m1
    in silico Oxidative Stress and Antioxidant Defense Gene Network Interactions with Class I HDACs. Non-specific interactions between A) antioxidant, B) ROS metabolism genes, C) all class I HDACs. Tissue-specific interactions between D) HDAC1, <t>HDAC2</t> and HDAC3 with PCR array gene targets (CSDE1, CYBA, SGK2, TXNDC2) and individual HDACs interactions including E) HDAC1, F) HDAC2, and G) HDAC3. Interactions are color-coded by down-regulation, up-regulation, regulation, co-expression, chemical modification, physical interaction, predicted protein interaction, and predicted TFactor regulation.
    Gene Exp Hdac2 Hs00231032 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp hdac2 hs00231032 m1/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99/100 stars
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    85
    Thermo Fisher gene exp fgf2 ss03375809 u1
    The expression levels of COL1A1 and COL3A1 in bone marrow-derived multipotent mesenchymal stromal cells (MSCs) and periosteum-derived MSCs were significantly higher than those in MSCs from other tissues. The expression levels of <t>FGF2,</t> VEGFA, ICAM-1, and TIE-1 in gingiva-derived MSCs were significantly higher than those in MSCs from other tissues. Periosteum-derived MSCs showed the highest levels of TGF-β1 and ANG-1 expression of MSCs from all tissue types.
    Gene Exp Fgf2 Ss03375809 U1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp fgf2 ss03375809 u1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
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    93
    Thermo Fisher biotin anti mhc ii
    Expression of <t>MHC-II</t> on <t>CD11c</t> + BAL cells is restored by administration of rIL-12. Flow cytometric analysis of MHC-II + cells versus autofluorescence of gated CD11c + BAL cells recovered on (A) day 0 from WT and CD154 −/−
    Biotin Anti Mhc Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin anti mhc ii/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
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    Image Search Results


    in silico Oxidative Stress and Antioxidant Defense Gene Network Interactions with Class I HDACs. Non-specific interactions between A) antioxidant, B) ROS metabolism genes, C) all class I HDACs. Tissue-specific interactions between D) HDAC1, HDAC2 and HDAC3 with PCR array gene targets (CSDE1, CYBA, SGK2, TXNDC2) and individual HDACs interactions including E) HDAC1, F) HDAC2, and G) HDAC3. Interactions are color-coded by down-regulation, up-regulation, regulation, co-expression, chemical modification, physical interaction, predicted protein interaction, and predicted TFactor regulation.

    Journal: PLoS ONE

    Article Title: Profile of Class I Histone Deacetylases (HDAC) by Human Dendritic Cells after Alcohol Consumption and In Vitro Alcohol Treatment and Their Implication in Oxidative Stress: Role of HDAC Inhibitors Trichostatin A and Mocetinostat

    doi: 10.1371/journal.pone.0156421

    Figure Lengend Snippet: in silico Oxidative Stress and Antioxidant Defense Gene Network Interactions with Class I HDACs. Non-specific interactions between A) antioxidant, B) ROS metabolism genes, C) all class I HDACs. Tissue-specific interactions between D) HDAC1, HDAC2 and HDAC3 with PCR array gene targets (CSDE1, CYBA, SGK2, TXNDC2) and individual HDACs interactions including E) HDAC1, F) HDAC2, and G) HDAC3. Interactions are color-coded by down-regulation, up-regulation, regulation, co-expression, chemical modification, physical interaction, predicted protein interaction, and predicted TFactor regulation.

    Article Snippet: RNA was extracted and reverse transcribed, followed by qRT- PCR using Taqman assays (Applied Biosystems) for HDAC 1 (Hs02621185_s1), HDAC 2 (Hs00231032_m1), HDAC 3 ( Hs00187320_m1 ), and HDAC 8 (Hs00218503_m1).

    Techniques: In Silico, Polymerase Chain Reaction, Expressing, Modification

    BRD4 is necessary for the increase in BDNF mRNA following knock-down of HDAC2 and HDAC3. A , siRNA-mediated knock-down of class I/IIb HDACs and BRD4 (48 h) in HEK293 cells. B , BDNF mRNA expression following knock-down of class I/IIb HDACs in HEK-293 cells. C , Expression of BDNF mRNA following siRNA-mediated knock-down of HDAC2/BRD4, HDAC3/BRD4, or BRD4 in HEK-293 cells. D , siRNA-mediated knock-down of HDAC2, HDAC3, and BRD4 (48 h) in SH-SY5Y cells. E , BDNF mRNA expression following HDAC2, HDAC3 knock-down compared with control siRNA in SH-SY5Y cells. F , Expression of BDNF mRNA following siRNA-mediated knock-down of HDAC2/BRD4, HDAC3/BRD4, or BRD4 in SH-SY5Y cells. * p

    Journal: The Journal of Neuroscience

    Article Title: Enhancement of BDNF Expression and Memory by HDAC Inhibition Requires BET Bromodomain Reader Proteins

    doi: 10.1523/JNEUROSCI.1604-18.2018

    Figure Lengend Snippet: BRD4 is necessary for the increase in BDNF mRNA following knock-down of HDAC2 and HDAC3. A , siRNA-mediated knock-down of class I/IIb HDACs and BRD4 (48 h) in HEK293 cells. B , BDNF mRNA expression following knock-down of class I/IIb HDACs in HEK-293 cells. C , Expression of BDNF mRNA following siRNA-mediated knock-down of HDAC2/BRD4, HDAC3/BRD4, or BRD4 in HEK-293 cells. D , siRNA-mediated knock-down of HDAC2, HDAC3, and BRD4 (48 h) in SH-SY5Y cells. E , BDNF mRNA expression following HDAC2, HDAC3 knock-down compared with control siRNA in SH-SY5Y cells. F , Expression of BDNF mRNA following siRNA-mediated knock-down of HDAC2/BRD4, HDAC3/BRD4, or BRD4 in SH-SY5Y cells. * p

    Article Snippet: Using validated TaqMan primer probes for BDNF (Thermo Scientific, Hs03298540_m1, Rn02531967_s1, or Mm04230607_s1), HDAC1 (Hs02621185_s1), HDAC2 (Hs00231032_m1), HDAC3 (Hs00187320_m1), HDAC6 (Hs00997427_m1), HDAC8 (Hs00954353_g1), and HDAC10 (Hs00368899_m1), cDNA was run in triplicate and analyzed using the 2−ΔΔCT method with β-actin (Hs01060665_g, Rn00667869_m1, or Mm02619580_g1) or GAPDH (Hs02786624_g1, Rn01775763_g1, or Mm99999915_g1) as a normalization control.

    Techniques: Expressing

    The expression levels of COL1A1 and COL3A1 in bone marrow-derived multipotent mesenchymal stromal cells (MSCs) and periosteum-derived MSCs were significantly higher than those in MSCs from other tissues. The expression levels of FGF2, VEGFA, ICAM-1, and TIE-1 in gingiva-derived MSCs were significantly higher than those in MSCs from other tissues. Periosteum-derived MSCs showed the highest levels of TGF-β1 and ANG-1 expression of MSCs from all tissue types.

    Journal: Regenerative Therapy

    Article Title: Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation

    doi: 10.1016/j.reth.2017.02.001

    Figure Lengend Snippet: The expression levels of COL1A1 and COL3A1 in bone marrow-derived multipotent mesenchymal stromal cells (MSCs) and periosteum-derived MSCs were significantly higher than those in MSCs from other tissues. The expression levels of FGF2, VEGFA, ICAM-1, and TIE-1 in gingiva-derived MSCs were significantly higher than those in MSCs from other tissues. Periosteum-derived MSCs showed the highest levels of TGF-β1 and ANG-1 expression of MSCs from all tissue types.

    Article Snippet: Quantitative real-time PCR (StepOnePlus System, Applied Biosystems, Foster City, CA, USA) was performed using primers and probes (TaqMan Gene Expression Assays, Applied Biosystems) specific for collagen 1A1 (COL1A1) (Ss03373340_m1), collagen 3A1 (COL3A1) (Ss04323790_m1), VEGFA (Ss03393990), fibroblast growth factor 2 (FGF2) (Ss03375809_u1), transforming growth factor-β1 (TGF-β1) (Ss03382325_u1), angiopoietin-1 (ANG-1) (Ss03380513), intercellular adhesion molecule-1 (ICAM-1) (Ss033923), tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domains 1 (TIE-1) (Ss03373354), and β-actin (Ss03376081_u1).

    Techniques: Expressing, Derivative Assay

    Expression of MHC-II on CD11c + BAL cells is restored by administration of rIL-12. Flow cytometric analysis of MHC-II + cells versus autofluorescence of gated CD11c + BAL cells recovered on (A) day 0 from WT and CD154 −/−

    Journal:

    Article Title: In the Absence of CD154, Administration of Interleukin-12 Restores Th1 Responses but Not Protective Immunity to Schistosoma mansoni ▿

    doi: 10.1128/IAI.00252-07

    Figure Lengend Snippet: Expression of MHC-II on CD11c + BAL cells is restored by administration of rIL-12. Flow cytometric analysis of MHC-II + cells versus autofluorescence of gated CD11c + BAL cells recovered on (A) day 0 from WT and CD154 −/−

    Article Snippet: BAL cells (1 × 105 to 2 × 105 ) were blocked with 4 μl normal rabbit serum and then labeled with the following antibodies: fluorescein isothiocyanate anti-CD11c (clone HL3; Pharmingen), biotin anti-CD4 (clone H129.19; Pharmingen), and biotin anti-MHC-II (I-Ab,d clone 28-16-8S; Caltag).

    Techniques: Expressing, Flow Cytometry