Structured Review

Millipore collagenase type i
Collagenase Type I, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase type i/product/Millipore
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
collagenase type i - by Bioz Stars, 2021-04
97/100 stars

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Related Articles

Inhibition:

Article Title: The bioactivity of Hedysarum coronarium extracts on skin enzymes and cells correlates with phenolic content
Article Snippet: .. Collagenase (EC 3.4.24.3) inhibition was evaluated in a reaction mixture containing 50 mM tricine, pH 7.5, 10 mM CaCl2 , 400 mM NaCl, 0.8 mM FALGPA (Sigma-Aldrich, F5135) as the enzyme substrate, 0.16 units/mL collagenase from Clostridium histolyticum (Sigma-Aldrich, C0130) and serial water dilutions of extract stock solutions in DMSO, as indicated. ..

Injection:

Article Title: Estrogen and mechanical loading-related regulation of estrogen receptor-β and apoptosis in tendinopathy
Article Snippet: Using real-time ultrasonic guidance, the needle was placed parallel to the long axis of the transducer to confirm that the injection was peritendinous. .. To induce tendinopathy, the rats’ right Achilles tendons were intratendinously injected (ultrasonography-guided) with collagenase I (0.015 mg/ml) (Sigma-Aldrich, St. Louis, MO) to induce tendinopathy. .. The contralateral left leg was injected with phosphate-buffered saline (PBS).

Dissection:

Article Title: Isolation and Characterization of Neural Crest-Derived Stem Cells From Adult Ovine Palatal Tissue
Article Snippet: Additional control palatal tissue was extracted post-mortem at the Centre for Dairy Research (The University of Reading, Hall Farm) according to local guidelines. .. After mechanical dissection using a scalpel and surgical scissors, the palatal tissue was dissociated using 4 mg/ml Dispase (Sigma-Aldrich) at 4°C for overnight followed by Collagenase I (Sigma-Aldrich; 0.3 units/ml, 90 min, 37°C) treatment and mechanical trituration with a fire-polish glass Pasteur pipette. oNCSCs were cultured in serum-free media (Dulbeccos modified Eagles medium [DMEM]/F12; Sigma-Aldrich) containing basic FGF-2 (20 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany), EGF (20 ng/ml; Peprotech, London, United Kingdom), B27 supplement (Thermo-Fisher, Paisley, United Kingdom) in low adhesion T25 cell culture flasks (Sarstedt) in a humidified incubator at 37°C and 10% CO2 . .. After 8–10 days, the cultures reached sub-confluency and primary neurospheres were dissociated using Trypsin/EDTA (Sigma-Aldrich) and a 100 μm cell strainer (BD Falcon, Swindon, United Kingdom) resulting in an average of 2 × 106 single oNCSC per flask.

Transferring:

Article Title: Isolation and Characterization of Neural Crest-Derived Stem Cells From Adult Ovine Palatal Tissue
Article Snippet: Additional control palatal tissue was extracted post-mortem at the Centre for Dairy Research (The University of Reading, Hall Farm) according to local guidelines. .. After mechanical dissection using a scalpel and surgical scissors, the palatal tissue was dissociated using 4 mg/ml Dispase (Sigma-Aldrich) at 4°C for overnight followed by Collagenase I (Sigma-Aldrich; 0.3 units/ml, 90 min, 37°C) treatment and mechanical trituration with a fire-polish glass Pasteur pipette. oNCSCs were cultured in serum-free media (Dulbeccos modified Eagles medium [DMEM]/F12; Sigma-Aldrich) containing basic FGF-2 (20 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany), EGF (20 ng/ml; Peprotech, London, United Kingdom), B27 supplement (Thermo-Fisher, Paisley, United Kingdom) in low adhesion T25 cell culture flasks (Sarstedt) in a humidified incubator at 37°C and 10% CO2 . .. After 8–10 days, the cultures reached sub-confluency and primary neurospheres were dissociated using Trypsin/EDTA (Sigma-Aldrich) and a 100 μm cell strainer (BD Falcon, Swindon, United Kingdom) resulting in an average of 2 × 106 single oNCSC per flask.

Cell Culture:

Article Title: Isolation and Characterization of Neural Crest-Derived Stem Cells From Adult Ovine Palatal Tissue
Article Snippet: Additional control palatal tissue was extracted post-mortem at the Centre for Dairy Research (The University of Reading, Hall Farm) according to local guidelines. .. After mechanical dissection using a scalpel and surgical scissors, the palatal tissue was dissociated using 4 mg/ml Dispase (Sigma-Aldrich) at 4°C for overnight followed by Collagenase I (Sigma-Aldrich; 0.3 units/ml, 90 min, 37°C) treatment and mechanical trituration with a fire-polish glass Pasteur pipette. oNCSCs were cultured in serum-free media (Dulbeccos modified Eagles medium [DMEM]/F12; Sigma-Aldrich) containing basic FGF-2 (20 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany), EGF (20 ng/ml; Peprotech, London, United Kingdom), B27 supplement (Thermo-Fisher, Paisley, United Kingdom) in low adhesion T25 cell culture flasks (Sarstedt) in a humidified incubator at 37°C and 10% CO2 . .. After 8–10 days, the cultures reached sub-confluency and primary neurospheres were dissociated using Trypsin/EDTA (Sigma-Aldrich) and a 100 μm cell strainer (BD Falcon, Swindon, United Kingdom) resulting in an average of 2 × 106 single oNCSC per flask.

Modification:

Article Title: Isolation and Characterization of Neural Crest-Derived Stem Cells From Adult Ovine Palatal Tissue
Article Snippet: Additional control palatal tissue was extracted post-mortem at the Centre for Dairy Research (The University of Reading, Hall Farm) according to local guidelines. .. After mechanical dissection using a scalpel and surgical scissors, the palatal tissue was dissociated using 4 mg/ml Dispase (Sigma-Aldrich) at 4°C for overnight followed by Collagenase I (Sigma-Aldrich; 0.3 units/ml, 90 min, 37°C) treatment and mechanical trituration with a fire-polish glass Pasteur pipette. oNCSCs were cultured in serum-free media (Dulbeccos modified Eagles medium [DMEM]/F12; Sigma-Aldrich) containing basic FGF-2 (20 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany), EGF (20 ng/ml; Peprotech, London, United Kingdom), B27 supplement (Thermo-Fisher, Paisley, United Kingdom) in low adhesion T25 cell culture flasks (Sarstedt) in a humidified incubator at 37°C and 10% CO2 . .. After 8–10 days, the cultures reached sub-confluency and primary neurospheres were dissociated using Trypsin/EDTA (Sigma-Aldrich) and a 100 μm cell strainer (BD Falcon, Swindon, United Kingdom) resulting in an average of 2 × 106 single oNCSC per flask.

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  • 93
    Millipore rabbit anti na k atpase α2 subunit
    Schematic drawing of the ciliary body ( A , B ) and iris ( C , D ) showing the distribution of AQP1, AQP4, and Na + /K + <t>ATPase</t> in WKY and SHR rats. Ach: anterior chamber; Bv: blood vessel; ChA: chamber angle; Dm: dilator muscle; NPE: non-pigmented epithelium; PCh: Posterior chamber; PE: pigmented epithelium; PD: pupillary border; Sm: sphincter muscle; St: stroma.
    Rabbit Anti Na K Atpase α2 Subunit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti na k atpase α2 subunit/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti na k atpase α2 subunit - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    97
    Millipore collagen i
    DIAPH3 knockdown induces an amoeboid phenotype in transformed cells Morphology of DIAPH3-depleted HMEC, compared to control cells. Plastic (2D), basement membrane cultures (3D). Scale = 200 µm. HRAS V12 cells grown in 2D adopt a round, refractile appearance when DIAPH3 is silenced. Scale = 100 µm. HMEC-HRAS V12 cells form cell aggregates with cord-like protrusions in 3D. DIAPH3 depletion causes junctional instability, with migration of single cells into the surrounding matrix ( inset , right ). Scale = 200 µm. Representative trajectories of control (green) and DIAPH3-deficient (red) HMEC-HRAS V12 cells. Quantification of migration speed, determined from C . DIAPH3-silenced HRAS V12 -HMEC display increased invasiveness. Growth of DIAPH3-deficient and control DU145 cells on a thick layer of collagen I. Arrowheads mark cell surface blebbing (top). Visualization of the cytoskeleton with phalloidin by immunofluorescence (IF) shows prominent cortical actin in DIAPH3-deficient cells (arrowhead, bottom). Scale = 50 µm. Increase in amoeboid morphology following expression of DIAPH3 siRNA. Invasion of <t>collagen</t> I by DU145 cells, with FBS or EGF as chemo-attractants, is increased in DIAPH3-deficient cells ( N ≥ 2 independent trials). See also Supporting Information Movies S1 and S2.
    Collagen I, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagen i/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagen i - by Bioz Stars, 2021-04
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    95
    Millipore mouse anti collagen type ii
    Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with <t>anti-adiponectin</t> (A, B) , anti-osteocalcin (C, D) , <t>anti-collagen</t> <t>type</t> <t>II</t> (E, F) , respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo . The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D) . More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II ( F , red). (P
    Mouse Anti Collagen Type Ii, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti collagen type ii/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Millipore decorin
    Adhesion, morphology and phenotypic functions of primary rat hepatocytes on polyelectrolyte multilayers (PEMs) modified with extracellular matrix proteins. (A) Quantification of hepatocyte adhesion on substrates modified with either type I collagen (100 μg/mL) or collagen mixed with the proteoglycan <t>decorin</t> (25 μg/mL). All data are normalized to hepatocyte adhesion on collagen-coated TCPS. Error bars are SEM ( n = 6–8). Pairwise differences among collagen-modified substrates of varying compliance were not statistically significant (n.s.). # p
    Decorin, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/decorin/product/Millipore
    Average 93 stars, based on 1 article reviews
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    decorin - by Bioz Stars, 2021-04
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    Image Search Results


    Schematic drawing of the ciliary body ( A , B ) and iris ( C , D ) showing the distribution of AQP1, AQP4, and Na + /K + ATPase in WKY and SHR rats. Ach: anterior chamber; Bv: blood vessel; ChA: chamber angle; Dm: dilator muscle; NPE: non-pigmented epithelium; PCh: Posterior chamber; PE: pigmented epithelium; PD: pupillary border; Sm: sphincter muscle; St: stroma.

    Journal: Cells

    Article Title: Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na+, K+ ATPase in Hypertensive Rats

    doi: 10.3390/cells7110210

    Figure Lengend Snippet: Schematic drawing of the ciliary body ( A , B ) and iris ( C , D ) showing the distribution of AQP1, AQP4, and Na + /K + ATPase in WKY and SHR rats. Ach: anterior chamber; Bv: blood vessel; ChA: chamber angle; Dm: dilator muscle; NPE: non-pigmented epithelium; PCh: Posterior chamber; PE: pigmented epithelium; PD: pupillary border; Sm: sphincter muscle; St: stroma.

    Article Snippet: All sections were incubated overnight at 4 °C with an appropriate primary antibody: rabbit anti-AQP4 (ab2218 Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, 1:2500), mouse anti-AQP1 (Ab9566 Abcam, Canbridge, UK 1:1000), mouse anti-Na+ /K+ ATPase α1-subunit (Ab 7671 Abcam, 1:400), and rabbit anti-Na+ /K+ ATPase α2-subunit (07-674 Millipore, Burlington, MA, USA, 1:400).

    Techniques:

    Confocal microscopy images of the expression of Na + /K + ATPase α1 and α2 subunits in the ciliary body of WKY ( A ) rats and SHR ( B ) rats. The Na + /K + ATPase α1 subunit is located in the basal membrane of the PE of the CB and the staining was higher in SHR when compared to WKY rats ( C , F ). A decrease in the expression of α2 in SHR ( D , G ) rats was observed in the basal membrane of the NPE of the ciliary body. Images ( E , H ) show the localization of both subunits in the CB. At the bottom, values of stain intensities in r.u. for Na + /K + ATPase α1 ( I ) and Na + /K + ATPase α1 ( J ) are represented as the means ± SD ( n = ten animals per group) * p

    Journal: Cells

    Article Title: Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na+, K+ ATPase in Hypertensive Rats

    doi: 10.3390/cells7110210

    Figure Lengend Snippet: Confocal microscopy images of the expression of Na + /K + ATPase α1 and α2 subunits in the ciliary body of WKY ( A ) rats and SHR ( B ) rats. The Na + /K + ATPase α1 subunit is located in the basal membrane of the PE of the CB and the staining was higher in SHR when compared to WKY rats ( C , F ). A decrease in the expression of α2 in SHR ( D , G ) rats was observed in the basal membrane of the NPE of the ciliary body. Images ( E , H ) show the localization of both subunits in the CB. At the bottom, values of stain intensities in r.u. for Na + /K + ATPase α1 ( I ) and Na + /K + ATPase α1 ( J ) are represented as the means ± SD ( n = ten animals per group) * p

    Article Snippet: All sections were incubated overnight at 4 °C with an appropriate primary antibody: rabbit anti-AQP4 (ab2218 Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, 1:2500), mouse anti-AQP1 (Ab9566 Abcam, Canbridge, UK 1:1000), mouse anti-Na+ /K+ ATPase α1-subunit (Ab 7671 Abcam, 1:400), and rabbit anti-Na+ /K+ ATPase α2-subunit (07-674 Millipore, Burlington, MA, USA, 1:400).

    Techniques: Confocal Microscopy, Expressing, Staining

    DIAPH3 knockdown induces an amoeboid phenotype in transformed cells Morphology of DIAPH3-depleted HMEC, compared to control cells. Plastic (2D), basement membrane cultures (3D). Scale = 200 µm. HRAS V12 cells grown in 2D adopt a round, refractile appearance when DIAPH3 is silenced. Scale = 100 µm. HMEC-HRAS V12 cells form cell aggregates with cord-like protrusions in 3D. DIAPH3 depletion causes junctional instability, with migration of single cells into the surrounding matrix ( inset , right ). Scale = 200 µm. Representative trajectories of control (green) and DIAPH3-deficient (red) HMEC-HRAS V12 cells. Quantification of migration speed, determined from C . DIAPH3-silenced HRAS V12 -HMEC display increased invasiveness. Growth of DIAPH3-deficient and control DU145 cells on a thick layer of collagen I. Arrowheads mark cell surface blebbing (top). Visualization of the cytoskeleton with phalloidin by immunofluorescence (IF) shows prominent cortical actin in DIAPH3-deficient cells (arrowhead, bottom). Scale = 50 µm. Increase in amoeboid morphology following expression of DIAPH3 siRNA. Invasion of collagen I by DU145 cells, with FBS or EGF as chemo-attractants, is increased in DIAPH3-deficient cells ( N ≥ 2 independent trials). See also Supporting Information Movies S1 and S2.

    Journal: EMBO Molecular Medicine

    Article Title: DIAPH3 governs the cellular transition to the amoeboid tumour phenotype

    doi: 10.1002/emmm.201200242

    Figure Lengend Snippet: DIAPH3 knockdown induces an amoeboid phenotype in transformed cells Morphology of DIAPH3-depleted HMEC, compared to control cells. Plastic (2D), basement membrane cultures (3D). Scale = 200 µm. HRAS V12 cells grown in 2D adopt a round, refractile appearance when DIAPH3 is silenced. Scale = 100 µm. HMEC-HRAS V12 cells form cell aggregates with cord-like protrusions in 3D. DIAPH3 depletion causes junctional instability, with migration of single cells into the surrounding matrix ( inset , right ). Scale = 200 µm. Representative trajectories of control (green) and DIAPH3-deficient (red) HMEC-HRAS V12 cells. Quantification of migration speed, determined from C . DIAPH3-silenced HRAS V12 -HMEC display increased invasiveness. Growth of DIAPH3-deficient and control DU145 cells on a thick layer of collagen I. Arrowheads mark cell surface blebbing (top). Visualization of the cytoskeleton with phalloidin by immunofluorescence (IF) shows prominent cortical actin in DIAPH3-deficient cells (arrowhead, bottom). Scale = 50 µm. Increase in amoeboid morphology following expression of DIAPH3 siRNA. Invasion of collagen I by DU145 cells, with FBS or EGF as chemo-attractants, is increased in DIAPH3-deficient cells ( N ≥ 2 independent trials). See also Supporting Information Movies S1 and S2.

    Article Snippet: Where indicated, cells plated on collagen I were pretreated with nocodazole (2 µM, Sigma) for 30 min, and processed as above with EGFR antibodies.

    Techniques: Transformation Assay, Migration, Immunofluorescence, Expressing

    Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with anti-adiponectin (A, B) , anti-osteocalcin (C, D) , anti-collagen type II (E, F) , respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo . The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D) . More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II ( F , red). (P

    Journal: BMC Musculoskeletal Disorders

    Article Title: Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair

    doi: 10.1186/s12891-015-0511-8

    Figure Lengend Snippet: Immunocytochemistry staining on complexes implanted Matrigel with BMSCs or MMSCs into the back of nude rats for 3 weeks. The tissue sections were stained with anti-adiponectin (A, B) , anti-osteocalcin (C, D) , anti-collagen type II (E, F) , respectively. It is seen that two kinds of stem cells have multi-differentiation potential in vivo . The implantation of BMSCs resulted in more bone-like tissue formation (C, pink) than MMSCs (D) . More cartilage-like tissues were observed in MMSCs group, as shown by immunostaining for collagen type II ( F , red). (P

    Article Snippet: The tissue sections were fixed with 4% paraformaldehyde for 15 min and reacted with mouse anti-adiponectin (1:300, Millipore; Cat. #MAB3604; Temecula, CA), mouse anti-osteocalcin (1:200, Abcam, Cat #13418; Cambridge, MA), mouse anti-collagen type I (1:100, Millipore, Cat. #MAB1340; Temecula, CA), and mouse anti-collagen type II (1:100, Millipore, Cat. #MAB1330, Temecula, CA) at room temperature for 2 hours.

    Techniques: Immunocytochemistry, Staining, In Vivo, Immunostaining

    Adhesion, morphology and phenotypic functions of primary rat hepatocytes on polyelectrolyte multilayers (PEMs) modified with extracellular matrix proteins. (A) Quantification of hepatocyte adhesion on substrates modified with either type I collagen (100 μg/mL) or collagen mixed with the proteoglycan decorin (25 μg/mL). All data are normalized to hepatocyte adhesion on collagen-coated TCPS. Error bars are SEM ( n = 6–8). Pairwise differences among collagen-modified substrates of varying compliance were not statistically significant (n.s.). # p

    Journal: Biomaterials

    Article Title: Modulation of hepatocyte phenotype in vitro via chemomechanical tuning of polyelectrolyte multilayers

    doi: 10.1016/j.biomaterials.2008.10.055

    Figure Lengend Snippet: Adhesion, morphology and phenotypic functions of primary rat hepatocytes on polyelectrolyte multilayers (PEMs) modified with extracellular matrix proteins. (A) Quantification of hepatocyte adhesion on substrates modified with either type I collagen (100 μg/mL) or collagen mixed with the proteoglycan decorin (25 μg/mL). All data are normalized to hepatocyte adhesion on collagen-coated TCPS. Error bars are SEM ( n = 6–8). Pairwise differences among collagen-modified substrates of varying compliance were not statistically significant (n.s.). # p

    Article Snippet: Substrates were coated with 100 μg/mL collagen I or 100 μg/mL collagen I pre-mixed with 25 μg/mL decorin (Sigma) for 2–3 h at 37°C.

    Techniques: Modification