Article Title: An organoid model derived from human adipose stem/progenitor cells to study adipose tissue physiology
Figure Lengend Snippet: ASCs derived from adipose organoids retain the capability to form colonies. (a) Colony formation assays by ASCs derived from collagenase I-digested organoids and proliferating ASCs directly isolated from WAT of given donors are shown. Colonies are stained with crystal violet and counted. A representative result of n = 3 different donors, mean ± SEM, is shown. (b) Number of colonies formed by SVF-derived proliferating ASCs and digested organoids among n = 3 different donors. Three wells or organoids were used for each condition and donor.
Article Snippet: Next, Collagenase I digestion (200 U/ml Collagenase I (CLS Type I, Worthington Biochemical Corp., Lakewood, NJ) in PBS supplemented with 2% w/v BSA) was performed under stirring for 1 h at 37°C.
Techniques: Derivative Assay, Isolation, Staining