collagenase type 2 (Worthington Biochemical)

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Collagenase Type 2

Description:

Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder

Catalog Number:

ls004174

Price:

Source:

Clostridium histolyticum

Size:

100 mg

Cas Number:

9001.12.1

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Structured Review

Worthington Biochemical collagenase type 2

Neonatal hyperoxia promotes AHR, mucus production and <t>type</t> 2 airway inflammation (A) Experimental schemes. Neonatal mice were exposed to hyperoxia or RA for 7 days and then housed in RA until PN28 for analysis of AHR, histology and lung inflammation. (B) Airway resistance (RL) was determined following methacholine challenge in mice exposed to RA or O2. n=5 or 6 mice per group (mean ± SEM). (C) Lung sections were stained with hematoxylin and eosin (H E) or periodic acid-Schiff (PAS). (D) Pathology scores on PAS staining sections were quantified. n=13 or 14 mice per group (mean ± SEM). (E) % of PAS-positive areas within airway epithelium was calculated by image analysis. n=13 or 14 mice per group (mean ± SEM). (F) Lung mucus associated genes were measured by quantitative RT-PCR. n=3 or 4 mice per group (mean ± SEM). (G) Lung cytokine gene expressions were determined by quantitative RT-PCR. n=6 mice per group (mean ± SEM). (H) The frequencies and absolute numbers of eosinophils (CD45+SiglecF+CD11c−) in BAL were measured by flow cytometry. n=3 mice per group (mean ± SEM). (I) The frequencies and absolute numbers of neutrophils (CD45+Ly6G+CD11b+) in BAL were measured by flow cytometry. n=3 mice per group (mean ± SEM). Data are representative of at least three independent experiments. n.s., not significant; * P ≤ 0.05.

Prepared to contain higher clostripain activity Suggested for bone heart liver thyroid and salivary primary cell isolation Supplied as a dialyzed lyophilized powder
https://www.bioz.com/result/collagenase type 2/product/Worthington Biochemical
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99

collagenase type 2 - by Bioz Stars, 2021-03

86/100 stars

Images

1) Product Images from "Neonatal hyperoxia promotes asthma-like features through IL-33-dependent ILC2 responses"

Article Title: Neonatal hyperoxia promotes asthma-like features through IL-33-dependent ILC2 responses

Journal: The Journal of allergy and clinical immunology

doi: 10.1016/j.jaci.2017.11.025

Neonatal hyperoxia promotes AHR, mucus production and type 2 airway inflammation (A) Experimental schemes. Neonatal mice were exposed to hyperoxia or RA for 7 days and then housed in RA until PN28 for analysis of AHR, histology and lung inflammation. (B) Airway resistance (RL) was determined following methacholine challenge in mice exposed to RA or O2. n=5 or 6 mice per group (mean ± SEM). (C) Lung sections were stained with hematoxylin and eosin (H E) or periodic acid-Schiff (PAS). (D) Pathology scores on PAS staining sections were quantified. n=13 or 14 mice per group (mean ± SEM). (E) % of PAS-positive areas within airway epithelium was calculated by image analysis. n=13 or 14 mice per group (mean ± SEM). (F) Lung mucus associated genes were measured by quantitative RT-PCR. n=3 or 4 mice per group (mean ± SEM). (G) Lung cytokine gene expressions were determined by quantitative RT-PCR. n=6 mice per group (mean ± SEM). (H) The frequencies and absolute numbers of eosinophils (CD45+SiglecF+CD11c−) in BAL were measured by flow cytometry. n=3 mice per group (mean ± SEM). (I) The frequencies and absolute numbers of neutrophils (CD45+Ly6G+CD11b+) in BAL were measured by flow cytometry. n=3 mice per group (mean ± SEM). Data are representative of at least three independent experiments. n.s., not significant; * P ≤ 0.05.

Figure Legend Snippet: Neonatal hyperoxia promotes AHR, mucus production and type 2 airway inflammation (A) Experimental schemes. Neonatal mice were exposed to hyperoxia or RA for 7 days and then housed in RA until PN28 for analysis of AHR, histology and lung inflammation. (B) Airway resistance (RL) was determined following methacholine challenge in mice exposed to RA or O2. n=5 or 6 mice per group (mean ± SEM). (C) Lung sections were stained with hematoxylin and eosin (H E) or periodic acid-Schiff (PAS). (D) Pathology scores on PAS staining sections were quantified. n=13 or 14 mice per group (mean ± SEM). (E) % of PAS-positive areas within airway epithelium was calculated by image analysis. n=13 or 14 mice per group (mean ± SEM). (F) Lung mucus associated genes were measured by quantitative RT-PCR. n=3 or 4 mice per group (mean ± SEM). (G) Lung cytokine gene expressions were determined by quantitative RT-PCR. n=6 mice per group (mean ± SEM). (H) The frequencies and absolute numbers of eosinophils (CD45+SiglecF+CD11c−) in BAL were measured by flow cytometry. n=3 mice per group (mean ± SEM). (I) The frequencies and absolute numbers of neutrophils (CD45+Ly6G+CD11b+) in BAL were measured by flow cytometry. n=3 mice per group (mean ± SEM). Data are representative of at least three independent experiments. n.s., not significant; * P ≤ 0.05.

Techniques Used: Mouse Assay, Staining, Quantitative RT-PCR, Flow Cytometry, Cytometry

In Vitro:

Article Title: Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering
Article Snippet: .. 2.5 In vitro Enzymatic Degradation of dMEP NanofibersTo explore the degradation behavior of dMEP scaffolds, acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C, and then digested in 100 ug/mL proteinase K in tris-HCl overnight at 60°C. .. A scaffold from each group was used for SEM imaging to visualize changes in fiber morphology and structure after enzymatic degradation, and the remaining were used for the orthohydroxyproline (OHP) assay to quantify remaining collagen [ ].

FACS:

Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance
Article Snippet: Proteins were detected by Li-COR (Odyssey CLX) Western blot scanner, and densitometric analysis was performed using Image Studio v4.1. .. Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase). .. Stromal pellets were incubated in red blood cell lysis buffer (Sigma Aldrich), washed, and stained with fluorescent-conjugated antibodies for CD45, CD31, Sca1, CD29, CD34, and CD24 as described .

Incubation:

Article Title: Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells
Article Snippet: Skin samples were incubated in 2 mg/ml dispase (383-02281; Wako Pure Chemical Industries, Osaka, Japan) and were separated into epidermis and dermis. .. Dermis was minced and then incubated with 2 mg/ml collagenase type 2 (CLS-2; Worthington Biochemical, Lakewood, NJ, USA) in Tyrode’s solution for 60–90 minutes. ..

Mouse Assay:

Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart
Article Snippet: The protocol is based on that originally developed by the Alliance for Cell Signalling (AfCS) for adult mouse cardiomyocyte isolation , with some minor modifications. .. Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington). ..

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collagenase type 2 (Worthington Biochemical)

Worthington Biochemical collagenase type 2

Proposed model of endocrine therapy effects on adipose tissue. ERα signaling maintains adipocyte progenitor pools and inhibits preadipocyte expansion, adipocyte differentiation, and hypertrophy. Disruption of E 2 signaling through either tamoxifen treatment or withdrawal of E 2 depletes the adipocyte progenitor pool causing adipocyte hypertrophy consistent with a phenotype that precedes insulin resistance and the development of <t>type</t> 2 diabetes.

Collagenase Type 2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase type 2/product/Worthington Biochemical
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

collagenase type 2 - by Bioz Stars, 2021-03

99/100 stars

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Image Search Results

$[A] Digestion of BAT with collagenase I produces an adipose fraction harboring brown adipocytes and a stromal fraction composed primarily of endothelium separated by a fluid portion. [B] Deep-red fluorescence intensity images of centrifugation tubes containing$

Journal: Journal of materials chemistry. B, Materials for biology and medicine

Article Title: Fluorescence Imaging of Interscapular Brown Adipose Tissue in Living Mice †

doi: 10.1039/C4TB01914H

Figure Lengend Snippet: [A] Digestion of BAT with collagenase I produces an adipose fraction harboring brown adipocytes and a stromal fraction composed primarily of endothelium separated by a fluid portion. [B] Deep-red fluorescence intensity images of centrifugation tubes containing

Article Snippet: The minced tissue was digested with 2 mg/ml Collagenase I (Worthington Biochemical Corporation) in Hanks' Balanced Salt Solution (HBSS) (1.3 mM CaCl2 , 0.5 mM MgCl2 •6H2 O, 0.5 mM MgSO4 •7H2 O, 5.3 mM KCl, 0.40 mM KH2 PO4 , 4.2 mM NaHCO3 , 140 mM, NaCl, 0.3 mM Na2 HPO4 , pH 7.5) at 37 °C for 2 hours.

Techniques: Fluorescence, Centrifugation

Journal: bioRxiv

Article Title: Breast cancer endocrine therapy exhausts adipocyte progenitors promoting weight gain and glucose intolerance

doi: 10.1101/2020.08.21.259440

Figure Lengend Snippet: Proposed model of endocrine therapy effects on adipose tissue. ERα signaling maintains adipocyte progenitor pools and inhibits preadipocyte expansion, adipocyte differentiation, and hypertrophy. Disruption of E 2 signaling through either tamoxifen treatment or withdrawal of E 2 depletes the adipocyte progenitor pool causing adipocyte hypertrophy consistent with a phenotype that precedes insulin resistance and the development of type 2 diabetes.

Article Snippet: Adipose Tissue FACS Whole inguinal subcutaneous adipose depots were excised, minced briefly (5 min) with dissecting scissors, and digested for 75 mins at 37C in a collagenase solution (Collagenase type II, Worthington LS004177). (HBSS with 3% BSA, 0.8mM ZnCl2, Mg, Ca, 0.8mg/ml collagenase).

Techniques:

Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and type 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: SMAD3 Deficiency Promotes Inflammatory Aortic Aneurysms in Angiotensin II-Infused Mice Via Activation of iNOS

doi: 10.1161/JAHA.113.000269

Figure Lengend Snippet: Defective contractile function of S3KO aorta. A through C, PE‐induced contraction of WT and S3KO aortic rings treated with placebo (A), 200 μmol/L l‐NAME (B), or 200 μmol/L aminoguanidine (C) (n=4 per group; duplicate experiments were performed). Contraction was measured at PE concentrations ranging from 10 −10 to 10 −3 mol/L, and concentration–response curves were constructed. Note that contraction is expressed as the percentage of maximal contraction induced by incubation of aortic rings in 80 mmol/L high potassium (Hi‐K + ) buffer. For placebo and l‐NAME treatment, endothelium‐intact aortic segments were used, whereas endothelium‐denuded aortas were used in aminoguanidine treatment to eliminate the vasorelaxant effect of eNOS‐derived NO. D, pEC 50 of WT and S3KO aortas in response to PE, indicating tissue sensitivity toward the drug. Note that pEC 50 is the negative logarithm of EC 50 , which refers to the concentration of a drug that induces a half‐maximal response. E, Maximal contraction force generated when incubated with 80 mmol/L Hi‐K + buffer. F, Aortic mRNA level of AngII type 1a (AT1a), type 1b (AT1b), and type 2 (AT2) receptors in WT and S3KO mice (n=3 per group; triplicate experiments were performed). Gene expression was normalized to RPL27. G through I, AngII‐induced contraction in ex vivo aortic segments treated with placebo (G), 200 μmol/L l‐NAME (H), or 200 μmol/L aminoguanidine (I) (n=5 per group; duplicate experiments were performed). Note that AngII elicited a rapid and transient contraction of infrarenal aortic segments at a concentration of 1 μg/mL, whereas minimal (

Article Snippet: Briefly, the whole aorta was dissected out from its origin at the left ventricle to the iliac bifurcation, denuded of periaortic fat, cut into small pieces (≈2‐mm segments), and incubated in 5 mL of Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (#16000036; Life Technologies) and 1.36 mg/mL of type II collagenase (#LS004174; Worthington Biochemical Corporation) in agitation at 115 rpm for 2 hours at 37°C.

Techniques: Concentration Assay, Construct, Incubation, Derivative Assay, Generated, Mouse Assay, Expressing, Ex Vivo

The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and type 2 diabetic ( db/db ) under basal conditions and in response

Journal: Life sciences

Article Title: Cardiac O-GlcNAcylation blunts autophagic signaling in the diabetic heart

doi: 10.1016/j.lfs.2012.06.011

Figure Lengend Snippet: The role of the hexosamine biosynthesis pathway (HBP) in blunting autophagic signaling. Representative western blots showing Beclin-1 and LC3 protein in cardiomyocytes from non-diabetic (Con) and type 2 diabetic ( db/db ) under basal conditions and in response

Article Snippet: Briefly, hearts were rapidly excised from heparinized and anesthetized mice, perfused retrogradely with Ca2+ -free perfusion buffer consisting of (in mM) 0.6 KH2 PO4 , 0.6 Na2 HPO4 , 1.2 MgSO4 • 7H2 O, 0.032 phenol red, 12 NaHCO3 , 10 KHCO3 , 10 HEPES, 30 taurine, 10 2,3-butanedione monoxime, and 5.5 glucose, pH 7.46, followed by buffer containing 12.5 μM CaCl2 and 0.4 mg/ml collagenase type 2 (Worthington).

Techniques: Western Blot

The temporal progression of body weight (A) and survival (B) following either sham or trans-aortic constriction surgery to induce a pressure overload (PO) in non-diabetic (control), high fat-fed, or high fat-fed and STZ treated type 2 diabetic rats. †