collagenase type 2  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher collagenase type 2
    Collagenase Type 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type 2/product/Thermo Fisher
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    collagenase type 2 - by Bioz Stars, 2020-05
    99/100 stars

    Images

    Related Articles

    Modification:

    Article Title: Effects of dopamine on leptin release and leptin gene (OB) expression in adipocytes from obese and hypertensive patients
    Article Snippet: .. Dulbecco’s Modified Eagle’s Medium (DMEM), bovine serum albumin (BSA), collagenase type II, PBS, penicillin and streptomycin, and cell-culture reagents were purchased from Life Technologies (Carlsbad, CA, USA). .. A Titan one-tube reverse-transcription polymerase chain reaction (RT-PCR) kit (Roche Diagnostics, Basel, Switzerland) was used RT and PCR.

    Isolation:

    Article Title: Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
    Article Snippet: .. Isolation of single cells from embryonic zebrafish Transgenic tg(myl7:GFP) 2-day-old embryos (n = 200) were dechorionated and digested in HBSS Ca2+ /Mg2+ free media containing 0.1% collagenase type II (Gibco) at 32°C for 30–40 min followed by 1X TrypLE Express (Gibco) for 15–30 min at 32°C with agitation. ..

    Incubation:

    Article Title: The Influence of Chronic Wound Extracts on Inflammatory Cytokine and Histatin Stability
    Article Snippet: .. The next day, epidermis was removed and discarded and the dermis was incubated in collagenase type II (Gibco, Life Technologies, Grand Island, USA)/dispase II in Hanks balanced salt solution (HBSS) (Gibco) at 37°C for 2–3 h. Fibroblast medium consisting of DMEM (Lonza, Verviers, Belgium) containing 1% ultroserG (UG) (Biosepra, Cergy-Saint-Christopher, France) and 1% penicillin-streptomycin (P/S) (Gibco) was added and the solution was passed through an 100 μm and 40 μm cell strainer (Becton Dickinson Falcon, Erembodegem, Belgium). .. A single cell suspension was seeded at ± 3.5 x 104 cells cm2 and further cultured in fibroblast medium.

    Cell Culture:

    Article Title: Effects of dopamine on leptin release and leptin gene (OB) expression in adipocytes from obese and hypertensive patients
    Article Snippet: .. Dulbecco’s Modified Eagle’s Medium (DMEM), bovine serum albumin (BSA), collagenase type II, PBS, penicillin and streptomycin, and cell-culture reagents were purchased from Life Technologies (Carlsbad, CA, USA). .. A Titan one-tube reverse-transcription polymerase chain reaction (RT-PCR) kit (Roche Diagnostics, Basel, Switzerland) was used RT and PCR.

    Transgenic Assay:

    Article Title: Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
    Article Snippet: .. Isolation of single cells from embryonic zebrafish Transgenic tg(myl7:GFP) 2-day-old embryos (n = 200) were dechorionated and digested in HBSS Ca2+ /Mg2+ free media containing 0.1% collagenase type II (Gibco) at 32°C for 30–40 min followed by 1X TrypLE Express (Gibco) for 15–30 min at 32°C with agitation. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher type 2 collagenase
    Instability of the newly formed cartilage tissue and subchondral bone plate migration in small size defects (A): Representative SafO staining and immunohistochemical staining for <t>type</t> 2 collagen and type 1 collagen of small osteochondral defects at 16 weeks. (Bar = 500 μm. Yellow dot line in 0.9 mm group indicates subchondral bone plate. Black dotted line indicated the original defect borders) (B): Quantification of proportion of SafO positivity in which 100% indicated same size as native cartilage in the small defect size group until 16 weeks. (C): Quantification of the relative proportion of the subchondral bone area in which 100% indicated same size as the drilling size in the 0.9 mm group until 16 weeks. (D): Quantification for areas of subchondral bone plate distance in which 0 mm indicated same depth as native subchondral bone plate depth in the 0.9 mm group until 16 weeks. ( n = 4, * P
    Type 2 Collagenase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type 2 collagenase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    type 2 collagenase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher matrix metalloproteinase 2
    Epithelial-mesenchymal transition in the present case. Immunohistochemistry for transcriptional factors, Slug (A) and Twist (B) . Nuclear staining for Slug and Twist was observed in the neoplastic cells. Immunohistochemistry for mesenchymal markers, matrix <t>metalloproteinase-2</t> (C) and -9 (D) . Both matrix metalloproteinase-2 (C) and -9 (D) are expressed in the tumor cells.
    Matrix Metalloproteinase 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/matrix metalloproteinase 2/product/Thermo Fisher
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    matrix metalloproteinase 2 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    99
    Thermo Fisher collagenase type iv
    Epithelial-mesenchymal transition in the present case. Immunohistochemistry for transcriptional factors, Slug (A) and Twist (B) . Nuclear staining for Slug and Twist was observed in the neoplastic cells. Immunohistochemistry for mesenchymal markers, matrix <t>metalloproteinase-2</t> (C) and -9 (D) . Both matrix metalloproteinase-2 (C) and -9 (D) are expressed in the tumor cells.
    Collagenase Type Iv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type iv/product/Thermo Fisher
    Average 99 stars, based on 365 article reviews
    Price from $9.99 to $1999.99
    collagenase type iv - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Instability of the newly formed cartilage tissue and subchondral bone plate migration in small size defects (A): Representative SafO staining and immunohistochemical staining for type 2 collagen and type 1 collagen of small osteochondral defects at 16 weeks. (Bar = 500 μm. Yellow dot line in 0.9 mm group indicates subchondral bone plate. Black dotted line indicated the original defect borders) (B): Quantification of proportion of SafO positivity in which 100% indicated same size as native cartilage in the small defect size group until 16 weeks. (C): Quantification of the relative proportion of the subchondral bone area in which 100% indicated same size as the drilling size in the 0.9 mm group until 16 weeks. (D): Quantification for areas of subchondral bone plate distance in which 0 mm indicated same depth as native subchondral bone plate depth in the 0.9 mm group until 16 weeks. ( n = 4, * P

    Journal: Osteoarthritis and Cartilage

    Article Title: Definition of a Critical Size Osteochondral Knee Defect and its Negative Effect on the Surrounding Articular Cartilage in the Rat

    doi: 10.1016/j.joca.2017.05.006

    Figure Lengend Snippet: Instability of the newly formed cartilage tissue and subchondral bone plate migration in small size defects (A): Representative SafO staining and immunohistochemical staining for type 2 collagen and type 1 collagen of small osteochondral defects at 16 weeks. (Bar = 500 μm. Yellow dot line in 0.9 mm group indicates subchondral bone plate. Black dotted line indicated the original defect borders) (B): Quantification of proportion of SafO positivity in which 100% indicated same size as native cartilage in the small defect size group until 16 weeks. (C): Quantification of the relative proportion of the subchondral bone area in which 100% indicated same size as the drilling size in the 0.9 mm group until 16 weeks. (D): Quantification for areas of subchondral bone plate distance in which 0 mm indicated same depth as native subchondral bone plate depth in the 0.9 mm group until 16 weeks. ( n = 4, * P

    Article Snippet: Isolation and culture of rat chondrocytes Intact cartilage (non-operated positive control) from rat knee joints was dissected and digested with 0.2% type 2 collagenase (Thermo Fisher Scientific) in DMEM/F12 for 3 h at 37°C.

    Techniques: Migration, Staining, Immunohistochemistry

    Immunohistochemical characterization and gene expression of the reparative tissues (A): Representative immunohistochemically staining of native cartilage and four different sizes of osteochondral defects for type 2 collagen at 4 and 8 weeks ( n = 4). (B): Representative immunohistochemically staining of native cartilage and four different size of osteochondral defects for type 1 collagen at 4 and 8 weeks ( n = 4). (C): Quantitative gene expression analysis the newly formed tissue in 1.4 mm osteochondral defects and non-operative native cartilage ( n = 3). (Bar = 500 μm).

    Journal: Osteoarthritis and Cartilage

    Article Title: Definition of a Critical Size Osteochondral Knee Defect and its Negative Effect on the Surrounding Articular Cartilage in the Rat

    doi: 10.1016/j.joca.2017.05.006

    Figure Lengend Snippet: Immunohistochemical characterization and gene expression of the reparative tissues (A): Representative immunohistochemically staining of native cartilage and four different sizes of osteochondral defects for type 2 collagen at 4 and 8 weeks ( n = 4). (B): Representative immunohistochemically staining of native cartilage and four different size of osteochondral defects for type 1 collagen at 4 and 8 weeks ( n = 4). (C): Quantitative gene expression analysis the newly formed tissue in 1.4 mm osteochondral defects and non-operative native cartilage ( n = 3). (Bar = 500 μm).

    Article Snippet: Isolation and culture of rat chondrocytes Intact cartilage (non-operated positive control) from rat knee joints was dissected and digested with 0.2% type 2 collagenase (Thermo Fisher Scientific) in DMEM/F12 for 3 h at 37°C.

    Techniques: Immunohistochemistry, Expressing, Staining

    Epithelial-mesenchymal transition in the present case. Immunohistochemistry for transcriptional factors, Slug (A) and Twist (B) . Nuclear staining for Slug and Twist was observed in the neoplastic cells. Immunohistochemistry for mesenchymal markers, matrix metalloproteinase-2 (C) and -9 (D) . Both matrix metalloproteinase-2 (C) and -9 (D) are expressed in the tumor cells.

    Journal: BMC Research Notes

    Article Title: Intracranial extension of adenoid cystic carcinoma: potential involvement of EphA2 expression and epithelial-mesenchymal transition in tumor metastasis: a case report

    doi: 10.1186/1756-0500-7-131

    Figure Lengend Snippet: Epithelial-mesenchymal transition in the present case. Immunohistochemistry for transcriptional factors, Slug (A) and Twist (B) . Nuclear staining for Slug and Twist was observed in the neoplastic cells. Immunohistochemistry for mesenchymal markers, matrix metalloproteinase-2 (C) and -9 (D) . Both matrix metalloproteinase-2 (C) and -9 (D) are expressed in the tumor cells.

    Article Snippet: Antibodies Antibodies were purchased as follows: rabbit monoclonal to E-cadherin (3195, Cell Signalling, Boston, MA, USA; dilution, 1:400) and Slug (9585, Cell Signalling; dilution, 1:100), mouse monoclonal to Vimentin (m0725, DakoCytomation, Kyoto, Japan: dilution, 1:2500), Twist (ab50887, Abcam, Cambridge, UK; dilution, 1:250) and matrix metalloproteinase-2 (MMP-2) (MS804, ThermoFisher Scientific, Cheshire, UK; dilution, 1:100) and rabbit polyclonal to EphA2 (sc-924, Santa Cruz Biotechnology, CA USA; dilution, 1:50), ephrinA1 (sc-20719, Santa Cruz Biotechnology; dilution, 1:50), MMP-9 (RB9234, ThermoFisher Scientific; dilution, 1:500).

    Techniques: Immunohistochemistry, Staining