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Roche collagenase type 2
Collagenase Type 2, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/collagenase type 2/product/Roche
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
collagenase type 2 - by Bioz Stars, 2020-05
92/100 stars

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Lysis:

Article Title: Interleukin-17A is involved in development of spontaneous pulmonary emphysema caused by Toll-like receptor 4 mutation
Article Snippet: .. The lung was minced; digested with 1 mL digestion medium consisting of RPMI-1640, 1 mg/mL collagenase type 2 (Roche Diagnostics; Indianapolis, IN, USA) and 0.02 mg/mL DNase I (grade II from bovine pancreas) for 60 min at 37 °C; subjected to red blood cell lysis (eBioscience); passed through a 100 μm cell strainer; and kept on ice until labeling. .. Flow cytometry The Th1/Th2 mouse T cell subpopulations in single-cell lung suspensions were labeled with FITC-conjugated anti-CD4, PE-conjugated anti-IFN-γ, and Alexa 647-conjugated anti-IL-13 antibodies.

Labeling:

Article Title: Interleukin-17A is involved in development of spontaneous pulmonary emphysema caused by Toll-like receptor 4 mutation
Article Snippet: .. The lung was minced; digested with 1 mL digestion medium consisting of RPMI-1640, 1 mg/mL collagenase type 2 (Roche Diagnostics; Indianapolis, IN, USA) and 0.02 mg/mL DNase I (grade II from bovine pancreas) for 60 min at 37 °C; subjected to red blood cell lysis (eBioscience); passed through a 100 μm cell strainer; and kept on ice until labeling. .. Flow cytometry The Th1/Th2 mouse T cell subpopulations in single-cell lung suspensions were labeled with FITC-conjugated anti-CD4, PE-conjugated anti-IFN-γ, and Alexa 647-conjugated anti-IL-13 antibodies.

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  • 90
    Roche materials gelatinase a
    Panel a Representative gelatin zymography of serum ( lanes 1–6 ), purified gelatinases, and Western blotting. Molecular weight are shown on the left. Lane 1 healthy subject, lane 2 glioblastoma multiforme (patient 11); lane 3 oligodendroglioma (patient 5), lane 4 intracranial metastasis from melanoma (patient 57), lane 5 low-grade meningioma (patient 25), lane 6 high-grade meningioma (patient 43), lane 7 purified gelatinase B (MMP-9, 92 kDa) 20 µU, lane 8 purified <t>gelatinase</t> A (MMP-2, 72 kDa) 120 mU, lane 9 Western blotting of MMP-9 (92 kDa); lane 10 : Western blotting of MMP-2 (72 kDa). Panel b Box plot showing the distribution of multimeric form (240 kDa) and monomeric form (92 kDa) of serum MMP-9 in healthy controls, low-grade meningioma (grade I), high-grade meningioma (grade II), high-grade glioma (GB) and intracranial metastases. Value of integrated density are expressed as volume ×10 −3 . Data are shown as median ( horizontal line in the box), Q1 and Q3 ( border of the box) and min and max ( whiskers outside the box). Dot represent outliers values (i.e., data points below Q1 −1.5 × IQR or above Q3 +1.5 × IQR). Q1 = 25th percentile; Q3 = 75th percentile; IQR (interquartile range) = Q3–Q1. Panel c Immunohistochemestry against MMP-9 in gliomas; (a) grade II glioma (oligodendroglioma): diffuse cytoplasmic and nuclear immunoreactivity in neoplastic cells (400× magnification); (b) grade III glioma (anaplastic oligodendroglioma): strong cytoplasmic signal inside tumor cells (400× magnification); (c) grade IV glioma (glioblastoma): heavy immunoreactivity in neoplastic endothelial cells lying at the interface of tumor-cerebral parenchyma (200× magnification); (d) grade IV glioma (glioblastoma): deep immunoreactivity in neoplastic vessel endothelial cells and occasional cytoplasmic signal within the tumor (400× magnification). Panel d Immunohistochemestry against MMP-9 in meningiomas (400× magnification) and box plot showing MMP-9 staining percentage in low- and high-grade meningiomas. (a) grade I meningioma: cytoplasmic immunoreaction in less than 10 % of neoplastic cells (b) grade II meningioma: cytoplasmic immunoreaction in 10–30 % of neoplastic cells (c) grade II meningioma: cytoplasmic immunoreaction in more than 30 % of neoplastic cells
    Materials Gelatinase A, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials gelatinase a/product/Roche
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    materials gelatinase a - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    89
    Roche collagenase type d
    Panel a Representative gelatin zymography of serum ( lanes 1–6 ), purified gelatinases, and Western blotting. Molecular weight are shown on the left. Lane 1 healthy subject, lane 2 glioblastoma multiforme (patient 11); lane 3 oligodendroglioma (patient 5), lane 4 intracranial metastasis from melanoma (patient 57), lane 5 low-grade meningioma (patient 25), lane 6 high-grade meningioma (patient 43), lane 7 purified gelatinase B (MMP-9, 92 kDa) 20 µU, lane 8 purified <t>gelatinase</t> A (MMP-2, 72 kDa) 120 mU, lane 9 Western blotting of MMP-9 (92 kDa); lane 10 : Western blotting of MMP-2 (72 kDa). Panel b Box plot showing the distribution of multimeric form (240 kDa) and monomeric form (92 kDa) of serum MMP-9 in healthy controls, low-grade meningioma (grade I), high-grade meningioma (grade II), high-grade glioma (GB) and intracranial metastases. Value of integrated density are expressed as volume ×10 −3 . Data are shown as median ( horizontal line in the box), Q1 and Q3 ( border of the box) and min and max ( whiskers outside the box). Dot represent outliers values (i.e., data points below Q1 −1.5 × IQR or above Q3 +1.5 × IQR). Q1 = 25th percentile; Q3 = 75th percentile; IQR (interquartile range) = Q3–Q1. Panel c Immunohistochemestry against MMP-9 in gliomas; (a) grade II glioma (oligodendroglioma): diffuse cytoplasmic and nuclear immunoreactivity in neoplastic cells (400× magnification); (b) grade III glioma (anaplastic oligodendroglioma): strong cytoplasmic signal inside tumor cells (400× magnification); (c) grade IV glioma (glioblastoma): heavy immunoreactivity in neoplastic endothelial cells lying at the interface of tumor-cerebral parenchyma (200× magnification); (d) grade IV glioma (glioblastoma): deep immunoreactivity in neoplastic vessel endothelial cells and occasional cytoplasmic signal within the tumor (400× magnification). Panel d Immunohistochemestry against MMP-9 in meningiomas (400× magnification) and box plot showing MMP-9 staining percentage in low- and high-grade meningiomas. (a) grade I meningioma: cytoplasmic immunoreaction in less than 10 % of neoplastic cells (b) grade II meningioma: cytoplasmic immunoreaction in 10–30 % of neoplastic cells (c) grade II meningioma: cytoplasmic immunoreaction in more than 30 % of neoplastic cells
    Collagenase Type D, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type d/product/Roche
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    collagenase type d - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    Image Search Results


    Panel a Representative gelatin zymography of serum ( lanes 1–6 ), purified gelatinases, and Western blotting. Molecular weight are shown on the left. Lane 1 healthy subject, lane 2 glioblastoma multiforme (patient 11); lane 3 oligodendroglioma (patient 5), lane 4 intracranial metastasis from melanoma (patient 57), lane 5 low-grade meningioma (patient 25), lane 6 high-grade meningioma (patient 43), lane 7 purified gelatinase B (MMP-9, 92 kDa) 20 µU, lane 8 purified gelatinase A (MMP-2, 72 kDa) 120 mU, lane 9 Western blotting of MMP-9 (92 kDa); lane 10 : Western blotting of MMP-2 (72 kDa). Panel b Box plot showing the distribution of multimeric form (240 kDa) and monomeric form (92 kDa) of serum MMP-9 in healthy controls, low-grade meningioma (grade I), high-grade meningioma (grade II), high-grade glioma (GB) and intracranial metastases. Value of integrated density are expressed as volume ×10 −3 . Data are shown as median ( horizontal line in the box), Q1 and Q3 ( border of the box) and min and max ( whiskers outside the box). Dot represent outliers values (i.e., data points below Q1 −1.5 × IQR or above Q3 +1.5 × IQR). Q1 = 25th percentile; Q3 = 75th percentile; IQR (interquartile range) = Q3–Q1. Panel c Immunohistochemestry against MMP-9 in gliomas; (a) grade II glioma (oligodendroglioma): diffuse cytoplasmic and nuclear immunoreactivity in neoplastic cells (400× magnification); (b) grade III glioma (anaplastic oligodendroglioma): strong cytoplasmic signal inside tumor cells (400× magnification); (c) grade IV glioma (glioblastoma): heavy immunoreactivity in neoplastic endothelial cells lying at the interface of tumor-cerebral parenchyma (200× magnification); (d) grade IV glioma (glioblastoma): deep immunoreactivity in neoplastic vessel endothelial cells and occasional cytoplasmic signal within the tumor (400× magnification). Panel d Immunohistochemestry against MMP-9 in meningiomas (400× magnification) and box plot showing MMP-9 staining percentage in low- and high-grade meningiomas. (a) grade I meningioma: cytoplasmic immunoreaction in less than 10 % of neoplastic cells (b) grade II meningioma: cytoplasmic immunoreaction in 10–30 % of neoplastic cells (c) grade II meningioma: cytoplasmic immunoreaction in more than 30 % of neoplastic cells

    Journal: Journal of Neuro-Oncology

    Article Title: Evaluation of matrix metalloproteinase type IV-collagenases in serum of patients with tumors of the central nervous system

    doi: 10.1007/s11060-016-2297-4

    Figure Lengend Snippet: Panel a Representative gelatin zymography of serum ( lanes 1–6 ), purified gelatinases, and Western blotting. Molecular weight are shown on the left. Lane 1 healthy subject, lane 2 glioblastoma multiforme (patient 11); lane 3 oligodendroglioma (patient 5), lane 4 intracranial metastasis from melanoma (patient 57), lane 5 low-grade meningioma (patient 25), lane 6 high-grade meningioma (patient 43), lane 7 purified gelatinase B (MMP-9, 92 kDa) 20 µU, lane 8 purified gelatinase A (MMP-2, 72 kDa) 120 mU, lane 9 Western blotting of MMP-9 (92 kDa); lane 10 : Western blotting of MMP-2 (72 kDa). Panel b Box plot showing the distribution of multimeric form (240 kDa) and monomeric form (92 kDa) of serum MMP-9 in healthy controls, low-grade meningioma (grade I), high-grade meningioma (grade II), high-grade glioma (GB) and intracranial metastases. Value of integrated density are expressed as volume ×10 −3 . Data are shown as median ( horizontal line in the box), Q1 and Q3 ( border of the box) and min and max ( whiskers outside the box). Dot represent outliers values (i.e., data points below Q1 −1.5 × IQR or above Q3 +1.5 × IQR). Q1 = 25th percentile; Q3 = 75th percentile; IQR (interquartile range) = Q3–Q1. Panel c Immunohistochemestry against MMP-9 in gliomas; (a) grade II glioma (oligodendroglioma): diffuse cytoplasmic and nuclear immunoreactivity in neoplastic cells (400× magnification); (b) grade III glioma (anaplastic oligodendroglioma): strong cytoplasmic signal inside tumor cells (400× magnification); (c) grade IV glioma (glioblastoma): heavy immunoreactivity in neoplastic endothelial cells lying at the interface of tumor-cerebral parenchyma (200× magnification); (d) grade IV glioma (glioblastoma): deep immunoreactivity in neoplastic vessel endothelial cells and occasional cytoplasmic signal within the tumor (400× magnification). Panel d Immunohistochemestry against MMP-9 in meningiomas (400× magnification) and box plot showing MMP-9 staining percentage in low- and high-grade meningiomas. (a) grade I meningioma: cytoplasmic immunoreaction in less than 10 % of neoplastic cells (b) grade II meningioma: cytoplasmic immunoreaction in 10–30 % of neoplastic cells (c) grade II meningioma: cytoplasmic immunoreaction in more than 30 % of neoplastic cells

    Article Snippet: Materials Gelatinase A and gelatinase B were purchased from Hoffmann-La Roche Ltd (Basel, Switzerland).

    Techniques: Zymography, Purification, Western Blot, Molecular Weight, Staining

    Western blot analysis of Notch-1, MMP-2 and VEGF proteins in MG-63 cells with cannabinoid and/or ADM treatments. β-actin was used as loading control. MMP-2, matrix metalloproteinase-2; VEGF, vascular endothelial growth factor; ADM, adriamycin.

    Journal: Oncology Letters

    Article Title: Potentiation of the antitumor activity of adriamycin against osteosarcoma by cannabinoid WIN-55,212-2

    doi: 10.3892/ol.2015.3525

    Figure Lengend Snippet: Western blot analysis of Notch-1, MMP-2 and VEGF proteins in MG-63 cells with cannabinoid and/or ADM treatments. β-actin was used as loading control. MMP-2, matrix metalloproteinase-2; VEGF, vascular endothelial growth factor; ADM, adriamycin.

    Article Snippet: The transcript levels of Notch-1, matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) were detected by RT-qPCR using a Light Cycler 2.0 (Roche Diagnostics Deutschland GmbH, Mannheim, Germany) using SYBR® Green Master Mix (Toyobo, Osaka, Japan).

    Techniques: Western Blot

    The effects on protease and anti-protease in elastase induced emphysema with or without mesenchymal stem cells (MSCs). C57BL/6J mice were intratracheally applied with 0.4 U of elastase on day 0 and then intravenously injected with MSCs on day 7. Lung tissues were collected on day 14 (n=6-9 per group). (A) Matrix metalloproteinase (MMP) 2, MMP9, and MMP12 mRNA expression were measured with quantitative polymerase chain reaction (PCR) using SYBR Green and normalized by β-actin expression and then displayed with ratio to control group. Values are presented as the mean±SEM. * p

    Journal: Tuberculosis and Respiratory Diseases

    Article Title: The Therapeutic Effects of Optimal Dose of Mesenchymal Stem Cells in a Murine Model of an Elastase Induced-Emphysema

    doi: 10.4046/trd.2015.78.3.239

    Figure Lengend Snippet: The effects on protease and anti-protease in elastase induced emphysema with or without mesenchymal stem cells (MSCs). C57BL/6J mice were intratracheally applied with 0.4 U of elastase on day 0 and then intravenously injected with MSCs on day 7. Lung tissues were collected on day 14 (n=6-9 per group). (A) Matrix metalloproteinase (MMP) 2, MMP9, and MMP12 mRNA expression were measured with quantitative polymerase chain reaction (PCR) using SYBR Green and normalized by β-actin expression and then displayed with ratio to control group. Values are presented as the mean±SEM. * p

    Article Snippet: Quantitative polymerase chain reaction (PCR) analyses for matrix metalloproteinase (MMP) 2, MMP9, MMP12, SLIP, and tissue inhibitor of metalloproteinases-1 (TIMP1) were performed using a real-time LightCycler 480 PCR system (Roche Diagnostics, Basel, Switzerland) with LightCycler 480 SYBR Green I master (Roche Diagnostics).

    Techniques: Mouse Assay, Injection, Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, SYBR Green Assay