Journal: Journal of Neuro-Oncology
Article Title: Evaluation of matrix metalloproteinase type IV-collagenases in serum of patients with tumors of the central nervous system
Figure Lengend Snippet: Panel a Representative gelatin zymography of serum ( lanes 1–6 ), purified gelatinases, and Western blotting. Molecular weight are shown on the left. Lane 1 healthy subject, lane 2 glioblastoma multiforme (patient 11); lane 3 oligodendroglioma (patient 5), lane 4 intracranial metastasis from melanoma (patient 57), lane 5 low-grade meningioma (patient 25), lane 6 high-grade meningioma (patient 43), lane 7 purified gelatinase B (MMP-9, 92 kDa) 20 µU, lane 8 purified gelatinase A (MMP-2, 72 kDa) 120 mU, lane 9 Western blotting of MMP-9 (92 kDa); lane 10 : Western blotting of MMP-2 (72 kDa). Panel b Box plot showing the distribution of multimeric form (240 kDa) and monomeric form (92 kDa) of serum MMP-9 in healthy controls, low-grade meningioma (grade I), high-grade meningioma (grade II), high-grade glioma (GB) and intracranial metastases. Value of integrated density are expressed as volume ×10 −3 . Data are shown as median ( horizontal line in the box), Q1 and Q3 ( border of the box) and min and max ( whiskers outside the box). Dot represent outliers values (i.e., data points below Q1 −1.5 × IQR or above Q3 +1.5 × IQR). Q1 = 25th percentile; Q3 = 75th percentile; IQR (interquartile range) = Q3–Q1. Panel c Immunohistochemestry against MMP-9 in gliomas; (a) grade II glioma (oligodendroglioma): diffuse cytoplasmic and nuclear immunoreactivity in neoplastic cells (400× magnification); (b) grade III glioma (anaplastic oligodendroglioma): strong cytoplasmic signal inside tumor cells (400× magnification); (c) grade IV glioma (glioblastoma): heavy immunoreactivity in neoplastic endothelial cells lying at the interface of tumor-cerebral parenchyma (200× magnification); (d) grade IV glioma (glioblastoma): deep immunoreactivity in neoplastic vessel endothelial cells and occasional cytoplasmic signal within the tumor (400× magnification). Panel d Immunohistochemestry against MMP-9 in meningiomas (400× magnification) and box plot showing MMP-9 staining percentage in low- and high-grade meningiomas. (a) grade I meningioma: cytoplasmic immunoreaction in less than 10 % of neoplastic cells (b) grade II meningioma: cytoplasmic immunoreaction in 10–30 % of neoplastic cells (c) grade II meningioma: cytoplasmic immunoreaction in more than 30 % of neoplastic cells
Article Snippet: Materials Gelatinase A and gelatinase B were purchased from Hoffmann-La Roche Ltd (Basel, Switzerland).
Techniques: Zymography, Purification, Western Blot, Molecular Weight, Staining