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Boehringer Mannheim collagenase type 2
Collagenase Type 2, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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collagenase type 2 - by Bioz Stars, 2020-05
92/100 stars

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Article Title: Quantitative Analysis of Myocardial Inflammation by Flow Cytometry in Murine Autoimmune Myocarditis
Article Snippet: Enzymatic digestion was initiated by perfusion with the above solution with the addition of collagenase type 2 and protease type XIV (0.895 mg/ml and 0.5 mg/ml respectively; Boehringer Mannheim, Indianapolis, IN) for an additional 7 minutes.

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  • 90
    Boehringer Mannheim mmp 2
    Effect of CHX on <t>MMP-2</t> and -9 activity. MMPs activated with APMA, CHX, and gelatin were incubated for 18 h at 37°C. Gelatin degradation was monitored by SDS–7.5% PAGE and Coomassie blue staining. (A) Lanes: 1, gelatin alone; 2, MMP-2 and gelatin; 3, MMP-2, gelatin, and 10 mM EDTA; 4, MMP-2, gelatin, and 0.03% CHX; 5, MMP-2, gelatin, and 0.004% CHX; 6, MMP-2, gelatin, 0.03% CHX, and 0.75 mM calcium chloride; 7, MMP-2, gelatin, 0.004% CHX, and 0.75 mM calcium chloride. (B) Lanes: 1, gelatin alone; 2, MMP-9 and gelatin; 3, MMP-9, gelatin, and 10 mM EDTA; 4, MMP-9, gelatin, and 0.03% CHX; 5, MMP-9, gelatin, 0.03% CHX, and 0.75 mM calcium chloride. Molecular mass markers were, from top to bottom, myosin (200 kDa), phosphorylase b (97.4 kDa), and bovine serum albumin (68 kDa).
    Mmp 2, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Boehringer Mannheim gelatinase a
    (A) An scFv derived from the antibody LMH2, which recognizes HMW-MAA, was fused to the N terminus of MLV-A 4070A SU by using the proline-rich region from MLV-A 4070A env (nucleotides 751 to 927) (P) as a spacer to make scLPA. An MMP cleavage site (PLGLWA) was introduced between the P linker and the SU of the MLV-A envelope to make scLPMA. TM, transmembrane protein. (B) Detection of unmodified MLV-A 4070A envelope (lane 1A), scLPA, and scLPMA in viral pellets from producer cell lines. p30 gag was detected in the same pellets to quantitate viral particles. (C) Cleavage of scLPMA by <t>gelatinase</t> A (Gel A) as described in Materials and Methods. Equal amounts of unmodified MLV-A 4070A and scLPMA envelopes were loaded in each lane. kd, kilodaltons.
    Gelatinase A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of CHX on MMP-2 and -9 activity. MMPs activated with APMA, CHX, and gelatin were incubated for 18 h at 37°C. Gelatin degradation was monitored by SDS–7.5% PAGE and Coomassie blue staining. (A) Lanes: 1, gelatin alone; 2, MMP-2 and gelatin; 3, MMP-2, gelatin, and 10 mM EDTA; 4, MMP-2, gelatin, and 0.03% CHX; 5, MMP-2, gelatin, and 0.004% CHX; 6, MMP-2, gelatin, 0.03% CHX, and 0.75 mM calcium chloride; 7, MMP-2, gelatin, 0.004% CHX, and 0.75 mM calcium chloride. (B) Lanes: 1, gelatin alone; 2, MMP-9 and gelatin; 3, MMP-9, gelatin, and 10 mM EDTA; 4, MMP-9, gelatin, and 0.03% CHX; 5, MMP-9, gelatin, 0.03% CHX, and 0.75 mM calcium chloride. Molecular mass markers were, from top to bottom, myosin (200 kDa), phosphorylase b (97.4 kDa), and bovine serum albumin (68 kDa).

    Journal: Clinical and Diagnostic Laboratory Immunology

    Article Title: Inhibition of the Activities of Matrix Metalloproteinases 2, 8, and 9 by Chlorhexidine

    doi:

    Figure Lengend Snippet: Effect of CHX on MMP-2 and -9 activity. MMPs activated with APMA, CHX, and gelatin were incubated for 18 h at 37°C. Gelatin degradation was monitored by SDS–7.5% PAGE and Coomassie blue staining. (A) Lanes: 1, gelatin alone; 2, MMP-2 and gelatin; 3, MMP-2, gelatin, and 10 mM EDTA; 4, MMP-2, gelatin, and 0.03% CHX; 5, MMP-2, gelatin, and 0.004% CHX; 6, MMP-2, gelatin, 0.03% CHX, and 0.75 mM calcium chloride; 7, MMP-2, gelatin, 0.004% CHX, and 0.75 mM calcium chloride. (B) Lanes: 1, gelatin alone; 2, MMP-9 and gelatin; 3, MMP-9, gelatin, and 10 mM EDTA; 4, MMP-9, gelatin, and 0.03% CHX; 5, MMP-9, gelatin, 0.03% CHX, and 0.75 mM calcium chloride. Molecular mass markers were, from top to bottom, myosin (200 kDa), phosphorylase b (97.4 kDa), and bovine serum albumin (68 kDa).

    Article Snippet: Two genetically distinct pure human gelatinases were used: MMP-2 (72-kDa gelatinase A isolated from human fibrosarcoma cells and purchased from Boehringer Mannheim, Montréal, Québec, Canada) and MMP-9 (human recombinant 92-kDa gelatinase B isolated from mammalian cells and purchased from Oncogene Research Products, Cambridge, Mass.).

    Techniques: Activity Assay, Incubation, Polyacrylamide Gel Electrophoresis, Staining

    (A) An scFv derived from the antibody LMH2, which recognizes HMW-MAA, was fused to the N terminus of MLV-A 4070A SU by using the proline-rich region from MLV-A 4070A env (nucleotides 751 to 927) (P) as a spacer to make scLPA. An MMP cleavage site (PLGLWA) was introduced between the P linker and the SU of the MLV-A envelope to make scLPMA. TM, transmembrane protein. (B) Detection of unmodified MLV-A 4070A envelope (lane 1A), scLPA, and scLPMA in viral pellets from producer cell lines. p30 gag was detected in the same pellets to quantitate viral particles. (C) Cleavage of scLPMA by gelatinase A (Gel A) as described in Materials and Methods. Equal amounts of unmodified MLV-A 4070A and scLPMA envelopes were loaded in each lane. kd, kilodaltons.

    Journal: Journal of Virology

    Article Title: Retrovirus Targeting by Tropism Restriction to Melanoma Cells

    doi:

    Figure Lengend Snippet: (A) An scFv derived from the antibody LMH2, which recognizes HMW-MAA, was fused to the N terminus of MLV-A 4070A SU by using the proline-rich region from MLV-A 4070A env (nucleotides 751 to 927) (P) as a spacer to make scLPA. An MMP cleavage site (PLGLWA) was introduced between the P linker and the SU of the MLV-A envelope to make scLPMA. TM, transmembrane protein. (B) Detection of unmodified MLV-A 4070A envelope (lane 1A), scLPA, and scLPMA in viral pellets from producer cell lines. p30 gag was detected in the same pellets to quantitate viral particles. (C) Cleavage of scLPMA by gelatinase A (Gel A) as described in Materials and Methods. Equal amounts of unmodified MLV-A 4070A and scLPMA envelopes were loaded in each lane. kd, kilodaltons.

    Article Snippet: Some cleavage of scLPMA was observed without gelatinase A, probably due to production of MMPs by the TELCeB6 cells (Fig. C).

    Techniques: Derivative Assay