Journal: Clinical and Diagnostic Laboratory Immunology
Article Title: Inhibition of the Activities of Matrix Metalloproteinases 2, 8, and 9 by Chlorhexidine
Figure Lengend Snippet: Effect of CHX on MMP-2 and -9 activity. MMPs activated with APMA, CHX, and gelatin were incubated for 18 h at 37°C. Gelatin degradation was monitored by SDS–7.5% PAGE and Coomassie blue staining. (A) Lanes: 1, gelatin alone; 2, MMP-2 and gelatin; 3, MMP-2, gelatin, and 10 mM EDTA; 4, MMP-2, gelatin, and 0.03% CHX; 5, MMP-2, gelatin, and 0.004% CHX; 6, MMP-2, gelatin, 0.03% CHX, and 0.75 mM calcium chloride; 7, MMP-2, gelatin, 0.004% CHX, and 0.75 mM calcium chloride. (B) Lanes: 1, gelatin alone; 2, MMP-9 and gelatin; 3, MMP-9, gelatin, and 10 mM EDTA; 4, MMP-9, gelatin, and 0.03% CHX; 5, MMP-9, gelatin, 0.03% CHX, and 0.75 mM calcium chloride. Molecular mass markers were, from top to bottom, myosin (200 kDa), phosphorylase b (97.4 kDa), and bovine serum albumin (68 kDa).
Article Snippet: Two genetically distinct pure human gelatinases were used: MMP-2 (72-kDa gelatinase A isolated from human fibrosarcoma cells and purchased from Boehringer Mannheim, Montréal, Québec, Canada) and MMP-9 (human recombinant 92-kDa gelatinase B isolated from mammalian cells and purchased from Oncogene Research Products, Cambridge, Mass.).
Techniques: Activity Assay, Incubation, Polyacrylamide Gel Electrophoresis, Staining