collagenase iv  (Millipore)


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    Structured Review

    Millipore collagenase iv
    Collagenase Iv, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase iv/product/Millipore
    Average 97 stars, based on 218 article reviews
    Price from $9.99 to $1999.99
    collagenase iv - by Bioz Stars, 2020-05
    97/100 stars

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    Isolation:

    Article Title: MiR-663a Stimulates Proliferation and Suppresses Early Apoptosis of Human Spermatogonial Stem Cells by Targeting NFIX and Regulating Cell Cycle
    Article Snippet: .. Seminiferous tubules were isolated from human testis tissues by the first enzymatic digestion comprising 2 mg/mL collagenase IV (Gibco) and 1 mg/mL DNase I (Gibco) in 34°C water bath for 15 min. After sedimentation, human male germ cells, Sertoli cells, and myoid cells were obtained from seminiferous tubules using a second enzymatic digestion with 4 mg/mL collagenase IV, 2.5 mg/mL hyaluronidase (Sigma), 2 mg/mL trypsin (Sigma), and 1 mg/mL DNase I. .. Differential plating of the mixed cells was performed in a 10-cm-diameter cell culture dish in DMEM/F12 supplemented with 10% fetal bovine serum (FBS) (Gibco) at 34°C in 5% CO2 for 1 day.

    Purification:

    Article Title: Regulation of Epithelial Injury and Bile Duct Obstruction by NLRP3 and IL-1R1 in Experimental Biliary Atresia
    Article Snippet: .. Hepatic DCs were purified from minced liver tissue incubated with collagenase IV (SigmaAldrich) for 30 minutes at 37°C in RPMI and centrifuged in 33% percoll. .. Single cell suspension of DCs was obtained by MACS according to the manufacturer’s protocol (Miltenyi Biotec).

    Sedimentation:

    Article Title: MiR-663a Stimulates Proliferation and Suppresses Early Apoptosis of Human Spermatogonial Stem Cells by Targeting NFIX and Regulating Cell Cycle
    Article Snippet: .. Seminiferous tubules were isolated from human testis tissues by the first enzymatic digestion comprising 2 mg/mL collagenase IV (Gibco) and 1 mg/mL DNase I (Gibco) in 34°C water bath for 15 min. After sedimentation, human male germ cells, Sertoli cells, and myoid cells were obtained from seminiferous tubules using a second enzymatic digestion with 4 mg/mL collagenase IV, 2.5 mg/mL hyaluronidase (Sigma), 2 mg/mL trypsin (Sigma), and 1 mg/mL DNase I. .. Differential plating of the mixed cells was performed in a 10-cm-diameter cell culture dish in DMEM/F12 supplemented with 10% fetal bovine serum (FBS) (Gibco) at 34°C in 5% CO2 for 1 day.

    Incubation:

    Article Title: Roles of Type 1, 2, and 3 Innate Lymphoid Cells in Herpes Simplex Virus 1 Infection In Vitro and In Vivo
    Article Snippet: .. The pieces were then further cut and incubated at 37°C for 20 min in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS, 10 mM HEPES, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 500 U/ml collagenase IV, and 50 U/ml DNase I (Sigma-Aldrich). .. The solution containing digested tissue was passed through a 70-μm cell strainer, and the lymphocytes were enriched by centrifugation in 40% Percoll.

    Article Title: Regulation of Epithelial Injury and Bile Duct Obstruction by NLRP3 and IL-1R1 in Experimental Biliary Atresia
    Article Snippet: .. Hepatic DCs were purified from minced liver tissue incubated with collagenase IV (SigmaAldrich) for 30 minutes at 37°C in RPMI and centrifuged in 33% percoll. .. Single cell suspension of DCs was obtained by MACS according to the manufacturer’s protocol (Miltenyi Biotec).

    Article Title: CD8α+ dendritic cells dictate leukemia-specific CD8+ T cell fates
    Article Snippet: .. Lymphoid organs were injected with 5ml of 1mg/ml collagenase IV (Sigma) and 20μg/ml DNAse I (Roche), incubated at 37° C for 30 minutes, and passed through a 70μm filter. ..

    Modification:

    Article Title: Roles of Type 1, 2, and 3 Innate Lymphoid Cells in Herpes Simplex Virus 1 Infection In Vitro and In Vivo
    Article Snippet: .. The pieces were then further cut and incubated at 37°C for 20 min in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS, 10 mM HEPES, 100 U/ml penicillin, 0.1 mg/ml streptomycin, 500 U/ml collagenase IV, and 50 U/ml DNase I (Sigma-Aldrich). .. The solution containing digested tissue was passed through a 70-μm cell strainer, and the lymphocytes were enriched by centrifugation in 40% Percoll.

    Injection:

    Article Title: CD8α+ dendritic cells dictate leukemia-specific CD8+ T cell fates
    Article Snippet: .. Lymphoid organs were injected with 5ml of 1mg/ml collagenase IV (Sigma) and 20μg/ml DNAse I (Roche), incubated at 37° C for 30 minutes, and passed through a 70μm filter. ..

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  • 99
    Millipore collagenase iv
    Collagenase Iv, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase iv/product/Millipore
    Average 99 stars, based on 715 article reviews
    Price from $9.99 to $1999.99
    collagenase iv - by Bioz Stars, 2020-05
    99/100 stars
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