collagenase iv  (Millipore)


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  • 99

    Structured Review

    Millipore collagenase iv
    Collagenase Iv, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 820 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase iv/product/Millipore
    Average 99 stars, based on 820 article reviews
    Price from $9.99 to $1999.99
    collagenase iv - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
    Article Snippet: .. For isolation of hepatocytes, livers were perfused with 100 CDU/ml collagenase (C5138; Sigma), and hepatocytes were purified by centrifugation at 50 g thrice. ..

    In Situ:

    Article Title: Young plasma reverses age‐dependent alterations in hepatic function through the restoration of autophagy, et al. Young plasma reverses age‐dependent alterations in hepatic function through the restoration of autophagy
    Article Snippet: .. Briefly, after anesthetizing with pentobarbital, liver was perfused in situ with warm Hank's Balanced Salt Solution (Life Technologies, 14175) via the portal vein and was followed by liver warm basic Dulbecco's Modified Eagle's Medium (DMEM, Life Technologies, 11995) containing 0.75 mg/ml collagenase D (Sigma‐Aldrich, C5138) for 10–15 min. ..

    Isolation:

    Article Title: JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss
    Article Snippet: .. Microglia and astrocytes sorting from adult mouse ventral midbrain The ventral midbrain isolated from the brain of mice anesthetized was filtered through a 70-μm nylon cell strainer and digested with collagenase D (0.05%, Sigma, C5138) and DNase I (0.025 U/ml, Sigma) for 1 h at room temperature as reported previously [ ]. .. After three washes with Hank’s Balanced Salt Solution (HBSS, Invitrogen) to remove the enzymes, cells were resuspended and then stained with allophycocyanin (APC)-labeled cluster of differentiation 45 (CD45)/Alexa Fluor 488-labeled crossing cluster of differentiation molecule 11B (CD11b), APC-labeled glutamate aspartate transporter (GLAST) antibodies, or Alexa Fluor 488/APC-labeled isotype at 4 °C for 1 h. CD11b+ / CD45low or GLAST+ populations were then sorted by the flow cytometry (Cell Lab Quanta SC; Beckman Coulter, CA).

    Article Title: Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
    Article Snippet: .. For isolation of hepatocytes, livers were perfused with 100 CDU/ml collagenase (C5138; Sigma), and hepatocytes were purified by centrifugation at 50 g thrice. ..

    Mouse Assay:

    Article Title: JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss
    Article Snippet: .. Microglia and astrocytes sorting from adult mouse ventral midbrain The ventral midbrain isolated from the brain of mice anesthetized was filtered through a 70-μm nylon cell strainer and digested with collagenase D (0.05%, Sigma, C5138) and DNase I (0.025 U/ml, Sigma) for 1 h at room temperature as reported previously [ ]. .. After three washes with Hank’s Balanced Salt Solution (HBSS, Invitrogen) to remove the enzymes, cells were resuspended and then stained with allophycocyanin (APC)-labeled cluster of differentiation 45 (CD45)/Alexa Fluor 488-labeled crossing cluster of differentiation molecule 11B (CD11b), APC-labeled glutamate aspartate transporter (GLAST) antibodies, or Alexa Fluor 488/APC-labeled isotype at 4 °C for 1 h. CD11b+ / CD45low or GLAST+ populations were then sorted by the flow cytometry (Cell Lab Quanta SC; Beckman Coulter, CA).

    Concentration Assay:

    Article Title: Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury
    Article Snippet: .. Under sterile conditions and using a stereomicroscope the DRGs were cut into smaller pieces and incubated at 37 °C in 3 mL collagenase dissolved in a dissociation solution consisting of HBSS supplemented with collagenase (concentration of 500 UI/mL; collagenase from Clostridium histolyticum C5138, Sigma Aldrich, Saint Louis, MO, USA), hyaluronidase (concentration of 150 UI/mL; hyaluronidase Type IV, Sigma Aldrich-Chemie GmbH, Buchs, Switzerland). ..

    Incubation:

    Article Title: Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues
    Article Snippet: .. Lung and liver were first cut into small pieces in DPBS, supplemented with calcium and magnesium (Sigma) and then incubated for 1 h in digestion buffer with collagenase IV (1 mg/ml, C5138, Sigma) and DNase I (0.2 mg/ml, Applichem) at 37°C with gentle agitation. .. After digestion, cells were filtered through a cell strainer (40 μm, Thermo Fisher Scientific) and resuspended in 5 mL RBC lysis buffer for 5 min. After centrifugation, the supernatant was removed.

    Article Title: Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury
    Article Snippet: .. Under sterile conditions and using a stereomicroscope the DRGs were cut into smaller pieces and incubated at 37 °C in 3 mL collagenase dissolved in a dissociation solution consisting of HBSS supplemented with collagenase (concentration of 500 UI/mL; collagenase from Clostridium histolyticum C5138, Sigma Aldrich, Saint Louis, MO, USA), hyaluronidase (concentration of 150 UI/mL; hyaluronidase Type IV, Sigma Aldrich-Chemie GmbH, Buchs, Switzerland). ..

    Article Title: The adult human testis transcriptional cell atlas
    Article Snippet: .. Briefly, testicular tissues were digested with collagenase type IV (Sigma Aldrich cat # C5138–500MG) for 5 min at 37 °C with gentle agitation (250 rpm), then shaken vigorously and incubated for another 3 min. .. The tubules were sedimented by centrifugation at 200× g for 5 min and washed with HBSS before digestion with 4.5 mL 0.25% trypsin/ethylenediaminetetraacetic acid (EDTA; Invitrogen cat # 25300054) and 4 kU DNase I (Sigma Aldrich cat # D4527–500ku).

    Modification:

    Article Title: Young plasma reverses age‐dependent alterations in hepatic function through the restoration of autophagy, et al. Young plasma reverses age‐dependent alterations in hepatic function through the restoration of autophagy
    Article Snippet: .. Briefly, after anesthetizing with pentobarbital, liver was perfused in situ with warm Hank's Balanced Salt Solution (Life Technologies, 14175) via the portal vein and was followed by liver warm basic Dulbecco's Modified Eagle's Medium (DMEM, Life Technologies, 11995) containing 0.75 mg/ml collagenase D (Sigma‐Aldrich, C5138) for 10–15 min. ..

    Purification:

    Article Title: Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
    Article Snippet: .. For isolation of hepatocytes, livers were perfused with 100 CDU/ml collagenase (C5138; Sigma), and hepatocytes were purified by centrifugation at 50 g thrice. ..

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    Millipore mmp 9
    Representative immunohistochemistry slides of paraffin-embedded GCT of bone tissue specimens for MMP-2 and <t>MMP-9</t>
    Mmp 9, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp 9/product/Millipore
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    mmp 9 - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Millipore type iv collagenase
    Representative immunohistochemistry slides of paraffin-embedded GCT of bone tissue specimens for MMP-2 and <t>MMP-9</t>
    Type Iv Collagenase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/type iv collagenase/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    type iv collagenase - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Millipore collagenase type iv
    Representative immunohistochemistry slides of paraffin-embedded GCT of bone tissue specimens for MMP-2 and <t>MMP-9</t>
    Collagenase Type Iv, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase type iv/product/Millipore
    Average 99 stars, based on 249 article reviews
    Price from $9.99 to $1999.99
    collagenase type iv - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Representative immunohistochemistry slides of paraffin-embedded GCT of bone tissue specimens for MMP-2 and MMP-9

    Journal: The Open bone journal

    Article Title: Matrix Metalloproteinase Activity in the Stromal Cell of Giant Cell Tumor of Bone

    doi: 10.2174/1876525400901010046

    Figure Lengend Snippet: Representative immunohistochemistry slides of paraffin-embedded GCT of bone tissue specimens for MMP-2 and MMP-9

    Article Snippet: Rao VH, Singh RK, Bridge JA, et al. Regulation of MMP-9 (92 kDa type IV collagenase/gelatinase B) expression in stromal cells of human giant cell tumor of bone.

    Techniques: Immunohistochemistry

    Relative expression of MMP-2 and MMP-9 mRNA from GCT stromal cell cultures based on Real-Time PCR

    Journal: The Open bone journal

    Article Title: Matrix Metalloproteinase Activity in the Stromal Cell of Giant Cell Tumor of Bone

    doi: 10.2174/1876525400901010046

    Figure Lengend Snippet: Relative expression of MMP-2 and MMP-9 mRNA from GCT stromal cell cultures based on Real-Time PCR

    Article Snippet: Rao VH, Singh RK, Bridge JA, et al. Regulation of MMP-9 (92 kDa type IV collagenase/gelatinase B) expression in stromal cells of human giant cell tumor of bone.

    Techniques: Expressing, Real-time Polymerase Chain Reaction