Structured Review

Toyobo coli dh5α strain
Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli <t>DH5α</t> (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P
Coli Dh5α Strain, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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1) Product Images from "Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers"

Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

Journal: PLoS ONE

doi: 10.1371/journal.pone.0169367

Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P
Figure Legend Snippet: Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P

Techniques Used: Mutagenesis, Negative Control

Bacterial adherence to Caco-2 cells for the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), PAO1Tn:: flgE mutant ( ΔflgE ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (20, 30, 40, and 50 mM). Bacterial adherence was determined based on the number of adhered bacteria per Caco-2 cell. The assay was performed in six replicates, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P
Figure Legend Snippet: Bacterial adherence to Caco-2 cells for the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), PAO1Tn:: flgE mutant ( ΔflgE ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (20, 30, 40, and 50 mM). Bacterial adherence was determined based on the number of adhered bacteria per Caco-2 cell. The assay was performed in six replicates, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

Techniques Used: Mutagenesis, Negative Control

Penetration activity of P . aeruginosa strains. (A) The wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), and PAO1Tn:: flgE mutant ( ΔflgE ) were inoculated onto the apical surfaces of Caco-2 cell monolayers at an MOI of 100, and the number of bacteria in the basolateral medium was counted at 6 h after infection. The assay was performed in triplicate, and the results are expressed as the mean ± SD. E . coli DH5α was used as a negative control. *#: P
Figure Legend Snippet: Penetration activity of P . aeruginosa strains. (A) The wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), and PAO1Tn:: flgE mutant ( ΔflgE ) were inoculated onto the apical surfaces of Caco-2 cell monolayers at an MOI of 100, and the number of bacteria in the basolateral medium was counted at 6 h after infection. The assay was performed in triplicate, and the results are expressed as the mean ± SD. E . coli DH5α was used as a negative control. *#: P

Techniques Used: Activity Assay, Mutagenesis, Infection, Negative Control

PGDH activity assay. (A) SDS-PAGE analysis to verify overexpression of the P . aeruginosa serA and synthesis of SerA proteins in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The red arrow indicates an overexpressed band at the expected size of 44 kDa for P . aeruginosa SerA. (B) Inhibitory effect of 50 mM L -serine on PGDH activity of SerA proteins (mU/mg) in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The assay was performed in five replicates, and the results are expressed as the mean ± SD. Significant differences were observed between SerA and mock (*: P
Figure Legend Snippet: PGDH activity assay. (A) SDS-PAGE analysis to verify overexpression of the P . aeruginosa serA and synthesis of SerA proteins in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The red arrow indicates an overexpressed band at the expected size of 44 kDa for P . aeruginosa SerA. (B) Inhibitory effect of 50 mM L -serine on PGDH activity of SerA proteins (mU/mg) in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The assay was performed in five replicates, and the results are expressed as the mean ± SD. Significant differences were observed between SerA and mock (*: P

Techniques Used: Activity Assay, SDS Page, Over Expression, Isolation

Motility assays. (A) Swimming motility of WT, ΔserA , + serA , and E . coli DH5α (as a negative control). A representative image from the swimming motility assay is shown. The major axis of swimming is the longest length of the swimming area. The assay was performed in triplicate, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P
Figure Legend Snippet: Motility assays. (A) Swimming motility of WT, ΔserA , + serA , and E . coli DH5α (as a negative control). A representative image from the swimming motility assay is shown. The major axis of swimming is the longest length of the swimming area. The assay was performed in triplicate, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

Techniques Used: Negative Control, Motility Assay

Related Articles

Transformation Assay:

Article Title: Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis
Article Snippet: .. After ligation, competent cells of E . coli DH5α strains (Competent high DH5α, Toyobo, Tokyo, Japan) were transformed with the above plasmid. .. Transformants were selected on LB agar plates containing 50 μg/ml kanamycin, and the plasmid DNAs were purified using NucleoBond PC 100 columns (Macherey-Nagel GmbH & Co., Doren, Germany).

Plasmid Preparation:

Article Title: Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis
Article Snippet: .. After ligation, competent cells of E . coli DH5α strains (Competent high DH5α, Toyobo, Tokyo, Japan) were transformed with the above plasmid. .. Transformants were selected on LB agar plates containing 50 μg/ml kanamycin, and the plasmid DNAs were purified using NucleoBond PC 100 columns (Macherey-Nagel GmbH & Co., Doren, Germany).

Ligation:

Article Title: Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis
Article Snippet: .. After ligation, competent cells of E . coli DH5α strains (Competent high DH5α, Toyobo, Tokyo, Japan) were transformed with the above plasmid. .. Transformants were selected on LB agar plates containing 50 μg/ml kanamycin, and the plasmid DNAs were purified using NucleoBond PC 100 columns (Macherey-Nagel GmbH & Co., Doren, Germany).

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  • 91
    Toyobo coli dh5α strain
    Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli <t>DH5α</t> (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P
    Coli Dh5α Strain, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli dh5α strain/product/Toyobo
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    coli dh5α strain - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    89
    Toyobo escherichia coli dh5α strain
    Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli <t>DH5α</t> (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P
    Escherichia Coli Dh5α Strain, supplied by Toyobo, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli dh5α strain/product/Toyobo
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli dh5α strain - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    91
    Toyobo e coli strains dh5α
    Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli <t>DH5α</t> (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P
    E Coli Strains Dh5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strains dh5α/product/Toyobo
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli strains dh5α - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Fly survival experiments. Virulence of the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (10, 20, 30, 40, and 50 mM) was evaluated. Significant differences, based on the log-rank test, were observed between WT and DH5α (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Mutagenesis, Negative Control

    Bacterial adherence to Caco-2 cells for the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), PAO1Tn:: flgE mutant ( ΔflgE ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (20, 30, 40, and 50 mM). Bacterial adherence was determined based on the number of adhered bacteria per Caco-2 cell. The assay was performed in six replicates, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Bacterial adherence to Caco-2 cells for the wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), PAO1Tn:: flgE mutant ( ΔflgE ), E . coli DH5α (as a negative control), and WT in the presence of L -serine (20, 30, 40, and 50 mM). Bacterial adherence was determined based on the number of adhered bacteria per Caco-2 cell. The assay was performed in six replicates, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Mutagenesis, Negative Control

    Penetration activity of P . aeruginosa strains. (A) The wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), and PAO1Tn:: flgE mutant ( ΔflgE ) were inoculated onto the apical surfaces of Caco-2 cell monolayers at an MOI of 100, and the number of bacteria in the basolateral medium was counted at 6 h after infection. The assay was performed in triplicate, and the results are expressed as the mean ± SD. E . coli DH5α was used as a negative control. *#: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Penetration activity of P . aeruginosa strains. (A) The wild-type strain (WT), PAO1Tn:: serA mutant ( ΔserA ), PAO1Tn:: serA (pUCP19- serA ) complementary strain (+ serA ), and PAO1Tn:: flgE mutant ( ΔflgE ) were inoculated onto the apical surfaces of Caco-2 cell monolayers at an MOI of 100, and the number of bacteria in the basolateral medium was counted at 6 h after infection. The assay was performed in triplicate, and the results are expressed as the mean ± SD. E . coli DH5α was used as a negative control. *#: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Activity Assay, Mutagenesis, Infection, Negative Control

    PGDH activity assay. (A) SDS-PAGE analysis to verify overexpression of the P . aeruginosa serA and synthesis of SerA proteins in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The red arrow indicates an overexpressed band at the expected size of 44 kDa for P . aeruginosa SerA. (B) Inhibitory effect of 50 mM L -serine on PGDH activity of SerA proteins (mU/mg) in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The assay was performed in five replicates, and the results are expressed as the mean ± SD. Significant differences were observed between SerA and mock (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: PGDH activity assay. (A) SDS-PAGE analysis to verify overexpression of the P . aeruginosa serA and synthesis of SerA proteins in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The red arrow indicates an overexpressed band at the expected size of 44 kDa for P . aeruginosa SerA. (B) Inhibitory effect of 50 mM L -serine on PGDH activity of SerA proteins (mU/mg) in crude extract isolated from DH5α (ptac-85- serA ) culture (SerA) and from DH5α (ptac-85) culture (mock). The assay was performed in five replicates, and the results are expressed as the mean ± SD. Significant differences were observed between SerA and mock (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Activity Assay, SDS Page, Over Expression, Isolation

    Motility assays. (A) Swimming motility of WT, ΔserA , + serA , and E . coli DH5α (as a negative control). A representative image from the swimming motility assay is shown. The major axis of swimming is the longest length of the swimming area. The assay was performed in triplicate, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Journal: PLoS ONE

    Article Title: Pseudomonas aeruginosa serA Gene Is Required for Bacterial Translocation through Caco-2 Cell Monolayers

    doi: 10.1371/journal.pone.0169367

    Figure Lengend Snippet: Motility assays. (A) Swimming motility of WT, ΔserA , + serA , and E . coli DH5α (as a negative control). A representative image from the swimming motility assay is shown. The major axis of swimming is the longest length of the swimming area. The assay was performed in triplicate, and the results are expressed as the mean ± SD. Significant differences were observed between WT and ΔserA (*: P

    Article Snippet: E . coli DH5α strain was used as a control strain that does not penetrate epithelial cell monolayers [ ] and was purchased from TOYOBO, Japan.

    Techniques: Negative Control, Motility Assay