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anti col 3  (Proteintech)


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    Structured Review

    Proteintech anti col 3
    Anti Col 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/col+3/Collagen+Type+III+(N-terminal)+Polyclonal+antibody/10__1093_slash_burnst_slash_tkag072-109-38-40
    Average 96 stars, based on 623 article reviews
    anti col 3 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Exosomes from human adipose-derived mesenchymal stem cells inhibit production of extracellular matrix in keloid fibroblasts via downregulating transforming growth factor-β2 and Notch-1 expression
    Article Snippet: COL-1 , Proteintech, 14,695-1-AP , Rabbit-anti-human , WB. .. COL-3 , Proteintech, 22,734-1-AP , Rabbit-anti-human , WB. .. FN , Proteintech, A12932 , Rabbit-anti-human , WB.

    Incubation:

    Article Title: M2 macrophages promote PKM2 production in fibroblasts to alleviate UVB-induced photoaging
    Article Snippet: .. Cells were then blocked with 5% BSA and incubated overnight at 4°C with primary antibodies against COL-1 (66761–1-Ig, 1:50, Proteintech, China), COL-3 (22734–1-AP, 1:50, Proteintech, China), PKM2 (15822–1-AP, 1:50, Proteintech, China), CCL1 (YP-Ab -06,187, 1:50, Research Cloud Biology, China), CCR8 (A4288, 1:50, ABclonal, China), CD206 (18704–1-AP, 1:50, Proteintech, China), CD86 (13395–1-AP, 1:50, Proteintech, China). .. After washing with PBS, cells were incubated with secondary antibodies (4414S, 1:500, Cell Signaling Technology, USA; 4413S, 1:500, Cell Signaling Technology, USA) at 37°C for 1 h. Finally, cells were stained with DAPI (S2110, Solarbio, China) and imaged using a confocal microscope (Zeiss, Germany).

    Immunofluorescence:

    Article Title: Silk fibroin biomaterial orchestrates soft tissue repair via mitochondrial trafficking in animals and humans
    Article Snippet: For type I and type III collagen quantification, the immunohistochemically stained images (20×) were analyzed by the ImageJ software with the Colour Deconvolution 3.0.3 plugin. .. Paraffin sections (7 μm) or cells were fixed and subjected to immunofluorescence with primary antibodies against CD31 (NOVUS, NB600-562, 1:200 dilution; NOVUS, NB100-2284, 1:200 dilution), TGF-β1 (Proteintech, 21898-1-AP, 1:500 dilution), PI16 (R&D systems, AF4929, 1:25 dilution), CD68 (Invitrogen, MA5-13324, 1:100 dilution), MPO (Abcam, ab9535, 1:50 dilution), SPP1 (R&D, AF808-SP, 1:20 dilution), ITGAV (Abcam, ab179475, 1:500 dilution), ITGB5 (Invitrogen, 14- 0497-82, 1:500 dilution), F4/80 (BioLegend, 123101, 1:200 dilution; Abcam, ab6640, 1:100 dilution), SEC3 (Proteintech, 84007-5-RR, 1:200 dilution), SEC5 (Proteintech, 12751-1-AP, 1:200 dilution), αSMA (Abcam, ab7817, 1:500 dilution), TOMM20 (Abcam, ab283317, 1:500 dilution), COL-1 (Novus, NB600-408, 1:250 dilution) and COL-3 (Proteintech, 22734- 1-AP, 1:500 dilution) overnight at 4◦C. .. This was followed by incubation with Alexa Fluor 488 Goat Anti-Mouse IgG H&L (Abcam, ab150113), Alexa Fluor 546 Goat Anti-Rabbit IgG H&L (Invitrogen, A11035), Alexa Fluor 488 Goat Anti-Rabbit IgG H&L (Invitrogen, A11008), Alexa Fluor 488 Donkey Anti-Goat IgG H&L (Abcam, ab150129), Alexa Fluor 647 Goat AntiRabbit IgG H&L (Invitrogen, A32733), Alexa Fluor 488 Goat Anti-Rat IgG H&L (Abcam, ab150165), and Alexa Fluor 546 Donkey anti-Mouse IgG H+L (Invitrogen, A10036) for 1 h at room temperature.

    Staining:

    Article Title: Mesenchymal Stem Cell-Derived Extracellular Vesicles Embedded in a Self-Adaptive Multifunctional Hydrogel for Rapid Healing of Infected Wounds.
    Article Snippet: Wound healing represents a major clinical concern, disrupted by cellular dysfunction due to infection, metabolic imbalance, and immune response, resulting in physical and economic burden to the patients.. Despite the widespread recognition of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in wound healing research, ensuring their release and retention at the wound site and maximizing their therapeutic efficacy remain significant challenges.. To address these issues, a self-adaptive multifunctional hydrogel is introduced that allows the self-adaptive release of MSC-EVs at the wound sites.



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    Evaluation of COA-SA/CLT-siSnail micelles in treatment of UUO. (A) Representative H&E and Masson staining images of kidney tissue sections from patients with CKD. Scale bars, 50 μm. (B) Representative α -SMA and E-cadherin immunofluorescence staining images from kidney tissue sections of patients with CKD. Scale bars, 100 μm. (C) Flow diagram of treatment of different preparations against UUO-induced renal fibrosis. (D) Cr and BUN levels on Day 14 after treatment. (E) Contralateral renal index (left) and ligation side renal index (right) of UUO mice after treatment. (F) Renal tubular injury score of UUO mice following treatment. (G) The percentage of collagen area in renal tissue section by Masson staining. (H) Average optical density analysis for COL I, COL IV, FN, VIM, α -SMA, E-CAD, and SNAIL immunohistochemical staining. (I) Real-time qPCR analysis of renal mRNA levels of Acta2 , Col1a1 , Col4a1 , Cadh1 , Fn-1 , Vim , and Snai1 in sham and UUO mice on Day 14. GAPDH mRNA as internal control. Data represent mean ± SD ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . UUO; # P < 0.05; ## P < 0.01; ### P < 0.001; #### P < 0.0001 vs . sham. ns, not significant.
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    Evaluation of COA-SA/CLT-siSnail micelles in treatment of UUO. (A) Representative H&E and Masson staining images of kidney tissue sections from patients with CKD. Scale bars, 50 μm. (B) Representative α -SMA and E-cadherin immunofluorescence staining images from kidney tissue sections of patients with CKD. Scale bars, 100 μm. (C) Flow diagram of treatment of different preparations against UUO-induced renal fibrosis. (D) Cr and BUN levels on Day 14 after treatment. (E) Contralateral renal index (left) and ligation side renal index (right) of UUO mice after treatment. (F) Renal tubular injury score of UUO mice following treatment. (G) The percentage of collagen area in renal tissue section by Masson staining. (H) Average optical density analysis for COL I, COL IV, FN, VIM, α -SMA, E-CAD, and SNAIL immunohistochemical staining. (I) Real-time qPCR analysis of renal mRNA levels of Acta2 , Col1a1 , Col4a1 , Cadh1 , Fn-1 , Vim , and Snai1 in sham and UUO mice on Day 14. GAPDH mRNA as internal control. Data represent mean ± SD ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . UUO; # P < 0.05; ## P < 0.01; ### P < 0.001; #### P < 0.0001 vs . sham. ns, not significant.
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    PSP inhibits hepatic collagen production in liver fibrosis-induced rats. Detection of the liver fibrosis markers (A) HA, (B) LN, (C) PIIINP and (D) Col IV in serum samples was performed by ELISA. (E) Expression levels of Col I and <t>Col</t> <t>III</t> in the liver were detected by immunohistochemistry; scale bar, 20 µm. Semi-quantification of (F) Col I and (G) Col III. ELISA was used to detect the levels of (H) Col I and (I) Col III in liver tissue. (J) Levels of Hyp in the liver tissues were detected by ELISA. Data are presented as the mean ± standard deviation, n=6. ### P<0.001 vs. the control group; *P<0.05, **P<0.01 and ***P<0.001 vs. the model group. BJRG, Biejia ruangan; Col, collagen; H, high; HA, hyaluronic acid; Hyp, hydroxyproline; L, low; LN, laminin; PIIINP, procollagen III N-terminal peptide; PSP, Polygonatum sibiricum polysaccharide.
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    Image Search Results


    Evaluation of COA-SA/CLT-siSnail micelles in treatment of UUO. (A) Representative H&E and Masson staining images of kidney tissue sections from patients with CKD. Scale bars, 50 μm. (B) Representative α -SMA and E-cadherin immunofluorescence staining images from kidney tissue sections of patients with CKD. Scale bars, 100 μm. (C) Flow diagram of treatment of different preparations against UUO-induced renal fibrosis. (D) Cr and BUN levels on Day 14 after treatment. (E) Contralateral renal index (left) and ligation side renal index (right) of UUO mice after treatment. (F) Renal tubular injury score of UUO mice following treatment. (G) The percentage of collagen area in renal tissue section by Masson staining. (H) Average optical density analysis for COL I, COL IV, FN, VIM, α -SMA, E-CAD, and SNAIL immunohistochemical staining. (I) Real-time qPCR analysis of renal mRNA levels of Acta2 , Col1a1 , Col4a1 , Cadh1 , Fn-1 , Vim , and Snai1 in sham and UUO mice on Day 14. GAPDH mRNA as internal control. Data represent mean ± SD ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . UUO; # P < 0.05; ## P < 0.01; ### P < 0.001; #### P < 0.0001 vs . sham. ns, not significant.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Targeting renal tubular epithelial cells via a dual functionalized oligosaccharide self-assembly in the management of acute and chronic kidney diseases

    doi: 10.1016/j.apsb.2025.09.017

    Figure Lengend Snippet: Evaluation of COA-SA/CLT-siSnail micelles in treatment of UUO. (A) Representative H&E and Masson staining images of kidney tissue sections from patients with CKD. Scale bars, 50 μm. (B) Representative α -SMA and E-cadherin immunofluorescence staining images from kidney tissue sections of patients with CKD. Scale bars, 100 μm. (C) Flow diagram of treatment of different preparations against UUO-induced renal fibrosis. (D) Cr and BUN levels on Day 14 after treatment. (E) Contralateral renal index (left) and ligation side renal index (right) of UUO mice after treatment. (F) Renal tubular injury score of UUO mice following treatment. (G) The percentage of collagen area in renal tissue section by Masson staining. (H) Average optical density analysis for COL I, COL IV, FN, VIM, α -SMA, E-CAD, and SNAIL immunohistochemical staining. (I) Real-time qPCR analysis of renal mRNA levels of Acta2 , Col1a1 , Col4a1 , Cadh1 , Fn-1 , Vim , and Snai1 in sham and UUO mice on Day 14. GAPDH mRNA as internal control. Data represent mean ± SD ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . UUO; # P < 0.05; ## P < 0.01; ### P < 0.001; #### P < 0.0001 vs . sham. ns, not significant.

    Article Snippet: Anti-collagen I (COL I) antibody (GB11022-3), anti- α -SMA antibody (GB13044), anti-Vimentin antibody (GB11192), and anti-Fibronectin (FN) antibody ( GB114057 ) were purchased from Servicebio (Wuhan, China).

    Techniques: Staining, Immunofluorescence, Ligation, Immunohistochemical staining, Control

    Evaluation of COA-SA/CLT-siSnail micelles in treatment of FA-CKD. (A) Flow diagram of the treatment of free CLT solution, COA-SA/CLT micelles, free siSnail solution, COA-SA/siSnail micelles, COA-SA/CLT-siSnail micelles, and COA-SA/CLT-siNC micelles against folate-induced renal fibrosis. (B) Renal index of FA-CKD mice after treatment. (C) Cr and BUN levels on Day 30 after treatment. (D) Renal tubular injury score of FA-CKD mice following treatment. (E) The percentage of collagen area in renal tissue section by Masson staining. (F) Average optical density analysis for COL I, COL IV, FN, VIM, α -SMA, E-CAD, and SNAIL immunohistochemical staining. (G) Real-time qPCR analysis of renal mRNA levels of Acta2 , Col1a1 , Col4a1 , Cadh1 , Fn-1 , Vim , and Snai1 in FA-CKD mice on Day 30 after treatment. GAPDH mRNA as internal control. Data represent mean ± SD ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . FA-CKD; # P < 0.05; ## P < 0.01 vs . vehicle. ns, not significant.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Targeting renal tubular epithelial cells via a dual functionalized oligosaccharide self-assembly in the management of acute and chronic kidney diseases

    doi: 10.1016/j.apsb.2025.09.017

    Figure Lengend Snippet: Evaluation of COA-SA/CLT-siSnail micelles in treatment of FA-CKD. (A) Flow diagram of the treatment of free CLT solution, COA-SA/CLT micelles, free siSnail solution, COA-SA/siSnail micelles, COA-SA/CLT-siSnail micelles, and COA-SA/CLT-siNC micelles against folate-induced renal fibrosis. (B) Renal index of FA-CKD mice after treatment. (C) Cr and BUN levels on Day 30 after treatment. (D) Renal tubular injury score of FA-CKD mice following treatment. (E) The percentage of collagen area in renal tissue section by Masson staining. (F) Average optical density analysis for COL I, COL IV, FN, VIM, α -SMA, E-CAD, and SNAIL immunohistochemical staining. (G) Real-time qPCR analysis of renal mRNA levels of Acta2 , Col1a1 , Col4a1 , Cadh1 , Fn-1 , Vim , and Snai1 in FA-CKD mice on Day 30 after treatment. GAPDH mRNA as internal control. Data represent mean ± SD ( n = 5). ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001 vs . FA-CKD; # P < 0.05; ## P < 0.01 vs . vehicle. ns, not significant.

    Article Snippet: Anti-collagen I (COL I) antibody (GB11022-3), anti- α -SMA antibody (GB13044), anti-Vimentin antibody (GB11192), and anti-Fibronectin (FN) antibody ( GB114057 ) were purchased from Servicebio (Wuhan, China).

    Techniques: Staining, Immunohistochemical staining, Control

    PSP inhibits hepatic collagen production in liver fibrosis-induced rats. Detection of the liver fibrosis markers (A) HA, (B) LN, (C) PIIINP and (D) Col IV in serum samples was performed by ELISA. (E) Expression levels of Col I and Col III in the liver were detected by immunohistochemistry; scale bar, 20 µm. Semi-quantification of (F) Col I and (G) Col III. ELISA was used to detect the levels of (H) Col I and (I) Col III in liver tissue. (J) Levels of Hyp in the liver tissues were detected by ELISA. Data are presented as the mean ± standard deviation, n=6. ### P<0.001 vs. the control group; *P<0.05, **P<0.01 and ***P<0.001 vs. the model group. BJRG, Biejia ruangan; Col, collagen; H, high; HA, hyaluronic acid; Hyp, hydroxyproline; L, low; LN, laminin; PIIINP, procollagen III N-terminal peptide; PSP, Polygonatum sibiricum polysaccharide.

    Journal: Molecular Medicine Reports

    Article Title: Polygonatum sibiricum polysaccharide alleviates liver fibrosis through the TGF-β/Smad signaling pathway and reduces collagen

    doi: 10.3892/mmr.2025.13599

    Figure Lengend Snippet: PSP inhibits hepatic collagen production in liver fibrosis-induced rats. Detection of the liver fibrosis markers (A) HA, (B) LN, (C) PIIINP and (D) Col IV in serum samples was performed by ELISA. (E) Expression levels of Col I and Col III in the liver were detected by immunohistochemistry; scale bar, 20 µm. Semi-quantification of (F) Col I and (G) Col III. ELISA was used to detect the levels of (H) Col I and (I) Col III in liver tissue. (J) Levels of Hyp in the liver tissues were detected by ELISA. Data are presented as the mean ± standard deviation, n=6. ### P<0.001 vs. the control group; *P<0.05, **P<0.01 and ***P<0.001 vs. the model group. BJRG, Biejia ruangan; Col, collagen; H, high; HA, hyaluronic acid; Hyp, hydroxyproline; L, low; LN, laminin; PIIINP, procollagen III N-terminal peptide; PSP, Polygonatum sibiricum polysaccharide.

    Article Snippet: Goat serum (cat. no. c-0005), MMP2 (cat. no. bs4605R), MMP9 (cat. no. bsm55544m), Col III (cat no. bs-0549R) and Col I (cat no. bs-7158R) antibodies were obtained from Bioss.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Standard Deviation, Control