cobas integra 800 system  (Roche)

Journal: Italian Journal of Pediatrics

Article Title: Plant sterols-enriched diet decreases small, dense LDL-cholesterol levels in children with hypercholesterolemia: a prospective study

doi: 10.1186/1824-7288-40-42

Figure Lengend Snippet: Distribution of sdLDL-C in controls and in hypercholesterolemic children.

Article Snippet: A commercially available kit (sLDL-EX “SEIKEN”, Randox Laboratories Ltd, UK) was used for the direct quantitative determination of sdLDL-C (Roche Diagnostics, Cobas Integra 800).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

doi: 10.2147/IJN.S31030

Figure Lengend Snippet: Correlation between CRP concentrations in human serum measured via IMR (denoted as CRP+) and immunoturbidimetry (denoted as CRP*). Abbreviations: CRP, C-reactive protein; IMR, immunomagnetic reduction.

Article Snippet: Thus, the correlation between assaying CRP using IMR (XacPro-E101, MagQu Co, Ltd, Sindian District, New Taipei City) and immunoturbidimetry (Cobas Integra 800, Roche) was clarified.

Techniques:

Journal: Molecular Cancer

Article Title: Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake

doi: 10.1186/1476-4598-6-55

Figure Lengend Snippet: Reduction of intracellular iron levels by sodium ascorbate . 30 × 10 6 of either HTLA-230 or SH-SY5Y were treated with the indicated doses of sodium ascorbate for 24 hours and iron levels were measured in cell lysates. After terminating the incubation, cells were collected by scraping and washed three times with PBS and then lysed in specific buffer. Iron levels were analyzed with a Cobas Integra 800 system as described under "Material and Method". Values indicated are the mean ± S.D. of three separate experiments.

Article Snippet: Iron concentration in cell lysates was performed with a Cobas Integra 800 system (Roche Diagnostics GmbH, Mannheim, Germany) as routinarily assessed on plasma or serum samples by an Iron Test (Roche Diagnostics); iron concentration was estimated by absorbance at 552 nm.

Techniques: Incubation

Journal: Frontiers in Immunology

Article Title: The Long Pentraxin PTX3 Is an Endogenous Inhibitor of Hyperoxaluria-Related Nephrocalcinosis and Chronic Kidney Disease

doi: 10.3389/fimmu.2018.02173

Figure Lengend Snippet: Development of nephrocalcinosis and CKD under hyperoxaluric conditions is strain and sex dependent. Male and female BALB/c, C57BL/6N, and CD-1 mice (8 weeks of age) were fed with control or oxalate chow for 3 weeks ( n = 5). (A) Quantification of calcium oxalate crystal deposition was conducted using Pizzolato's method on formalin fixed kidney sections as shown in (B) . (C) Quantification of tubular injury was conducted using PAS-stained formalin fixed kidney sections as shown in (D) . (E) Quantification of Collagen I deposition was conducted using Collagen I-stained formalin fixed kidney sections as shown in (F) . (G) Plasma creatinine, (H) blood urea nitrogen, and (I) plasma phosphorus were analyzed Cobas Integra 800 autoanalyzer. n.s., not significant; * p

Article Snippet: Plasma BUN, creatinine and phosphorus levels were measured using a Cobas Integra 800 autoanalyzer (Roche, Mannheim, Germany).

Techniques: Mouse Assay, Staining