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Sanyo co2 incubator mco 18aic
Co2 Incubator Mco 18aic, supplied by Sanyo, used in various techniques. Bioz Stars score: 81/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/co2 incubator mco 18aic/product/Sanyo
Average 81 stars, based on 131 article reviews
Price from $9.99 to $1999.99
co2 incubator mco 18aic - by Bioz Stars, 2020-01
81/100 stars

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MTT Assay:

Article Title: Antioxidant and Neuroprotective Effects of Doenjang Prepared with Rhizopus, Pichia, and Bacillus
Article Snippet: Neuroprotective activity assay HT22 mouse hippocampal neuronal cells were routinely maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS and 100 units/mL penicillin and 100 μg/mL streptomycin in a humidified CO2 incubator (MCO-19-AIC, Sanyo, Osaka, Japan) at 37°C and 5% CO2 /95% air. .. After culturing cells for 24 h, the cell survival rate was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay ( ).

Fluorescence:

Article Title: Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy
Article Snippet: CO2 cell culture incubator (SANYO, cat. no. MCO-36AIC) set up at 37°C. .. Fluorescence microscope (Leica Microsystems Inc., Exton, PA, model no. DMIL) equipped with a GFP filter cube (Excitation BP 450–490 nm, Dichroic RSP 510 nm, Emission LP 515 nm).

Concentration Assay:

Article Title: Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L
Article Snippet: Upon autopsy, glucose uptake of isolated diaphragms was assessed after immersing them in 1 g/L glucose solution and glucose concentration was measured prior and 30 min after incubation ( ). .. Briefly, subsequent to chronic treatment, on day 31, animals were scarified and diaphragms were removed and washed immediately with krebs-ringer solution and were immersed in 2% bovine serum albumin supplemented with tyrode ringer solution buffer containing 1 g/L glucose solution and incubated in a CO2 incubator (Sanyo Electric Co. Ltd., Tokyo, Japan) at 37 °C, 95% air for 30 min.

Article Title: Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities
Article Snippet: Antitumor Activity Evaluation HepG2 cells or SGC7901 cells in the logarithmic phase were adjusted to 4 × 104 cells/mL by Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum, and then plated in 96-well culture plates (100 μL/well) for 6 h incubation at 37 °C in a humidified 5% CO2 incubator (MCO-17 AIC, SANYO, Tokyo, Japan). .. After the removal of culture supernatant, a 200 μL/well medium containing a certain concentration of LPPMs (0, 50, 100, 200, 400 or 800 μg/mL) was then added in for 24 h incubation.

Multiple Displacement Amplification:

Article Title: Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis
Article Snippet: The human breast cancer cell line MDA-MB-231 (92020424) was obtained from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK). .. Cells were incubated in a CO2 incubator (MCO-19AIC, SANYO, Osaka, Japan) at 37 °C in a humidified atmosphere containing 5% CO2 .

Isolation:

Article Title: Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L
Article Snippet: Upon autopsy, glucose uptake of isolated diaphragms was assessed after immersing them in 1 g/L glucose solution and glucose concentration was measured prior and 30 min after incubation ( ). .. Briefly, subsequent to chronic treatment, on day 31, animals were scarified and diaphragms were removed and washed immediately with krebs-ringer solution and were immersed in 2% bovine serum albumin supplemented with tyrode ringer solution buffer containing 1 g/L glucose solution and incubated in a CO2 incubator (Sanyo Electric Co. Ltd., Tokyo, Japan) at 37 °C, 95% air for 30 min.

Article Title: Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions
Article Snippet: .. The isolated cells were then divided into equal parts and permanently exposed to two oxygen conditions: hyperoxia (21% O2 , 74% N2 , and 5% CO2 ) in a CO2 incubator (Sanyo; ESBE Scientific, St.-Laurent, Canada) and low physiologic oxygen conditions (3% O2 , 92% N2 , and 5% CO2 ) in a multigas incubator (Sanyo; ESBE Scientific). .. Choroidal melanocytes (early passages, P2–P6) were grown for all experiments using FNC Coating Mix (AthenaES, Baltimore, MD), and Ham’s F12 medium supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT), 10 ng/ml cholera toxin (Sigma-Aldrich, St. Louis, MO), 100 nM phorbol 12-myristate 13-acetate (Sigma-Aldrich), 100 µg/ml geneticin (Wisent, Quebec City, QC), and 50 µg/ml gentamicin [ , ].

Article Title: Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension
Article Snippet: Paragraph title: Chondrocyte harvest, isolation, and expansion ... Chondrocytes were split from each prep at the end of primary (P0) and first passage (P1) into two equal groups and further expanded at 20% or 5% O2 using a CO2 /N2 incubator (MCO-18AIC; Sanyo, Tokyo, Japan).

Article Title: Mesenchymal Stem Cells in Restoration of Fertility at Experimental Pelvic Inflammatory Disease
Article Snippet: Paragraph title: 2.2. Isolation, Culture, and Cryopreservation of MSCs ... We used standard culture conditions (37°C, 5% CO2 , and 95% humidity) in a CO2 incubator (Sanyo, Osaka, Japan).

CCK-8 Assay:

Article Title: Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities
Article Snippet: Antitumor Activity Evaluation HepG2 cells or SGC7901 cells in the logarithmic phase were adjusted to 4 × 104 cells/mL by Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum, and then plated in 96-well culture plates (100 μL/well) for 6 h incubation at 37 °C in a humidified 5% CO2 incubator (MCO-17 AIC, SANYO, Tokyo, Japan). .. The proliferation of tumor cells was detected by a CCK-8 kit according to its instruction.

Microscopy:

Article Title: Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy
Article Snippet: CO2 cell culture incubator (SANYO, cat. no. MCO-36AIC) set up at 37°C. .. Fluorescence microscope (Leica Microsystems Inc., Exton, PA, model no. DMIL) equipped with a GFP filter cube (Excitation BP 450–490 nm, Dichroic RSP 510 nm, Emission LP 515 nm).

Mouse Assay:

Article Title: Mesenchymal Stem Cells in Restoration of Fertility at Experimental Pelvic Inflammatory Disease
Article Snippet: Isolation, Culture, and Cryopreservation of MSCs MSCs were isolated from resected femur of mice by washing out with Hanks' solution (PAA, Pasching, Austria) followed by flushing through needles with gradually decreased diameter. .. We used standard culture conditions (37°C, 5% CO2 , and 95% humidity) in a CO2 incubator (Sanyo, Osaka, Japan).

Article Title: Hepcidin is regulated by promoter-associated histone acetylation and HDAC3
Article Snippet: Precision cut liver slices PCLS were cultured up to 24 h in 12-well plates with William E + Glutamax (Gibco, Grand Island, New York, USA), supplemented with 14 mM d -Glucose (Merck, Darmstadt, Germany) and 50 µg/ml gentamicin (Gibco, Grand Island, New York, USA) at 37 °C in an O2 /CO2 incubator (MCO-18M, Sanyo, USA), which was continuously shaken at a speed of 90 rpm and saturated with 80% O2 and 5% CO2 . .. Experiments using mice as a source for precision cut liver slices were approved by the Animal Ethical Committee of the University of Groningen, Netherlands .

Passaging:

Article Title: Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension
Article Snippet: Chondrocytes were split from each prep at the end of primary (P0) and first passage (P1) into two equal groups and further expanded at 20% or 5% O2 using a CO2 /N2 incubator (MCO-18AIC; Sanyo, Tokyo, Japan). .. Medium changes, passaging, and the preparation for aggregate and engineered cartilage, were performed under standard O2 conditions as previously described ( ).

Incubation:

Article Title: Involvement of apoptotic pathways in docosahexaenoic acid-induced benefit in prostate cancer: Pathway-focused gene expression analysis using RT2 Profile PCR Array System
Article Snippet: .. Cells were incubated in a 5% CO2 incubator (Sanyo Electric Biomedical CO., LTD., Japan) at 37 °C. .. Fish oil (Shanghai Haling biotechnology Co., Ltd., Shanghai, China), DHA, eicosapentaenoic acid (EPA), and arachidonic acid (AA, 50 mmol/L, Sigma) were prepared using NaOH and were dissolved in BSA (0.01 mol/L PBS, 1:9).

Article Title: Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L
Article Snippet: .. Briefly, subsequent to chronic treatment, on day 31, animals were scarified and diaphragms were removed and washed immediately with krebs-ringer solution and were immersed in 2% bovine serum albumin supplemented with tyrode ringer solution buffer containing 1 g/L glucose solution and incubated in a CO2 incubator (Sanyo Electric Co. Ltd., Tokyo, Japan) at 37 °C, 95% air for 30 min. .. Following incubation, each diaphragm was removed from incubation media and 10 µL of the media was removed to assess the glucose concentration using glucose oxidation kit and a spectrophotometer at 500 nm.

Article Title: Anti-tumor activity of safranal against neuroblastoma cells
Article Snippet: .. The cells were incubated in a humidified 5% CO2 and 95% air atmosphere at 37°C in CO2 incubator MCO-17AI (Sanyo Electric Co., Ltd., Japan). ..

Article Title: Bioluminescence Imaging to Track Bacteroides fragilis Inhibition of Vibrio parahaemolyticus Infection in Mice
Article Snippet: Both cell lines were cultured under a moist atmosphere in a 5% CO2 incubator (MCO-18AIC SANYO, Japan) at 37°C. .. A 300-μl volume of cell suspension (2 × 105 cells/ml) was added to each well, and plates were incubated in a 5% CO2 incubator at 37°C for 12–14 h. Following incubation, the culture medium was aspirated from all wells.

Article Title: Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities
Article Snippet: .. Antitumor Activity Evaluation HepG2 cells or SGC7901 cells in the logarithmic phase were adjusted to 4 × 104 cells/mL by Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum, and then plated in 96-well culture plates (100 μL/well) for 6 h incubation at 37 °C in a humidified 5% CO2 incubator (MCO-17 AIC, SANYO, Tokyo, Japan). .. After the removal of culture supernatant, a 200 μL/well medium containing a certain concentration of LPPMs (0, 50, 100, 200, 400 or 800 μg/mL) was then added in for 24 h incubation.

Article Title: Normothermic Microwave Irradiation Induces Death of HL-60 Cells through Heat-Independent Apoptosis
Article Snippet: .. Cells were incubated in a CO2 incubator (MCO-19AIC, SANYO, Osaka, Japan) at 37 °C in a humidified atmosphere containing 5% CO2 . .. Novel microwave irradiation system The novel microwave irradiation system used here, which incorporates a semiconductor microwave oscillator and applicator, was developed in cooperation with Sunny Engineering Co., Ltd. (MTS03(S), Osaka, Japan, Fig. S ) .

Inhibition:

Article Title: Bioluminescence Imaging to Track Bacteroides fragilis Inhibition of Vibrio parahaemolyticus Infection in Mice
Article Snippet: Paragraph title: RTCA pathogen inhibition assay ... Both cell lines were cultured under a moist atmosphere in a 5% CO2 incubator (MCO-18AIC SANYO, Japan) at 37°C.

Proliferation Assay:

Article Title: Antioxidant and Neuroprotective Effects of Doenjang Prepared with Rhizopus, Pichia, and Bacillus
Article Snippet: Neuroprotective activity assay HT22 mouse hippocampal neuronal cells were routinely maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS and 100 units/mL penicillin and 100 μg/mL streptomycin in a humidified CO2 incubator (MCO-19-AIC, Sanyo, Osaka, Japan) at 37°C and 5% CO2 /95% air. .. After culturing cells for 24 h, the cell survival rate was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay ( ).

Cell Culture:

Article Title: Involvement of apoptotic pathways in docosahexaenoic acid-induced benefit in prostate cancer: Pathway-focused gene expression analysis using RT2 Profile PCR Array System
Article Snippet: Paragraph title: Cell culture and treatment ... Cells were incubated in a 5% CO2 incubator (Sanyo Electric Biomedical CO., LTD., Japan) at 37 °C.

Article Title: Genetic profiling of young and aged endothelial progenitor cells in hypoxia
Article Snippet: .. EPCs were cultured in a CO2 Cell Culture Incubator (SANYO MCO-5AC, San Diego, USA) under atmospheric oxygen (20% O2 ) and hypoxic conditions (1% O2 ) for designated durations. .. Cells were cultured in 24-well plates and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (SI-M5655, Sigma-Aldrich, St. Louis, MO) was added to the medium to a final concentration of 0.5 mg/mL.

Article Title: Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions
Article Snippet: Paragraph title: Human choroidal melanocyte cell culture ... The isolated cells were then divided into equal parts and permanently exposed to two oxygen conditions: hyperoxia (21% O2 , 74% N2 , and 5% CO2 ) in a CO2 incubator (Sanyo; ESBE Scientific, St.-Laurent, Canada) and low physiologic oxygen conditions (3% O2 , 92% N2 , and 5% CO2 ) in a multigas incubator (Sanyo; ESBE Scientific).

Article Title: Anti-tumor activity of safranal against neuroblastoma cells
Article Snippet: Paragraph title: Cell culture ... The cells were incubated in a humidified 5% CO2 and 95% air atmosphere at 37°C in CO2 incubator MCO-17AI (Sanyo Electric Co., Ltd., Japan).

Article Title: Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension
Article Snippet: Chondrocytes were isolated from these tissues as previously described ( , ), plated at 5.7 × 103 cells/cm2 in T-175 cell culture flasks (431080; Corning, Lowell, MA) and grown in expansion medium (Dulbecco’s modified Eagle’s medium [DMEM] with 1g/L glucose (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum [FBS, Invitrogen; lot# 281497]) under standard conditions (humidified atmosphere, 37°C, 20% O2 , 5% CO2 ). .. Chondrocytes were split from each prep at the end of primary (P0) and first passage (P1) into two equal groups and further expanded at 20% or 5% O2 using a CO2 /N2 incubator (MCO-18AIC; Sanyo, Tokyo, Japan).

Article Title: Bioluminescence Imaging to Track Bacteroides fragilis Inhibition of Vibrio parahaemolyticus Infection in Mice
Article Snippet: .. Both cell lines were cultured under a moist atmosphere in a 5% CO2 incubator (MCO-18AIC SANYO, Japan) at 37°C. ..

Article Title: Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy
Article Snippet: .. CO2 cell culture incubator (SANYO, cat. no. MCO-36AIC) set up at 37°C. .. Tissue culture hood biosafety cabinet (The Baker Company, SterilGARD III, cat. no. SG-403a).

Article Title: Attenuation of oxygen fluctuation-induced endoplasmic reticulum stress in human lens epithelial cells
Article Snippet: Some were cultured in glucose-free (GF) DMEM supplemented with 2% FCS and were maintained at 37°C in 20% atmospheric O2 . .. Atmospheric O2 environments of 1 and 4% were maintained in an O2 /CO2 incubator (SANYO MCO-19M CO2 incubator; Sanyo, Tokyo, Japan) attached to a liquid nitrogen gas tank.

Article Title: Hepcidin is regulated by promoter-associated histone acetylation and HDAC3
Article Snippet: .. Precision cut liver slices PCLS were cultured up to 24 h in 12-well plates with William E + Glutamax (Gibco, Grand Island, New York, USA), supplemented with 14 mM d -Glucose (Merck, Darmstadt, Germany) and 50 µg/ml gentamicin (Gibco, Grand Island, New York, USA) at 37 °C in an O2 /CO2 incubator (MCO-18M, Sanyo, USA), which was continuously shaken at a speed of 90 rpm and saturated with 80% O2 and 5% CO2 . ..

Western Blot:

Article Title: Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension
Article Snippet: Native ear (auricular) and humeral head (articular) cartilage tissues were harvested from adult, 12–16 month-old, male New Zealand White rabbits in accordance with the guidelines of the ACUC of Case Western Reserve University and the University of Washington. .. Chondrocytes were split from each prep at the end of primary (P0) and first passage (P1) into two equal groups and further expanded at 20% or 5% O2 using a CO2 /N2 incubator (MCO-18AIC; Sanyo, Tokyo, Japan).

Modification:

Article Title: Involvement of apoptotic pathways in docosahexaenoic acid-induced benefit in prostate cancer: Pathway-focused gene expression analysis using RT2 Profile PCR Array System
Article Snippet: DU145 cells were cultured in Dulbecco’s modified eagle medium (DMEM, Gibico, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Hangzhou Sijiqing Company, China), 100 U/mL penicillin and 0.1 mg/mL streptomycin. .. Cells were incubated in a 5% CO2 incubator (Sanyo Electric Biomedical CO., LTD., Japan) at 37 °C.

Article Title: Anti-tumor activity of safranal against neuroblastoma cells
Article Snippet: The cells were cultured in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetal calf serum (Gibco–Invitrogen), 100 IU·ml-1 penicillin (Gibco–Invitrogen), and 100 μg·ml-1 streptomycin (Gibco–Invitrogen). .. The cells were incubated in a humidified 5% CO2 and 95% air atmosphere at 37°C in CO2 incubator MCO-17AI (Sanyo Electric Co., Ltd., Japan).

Article Title: Disparate Response of Articular- and Auricular-derived Chondrocytes to Oxygen Tension
Article Snippet: Chondrocytes were isolated from these tissues as previously described ( , ), plated at 5.7 × 103 cells/cm2 in T-175 cell culture flasks (431080; Corning, Lowell, MA) and grown in expansion medium (Dulbecco’s modified Eagle’s medium [DMEM] with 1g/L glucose (Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum [FBS, Invitrogen; lot# 281497]) under standard conditions (humidified atmosphere, 37°C, 20% O2 , 5% CO2 ). .. Chondrocytes were split from each prep at the end of primary (P0) and first passage (P1) into two equal groups and further expanded at 20% or 5% O2 using a CO2 /N2 incubator (MCO-18AIC; Sanyo, Tokyo, Japan).

Article Title: Antioxidant and Neuroprotective Effects of Doenjang Prepared with Rhizopus, Pichia, and Bacillus
Article Snippet: .. Neuroprotective activity assay HT22 mouse hippocampal neuronal cells were routinely maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS and 100 units/mL penicillin and 100 μg/mL streptomycin in a humidified CO2 incubator (MCO-19-AIC, Sanyo, Osaka, Japan) at 37°C and 5% CO2 /95% air. ..

Article Title: Bioluminescence Imaging to Track Bacteroides fragilis Inhibition of Vibrio parahaemolyticus Infection in Mice
Article Snippet: LoVo cells were cultured in Dulbecco's Modified Eagle Medium/nutrient mixture F12 (DMEM-H/F12) (1:1) (Gibco, USA) supplemented with 10% fetal bovine serum (MP Biomedicals, USA). .. Both cell lines were cultured under a moist atmosphere in a 5% CO2 incubator (MCO-18AIC SANYO, Japan) at 37°C.

Article Title: Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities
Article Snippet: .. Antitumor Activity Evaluation HepG2 cells or SGC7901 cells in the logarithmic phase were adjusted to 4 × 104 cells/mL by Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum, and then plated in 96-well culture plates (100 μL/well) for 6 h incubation at 37 °C in a humidified 5% CO2 incubator (MCO-17 AIC, SANYO, Tokyo, Japan). .. After the removal of culture supernatant, a 200 μL/well medium containing a certain concentration of LPPMs (0, 50, 100, 200, 400 or 800 μg/mL) was then added in for 24 h incubation.

Article Title: Attenuation of oxygen fluctuation-induced endoplasmic reticulum stress in human lens epithelial cells
Article Snippet: hLECs (Lonza Clonetics™, Basel, Switzerland) were cultured overnight in Dulbecco's modified Eagle's medium (DMEM; Invitrogen Life Technologies, Carlsbad, CA, USA) containing 25 mM glucose, along with 10% fetal calf serum (FCS) under 20% atmospheric O2 at 37°C. .. Atmospheric O2 environments of 1 and 4% were maintained in an O2 /CO2 incubator (SANYO MCO-19M CO2 incubator; Sanyo, Tokyo, Japan) attached to a liquid nitrogen gas tank.

Article Title: Mesenchymal Stem Cells in Restoration of Fertility at Experimental Pelvic Inflammatory Disease
Article Snippet: Cultural medium contained Iscove's Modified Dulbecco's Medium (PAA), 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA), gentamicin (150 mg/ml) (Farmak, Kiev, Ukraine), and amphotericin B (10 mg/ml) (PAA). .. We used standard culture conditions (37°C, 5% CO2 , and 95% humidity) in a CO2 incubator (Sanyo, Osaka, Japan).

Activity Assay:

Article Title: Antioxidant and Neuroprotective Effects of Doenjang Prepared with Rhizopus, Pichia, and Bacillus
Article Snippet: .. Neuroprotective activity assay HT22 mouse hippocampal neuronal cells were routinely maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS and 100 units/mL penicillin and 100 μg/mL streptomycin in a humidified CO2 incubator (MCO-19-AIC, Sanyo, Osaka, Japan) at 37°C and 5% CO2 /95% air. ..

Article Title: Investigation of the Maillard Reaction between Polysaccharides and Proteins from Longan Pulp and the Improvement in Activities
Article Snippet: .. Antitumor Activity Evaluation HepG2 cells or SGC7901 cells in the logarithmic phase were adjusted to 4 × 104 cells/mL by Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum, and then plated in 96-well culture plates (100 μL/well) for 6 h incubation at 37 °C in a humidified 5% CO2 incubator (MCO-17 AIC, SANYO, Tokyo, Japan). .. After the removal of culture supernatant, a 200 μL/well medium containing a certain concentration of LPPMs (0, 50, 100, 200, 400 or 800 μg/mL) was then added in for 24 h incubation.

Immunostaining:

Article Title: Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions
Article Snippet: The isolated cells were then divided into equal parts and permanently exposed to two oxygen conditions: hyperoxia (21% O2 , 74% N2 , and 5% CO2 ) in a CO2 incubator (Sanyo; ESBE Scientific, St.-Laurent, Canada) and low physiologic oxygen conditions (3% O2 , 92% N2 , and 5% CO2 ) in a multigas incubator (Sanyo; ESBE Scientific). .. The morphology and purity of the cultures were confirmed with microscopic inspection in phase contrast and Melan-A immunostaining (MLANA, also known as MART-1; mouse monoclonal, clone A103, prediluted; Dako, Mississauga, Canada).

Spectrophotometry:

Article Title: Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L
Article Snippet: Briefly, subsequent to chronic treatment, on day 31, animals were scarified and diaphragms were removed and washed immediately with krebs-ringer solution and were immersed in 2% bovine serum albumin supplemented with tyrode ringer solution buffer containing 1 g/L glucose solution and incubated in a CO2 incubator (Sanyo Electric Co. Ltd., Tokyo, Japan) at 37 °C, 95% air for 30 min. .. Following incubation, each diaphragm was removed from incubation media and 10 µL of the media was removed to assess the glucose concentration using glucose oxidation kit and a spectrophotometer at 500 nm.

Fluorescence In Situ Hybridization:

Article Title: Involvement of apoptotic pathways in docosahexaenoic acid-induced benefit in prostate cancer: Pathway-focused gene expression analysis using RT2 Profile PCR Array System
Article Snippet: Cells were incubated in a 5% CO2 incubator (Sanyo Electric Biomedical CO., LTD., Japan) at 37 °C. .. Fish oil (Shanghai Haling biotechnology Co., Ltd., Shanghai, China), DHA, eicosapentaenoic acid (EPA), and arachidonic acid (AA, 50 mmol/L, Sigma) were prepared using NaOH and were dissolved in BSA (0.01 mol/L PBS, 1:9).

Derivative Assay:

Article Title: Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions
Article Snippet: The isolated cells were then divided into equal parts and permanently exposed to two oxygen conditions: hyperoxia (21% O2 , 74% N2 , and 5% CO2 ) in a CO2 incubator (Sanyo; ESBE Scientific, St.-Laurent, Canada) and low physiologic oxygen conditions (3% O2 , 92% N2 , and 5% CO2 ) in a multigas incubator (Sanyo; ESBE Scientific). .. Human UM primary cultures (T111, T131, and T132) were derived from ocular tumor biopsies collected at the time of enucleation (Appendix 1; Clinique des tumeurs oculaires du CHU de Québec, Quebec City, Canada).

Hood:

Article Title: Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy
Article Snippet: CO2 cell culture incubator (SANYO, cat. no. MCO-36AIC) set up at 37°C. .. Tissue culture hood biosafety cabinet (The Baker Company, SterilGARD III, cat. no. SG-403a).

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    Sanyo co2 incubator mco 18aic
    Co2 Incubator Mco 18aic, supplied by Sanyo, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/co2 incubator mco 18aic/product/Sanyo
    Average 81 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    co2 incubator mco 18aic - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    78
    Sanyo o2 n2 co2 incubator
    O2 N2 Co2 Incubator, supplied by Sanyo, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/o2 n2 co2 incubator/product/Sanyo
    Average 78 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    o2 n2 co2 incubator - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

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