co2 incubator  (Thermo Fisher)


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    Structured Review

    Thermo Fisher co2 incubator
    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a <t>CO2</t> incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Co2 Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance"

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0032920

    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Figure Legend Snippet: Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Techniques Used: Flow Cytometry, Cytometry, Incubation, Staining

    2) Product Images from "Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance"

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0032920

    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Figure Legend Snippet: Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Techniques Used: Flow Cytometry, Cytometry, Incubation, Staining

    3) Product Images from "Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue"

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue

    Journal: Molecules

    doi: 10.3390/molecules20010863

    BAT3 inhibits endogenous NF-κB target gene expression. L929sA cells left untreated or treated with 25 µM BAT3 for 2 h were subsequently stimulated with 2000 IU/mL TNF for 3 h. Total cytoplasmic RNA was isolated, and converted to cDNA. Corresponding gene expression levels of TNF inducible genes IL6, IL8, A20, COX2 and the constitutively transcribed HPRT (Hypoxanthine-guanine phosphoribosyltransferase 1) were evaluated by Sybr green Q-PCR analysis and were normalized for gene expression of the housekeeping gene β-actin. Statistical significant repression by BAT3 is indicated as not significant ns p > 0.05, * p
    Figure Legend Snippet: BAT3 inhibits endogenous NF-κB target gene expression. L929sA cells left untreated or treated with 25 µM BAT3 for 2 h were subsequently stimulated with 2000 IU/mL TNF for 3 h. Total cytoplasmic RNA was isolated, and converted to cDNA. Corresponding gene expression levels of TNF inducible genes IL6, IL8, A20, COX2 and the constitutively transcribed HPRT (Hypoxanthine-guanine phosphoribosyltransferase 1) were evaluated by Sybr green Q-PCR analysis and were normalized for gene expression of the housekeeping gene β-actin. Statistical significant repression by BAT3 is indicated as not significant ns p > 0.05, * p

    Techniques Used: Expressing, Isolation, SYBR Green Assay, Polymerase Chain Reaction

    4) Product Images from "Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance"

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0032920

    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Figure Legend Snippet: Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Techniques Used: Flow Cytometry, Cytometry, Incubation, Staining

    5) Product Images from "Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate"

    Article Title: Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate

    Journal: In Vivo

    doi: 10.21873/invivo.11575

    Effect of soluble factor from sintering Ti plate containing HA on the proliferation of MC3T3-E1 cells. (A) Sample plates were incubated with culture medium at 37°C for 24 h. The incubation medium was centrifuged at 1700 × g for 10 min, and the supernatant was mixed with cell suspension (final cell density: 4×104 cells/ml). Cells were incubated at 37°C in 5% CO2 for 72 h. (B) Cells prepared by the same method were seeded on the fibronectin-coated culture plate. The proliferation of MC3T3-E1 cells was evaluated by the WST-1 assay. Values are mean±SE for 5 wells. The reproducibility was confirmed by one additional experiment.
    Figure Legend Snippet: Effect of soluble factor from sintering Ti plate containing HA on the proliferation of MC3T3-E1 cells. (A) Sample plates were incubated with culture medium at 37°C for 24 h. The incubation medium was centrifuged at 1700 × g for 10 min, and the supernatant was mixed with cell suspension (final cell density: 4×104 cells/ml). Cells were incubated at 37°C in 5% CO2 for 72 h. (B) Cells prepared by the same method were seeded on the fibronectin-coated culture plate. The proliferation of MC3T3-E1 cells was evaluated by the WST-1 assay. Values are mean±SE for 5 wells. The reproducibility was confirmed by one additional experiment.

    Techniques Used: Incubation, WST-1 Assay

    Effect of O2– on the evaluation of MC3T3-E1 cell proliferation by the WST-1 assay. MC3T3-E1 cells (4×104 cells/ml) were incubated at 37°C in 5% CO2 for 72 h. (A) White column represents the results of the WST-assay in untreated MC3T3-E1. The mean absorbance value of this assay was represented as 100 %. Black column represents the results of the WST-1assay in MC3T3-E1 cells treated with superoxide dismutase (SOD; 300 units/ml). Right columns represent the results of the assay in MC3T3-E1 cells cultured with SOD (300 units/ml). Values are mean±SE for 8 wells. (B) Phase contrast image of cultured MC3T3-E1 cells, showing undistinguishable morphology between a normal culture and the culture added with SOD.
    Figure Legend Snippet: Effect of O2– on the evaluation of MC3T3-E1 cell proliferation by the WST-1 assay. MC3T3-E1 cells (4×104 cells/ml) were incubated at 37°C in 5% CO2 for 72 h. (A) White column represents the results of the WST-assay in untreated MC3T3-E1. The mean absorbance value of this assay was represented as 100 %. Black column represents the results of the WST-1assay in MC3T3-E1 cells treated with superoxide dismutase (SOD; 300 units/ml). Right columns represent the results of the assay in MC3T3-E1 cells cultured with SOD (300 units/ml). Values are mean±SE for 8 wells. (B) Phase contrast image of cultured MC3T3-E1 cells, showing undistinguishable morphology between a normal culture and the culture added with SOD.

    Techniques Used: WST-1 Assay, Incubation, WST Assay, Cell Culture

    6) Product Images from "Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance"

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0032920

    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Figure Legend Snippet: Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Techniques Used: Flow Cytometry, Cytometry, Incubation, Staining

    7) Product Images from "Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance"

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0032920

    Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.
    Figure Legend Snippet: Detection of early induction of apoptosis was done using JC-1 dye by flow cytometry. Briefly, 1×10 5 cells/ml were treated with native nutlin-3a, Nut-NPs, Fol-Nut-NPs (1.5 µg/ml), curcumin, Cur-NPs, Fol-Cur-NPs (2 µg/ml) and Nutlin+Curcumin, Nut-Cur-NPs, Fol-Nut-Cur-NPs (1.5 nutlin+2 curcumin µg/ml) for 48 hrs. Cells treated with only medium was used as controls. After completion of incubation period, cells were treated with JC1 staining solution (1 µg/ml in warm DPBS) and incubated at 37°C for 20 min in a CO2 incubator. After incubation period, cells were examined by flow cytometer. Data represented as mean±s.e.m., (n = 3). Folate targeted dual drug loaded NPs showed higher loss of MMP in Y79 cells compared native drugs (single or in combination) and unconjugated single or dual drug loaded NPs.

    Techniques Used: Flow Cytometry, Cytometry, Incubation, Staining

    Related Articles

    Transduction:

    Article Title: Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells
    Article Snippet: .. After viral transduction, cells were maintained in a 37 ° C 5% CO2 incubator for 3 days in a defined media consisting of DMEM/F-12 (Invitrogen) supplemented with 20% Knockout Serum Replacement (Invitrogen), 1× Glutamax (Invitrogen), 1× Minimal Essential Media NonEssential Amino Acids Solution (Invitrogen), 1× Primocin (InvivoGen), 1× β-mercaptoethanol (Millipore, Billerica, MA), and 10 µg/ml basic fibroblast growth factor (bFGF) (Biopioneer, San Diego, CA). .. After 3 days, the cells were passaged with 0.05% trypsin EDTA (Gemini Bio-Products) onto a layer of mouse embryonic fibroblasts (GlobalStem, Rockville, MD) and maintained in culture up to 30 days until putative hiPSC colonies began to form.

    Stable Transfection:

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue
    Article Snippet: Transfection Procedure—Cell Cultures The murine fibrosarcoma L929A cells, stably transfected with the recombinant NF-κB-driven reporter gene construct p(IL6κB)3 50hu.IL6P-Luc+ and co-transfected with the reference reporter plasmid PGK-neogalactosidase, which constitutively expresses a neomycin resistance protein fused to a galactosidase reporter protein controlled by the housekeeping promoter phosphoglycerate kinase (PGK), were previously described [ , ]. .. L929sA and A549 (human lung epithelial) cells were grown at 37 °C, 95%–98% humidity and 5% CO2 incubator in Dulbecco Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA), supplied with 5% fetal calf serum, 5% Newborn calf serum (Greiner bio-one, Frickenhausen, Germany), 0.2% of P/S antibiotic (100 U/mL penicillin, 0.1 mg/mL streptomycin) (Gibco, Invitrogen) and 10% glutamine 2 mM.

    Cytometry:

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: Paragraph title: Cellular uptake study by flow cytometry ... Briefly, 6 well plates (Corning, NY, USA) were seeded with Y79 cells (folate receptor +ve) and A549 cells (folate receptor −ve) at 100,000 cells per well density and incubated with 1 ml of freshly prepared medium containing native curcumin, Cur-NPs, Fol-Cur-NPs (equivalent to 10 µg/ml concentrations of native curcumin) for 2 hrs at 37°C in CO2 incubator (Hera Cell, Thermo Scientific,Waltham, MA).

    Quantitative RT-PCR:

    Article Title: Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates
    Article Snippet: Paragraph title: Viremia determination by TCID50 and RT-qPCR ... NHP serum was serially diluted in growth media, added to VeroE6 cells and incubated for 1 h at 37 °C in a humidified 5% CO2 incubator, then overlaid with a preparation of 2× Eagle’s Basal medium (Gibco), 10% FBS (HyClone), and 1% SeaKem ME agarose (Lonza).

    Incubation:

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: .. Briefly, 6 well plates (Corning, NY, USA) were seeded with Y79 cells (folate receptor +ve) and A549 cells (folate receptor −ve) at 100,000 cells per well density and incubated with 1 ml of freshly prepared medium containing native curcumin, Cur-NPs, Fol-Cur-NPs (equivalent to 10 µg/ml concentrations of native curcumin) for 2 hrs at 37°C in CO2 incubator (Hera Cell, Thermo Scientific,Waltham, MA). .. At the end of the incubation period, the cells were collected and washed three times with cold DPBS to eliminate excess of native curcumin or NPs, which were not taken up by the cells.

    Article Title: Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates
    Article Snippet: .. NHP serum was serially diluted in growth media, added to VeroE6 cells and incubated for 1 h at 37 °C in a humidified 5% CO2 incubator, then overlaid with a preparation of 2× Eagle’s Basal medium (Gibco), 10% FBS (HyClone), and 1% SeaKem ME agarose (Lonza). .. Infected cells were incubated for 7 days, then stained with neutral red vital dye (Gibco).

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: .. Next days, 5 ml of media each containing 1.5 µg/ml concentrations of nutlin-3a (in solution or nanoformulations) or 2 µg/ml of curcumin (in solution or nanoformulations) or dual drugs (1.5 µg/ml nutlin-3a+2 µg/ml curcumin) in solution or nanoformulations were added to the flasks and the cells were incubated for 24 hrs in CO2 incubator at 37°C. .. After incubation time period, the cells were collected by centrifugation at 91 g (SIGMA 3K30, Munich, Germany) and washed twice with DPBS.

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: .. Subsequently, 5 ml of media each containing 1.5 µg/ml concentrations of nutlin-3a (in solution or nanoformulations) or 2 µg/ml of curcumin (in solution or nanoformulations) or dual drugs (1.5 µg/ml nutlin-3a+2 µg/ml curcumin) in solution or nanoformulations were added to the flasks and the cells were incubated for 2 days in CO2 incubator at 37°C. ..

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: .. The collected cells were then treated with JC-1 staining solution (1 µg/ml JC-1 in DPBS warm to 37°C) and incubated at 37°C for 20 min in a CO2 incubator. .. After incubation period, cells were washed twice with DPBS.

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: .. After overnight incubation, cells were treated with 5 ml of media each containing 1.5 µg/ml concentrations of nutlin-3a (in solution or nanoformulations) or 2 µg/ml of curcumin (in solution or nanoformulations) or dual drugs (1.5 µg/ml nutlin-3a+2 µg/ml curcumin) in solution or nanoformulations and then incubated for 2 days in CO2 incubator at 37°C. .. After completion of incubation period, cells were collected by centrifugation at 91 g (SIGMA 3K30, Munich, Germany) and washed twice with DPBS.

    Cell Culture:

    Article Title: MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway
    Article Snippet: .. Cells were cultured at 37 °C, in a 5% CO2 incubator, and the growth medium was DMEM supplemented with 10% FBS (GIBCO, USA), 10 mg/mL streptomycin, and 10 U/mL penicillin (Sigma, USA). ..

    Article Title: Overexpression of the hyperplasia suppressor gene inactivates airway fibroblasts obtained from a rat model of chronic obstructive pulmonary disease by inhibiting the Wnt signaling pathway
    Article Snippet: .. The tissue was pasted into the culture plate, and placed in a 5% CO2 incubator at 37°C for 4 h. After cell adherence, freshly prepared medium containing DMEM (Gibco; Thermo Fisher Scientific, Inc.) and 10% fetal bovine serum (FBS; cat. no. 04-007-1A; Biological Industries) was added to the culture plate, and the cells were further cultured in a 5% CO2 incubator at 37°C. ..

    Article Title: Myopathy-causing Actin Mutations Promote Defects in Serum Response Factor Signaling
    Article Snippet: .. C2C12 cells were cultured at 37 °C in humidified 5% CO2 incubator in a proliferation medium composed of Dulbecco's modified Eagle's medium (DMEM 41966, Gibco BRL, UK) supplemented with 10% fetal bovine serum (Sigma, UK). .. Transfections were made using Lipofectamine 2000 transfection reagent (Gibco BRL, UK) according to the manufacturer's protocol.

    Article Title: Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate
    Article Snippet: .. Osteoblast precursor cell line MC3T3-E1 derived from the C57BL/6 mouse calvarial (DS Pharma Biomedical Co., Ltd., Osaka, Japan) was cultured at 37˚C in a 5% CO2 incubator under humidified atmosphere in alpha minimum essential medium (α-MEM: GIBCO BRL, NY, USA) supplemented with 10% fetal bovine serum (FBS: Nichirei Biosciences Inc., Tokyo, Japan), penicillin G potassium (100 units/ml: Meiji Seika Pharma Co., Ltd., Tokyo, Japan) and streptomycin sulfate (100 μg/ml: Meiji Seika). .. Cells were detached with 0.25% trypsin (Sigma-Aldrich, St. Louis, MO, USA) and 0.025% EDTA (Wako Pure Chemical Industries, Ltd., Osaka, Japan).

    Modification:

    Article Title: MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway
    Article Snippet: Briefly, BMMSCs were isolated by flushing femur and tibia bones with Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, USA). .. Cells were cultured at 37 °C, in a 5% CO2 incubator, and the growth medium was DMEM supplemented with 10% FBS (GIBCO, USA), 10 mg/mL streptomycin, and 10 U/mL penicillin (Sigma, USA).

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue
    Article Snippet: .. L929sA and A549 (human lung epithelial) cells were grown at 37 °C, 95%–98% humidity and 5% CO2 incubator in Dulbecco Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA), supplied with 5% fetal calf serum, 5% Newborn calf serum (Greiner bio-one, Frickenhausen, Germany), 0.2% of P/S antibiotic (100 U/mL penicillin, 0.1 mg/mL streptomycin) (Gibco, Invitrogen) and 10% glutamine 2 mM. .. Treatment—Cell Lysis Twenty four hours before treatment with the compounds, L929 cells were seeded in 96-well plates such that they were confluent at the time of the experiment.

    Article Title: Myopathy-causing Actin Mutations Promote Defects in Serum Response Factor Signaling
    Article Snippet: .. C2C12 cells were cultured at 37 °C in humidified 5% CO2 incubator in a proliferation medium composed of Dulbecco's modified Eagle's medium (DMEM 41966, Gibco BRL, UK) supplemented with 10% fetal bovine serum (Sigma, UK). .. Transfections were made using Lipofectamine 2000 transfection reagent (Gibco BRL, UK) according to the manufacturer's protocol.

    Derivative Assay:

    Article Title: Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate
    Article Snippet: .. Osteoblast precursor cell line MC3T3-E1 derived from the C57BL/6 mouse calvarial (DS Pharma Biomedical Co., Ltd., Osaka, Japan) was cultured at 37˚C in a 5% CO2 incubator under humidified atmosphere in alpha minimum essential medium (α-MEM: GIBCO BRL, NY, USA) supplemented with 10% fetal bovine serum (FBS: Nichirei Biosciences Inc., Tokyo, Japan), penicillin G potassium (100 units/ml: Meiji Seika Pharma Co., Ltd., Tokyo, Japan) and streptomycin sulfate (100 μg/ml: Meiji Seika). .. Cells were detached with 0.25% trypsin (Sigma-Aldrich, St. Louis, MO, USA) and 0.025% EDTA (Wako Pure Chemical Industries, Ltd., Osaka, Japan).

    Transfection:

    Article Title: Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells
    Article Snippet: Fibroblasts were seeded at a confluency of 10,000 cells/cm2 prior to transfection. .. After viral transduction, cells were maintained in a 37 ° C 5% CO2 incubator for 3 days in a defined media consisting of DMEM/F-12 (Invitrogen) supplemented with 20% Knockout Serum Replacement (Invitrogen), 1× Glutamax (Invitrogen), 1× Minimal Essential Media NonEssential Amino Acids Solution (Invitrogen), 1× Primocin (InvivoGen), 1× β-mercaptoethanol (Millipore, Billerica, MA), and 10 µg/ml basic fibroblast growth factor (bFGF) (Biopioneer, San Diego, CA).

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue
    Article Snippet: Paragraph title: 3.2. Transfection Procedure—Cell Cultures ... L929sA and A549 (human lung epithelial) cells were grown at 37 °C, 95%–98% humidity and 5% CO2 incubator in Dulbecco Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA), supplied with 5% fetal calf serum, 5% Newborn calf serum (Greiner bio-one, Frickenhausen, Germany), 0.2% of P/S antibiotic (100 U/mL penicillin, 0.1 mg/mL streptomycin) (Gibco, Invitrogen) and 10% glutamine 2 mM.

    Article Title: Myopathy-causing Actin Mutations Promote Defects in Serum Response Factor Signaling
    Article Snippet: Paragraph title: Cell culture and transfection ... C2C12 cells were cultured at 37 °C in humidified 5% CO2 incubator in a proliferation medium composed of Dulbecco's modified Eagle's medium (DMEM 41966, Gibco BRL, UK) supplemented with 10% fetal bovine serum (Sigma, UK).

    Infection:

    Article Title: Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates
    Article Snippet: NHP serum was serially diluted in growth media, added to VeroE6 cells and incubated for 1 h at 37 °C in a humidified 5% CO2 incubator, then overlaid with a preparation of 2× Eagle’s Basal medium (Gibco), 10% FBS (HyClone), and 1% SeaKem ME agarose (Lonza). .. Infected cells were incubated for 7 days, then stained with neutral red vital dye (Gibco).

    Polymerase Chain Reaction:

    Article Title: Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates
    Article Snippet: NHP serum was serially diluted in growth media, added to VeroE6 cells and incubated for 1 h at 37 °C in a humidified 5% CO2 incubator, then overlaid with a preparation of 2× Eagle’s Basal medium (Gibco), 10% FBS (HyClone), and 1% SeaKem ME agarose (Lonza). .. For RT-qPCR, performed by the USAMRIID PCR Core Laboratory, serum or plasma was mixed 1:3 with Trizol LS (Thermo Fisher) and sample was extracted using the QIAamp Viral RNA Mini Kit in accordance with manufactures guidelines and USAMRIID Standard Operating Procedures.

    Recombinant:

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue
    Article Snippet: Transfection Procedure—Cell Cultures The murine fibrosarcoma L929A cells, stably transfected with the recombinant NF-κB-driven reporter gene construct p(IL6κB)3 50hu.IL6P-Luc+ and co-transfected with the reference reporter plasmid PGK-neogalactosidase, which constitutively expresses a neomycin resistance protein fused to a galactosidase reporter protein controlled by the housekeeping promoter phosphoglycerate kinase (PGK), were previously described [ , ]. .. L929sA and A549 (human lung epithelial) cells were grown at 37 °C, 95%–98% humidity and 5% CO2 incubator in Dulbecco Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA), supplied with 5% fetal calf serum, 5% Newborn calf serum (Greiner bio-one, Frickenhausen, Germany), 0.2% of P/S antibiotic (100 U/mL penicillin, 0.1 mg/mL streptomycin) (Gibco, Invitrogen) and 10% glutamine 2 mM.

    Fluorescence:

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: As curcumin has its intrinsic fluorescence property it serve as a fluorescence probe to efficiently investigate the uptake of drug loaded NPs. .. Briefly, 6 well plates (Corning, NY, USA) were seeded with Y79 cells (folate receptor +ve) and A549 cells (folate receptor −ve) at 100,000 cells per well density and incubated with 1 ml of freshly prepared medium containing native curcumin, Cur-NPs, Fol-Cur-NPs (equivalent to 10 µg/ml concentrations of native curcumin) for 2 hrs at 37°C in CO2 incubator (Hera Cell, Thermo Scientific,Waltham, MA).

    Isolation:

    Article Title: MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway
    Article Snippet: Paragraph title: Isolation and culture of BMMSCs ... Cells were cultured at 37 °C, in a 5% CO2 incubator, and the growth medium was DMEM supplemented with 10% FBS (GIBCO, USA), 10 mg/mL streptomycin, and 10 U/mL penicillin (Sigma, USA).

    Flow Cytometry:

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: Paragraph title: Cellular uptake study by flow cytometry ... Briefly, 6 well plates (Corning, NY, USA) were seeded with Y79 cells (folate receptor +ve) and A549 cells (folate receptor −ve) at 100,000 cells per well density and incubated with 1 ml of freshly prepared medium containing native curcumin, Cur-NPs, Fol-Cur-NPs (equivalent to 10 µg/ml concentrations of native curcumin) for 2 hrs at 37°C in CO2 incubator (Hera Cell, Thermo Scientific,Waltham, MA).

    Microscopy:

    Article Title: Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate
    Article Snippet: The surface of Ti powder, HA powder and plates was observed using a scanning electron microscope (SEM, JSM-6360LV: JEOL). .. Osteoblast precursor cell line MC3T3-E1 derived from the C57BL/6 mouse calvarial (DS Pharma Biomedical Co., Ltd., Osaka, Japan) was cultured at 37˚C in a 5% CO2 incubator under humidified atmosphere in alpha minimum essential medium (α-MEM: GIBCO BRL, NY, USA) supplemented with 10% fetal bovine serum (FBS: Nichirei Biosciences Inc., Tokyo, Japan), penicillin G potassium (100 units/ml: Meiji Seika Pharma Co., Ltd., Tokyo, Japan) and streptomycin sulfate (100 μg/ml: Meiji Seika).

    Construct:

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue
    Article Snippet: Transfection Procedure—Cell Cultures The murine fibrosarcoma L929A cells, stably transfected with the recombinant NF-κB-driven reporter gene construct p(IL6κB)3 50hu.IL6P-Luc+ and co-transfected with the reference reporter plasmid PGK-neogalactosidase, which constitutively expresses a neomycin resistance protein fused to a galactosidase reporter protein controlled by the housekeeping promoter phosphoglycerate kinase (PGK), were previously described [ , ]. .. L929sA and A549 (human lung epithelial) cells were grown at 37 °C, 95%–98% humidity and 5% CO2 incubator in Dulbecco Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA), supplied with 5% fetal calf serum, 5% Newborn calf serum (Greiner bio-one, Frickenhausen, Germany), 0.2% of P/S antibiotic (100 U/mL penicillin, 0.1 mg/mL streptomycin) (Gibco, Invitrogen) and 10% glutamine 2 mM.

    Staining:

    Article Title: Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates
    Article Snippet: NHP serum was serially diluted in growth media, added to VeroE6 cells and incubated for 1 h at 37 °C in a humidified 5% CO2 incubator, then overlaid with a preparation of 2× Eagle’s Basal medium (Gibco), 10% FBS (HyClone), and 1% SeaKem ME agarose (Lonza). .. Infected cells were incubated for 7 days, then stained with neutral red vital dye (Gibco).

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: .. The collected cells were then treated with JC-1 staining solution (1 µg/ml JC-1 in DPBS warm to 37°C) and incubated at 37°C for 20 min in a CO2 incubator. .. After incubation period, cells were washed twice with DPBS.

    Plasmid Preparation:

    Article Title: Inhibition of the NF-κB Signaling Pathway by a Novel Heterocyclic Curcumin Analogue
    Article Snippet: Transfection Procedure—Cell Cultures The murine fibrosarcoma L929A cells, stably transfected with the recombinant NF-κB-driven reporter gene construct p(IL6κB)3 50hu.IL6P-Luc+ and co-transfected with the reference reporter plasmid PGK-neogalactosidase, which constitutively expresses a neomycin resistance protein fused to a galactosidase reporter protein controlled by the housekeeping promoter phosphoglycerate kinase (PGK), were previously described [ , ]. .. L929sA and A549 (human lung epithelial) cells were grown at 37 °C, 95%–98% humidity and 5% CO2 incubator in Dulbecco Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen, Carlsbad, CA, USA), supplied with 5% fetal calf serum, 5% Newborn calf serum (Greiner bio-one, Frickenhausen, Germany), 0.2% of P/S antibiotic (100 U/mL penicillin, 0.1 mg/mL streptomycin) (Gibco, Invitrogen) and 10% glutamine 2 mM.

    In Vitro:

    Article Title: Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells
    Article Snippet: In vitro derivation and culture of human stem cell lines. .. After viral transduction, cells were maintained in a 37 ° C 5% CO2 incubator for 3 days in a defined media consisting of DMEM/F-12 (Invitrogen) supplemented with 20% Knockout Serum Replacement (Invitrogen), 1× Glutamax (Invitrogen), 1× Minimal Essential Media NonEssential Amino Acids Solution (Invitrogen), 1× Primocin (InvivoGen), 1× β-mercaptoethanol (Millipore, Billerica, MA), and 10 µg/ml basic fibroblast growth factor (bFGF) (Biopioneer, San Diego, CA).

    Knock-Out:

    Article Title: Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells
    Article Snippet: .. After viral transduction, cells were maintained in a 37 ° C 5% CO2 incubator for 3 days in a defined media consisting of DMEM/F-12 (Invitrogen) supplemented with 20% Knockout Serum Replacement (Invitrogen), 1× Glutamax (Invitrogen), 1× Minimal Essential Media NonEssential Amino Acids Solution (Invitrogen), 1× Primocin (InvivoGen), 1× β-mercaptoethanol (Millipore, Billerica, MA), and 10 µg/ml basic fibroblast growth factor (bFGF) (Biopioneer, San Diego, CA). .. After 3 days, the cells were passaged with 0.05% trypsin EDTA (Gemini Bio-Products) onto a layer of mouse embryonic fibroblasts (GlobalStem, Rockville, MD) and maintained in culture up to 30 days until putative hiPSC colonies began to form.

    Evaporation:

    Article Title: Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate
    Article Snippet: The morphology of proliferating cells on each plate was observed after washing with PBS (–), fixing with 2% glutaraldehyde and vacuum evaporation (200 Å) of deposited Au. .. Osteoblast precursor cell line MC3T3-E1 derived from the C57BL/6 mouse calvarial (DS Pharma Biomedical Co., Ltd., Osaka, Japan) was cultured at 37˚C in a 5% CO2 incubator under humidified atmosphere in alpha minimum essential medium (α-MEM: GIBCO BRL, NY, USA) supplemented with 10% fetal bovine serum (FBS: Nichirei Biosciences Inc., Tokyo, Japan), penicillin G potassium (100 units/ml: Meiji Seika Pharma Co., Ltd., Tokyo, Japan) and streptomycin sulfate (100 μg/ml: Meiji Seika).

    Concentration Assay:

    Article Title: Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates
    Article Snippet: NHP serum was serially diluted in growth media, added to VeroE6 cells and incubated for 1 h at 37 °C in a humidified 5% CO2 incubator, then overlaid with a preparation of 2× Eagle’s Basal medium (Gibco), 10% FBS (HyClone), and 1% SeaKem ME agarose (Lonza). .. Synthetic RNA representative of the target region of the SUDV or MARV was diluted in RNase free water was used to generate an eight-point standard curve for the quantitative determination of virus concentration using the Applied Biosystems 7500 Fast Dx System.

    Cell Counting:

    Article Title: Adhesion and Proliferation of Osteoblastic Cells on Hydroxyapatite-dispersed Ti-based Composite Plate
    Article Snippet: Osteoblast precursor cell line MC3T3-E1 derived from the C57BL/6 mouse calvarial (DS Pharma Biomedical Co., Ltd., Osaka, Japan) was cultured at 37˚C in a 5% CO2 incubator under humidified atmosphere in alpha minimum essential medium (α-MEM: GIBCO BRL, NY, USA) supplemented with 10% fetal bovine serum (FBS: Nichirei Biosciences Inc., Tokyo, Japan), penicillin G potassium (100 units/ml: Meiji Seika Pharma Co., Ltd., Tokyo, Japan) and streptomycin sulfate (100 μg/ml: Meiji Seika). .. WST-1 (Cell Counting Kit: Dojin Chem.

    FACS:

    Article Title: Folate Decorated Dual Drug Loaded Nanoparticle: Role of Curcumin in Enhancing Therapeutic Potential of Nutlin-3a by Reversing Multidrug Resistance
    Article Snippet: Briefly, 6 well plates (Corning, NY, USA) were seeded with Y79 cells (folate receptor +ve) and A549 cells (folate receptor −ve) at 100,000 cells per well density and incubated with 1 ml of freshly prepared medium containing native curcumin, Cur-NPs, Fol-Cur-NPs (equivalent to 10 µg/ml concentrations of native curcumin) for 2 hrs at 37°C in CO2 incubator (Hera Cell, Thermo Scientific,Waltham, MA). .. In all FACS analysis (FACScan flow cytometer, Becton Dickinson), cell debris and free particles were excluded by setting a gate on the plot of side-scattered light (SSC) vs forward-scattered light (FSC).

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  • 93
    Thermo Fisher cox 2 mouse monoclonal antibody
    Kaplan-Meier survival curves according to immunohistochemistry (IHC) scores for negligible and weak cyclooxygenase-2 <t>(COX-2)</t> expression (IHC scores 0–4) versus moderate and strong COX-2 expression (IHC scores 5–12) (p = 0.023; A), and the individual stratified categories (p = 0.002; B), analyzed using the log-rank test .
    Cox 2 Mouse Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cox 2 mouse monoclonal antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cox 2 mouse monoclonal antibody - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher cox2 primary antibody
    Extra-hematopoietic SK1 is necessary for <t>COX2</t> expression in the colon epithelium. COX2 expression levels were examined by IHC A–D ): untreated mice; E–H ) DSS treated mice. Scale bars = 20 µm. Regular text refers to the host genotype and the superscript to the bone marrow genotype.
    Cox2 Primary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cox2 primary antibody/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cox2 primary antibody - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher cox 2 primary human monoclonal antibody
    Non-neoplastic gastric mucosa negative for <t>COX-2</t> immunoreactivity.
    Cox 2 Primary Human Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cox 2 primary human monoclonal antibody/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cox 2 primary human monoclonal antibody - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Kaplan-Meier survival curves according to immunohistochemistry (IHC) scores for negligible and weak cyclooxygenase-2 (COX-2) expression (IHC scores 0–4) versus moderate and strong COX-2 expression (IHC scores 5–12) (p = 0.023; A), and the individual stratified categories (p = 0.002; B), analyzed using the log-rank test .

    Journal: Radiation Oncology (London, England)

    Article Title: Weak expression of cyclooxygenase-2 is associated with poorer outcome in endemic nasopharyngeal carcinoma: analysis of data from randomized trial between radiation alone versus concurrent chemo-radiation (SQNP-01)

    doi: 10.1186/1748-717X-4-23

    Figure Lengend Snippet: Kaplan-Meier survival curves according to immunohistochemistry (IHC) scores for negligible and weak cyclooxygenase-2 (COX-2) expression (IHC scores 0–4) versus moderate and strong COX-2 expression (IHC scores 5–12) (p = 0.023; A), and the individual stratified categories (p = 0.002; B), analyzed using the log-rank test .

    Article Snippet: The sections were incubated with COX-2 mouse monoclonal antibody (Neomarkers RM9121-S, Clone SP21, Thermo Fisher Scientific, Cheshire, UK) diluted 1:500 overnight at room temperature.

    Techniques: Immunohistochemistry, Expressing

    COX-2 Immunohistochemistry Patterns – typical examples . A: Negative staining for COX-2 ×200. B: Weak staining for COX-2 ×200. C: Moderate staining for COX-2 ×200. D: Strong staining for COX-2 ×200.

    Journal: Radiation Oncology (London, England)

    Article Title: Weak expression of cyclooxygenase-2 is associated with poorer outcome in endemic nasopharyngeal carcinoma: analysis of data from randomized trial between radiation alone versus concurrent chemo-radiation (SQNP-01)

    doi: 10.1186/1748-717X-4-23

    Figure Lengend Snippet: COX-2 Immunohistochemistry Patterns – typical examples . A: Negative staining for COX-2 ×200. B: Weak staining for COX-2 ×200. C: Moderate staining for COX-2 ×200. D: Strong staining for COX-2 ×200.

    Article Snippet: The sections were incubated with COX-2 mouse monoclonal antibody (Neomarkers RM9121-S, Clone SP21, Thermo Fisher Scientific, Cheshire, UK) diluted 1:500 overnight at room temperature.

    Techniques: Immunohistochemistry, Negative Staining, Staining

    Kaplan-Meier disease specific survival according to IHC scores for negligible and weak cyclooxygenase-2 (COX-2) expression (IHC scores 0–4) versus moderate and strong COX-2 expression (IHC scores 5–12) (p = 0.020; A), and the individual stratified categories (p = 0.006; B), analyzed using the log-rank test .

    Journal: Radiation Oncology (London, England)

    Article Title: Weak expression of cyclooxygenase-2 is associated with poorer outcome in endemic nasopharyngeal carcinoma: analysis of data from randomized trial between radiation alone versus concurrent chemo-radiation (SQNP-01)

    doi: 10.1186/1748-717X-4-23

    Figure Lengend Snippet: Kaplan-Meier disease specific survival according to IHC scores for negligible and weak cyclooxygenase-2 (COX-2) expression (IHC scores 0–4) versus moderate and strong COX-2 expression (IHC scores 5–12) (p = 0.020; A), and the individual stratified categories (p = 0.006; B), analyzed using the log-rank test .

    Article Snippet: The sections were incubated with COX-2 mouse monoclonal antibody (Neomarkers RM9121-S, Clone SP21, Thermo Fisher Scientific, Cheshire, UK) diluted 1:500 overnight at room temperature.

    Techniques: Immunohistochemistry, Expressing

    Extra-hematopoietic SK1 is necessary for COX2 expression in the colon epithelium. COX2 expression levels were examined by IHC A–D ): untreated mice; E–H ) DSS treated mice. Scale bars = 20 µm. Regular text refers to the host genotype and the superscript to the bone marrow genotype.

    Journal: PLoS ONE

    Article Title: Distinct Roles for Hematopoietic and Extra-Hematopoietic Sphingosine Kinase-1 in Inflammatory Bowel Disease

    doi: 10.1371/journal.pone.0113998

    Figure Lengend Snippet: Extra-hematopoietic SK1 is necessary for COX2 expression in the colon epithelium. COX2 expression levels were examined by IHC A–D ): untreated mice; E–H ) DSS treated mice. Scale bars = 20 µm. Regular text refers to the host genotype and the superscript to the bone marrow genotype.

    Article Snippet: Sections were then incubated for overnight at 4°C with the COX2 primary antibody (Thermo Scientific, Freemont, CA), phospho-STAT3 (Ser727, Cell Signaling, Danvers, MA), F4/80 (AbD Serotec, Raleigh, NC), or GR1-Ly6 (BD Pharmingen, San Jose, CA) followed washing with PBS and 30 min incubation with biotinylated secondary antibody and incubation with DAB reagent.

    Techniques: Expressing, Immunohistochemistry, Mouse Assay

    Non-neoplastic gastric mucosa negative for COX-2 immunoreactivity.

    Journal: Genetics and Molecular Biology

    Article Title: COX-2 gene expression and methylation profile in Sapajusapella as an experimental model for gastric adenocarcinoma

    doi: 10.1590/1678-4685-GMB-2016-0329

    Figure Lengend Snippet: Non-neoplastic gastric mucosa negative for COX-2 immunoreactivity.

    Article Snippet: The epitope retrieval was heat-induced followed by incubation with diluted (1:60) COX-2 primary human monoclonal antibody (Zymed/Thermo Fisher, COX2 Monoclonal Antibody COX 229, Catalog Number 35-8200).

    Techniques:

    Pre-neoplastic and neoplastic lesions positive for COX-2 immunoreactivity. (A) Chronic gastritis with plasmocytes; (B) Atrophic gastritis (atrophy); (C) Intestinal metaplasia; (D) Tumor

    Journal: Genetics and Molecular Biology

    Article Title: COX-2 gene expression and methylation profile in Sapajusapella as an experimental model for gastric adenocarcinoma

    doi: 10.1590/1678-4685-GMB-2016-0329

    Figure Lengend Snippet: Pre-neoplastic and neoplastic lesions positive for COX-2 immunoreactivity. (A) Chronic gastritis with plasmocytes; (B) Atrophic gastritis (atrophy); (C) Intestinal metaplasia; (D) Tumor

    Article Snippet: The epitope retrieval was heat-induced followed by incubation with diluted (1:60) COX-2 primary human monoclonal antibody (Zymed/Thermo Fisher, COX2 Monoclonal Antibody COX 229, Catalog Number 35-8200).

    Techniques: