Journal: The Journal of Neuroscience
Article Title: Ciliary Neurotrophic Factor (CNTF) Enhances Myelin Formation: A Novel Role for CNTF and CNTF-Related Molecules
Figure Lengend Snippet: Effect on myelination of different neurotrophic and growth factors. Myelinating cultures derived from 1900bp-MBP lacZ embryos were treated between 11 and 25 DIV; β-galactosidase activity was assayed at 25 DIV. A–C , Members of the neurotrophin ( A ) and GDNF ( B ) families and growth factors ( C ) were used at a concentration of 10 ng/ml, except for insulin, which was used at 100 μg/ml. D , Members of the CNTF family were tested at concentrations varying between 0.01 and 50 ng/ml. For each culture well, myelin formation was assessed by the quantification of the β-galactosidase level normalized to protein level (expressed as counts per minute per nanogram of protein). Results are expressed as the percentage of mean control values. A–C , Results are means ± SEM of three independent experiments with four to six cultures per experiment. D , Results are the means ± SEM of six cultures from one representative experiment.
Article Snippet: Mouse recombinant interleukin-6 (IL-6) and nerve growth factor (NGF), rat recombinant CNTF, human recombinant brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4, glial cell-derived neurotrophic factor (GDNF), neurturin (NTU), cardiotrophin-1 (CT-1), leukemia inhibitory factor (LIF), and fibroblast growth factor 2 (FGF-2) were obtained from Alomone Labs (Jerusalem, Israel).
Techniques: Derivative Assay, Activity Assay, Concentration Assay