cm2 econo column  (Bio-Rad)

 
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    Name:
    Econo Column Selection Pack A
    Description:
    Chromatography column pack includes 7 columns 1 each of 0 7 x 10 20 and 30 cm 1 5 x 30 and 50 cm 2 5 x 20 and 50 cm
    Catalog Number:
    7376601
    Price:
    None
    Category:
    Chromatography Systems Components and Accessories
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    Structured Review

    Bio-Rad cm2 econo column
    Econo Column Selection Pack A
    Chromatography column pack includes 7 columns 1 each of 0 7 x 10 20 and 30 cm 1 5 x 30 and 50 cm 2 5 x 20 and 50 cm
    https://www.bioz.com/result/cm2 econo column/product/Bio-Rad
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cm2 econo column - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Macaque Monoclonal Antibodies Targeting Novel Conserved Epitopes within Filovirus Glycoprotein
    Article Snippet: .. Upon overnight incubation at 4°C, beads were separated by centrifugation, resuspended in PBS plus 0.2% Tween 20, and packed into a Bio-Rad Econo-column. ..

    Affinity Chromatography:

    Article Title: Identification of a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells in Teleost Respiratory Surfaces
    Article Snippet: .. For this, rainbow trout IgM was purified from serum by affinity chromatography, using Econo-Column Chromatography columns (BIO-RAD) and CNBr-activated Sepharose 4b (GE Healthcare), coated with anti-trout IgM mAb (1.14), according to the manufacturer’s instructions. .. Thereafter, gill leukocytes were incubated with 0.1 µg/ml purified IgM for 1 h at 20°C.

    Chromatography:

    Article Title: Producing GST-Cbx7 Fusion Proteins from Escherichia coli
    Article Snippet: .. Materials and Reagents 50 ml conical tube Dialysis membrane (Spectra/Por® Molecularporous membrane tubing, standard RC tubing, MWCO: 3.5 kD) (Spectrum, catalog number: 132720) Bio-Rad Chromatography Column (2.5 × 10 cm Econo-Column) (Bio-Rad Laboratories, catalog number: 7311550) BL21 competent cells (made in the laboratory) pGEX-6P-1-GST (GE Healthcare) Ampicillin Isopropyl-1-thio-β-D-galactopyranoside (IPTG) (Omega Bio-tek, catalog number: AC121) Glutathione Sepharose 4B (GE Healthcare, catalog number: 17075601) Phosphate-buffered saline (PBS, pH 7.4) Pierce™ Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 23200) Coomassie Blue (Bio-Rad Laboratories, catalog number: 1610786) Tryptone Yeast extract Sodium chloride (NaCl) 1% Triton X-100 Phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich, catalog number: 93482) Protease inhibitor cocktail (Sigma-Aldrich, catalog number: P8340) L-glutathione reduced (Sigma-Aldrich, catalog number: G4251) LB medium (see Recipes) Wash buffer (see Recipes) Lysis buffer (see Recipes) Elution buffer (see Recipes) .. Equipment Culture flasks (2,000 ml) (NALGENE, U.S.A.) Shaker Centrifuges (Eppendorf, model: 5702 R) Centrifuge bottle Vibra-Cell™ sonicator (Sonics & Materials, model: VCX 130) with standard probe (1/4″; 6 mm), length 4.5″ (113 mm), Titanium alloy Ti-6Al-4V, Autoclavable (Sonics & Materials, catalog number: 630-0435) S-3200-2 GyroMixer (BioExpress, model: GeneMate GyroMixer, catalog number: S-3200-2)

    Article Title: Production and quality control assessment of a GLP-grade immunotoxin, D2C7-(scdsFv)-PE38KDEL, for a Phase I/II clinical trial
    Article Snippet: .. A 1 × 10 cm Econo-Column chromatography column (Bio-rad, Hercules, California) was packed with ActiClean Etox resin (Sterogene, Carlsbad, California). .. The column was equilibrated with 10 CVs of 1N NaOH and incubated over night at 4°C.

    Article Title: Identification of a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells in Teleost Respiratory Surfaces
    Article Snippet: .. For this, rainbow trout IgM was purified from serum by affinity chromatography, using Econo-Column Chromatography columns (BIO-RAD) and CNBr-activated Sepharose 4b (GE Healthcare), coated with anti-trout IgM mAb (1.14), according to the manufacturer’s instructions. .. Thereafter, gill leukocytes were incubated with 0.1 µg/ml purified IgM for 1 h at 20°C.

    Article Title: Kinetic and Structural Characterization of a cis-3-Chloroacrylic Acid Dehalogenase Homologue in Pseudomonas sp. UW4: A Potential Step Between Subgroups in the Tautomerase Superfamily
    Article Snippet: .. The Econo-Column chromatography columns (used for the DEAE-Sepharose columns) were obtained from Bio-Rad Laboratories, Inc. (Hercules, CA). .. The Amicon stirred cell concentrators and the ultrafiltration membranes (10000 Da, MW cutoff) were purchased from EMD Millipore Inc. (Billerica, MA).

    Article Title: Tet-Assisted Bisulfite Sequencing (TAB-seq)
    Article Snippet: .. Econo-Column chromatography columns (Bio-Rad, 2.5 × 20 cm). .. 3× Flag peptide (Sigma-Aldrich).

    Protease Inhibitor:

    Article Title: Producing GST-Cbx7 Fusion Proteins from Escherichia coli
    Article Snippet: .. Materials and Reagents 50 ml conical tube Dialysis membrane (Spectra/Por® Molecularporous membrane tubing, standard RC tubing, MWCO: 3.5 kD) (Spectrum, catalog number: 132720) Bio-Rad Chromatography Column (2.5 × 10 cm Econo-Column) (Bio-Rad Laboratories, catalog number: 7311550) BL21 competent cells (made in the laboratory) pGEX-6P-1-GST (GE Healthcare) Ampicillin Isopropyl-1-thio-β-D-galactopyranoside (IPTG) (Omega Bio-tek, catalog number: AC121) Glutathione Sepharose 4B (GE Healthcare, catalog number: 17075601) Phosphate-buffered saline (PBS, pH 7.4) Pierce™ Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 23200) Coomassie Blue (Bio-Rad Laboratories, catalog number: 1610786) Tryptone Yeast extract Sodium chloride (NaCl) 1% Triton X-100 Phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich, catalog number: 93482) Protease inhibitor cocktail (Sigma-Aldrich, catalog number: P8340) L-glutathione reduced (Sigma-Aldrich, catalog number: G4251) LB medium (see Recipes) Wash buffer (see Recipes) Lysis buffer (see Recipes) Elution buffer (see Recipes) .. Equipment Culture flasks (2,000 ml) (NALGENE, U.S.A.) Shaker Centrifuges (Eppendorf, model: 5702 R) Centrifuge bottle Vibra-Cell™ sonicator (Sonics & Materials, model: VCX 130) with standard probe (1/4″; 6 mm), length 4.5″ (113 mm), Titanium alloy Ti-6Al-4V, Autoclavable (Sonics & Materials, catalog number: 630-0435) S-3200-2 GyroMixer (BioExpress, model: GeneMate GyroMixer, catalog number: S-3200-2)

    Purification:

    Article Title: Identification of a Potential Common Ancestor for Mammalian Cross-Presenting Dendritic Cells in Teleost Respiratory Surfaces
    Article Snippet: .. For this, rainbow trout IgM was purified from serum by affinity chromatography, using Econo-Column Chromatography columns (BIO-RAD) and CNBr-activated Sepharose 4b (GE Healthcare), coated with anti-trout IgM mAb (1.14), according to the manufacturer’s instructions. .. Thereafter, gill leukocytes were incubated with 0.1 µg/ml purified IgM for 1 h at 20°C.

    Incubation:

    Article Title: Macaque Monoclonal Antibodies Targeting Novel Conserved Epitopes within Filovirus Glycoprotein
    Article Snippet: .. Upon overnight incubation at 4°C, beads were separated by centrifugation, resuspended in PBS plus 0.2% Tween 20, and packed into a Bio-Rad Econo-column. ..

    Lysis:

    Article Title: Producing GST-Cbx7 Fusion Proteins from Escherichia coli
    Article Snippet: .. Materials and Reagents 50 ml conical tube Dialysis membrane (Spectra/Por® Molecularporous membrane tubing, standard RC tubing, MWCO: 3.5 kD) (Spectrum, catalog number: 132720) Bio-Rad Chromatography Column (2.5 × 10 cm Econo-Column) (Bio-Rad Laboratories, catalog number: 7311550) BL21 competent cells (made in the laboratory) pGEX-6P-1-GST (GE Healthcare) Ampicillin Isopropyl-1-thio-β-D-galactopyranoside (IPTG) (Omega Bio-tek, catalog number: AC121) Glutathione Sepharose 4B (GE Healthcare, catalog number: 17075601) Phosphate-buffered saline (PBS, pH 7.4) Pierce™ Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific, Thermo Scientific™, catalog number: 23200) Coomassie Blue (Bio-Rad Laboratories, catalog number: 1610786) Tryptone Yeast extract Sodium chloride (NaCl) 1% Triton X-100 Phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich, catalog number: 93482) Protease inhibitor cocktail (Sigma-Aldrich, catalog number: P8340) L-glutathione reduced (Sigma-Aldrich, catalog number: G4251) LB medium (see Recipes) Wash buffer (see Recipes) Lysis buffer (see Recipes) Elution buffer (see Recipes) .. Equipment Culture flasks (2,000 ml) (NALGENE, U.S.A.) Shaker Centrifuges (Eppendorf, model: 5702 R) Centrifuge bottle Vibra-Cell™ sonicator (Sonics & Materials, model: VCX 130) with standard probe (1/4″; 6 mm), length 4.5″ (113 mm), Titanium alloy Ti-6Al-4V, Autoclavable (Sonics & Materials, catalog number: 630-0435) S-3200-2 GyroMixer (BioExpress, model: GeneMate GyroMixer, catalog number: S-3200-2)

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  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
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  • Contact
  • Bioz Stars
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  • 99
    Bio-Rad cm2 econo column
    Cm2 Econo Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cm2 econo column/product/Bio-Rad
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cm2 econo column - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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