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Philips Healthcare cm10 electron microscope
Electron microscopy analysis of HEK-apoE. Pooled fractions 35-45 of HEK-apoE isolated by SEC were concentrated and an aliquot placed on a carbon-coated electron microscopy grid and negatively stained with 2% phosphotungstic acid. Using a Philips <t>CM10</t> electron microscope, the diameters of 100 intact particles from an enlarged photomicrograph were measured by using a micrometer lens. Representative particles are shown below, with a mean diameter of 12nm ± 1.87 standard deviations. Results shown are for HEK-apoE3. Comparable results were observed using HEK-apoE4 (data not shown).
Cm10 Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Self-Assembly of HEK Cell-Secreted ApoE Particles Resemble ApoE-Enrichment of Lipoproteins as a Ligand for the LDL Receptor-Related Protein

Journal: Biochemistry

doi: 10.1021/bi051765s

Electron microscopy analysis of HEK-apoE. Pooled fractions 35-45 of HEK-apoE isolated by SEC were concentrated and an aliquot placed on a carbon-coated electron microscopy grid and negatively stained with 2% phosphotungstic acid. Using a Philips CM10 electron microscope, the diameters of 100 intact particles from an enlarged photomicrograph were measured by using a micrometer lens. Representative particles are shown below, with a mean diameter of 12nm ± 1.87 standard deviations. Results shown are for HEK-apoE3. Comparable results were observed using HEK-apoE4 (data not shown).
Figure Legend Snippet: Electron microscopy analysis of HEK-apoE. Pooled fractions 35-45 of HEK-apoE isolated by SEC were concentrated and an aliquot placed on a carbon-coated electron microscopy grid and negatively stained with 2% phosphotungstic acid. Using a Philips CM10 electron microscope, the diameters of 100 intact particles from an enlarged photomicrograph were measured by using a micrometer lens. Representative particles are shown below, with a mean diameter of 12nm ± 1.87 standard deviations. Results shown are for HEK-apoE3. Comparable results were observed using HEK-apoE4 (data not shown).

Techniques Used: Electron Microscopy, Isolation, Size-exclusion Chromatography, Staining, Microscopy

Related Articles

Electron Microscopy:

Article Title: Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles
Article Snippet: .. The labeled filters were then prepared for plastic embedding by Karnovsky fixation (4% paraformaldehyde + 2.5% glutaraldehyde in 0.1 M cacodylate buffer pH7.2), followed by staining with 1(w/v)% osmiumtetroxide for 1 hour, dehydration by isopropanol series, toluene and embedded in embed 812 (Electron Microscopy Sciences), sectioned and viewed with a Philips CM10 Electron Microscope. .. Hybrid vesicles are formed after mechanical membrane disruption To monitor possible effects on fusion by sheer force-based disruption methods, MelJuSo (MJS) cells stably transfected with Tap1-GFP were stained with anti-MHC-II antibody L243-Alexa 647nm at low temperature to prevent endocytosis thus effectively labelling only the plasma membrane pool of MHC-II.

Article Title: Self-Assembly of HEK Cell-Secreted ApoE Particles Resemble ApoE-Enrichment of Lipoproteins as a Ligand for the LDL Receptor-Related Protein
Article Snippet: .. Pooled fractions 35-45 of HEK-apoE isolated by SEC were examined by using the Philips CM10 electron microscope at the Electron Microscopy Core Facility of the University of Chicago as described previously ( , ). ..

Isolation:

Article Title: Self-Assembly of HEK Cell-Secreted ApoE Particles Resemble ApoE-Enrichment of Lipoproteins as a Ligand for the LDL Receptor-Related Protein
Article Snippet: .. Pooled fractions 35-45 of HEK-apoE isolated by SEC were examined by using the Philips CM10 electron microscope at the Electron Microscopy Core Facility of the University of Chicago as described previously ( , ). ..

Size-exclusion Chromatography:

Article Title: Self-Assembly of HEK Cell-Secreted ApoE Particles Resemble ApoE-Enrichment of Lipoproteins as a Ligand for the LDL Receptor-Related Protein
Article Snippet: .. Pooled fractions 35-45 of HEK-apoE isolated by SEC were examined by using the Philips CM10 electron microscope at the Electron Microscopy Core Facility of the University of Chicago as described previously ( , ). ..

Microscopy:

Article Title: Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells
Article Snippet: .. Images were captured by a Philips CM10 electron microscope using 100 kV voltage. .. TEM micrographs were taken by a Megaview G2 digital camera (ITEM, Olympus).

Article Title: Ongoing Viral Replication Is Required for Gammaherpesvirus 68-Induced Vascular Damage
Article Snippet: .. Specimens were evaluated with a Philips CM10 electron microscope. .. Cidofovir (Vistide; Gilead Sciences, Foster City, Calif.) was diluted in low-endotoxin PBS to 6.25 mg/ml and filter sterilized.

Article Title: Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles
Article Snippet: .. The labeled filters were then prepared for plastic embedding by Karnovsky fixation (4% paraformaldehyde + 2.5% glutaraldehyde in 0.1 M cacodylate buffer pH7.2), followed by staining with 1(w/v)% osmiumtetroxide for 1 hour, dehydration by isopropanol series, toluene and embedded in embed 812 (Electron Microscopy Sciences), sectioned and viewed with a Philips CM10 Electron Microscope. .. Hybrid vesicles are formed after mechanical membrane disruption To monitor possible effects on fusion by sheer force-based disruption methods, MelJuSo (MJS) cells stably transfected with Tap1-GFP were stained with anti-MHC-II antibody L243-Alexa 647nm at low temperature to prevent endocytosis thus effectively labelling only the plasma membrane pool of MHC-II.

Article Title: An integrin-ILK-microtubule network orients cell polarity and lumen formation in glandular epithelium
Article Snippet: .. Images were captured on a Philips CM10 electron microscope at an accelerating voltage of 80kV, with a Deben camera. .. Cell fractionation Acini were isolated using Matrisperse (BD Biosciences), lysed in hypotonic lysis buffer (10mM Tris pH7.5, 1.5mM magnesium chloride, 10mM sodium chloride 10μg/ml leupeptin, 10μg/ml aprotinin, 1mM sodium fluoride, 1mM sodium orthovanadate, 1mM PMSF) and cells were fractionated into cytosolic and membrane fractions .

Article Title: The Epithelial Cell Adhesion Molecule EpCAM Is Required for Epithelial Morphogenesis and Integrity during Zebrafish Epiboly and Skin Development
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and viewed in Philips CM10 electron microscope. .. Supporting Information epcam mutants form skin cell aggregates. (A) Confocal image (merged Z-stack) of basal keratinocytes of wild type control (WT) and maternal/zygotic epcam mutant (MZ−/−) embryos at 2 days post fertilization (dpf), after anti-p63 immunostaining of basal keratinocytes.

Article Title: Self-Assembly of HEK Cell-Secreted ApoE Particles Resemble ApoE-Enrichment of Lipoproteins as a Ligand for the LDL Receptor-Related Protein
Article Snippet: .. Pooled fractions 35-45 of HEK-apoE isolated by SEC were examined by using the Philips CM10 electron microscope at the Electron Microscopy Core Facility of the University of Chicago as described previously ( , ). ..

Article Title: Transcript and metabolite signature of maize source leaves suggests a link between transitory starch to sucrose balance and the autonomous floral transition
Article Snippet: .. Sectioned material was examined with a Philips CM10 electron microscope. .. Carboxyfluorescein diacetate (CFDA) studies The blade of L5 of plants at the V7 stage was cut under water and submerged in a 50 µg ml–1 (5-,6-) carboxyfluorescein diacetate (CFDA; Invitrogen) solution to evaluate phloem transport of the dye as previously described ( ).

Article Title: A non-enzymatic function of 17?-hydroxysteroid dehydrogenase type 10 is required for mitochondrial integrity and cell survival
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate (Serva Electrophoresis) (Venable & Coggeshall, ) and viewed in a Philips CM10 electron microscope at 60 kV using a 30 µm objective aperture. ..

Labeling:

Article Title: Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles
Article Snippet: .. The labeled filters were then prepared for plastic embedding by Karnovsky fixation (4% paraformaldehyde + 2.5% glutaraldehyde in 0.1 M cacodylate buffer pH7.2), followed by staining with 1(w/v)% osmiumtetroxide for 1 hour, dehydration by isopropanol series, toluene and embedded in embed 812 (Electron Microscopy Sciences), sectioned and viewed with a Philips CM10 Electron Microscope. .. Hybrid vesicles are formed after mechanical membrane disruption To monitor possible effects on fusion by sheer force-based disruption methods, MelJuSo (MJS) cells stably transfected with Tap1-GFP were stained with anti-MHC-II antibody L243-Alexa 647nm at low temperature to prevent endocytosis thus effectively labelling only the plasma membrane pool of MHC-II.

Staining:

Article Title: Mechanical Forces Used for Cell Fractionation Can Create Hybrid Membrane Vesicles
Article Snippet: .. The labeled filters were then prepared for plastic embedding by Karnovsky fixation (4% paraformaldehyde + 2.5% glutaraldehyde in 0.1 M cacodylate buffer pH7.2), followed by staining with 1(w/v)% osmiumtetroxide for 1 hour, dehydration by isopropanol series, toluene and embedded in embed 812 (Electron Microscopy Sciences), sectioned and viewed with a Philips CM10 Electron Microscope. .. Hybrid vesicles are formed after mechanical membrane disruption To monitor possible effects on fusion by sheer force-based disruption methods, MelJuSo (MJS) cells stably transfected with Tap1-GFP were stained with anti-MHC-II antibody L243-Alexa 647nm at low temperature to prevent endocytosis thus effectively labelling only the plasma membrane pool of MHC-II.

Article Title: The Epithelial Cell Adhesion Molecule EpCAM Is Required for Epithelial Morphogenesis and Integrity during Zebrafish Epiboly and Skin Development
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate and viewed in Philips CM10 electron microscope. .. Supporting Information epcam mutants form skin cell aggregates. (A) Confocal image (merged Z-stack) of basal keratinocytes of wild type control (WT) and maternal/zygotic epcam mutant (MZ−/−) embryos at 2 days post fertilization (dpf), after anti-p63 immunostaining of basal keratinocytes.

Article Title: A non-enzymatic function of 17?-hydroxysteroid dehydrogenase type 10 is required for mitochondrial integrity and cell survival
Article Snippet: .. Ultrathin sections were stained with uranyl acetate and lead citrate (Serva Electrophoresis) (Venable & Coggeshall, ) and viewed in a Philips CM10 electron microscope at 60 kV using a 30 µm objective aperture. ..

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    Philips Healthcare cm10 electron microscope
    Electron microscopy analysis of HEK-apoE. Pooled fractions 35-45 of HEK-apoE isolated by SEC were concentrated and an aliquot placed on a carbon-coated electron microscopy grid and negatively stained with 2% phosphotungstic acid. Using a Philips <t>CM10</t> electron microscope, the diameters of 100 intact particles from an enlarged photomicrograph were measured by using a micrometer lens. Representative particles are shown below, with a mean diameter of 12nm ± 1.87 standard deviations. Results shown are for HEK-apoE3. Comparable results were observed using HEK-apoE4 (data not shown).
    Cm10 Electron Microscope, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cm10 electron microscope/product/Philips Healthcare
    Average 94 stars, based on 860 article reviews
    Price from $9.99 to $1999.99
    cm10 electron microscope - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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    Electron microscopy analysis of HEK-apoE. Pooled fractions 35-45 of HEK-apoE isolated by SEC were concentrated and an aliquot placed on a carbon-coated electron microscopy grid and negatively stained with 2% phosphotungstic acid. Using a Philips CM10 electron microscope, the diameters of 100 intact particles from an enlarged photomicrograph were measured by using a micrometer lens. Representative particles are shown below, with a mean diameter of 12nm ± 1.87 standard deviations. Results shown are for HEK-apoE3. Comparable results were observed using HEK-apoE4 (data not shown).

    Journal: Biochemistry

    Article Title: Self-Assembly of HEK Cell-Secreted ApoE Particles Resemble ApoE-Enrichment of Lipoproteins as a Ligand for the LDL Receptor-Related Protein

    doi: 10.1021/bi051765s

    Figure Lengend Snippet: Electron microscopy analysis of HEK-apoE. Pooled fractions 35-45 of HEK-apoE isolated by SEC were concentrated and an aliquot placed on a carbon-coated electron microscopy grid and negatively stained with 2% phosphotungstic acid. Using a Philips CM10 electron microscope, the diameters of 100 intact particles from an enlarged photomicrograph were measured by using a micrometer lens. Representative particles are shown below, with a mean diameter of 12nm ± 1.87 standard deviations. Results shown are for HEK-apoE3. Comparable results were observed using HEK-apoE4 (data not shown).

    Article Snippet: Pooled fractions 35-45 of HEK-apoE isolated by SEC were examined by using the Philips CM10 electron microscope at the Electron Microscopy Core Facility of the University of Chicago as described previously ( , ).

    Techniques: Electron Microscopy, Isolation, Size-exclusion Chromatography, Staining, Microscopy

    Electron microscopy images of chloroplasts from wild type (WT) and the RNAi-W1-7 plants (W1-7) . Leaf segments (2 × 2 mm) from 10 days old primary foliage leaves were taken at a position of 2 cm below the leaf tip. Some of the DNA containing regions are indicated by arrows. Transmission electron microscopy was performed with a Philips CM10 transmission electron microscope. Bars represent 500 nm.

    Journal: Frontiers in Plant Science

    Article Title: WHIRLY1 is a major organizer of chloroplast nucleoids

    doi: 10.3389/fpls.2014.00432

    Figure Lengend Snippet: Electron microscopy images of chloroplasts from wild type (WT) and the RNAi-W1-7 plants (W1-7) . Leaf segments (2 × 2 mm) from 10 days old primary foliage leaves were taken at a position of 2 cm below the leaf tip. Some of the DNA containing regions are indicated by arrows. Transmission electron microscopy was performed with a Philips CM10 transmission electron microscope. Bars represent 500 nm.

    Article Snippet: The sections were stained with saturated uranyl acetate in water and lead citrate (Reynolds, ) and observed using a Philips CM10 transmission electron microscope (FEI, Eindhoven, The Netherlands).

    Techniques: Electron Microscopy, Transmission Assay, Microscopy

    TEM of rmMBP-actin bundles obtained at a 1:1.5 molar ratio of actin to rmMBP and negatively stained with uranyl acetate. The image was captured on a Philips CM10 transmission electron microscope, using a fourfold-diluted fraction of a preparation typical of that used for NMR spectroscopy.

    Journal: Biophysical Journal

    Article Title: Induced Secondary Structure and Polymorphism in an Intrinsically Disordered Structural Linker of the CNS: Solid-State NMR and FTIR Spectroscopy of Myelin Basic Protein Bound to Actin

    doi: 10.1016/j.bpj.2008.10.003

    Figure Lengend Snippet: TEM of rmMBP-actin bundles obtained at a 1:1.5 molar ratio of actin to rmMBP and negatively stained with uranyl acetate. The image was captured on a Philips CM10 transmission electron microscope, using a fourfold-diluted fraction of a preparation typical of that used for NMR spectroscopy.

    Article Snippet: The samples were negatively stained with uranyl acetate, dried, and examined on a Philips CM10 transmission electron microscope.

    Techniques: Transmission Electron Microscopy, Staining, Transmission Assay, Microscopy, Nuclear Magnetic Resonance, Spectroscopy

    TEM analysis. Representative negatively stained transmission electron microscopy images of the four FSs (F1–F4) taken at 92,000× magnification (scale bar: 200 nm) with a CM10 Philips transmission electron microscope equipped with Megaview 3 camera and Olympus SIS iTEM software for digital image acquisition. ( A ) F1; ( B ) F2; ( C ) F3; and, ( D ) F4. All of the FSs show long unbranched banded fibrils of uniform size and periodicity band pattern. F1, F2, and F4 show small clots and filaments (white arrows) of putative mucopolysaccharides.

    Journal: Marine Drugs

    Article Title: Production, Characterization and Biocompatibility Evaluation of Collagen Membranes Derived from Marine Sponge Chondrosia reniformis Nardo, 1847

    doi: 10.3390/md16040111

    Figure Lengend Snippet: TEM analysis. Representative negatively stained transmission electron microscopy images of the four FSs (F1–F4) taken at 92,000× magnification (scale bar: 200 nm) with a CM10 Philips transmission electron microscope equipped with Megaview 3 camera and Olympus SIS iTEM software for digital image acquisition. ( A ) F1; ( B ) F2; ( C ) F3; and, ( D ) F4. All of the FSs show long unbranched banded fibrils of uniform size and periodicity band pattern. F1, F2, and F4 show small clots and filaments (white arrows) of putative mucopolysaccharides.

    Article Snippet: FS samples were imaged with a CM10 Philips transmission electron microscope equipped with Megaview 3 camera and Olympus SIS iTEM software for digital image acquisition.

    Techniques: Transmission Electron Microscopy, Staining, Transmission Assay, Electron Microscopy, Microscopy, Software