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Eyes harvested from Thbs1 -deficient mice treated with vehicle or 50µg clusterin (pCLU or rCLU) or 0.1 % dexamethasone (Dex.) (positive control) for up to 3 weeks (5 days/week) were harvested, and parafin-embedded sections were immunostained for ADAM17 as described in the methods. ( A ) Representative immunofluorescence images of ADAM17 expression (red) in the corneal Epithelium (E) from the indicated treatment groups are shown. ( B ) Quantitative analysis of ADAM17 staining within the corneal epithelium presented as the ratio of ADAM17 (red) to DAPI (blue) staining. Data presented as mean ± SEM (**p < 0.01).

Journal: bioRxiv

Article Title: Clusterin reverses epitheliopathy, reduces inflammation, and restores goblet cells and corneal nerves in a mouse model of autoimmune dry eye

doi: 10.1101/2025.06.05.658110

Figure Lengend Snippet: Eyes harvested from Thbs1 -deficient mice treated with vehicle or 50µg clusterin (pCLU or rCLU) or 0.1 % dexamethasone (Dex.) (positive control) for up to 3 weeks (5 days/week) were harvested, and parafin-embedded sections were immunostained for ADAM17 as described in the methods. ( A ) Representative immunofluorescence images of ADAM17 expression (red) in the corneal Epithelium (E) from the indicated treatment groups are shown. ( B ) Quantitative analysis of ADAM17 staining within the corneal epithelium presented as the ratio of ADAM17 (red) to DAPI (blue) staining. Data presented as mean ± SEM (**p < 0.01).

Article Snippet: M.E.F. is named as a co-inventor on U.S. patent number 9241974 entitled "Clusterin Pharmaceuticals and Treatment Methods Using the Same" granted to the University of Southern California and optioned for exclusive license to Proteris Biotech.

Techniques: Positive Control, Immunofluorescence, Expressing, Staining