Clozapine N Oxide, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Prenatal Exposure to Ketamine Leads to Anxiety-Like Behaviors and Dysfunction in Bed Nucleus of Stria Terminalis"
Article Title: Prenatal Exposure to Ketamine Leads to Anxiety-Like Behaviors and Dysfunction in Bed Nucleus of Stria Terminalis
Journal: International Journal of Neuropsychopharmacology
Figure Legend Snippet: The flow chart of the experimental procedure. CNO, clozapine N-oxide; DREADDs, designer receptors exclusively activated by designer drugs; E, gestation day; EPM, elevated plus maze; FCS, fear conditioning test; IHC, immunohistochemistry; OFT, open field test; P, postnatal day.
Techniques Used: Immunohistochemistry
2) Product Images from "A critical period of neuronal activity results in aberrant neurogenesis rewiring hippocampal circuitry in a mouse model of epilepsy"
Article Title: A critical period of neuronal activity results in aberrant neurogenesis rewiring hippocampal circuitry in a mouse model of epilepsy
Journal: Nature Communications
Figure Legend Snippet: Activation of immature adult-born granule cells promote aberrant neurogenesis and seizures. a Retrovirus was injected into the hippocampus to express the activating chemogenetic receptor (CAG-hM3Dq-GFP) to infect the neural stem cells. Clozapine-N-oxide (1 mg/kg) was administered daily to activate maturing granule cells for different periods of time. At 8 weeks post-infection, mice were implanted for video-EEG to monitor for seizures and then perfused for histology. b Representative images of an ectopic granule cell located in the hilus. Arrow indicates cell body. Scale bar represents 50 µm. c Quantified migration distance of GFP + cells activated during the first week of maturation (0–1w; green; n = 78, 91, 90, and 69 cells per group, respectively, from five mice per group). d Migration distance of GFP + cells activated from the second week (1–2w; orange; n = 81, 84, 79, 69 cells per group, respectively, from five mice per group). Data points represent individual cells. Zero represents SGZ (white), less than zero represents hilus (red), and above ten represents GCL (blue). e Quantification of the total number of GFP + cells in the 0–1w group. Gray bars indicate vehicle treatment (Veh). Green bars indicate CNO treatment. n = 5 mice per group. f Quantification of the GFP + EGCs in 0–1w group. n = 5 mice per group. g Quantification of total number of GFP + cells in 1–2w group. Gray bars indicate Veh and orange indicate CNO treatment. n = 5 mice per group. h Quantification of GFP + EGCs in 1–2w group. n = 5 mice per group. * p
Techniques Used: Activation Assay, Injection, Infection, Mouse Assay, Migration
Figure Legend Snippet: Silencing immature adult-born granule cells in pilocarpine model prevents abnormal maturation. a Schematic of epilepsy model. Retrovirus hM4Di-GFP (green), or the control CAG-GFP (gray), was injected for two consecutive days then given pilocarpine (pilo) to induce the status epilepticus model for epilepsy. Two weeks following pilo, clozapine-N-oxide (CNO) was administered twice daily to silence immature abGCs. Mice were implanted for video-EEG recording and monitored from 8 to 10 weeks post-pilo for SRS and perfused for histology following EEG monitoring. b Representative images of the dentate gyrus from epileptic mice. Scale bar equal 50 µm. c Migration distance of GFP + cells from the SGZ in sham (left; n = 207, 192, 122, and 102 cells per group, respectively, from 15 mice per group) and pilo (right; n = 187, 147, 115, and 126 cells per group, respectively, from 8, 8, 6, and 14 mice per group). Each dot represents an individual cell. d Quantification of total number of GFP cells from pilo group. e Quantification for the total number of GFP + cells located in the hilus. For ( d ) and ( e ), n = 8, 8, 6, and 14 mice per group, respectively. Error bars represent SEM, * p
Techniques Used: Injection, Mouse Assay, Migration