Review



clones myosin x shrna m lentiviral particles  (Santa Cruz Biotechnology)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology clones myosin x shrna m lentiviral particles
    Western blot analyses of Nef (a) and <t>Myo10</t> (c) expression levels in Raw 264.7 cells (1), Raw 264.7 treated with CdCl2 (2), N5 cells (3) and N5 treated with CdCl2 (4). GAPDH and Calnexin were used as loading controls for Nef and Myo10 respectively. Treatment of N5 cells with CdCl2 increases both Nef and Myo10 expression (lanes 3 vs 4). Blots are representative of 4 independent experiments. Densitometry representation of Nef expression (b) or Myo10 expression (d) from 4 independent experiments. Graphs show the means (± s.e.m), with a P value <0.01 (**) or < 0.02 (*). Representative fluorescence images of Raw 264.7 cells treated with CdCl2 (e) or N5 treated with CdCl2 (f). The plasma membrane was labeled with WGA-Rhodamine. Z-stacks at the level of the substratum identifying filopodia or above the substratum identifying TNTs are shown. Treatment of N5 cells with CdCl2 increases both the length of filopodia and the amount of TNTs (white arrows) observed. Quantification of the number of TNTs in Raw 264.7 cells (± CdCl2) (g) or N5 cells (± CdCl2) (h). We observed a decrease in the number of cells with TNTs in Raw 264.7 cells treated with CdCl2 compared to the untreated control cells (g) and a 40% increase in the number of cells connected by TNTs in N5 treated with CdCl2 (h). Data are the average of 4 independent experiments and the graphs show the means (± s.e.m), with a P value <0.01 (**)
    Clones Myosin X Shrna M Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clones myosin x shrna m lentiviral particles/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    clones myosin x shrna m lentiviral particles - by Bioz Stars, 2025-03
    90/100 stars

    Images

    1) Product Images from "Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes"

    Article Title: Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes

    Journal: Journal of Cell Communication and Signaling

    doi: 10.1007/s12079-018-0493-z

    Western blot analyses of Nef (a) and Myo10 (c) expression levels in Raw 264.7 cells (1), Raw 264.7 treated with CdCl2 (2), N5 cells (3) and N5 treated with CdCl2 (4). GAPDH and Calnexin were used as loading controls for Nef and Myo10 respectively. Treatment of N5 cells with CdCl2 increases both Nef and Myo10 expression (lanes 3 vs 4). Blots are representative of 4 independent experiments. Densitometry representation of Nef expression (b) or Myo10 expression (d) from 4 independent experiments. Graphs show the means (± s.e.m), with a P value <0.01 (**) or < 0.02 (*). Representative fluorescence images of Raw 264.7 cells treated with CdCl2 (e) or N5 treated with CdCl2 (f). The plasma membrane was labeled with WGA-Rhodamine. Z-stacks at the level of the substratum identifying filopodia or above the substratum identifying TNTs are shown. Treatment of N5 cells with CdCl2 increases both the length of filopodia and the amount of TNTs (white arrows) observed. Quantification of the number of TNTs in Raw 264.7 cells (± CdCl2) (g) or N5 cells (± CdCl2) (h). We observed a decrease in the number of cells with TNTs in Raw 264.7 cells treated with CdCl2 compared to the untreated control cells (g) and a 40% increase in the number of cells connected by TNTs in N5 treated with CdCl2 (h). Data are the average of 4 independent experiments and the graphs show the means (± s.e.m), with a P value <0.01 (**)
    Figure Legend Snippet: Western blot analyses of Nef (a) and Myo10 (c) expression levels in Raw 264.7 cells (1), Raw 264.7 treated with CdCl2 (2), N5 cells (3) and N5 treated with CdCl2 (4). GAPDH and Calnexin were used as loading controls for Nef and Myo10 respectively. Treatment of N5 cells with CdCl2 increases both Nef and Myo10 expression (lanes 3 vs 4). Blots are representative of 4 independent experiments. Densitometry representation of Nef expression (b) or Myo10 expression (d) from 4 independent experiments. Graphs show the means (± s.e.m), with a P value <0.01 (**) or < 0.02 (*). Representative fluorescence images of Raw 264.7 cells treated with CdCl2 (e) or N5 treated with CdCl2 (f). The plasma membrane was labeled with WGA-Rhodamine. Z-stacks at the level of the substratum identifying filopodia or above the substratum identifying TNTs are shown. Treatment of N5 cells with CdCl2 increases both the length of filopodia and the amount of TNTs (white arrows) observed. Quantification of the number of TNTs in Raw 264.7 cells (± CdCl2) (g) or N5 cells (± CdCl2) (h). We observed a decrease in the number of cells with TNTs in Raw 264.7 cells treated with CdCl2 compared to the untreated control cells (g) and a 40% increase in the number of cells connected by TNTs in N5 treated with CdCl2 (h). Data are the average of 4 independent experiments and the graphs show the means (± s.e.m), with a P value <0.01 (**)

    Techniques Used: Western Blot, Expressing, Fluorescence, Labeling

    (a) Western blot analyses of Myo10 and Nef levels in N5-shRNA control cells versus N5-shRNA Myo10 cells. Calnexin was used as a loading control. Blot is a representative of 3 independent experiments. Densitometry representation of Myo10 (b) or Nef expression (c) are plotted. Graphs show the means (± s.e.m), with a P value <0.0001 (***). (d) Quantification of the number of cells with TNTs in N5-shRNA control cells versus N5-shRNA Myo10 cells. Down-regulation of Myo10 results in a decrease in TNT formation, independently of Nef expression. The graph shows the means (± s.e.m), with a P value <0.05 (*)
    Figure Legend Snippet: (a) Western blot analyses of Myo10 and Nef levels in N5-shRNA control cells versus N5-shRNA Myo10 cells. Calnexin was used as a loading control. Blot is a representative of 3 independent experiments. Densitometry representation of Myo10 (b) or Nef expression (c) are plotted. Graphs show the means (± s.e.m), with a P value <0.0001 (***). (d) Quantification of the number of cells with TNTs in N5-shRNA control cells versus N5-shRNA Myo10 cells. Down-regulation of Myo10 results in a decrease in TNT formation, independently of Nef expression. The graph shows the means (± s.e.m), with a P value <0.05 (*)

    Techniques Used: Western Blot, shRNA, Expressing

    (a) CEM-T4 were mixed in a 1 to 3 ratio with N5 shRNA Ctl cells or with N5 shRNA Myo10 cells. Representative 2-D scatter plots of the CD4 fluorescence intensity (log scale) in CEM-T4 mixed with N5 shRNA Myo10 cells (red) vs shRNA control cells (black). A slight upward shift in CD4 fluorescence intensity can be observed. Change in CD4+ mean fluorescence intensity (MFI) of CEM-T4 cells co-cultured with N5-shRNA control cells versus N5-shRNA Myo10 cells. Representative CDF (b) and histogram (c) views are plotted. Dashed, dotted, and gray lines are the same as in Figs. ​Figs.44 and ​and5;5; red lines are CEMT4 cells co-cultured with N5-shRNA Myo10 cells and blue lines are CEMT4 cells co-cultured with N5-shRNA control cells (scramble shRNA). (d) Graphical representation plotting the percent of CEM-T4 cells with a Mean Fluorescence Intensity (MFI) below that of control cells (CD4 labeled CEM-T4) from 3 independent flow cytometry experiments. The increase of cells below MFI of control cells observed in Fig. ​Fig.44 can be significantly reversed by knockdown of Myo10. Flow cytometry data was the average of 5 independent experiments. The graph shows the means (± s.e.m), with a P value <0.001 (***)
    Figure Legend Snippet: (a) CEM-T4 were mixed in a 1 to 3 ratio with N5 shRNA Ctl cells or with N5 shRNA Myo10 cells. Representative 2-D scatter plots of the CD4 fluorescence intensity (log scale) in CEM-T4 mixed with N5 shRNA Myo10 cells (red) vs shRNA control cells (black). A slight upward shift in CD4 fluorescence intensity can be observed. Change in CD4+ mean fluorescence intensity (MFI) of CEM-T4 cells co-cultured with N5-shRNA control cells versus N5-shRNA Myo10 cells. Representative CDF (b) and histogram (c) views are plotted. Dashed, dotted, and gray lines are the same as in Figs. ​Figs.44 and ​and5;5; red lines are CEMT4 cells co-cultured with N5-shRNA Myo10 cells and blue lines are CEMT4 cells co-cultured with N5-shRNA control cells (scramble shRNA). (d) Graphical representation plotting the percent of CEM-T4 cells with a Mean Fluorescence Intensity (MFI) below that of control cells (CD4 labeled CEM-T4) from 3 independent flow cytometry experiments. The increase of cells below MFI of control cells observed in Fig. ​Fig.44 can be significantly reversed by knockdown of Myo10. Flow cytometry data was the average of 5 independent experiments. The graph shows the means (± s.e.m), with a P value <0.001 (***)

    Techniques Used: shRNA, Fluorescence, Cell Culture, Labeling, Flow Cytometry

    Western blot analyses of Myo10 expression in (a) mouse neuronal CAD cells and (b) human epithelial HeLa cells transfected with GFP-vector (control) and Nef-GFP are shown. Calnexin was used as loading controls. Blots are representative of 3 independent experiments, P value = 0.005 (*). In both cell types, Myo10 expression increases upon Nef-GFP expression. Human MDM were infected with reverse transcriptase-normalized VSV-G pseudotyped virus stocks (WT = pNL4–3 clone) or (DelNef = pNL4–3delNef clone) at 5, 10, and 20 cpm of reverse transcriptase activity/cell. (c) A representative Western blot of the WT or DelNef infected cells from 3 independent infections is shown along with (d) Densitometry representation of the relative expression levels of Myo10 normalized to the amount of tubulin (loading control), with a P value <0.01 (**) or < 0.05 (*)
    Figure Legend Snippet: Western blot analyses of Myo10 expression in (a) mouse neuronal CAD cells and (b) human epithelial HeLa cells transfected with GFP-vector (control) and Nef-GFP are shown. Calnexin was used as loading controls. Blots are representative of 3 independent experiments, P value = 0.005 (*). In both cell types, Myo10 expression increases upon Nef-GFP expression. Human MDM were infected with reverse transcriptase-normalized VSV-G pseudotyped virus stocks (WT = pNL4–3 clone) or (DelNef = pNL4–3delNef clone) at 5, 10, and 20 cpm of reverse transcriptase activity/cell. (c) A representative Western blot of the WT or DelNef infected cells from 3 independent infections is shown along with (d) Densitometry representation of the relative expression levels of Myo10 normalized to the amount of tubulin (loading control), with a P value <0.01 (**) or < 0.05 (*)

    Techniques Used: Western Blot, Expressing, Transfection, Plasmid Preparation, Infection, Activity Assay



    Similar Products

    clones myosin x shrna m lentiviral particles  (Santa Cruz Biotechnology)
    90
    Santa Cruz Biotechnology clones myosin x shrna m lentiviral particles
    Western blot analyses of Nef (a) and <t>Myo10</t> (c) expression levels in Raw 264.7 cells (1), Raw 264.7 treated with CdCl2 (2), N5 cells (3) and N5 treated with CdCl2 (4). GAPDH and Calnexin were used as loading controls for Nef and Myo10 respectively. Treatment of N5 cells with CdCl2 increases both Nef and Myo10 expression (lanes 3 vs 4). Blots are representative of 4 independent experiments. Densitometry representation of Nef expression (b) or Myo10 expression (d) from 4 independent experiments. Graphs show the means (± s.e.m), with a P value <0.01 (**) or < 0.02 (*). Representative fluorescence images of Raw 264.7 cells treated with CdCl2 (e) or N5 treated with CdCl2 (f). The plasma membrane was labeled with WGA-Rhodamine. Z-stacks at the level of the substratum identifying filopodia or above the substratum identifying TNTs are shown. Treatment of N5 cells with CdCl2 increases both the length of filopodia and the amount of TNTs (white arrows) observed. Quantification of the number of TNTs in Raw 264.7 cells (± CdCl2) (g) or N5 cells (± CdCl2) (h). We observed a decrease in the number of cells with TNTs in Raw 264.7 cells treated with CdCl2 compared to the untreated control cells (g) and a 40% increase in the number of cells connected by TNTs in N5 treated with CdCl2 (h). Data are the average of 4 independent experiments and the graphs show the means (± s.e.m), with a P value <0.01 (**)
    Clones Myosin X Shrna M Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clones myosin x shrna m lentiviral particles/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    clones myosin x shrna m lentiviral particles - by Bioz Stars, 2025-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Western blot analyses of Nef (a) and Myo10 (c) expression levels in Raw 264.7 cells (1), Raw 264.7 treated with CdCl2 (2), N5 cells (3) and N5 treated with CdCl2 (4). GAPDH and Calnexin were used as loading controls for Nef and Myo10 respectively. Treatment of N5 cells with CdCl2 increases both Nef and Myo10 expression (lanes 3 vs 4). Blots are representative of 4 independent experiments. Densitometry representation of Nef expression (b) or Myo10 expression (d) from 4 independent experiments. Graphs show the means (± s.e.m), with a P value <0.01 (**) or < 0.02 (*). Representative fluorescence images of Raw 264.7 cells treated with CdCl2 (e) or N5 treated with CdCl2 (f). The plasma membrane was labeled with WGA-Rhodamine. Z-stacks at the level of the substratum identifying filopodia or above the substratum identifying TNTs are shown. Treatment of N5 cells with CdCl2 increases both the length of filopodia and the amount of TNTs (white arrows) observed. Quantification of the number of TNTs in Raw 264.7 cells (± CdCl2) (g) or N5 cells (± CdCl2) (h). We observed a decrease in the number of cells with TNTs in Raw 264.7 cells treated with CdCl2 compared to the untreated control cells (g) and a 40% increase in the number of cells connected by TNTs in N5 treated with CdCl2 (h). Data are the average of 4 independent experiments and the graphs show the means (± s.e.m), with a P value <0.01 (**)

    Journal: Journal of Cell Communication and Signaling

    Article Title: Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes

    doi: 10.1007/s12079-018-0493-z

    Figure Lengend Snippet: Western blot analyses of Nef (a) and Myo10 (c) expression levels in Raw 264.7 cells (1), Raw 264.7 treated with CdCl2 (2), N5 cells (3) and N5 treated with CdCl2 (4). GAPDH and Calnexin were used as loading controls for Nef and Myo10 respectively. Treatment of N5 cells with CdCl2 increases both Nef and Myo10 expression (lanes 3 vs 4). Blots are representative of 4 independent experiments. Densitometry representation of Nef expression (b) or Myo10 expression (d) from 4 independent experiments. Graphs show the means (± s.e.m), with a P value <0.01 (**) or < 0.02 (*). Representative fluorescence images of Raw 264.7 cells treated with CdCl2 (e) or N5 treated with CdCl2 (f). The plasma membrane was labeled with WGA-Rhodamine. Z-stacks at the level of the substratum identifying filopodia or above the substratum identifying TNTs are shown. Treatment of N5 cells with CdCl2 increases both the length of filopodia and the amount of TNTs (white arrows) observed. Quantification of the number of TNTs in Raw 264.7 cells (± CdCl2) (g) or N5 cells (± CdCl2) (h). We observed a decrease in the number of cells with TNTs in Raw 264.7 cells treated with CdCl2 compared to the untreated control cells (g) and a 40% increase in the number of cells connected by TNTs in N5 treated with CdCl2 (h). Data are the average of 4 independent experiments and the graphs show the means (± s.e.m), with a P value <0.01 (**)

    Article Snippet: Transduction and selection of stable clones Myosin-X shRNA (m) Lentiviral Particles (Cat # sc-43,242-V), Control shRNA Lentiviral Particles-A (Cat # sc-108,080), Polybrene (Cat # sc-134,220) and puromycin dihydrochloride (Cat # sc-108,071) were purchased from Santa Cruz Biotechnology, Inc., and were used according to the manufacturer’s instructions.

    Techniques: Western Blot, Expressing, Fluorescence, Labeling

    (a) Western blot analyses of Myo10 and Nef levels in N5-shRNA control cells versus N5-shRNA Myo10 cells. Calnexin was used as a loading control. Blot is a representative of 3 independent experiments. Densitometry representation of Myo10 (b) or Nef expression (c) are plotted. Graphs show the means (± s.e.m), with a P value <0.0001 (***). (d) Quantification of the number of cells with TNTs in N5-shRNA control cells versus N5-shRNA Myo10 cells. Down-regulation of Myo10 results in a decrease in TNT formation, independently of Nef expression. The graph shows the means (± s.e.m), with a P value <0.05 (*)

    Journal: Journal of Cell Communication and Signaling

    Article Title: Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes

    doi: 10.1007/s12079-018-0493-z

    Figure Lengend Snippet: (a) Western blot analyses of Myo10 and Nef levels in N5-shRNA control cells versus N5-shRNA Myo10 cells. Calnexin was used as a loading control. Blot is a representative of 3 independent experiments. Densitometry representation of Myo10 (b) or Nef expression (c) are plotted. Graphs show the means (± s.e.m), with a P value <0.0001 (***). (d) Quantification of the number of cells with TNTs in N5-shRNA control cells versus N5-shRNA Myo10 cells. Down-regulation of Myo10 results in a decrease in TNT formation, independently of Nef expression. The graph shows the means (± s.e.m), with a P value <0.05 (*)

    Article Snippet: Transduction and selection of stable clones Myosin-X shRNA (m) Lentiviral Particles (Cat # sc-43,242-V), Control shRNA Lentiviral Particles-A (Cat # sc-108,080), Polybrene (Cat # sc-134,220) and puromycin dihydrochloride (Cat # sc-108,071) were purchased from Santa Cruz Biotechnology, Inc., and were used according to the manufacturer’s instructions.

    Techniques: Western Blot, shRNA, Expressing

    (a) CEM-T4 were mixed in a 1 to 3 ratio with N5 shRNA Ctl cells or with N5 shRNA Myo10 cells. Representative 2-D scatter plots of the CD4 fluorescence intensity (log scale) in CEM-T4 mixed with N5 shRNA Myo10 cells (red) vs shRNA control cells (black). A slight upward shift in CD4 fluorescence intensity can be observed. Change in CD4+ mean fluorescence intensity (MFI) of CEM-T4 cells co-cultured with N5-shRNA control cells versus N5-shRNA Myo10 cells. Representative CDF (b) and histogram (c) views are plotted. Dashed, dotted, and gray lines are the same as in Figs. ​Figs.44 and ​and5;5; red lines are CEMT4 cells co-cultured with N5-shRNA Myo10 cells and blue lines are CEMT4 cells co-cultured with N5-shRNA control cells (scramble shRNA). (d) Graphical representation plotting the percent of CEM-T4 cells with a Mean Fluorescence Intensity (MFI) below that of control cells (CD4 labeled CEM-T4) from 3 independent flow cytometry experiments. The increase of cells below MFI of control cells observed in Fig. ​Fig.44 can be significantly reversed by knockdown of Myo10. Flow cytometry data was the average of 5 independent experiments. The graph shows the means (± s.e.m), with a P value <0.001 (***)

    Journal: Journal of Cell Communication and Signaling

    Article Title: Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes

    doi: 10.1007/s12079-018-0493-z

    Figure Lengend Snippet: (a) CEM-T4 were mixed in a 1 to 3 ratio with N5 shRNA Ctl cells or with N5 shRNA Myo10 cells. Representative 2-D scatter plots of the CD4 fluorescence intensity (log scale) in CEM-T4 mixed with N5 shRNA Myo10 cells (red) vs shRNA control cells (black). A slight upward shift in CD4 fluorescence intensity can be observed. Change in CD4+ mean fluorescence intensity (MFI) of CEM-T4 cells co-cultured with N5-shRNA control cells versus N5-shRNA Myo10 cells. Representative CDF (b) and histogram (c) views are plotted. Dashed, dotted, and gray lines are the same as in Figs. ​Figs.44 and ​and5;5; red lines are CEMT4 cells co-cultured with N5-shRNA Myo10 cells and blue lines are CEMT4 cells co-cultured with N5-shRNA control cells (scramble shRNA). (d) Graphical representation plotting the percent of CEM-T4 cells with a Mean Fluorescence Intensity (MFI) below that of control cells (CD4 labeled CEM-T4) from 3 independent flow cytometry experiments. The increase of cells below MFI of control cells observed in Fig. ​Fig.44 can be significantly reversed by knockdown of Myo10. Flow cytometry data was the average of 5 independent experiments. The graph shows the means (± s.e.m), with a P value <0.001 (***)

    Article Snippet: Transduction and selection of stable clones Myosin-X shRNA (m) Lentiviral Particles (Cat # sc-43,242-V), Control shRNA Lentiviral Particles-A (Cat # sc-108,080), Polybrene (Cat # sc-134,220) and puromycin dihydrochloride (Cat # sc-108,071) were purchased from Santa Cruz Biotechnology, Inc., and were used according to the manufacturer’s instructions.

    Techniques: shRNA, Fluorescence, Cell Culture, Labeling, Flow Cytometry

    Western blot analyses of Myo10 expression in (a) mouse neuronal CAD cells and (b) human epithelial HeLa cells transfected with GFP-vector (control) and Nef-GFP are shown. Calnexin was used as loading controls. Blots are representative of 3 independent experiments, P value = 0.005 (*). In both cell types, Myo10 expression increases upon Nef-GFP expression. Human MDM were infected with reverse transcriptase-normalized VSV-G pseudotyped virus stocks (WT = pNL4–3 clone) or (DelNef = pNL4–3delNef clone) at 5, 10, and 20 cpm of reverse transcriptase activity/cell. (c) A representative Western blot of the WT or DelNef infected cells from 3 independent infections is shown along with (d) Densitometry representation of the relative expression levels of Myo10 normalized to the amount of tubulin (loading control), with a P value <0.01 (**) or < 0.05 (*)

    Journal: Journal of Cell Communication and Signaling

    Article Title: Myosin-X is essential to the intercellular spread of HIV-1 Nef through tunneling nanotubes

    doi: 10.1007/s12079-018-0493-z

    Figure Lengend Snippet: Western blot analyses of Myo10 expression in (a) mouse neuronal CAD cells and (b) human epithelial HeLa cells transfected with GFP-vector (control) and Nef-GFP are shown. Calnexin was used as loading controls. Blots are representative of 3 independent experiments, P value = 0.005 (*). In both cell types, Myo10 expression increases upon Nef-GFP expression. Human MDM were infected with reverse transcriptase-normalized VSV-G pseudotyped virus stocks (WT = pNL4–3 clone) or (DelNef = pNL4–3delNef clone) at 5, 10, and 20 cpm of reverse transcriptase activity/cell. (c) A representative Western blot of the WT or DelNef infected cells from 3 independent infections is shown along with (d) Densitometry representation of the relative expression levels of Myo10 normalized to the amount of tubulin (loading control), with a P value <0.01 (**) or < 0.05 (*)

    Article Snippet: Transduction and selection of stable clones Myosin-X shRNA (m) Lentiviral Particles (Cat # sc-43,242-V), Control shRNA Lentiviral Particles-A (Cat # sc-108,080), Polybrene (Cat # sc-134,220) and puromycin dihydrochloride (Cat # sc-108,071) were purchased from Santa Cruz Biotechnology, Inc., and were used according to the manufacturer’s instructions.

    Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Infection, Activity Assay