5 ethynyl uridine 5 eu  (Jena Bioscience)


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    Name:
    5 Ethynyl uridine 5 EU
    Description:
    Ethynyl labeled uridine 5 EU can be used as a replacement for BrU 5 Bromo uridine to measure de novo RNA synthesis in proliferating cells 5 EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
    Catalog Number:
    clk-n002-10
    Molecular Weight:
    268.22 g/mol
    Price:
    102.9
    Applications:
    RNA synthesis monitoring[1]
    Purity:
    ≥ 99 % (HPLC)
    Category:
    Click Chemistry
    Format:
    white to off-white solid
    Formula:
    C11H12N2O6
    Buy from Supplier


    Structured Review

    Jena Bioscience 5 ethynyl uridine 5 eu
    UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying <t>5-ethynyl-uridine</t> incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.
    Ethynyl labeled uridine 5 EU can be used as a replacement for BrU 5 Bromo uridine to measure de novo RNA synthesis in proliferating cells 5 EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine The resulting ethynyl functionalized RNA can subsequently be detected via Cu I catalyzed click chemistry that offers the choice to introduce a Biotin group via Azides of Biotin for subsequent purification tasks or a fluorescent group via Azides of fluorescent dyes for subsequent microscopic imaging 1 Presolski et al 2 and Hong et al 3 provide a general protocol for Cu I catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays
    https://www.bioz.com/result/5 ethynyl uridine 5 eu/product/Jena Bioscience
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    5 ethynyl uridine 5 eu - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "γH2AX in the S Phase After UV Irradiation Corresponds to the Sites of DNA Replication and Not DNA Damage"

    Article Title: γH2AX in the S Phase After UV Irradiation Corresponds to the Sites of DNA Replication and Not DNA Damage

    Journal: bioRxiv

    doi: 10.1101/810689

    UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying 5-ethynyl-uridine incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.
    Figure Legend Snippet: UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying 5-ethynyl-uridine incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.

    Techniques Used:

    2) Product Images from "Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei"

    Article Title: Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gky1255

    RHS are involved in global mRNA synthesis ( A ) Quantification of 5-ethynyl uridine (5-EU) incorporation. Each RNAi line was cultured in the presence or absence of tetracycline for 2 days and pulsed for 10 min with 5-EU ( n = 3). Values are given as a percentage of the uninduced control. P-values are shown for paired, one-tailed t-tests. Error bars = SD. ( B ) Correlation between reads per million (RPM) for nascent mRNAs from RNAi lines cultured in the presence or absence of tetracycline for 2 days. R: Pearson correlation coefficient. Biological replicates were performed. See also Supplemental Figure S5 .
    Figure Legend Snippet: RHS are involved in global mRNA synthesis ( A ) Quantification of 5-ethynyl uridine (5-EU) incorporation. Each RNAi line was cultured in the presence or absence of tetracycline for 2 days and pulsed for 10 min with 5-EU ( n = 3). Values are given as a percentage of the uninduced control. P-values are shown for paired, one-tailed t-tests. Error bars = SD. ( B ) Correlation between reads per million (RPM) for nascent mRNAs from RNAi lines cultured in the presence or absence of tetracycline for 2 days. R: Pearson correlation coefficient. Biological replicates were performed. See also Supplemental Figure S5 .

    Techniques Used: Cell Culture, One-tailed Test

    3) Product Images from "Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei"

    Article Title: Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gky1255

    RHS are involved in global mRNA synthesis ( A ) Quantification of 5-ethynyl uridine (5-EU) incorporation. Each RNAi line was cultured in the presence or absence of tetracycline for 2 days and pulsed for 10 min with 5-EU ( n = 3). Values are given as a percentage of the uninduced control. P-values are shown for paired, one-tailed t-tests. Error bars = SD. ( B ) Correlation between reads per million (RPM) for nascent mRNAs from RNAi lines cultured in the presence or absence of tetracycline for 2 days. R: Pearson correlation coefficient. Biological replicates were performed. See also Supplemental Figure S5 .
    Figure Legend Snippet: RHS are involved in global mRNA synthesis ( A ) Quantification of 5-ethynyl uridine (5-EU) incorporation. Each RNAi line was cultured in the presence or absence of tetracycline for 2 days and pulsed for 10 min with 5-EU ( n = 3). Values are given as a percentage of the uninduced control. P-values are shown for paired, one-tailed t-tests. Error bars = SD. ( B ) Correlation between reads per million (RPM) for nascent mRNAs from RNAi lines cultured in the presence or absence of tetracycline for 2 days. R: Pearson correlation coefficient. Biological replicates were performed. See also Supplemental Figure S5 .

    Techniques Used: Cell Culture, One-tailed Test

    4) Product Images from "γH2AX in the S Phase After UV Irradiation Corresponds to the Sites of DNA Replication and Not DNA Damage"

    Article Title: γH2AX in the S Phase After UV Irradiation Corresponds to the Sites of DNA Replication and Not DNA Damage

    Journal: bioRxiv

    doi: 10.1101/810689

    UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying 5-ethynyl-uridine incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.
    Figure Legend Snippet: UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying 5-ethynyl-uridine incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.

    Techniques Used:

    Related Articles

    Isolation:

    Article Title: Hormone stimulation of androgen receptor mediates dynamic changes in DNA methylation patterns at regulatory elements
    Article Snippet: .. The cells were pulsed with 0.5 mM 5-ethynyl uridine (5-EU) (Jena Biosciences, Cat #CLK-N002-10) for 30 min before harvesting in 1.0 ml Trizol® reagent for RNA isolation. ..

    Injection:

    Article Title: Early genome activation in Drosophila is extensive with an initial tendency for aborted transcripts and retained introns
    Article Snippet: .. Embryos were injected with either 50 mM or 250 mM 5-ethynyl uridine (Jena Biosciences) using a PLI-100 Plus Pico-Injector (Harvard Apparatus) adjusted such that the injected volume was approximately equal to one-fifth the volume of a single embryo (∼40 psi). ..

    other:

    Article Title: Visualization of the Nucleolus Using Ethynyl Uridine
    Article Snippet: In such cases, the use of CLK-N002-10 product (diluted in water) is recommended.

    Labeling:

    Article Title: Visualization of the Nucleolus Using Ethynyl Uridine
    Article Snippet: .. EU Labeling Two types of EU were used in this study, product CLK-N002-10 (Jena Bioscience, 200 mM in sterile water) and E-10345 (Life Technologies, 100 mM in DMSO). .. Four days old A. thaliana seedlings were transferred into 12-well plates (Greiner Bio-One).

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  • 88
    Jena Bioscience 5 ethynyl uridine 5 eu
    UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying <t>5-ethynyl-uridine</t> incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.
    5 Ethynyl Uridine 5 Eu, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 ethynyl uridine 5 eu/product/Jena Bioscience
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    5 ethynyl uridine 5 eu - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying 5-ethynyl-uridine incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.

    Journal: bioRxiv

    Article Title: γH2AX in the S Phase After UV Irradiation Corresponds to the Sites of DNA Replication and Not DNA Damage

    doi: 10.1101/810689

    Figure Lengend Snippet: UV-induced γH2AX does not report on the extent of DNA damage in the S phase cells (A) Definition of the colocalization metric: more than 50% overlap between the foci from the two channels at a position. Images are of NCS-treated cells: those 53BP1 foci colocalizing with γH2AX foci are considered as DSBs (B) Percentage of γH2AX foci which are double strand breaks for control, UV- and NCS-treated cells. For UV treatment a very little fraction of γH2AX foci in the S phase cells corresponds to DSBs. (C) Global transcription is measured in UV-treated cells by quantifying 5-ethynyl-uridine incorporation. (D) Bar graphs for γH2AX and EU are normalized across the four dosages. Resultant bar graphs for EU are inverted over those for γH2AX such that the total height of the two bars corresponding to control population in every cell cycle phase is unity. A clear gap is observed between γH2AX and EU bars for G1 and G2/M phases after UV treatment. The γH2AX bars in the S phase not just overlap with EU bars but goes past the unit box showing a disproportionate increase in γH2AX. (E) Mean levels of γH2AX increase with the increase in DNA damage as achieved by increasing the UV dosage in all the phases of the cell cycle. For all the dosages, there is a sharp γH2AX peak in the S phase. (F) Increase in DNA damage always leads to decrease in EU as seen across the population treated with different dosages of UV. But within a population there is no dip in EU levels corresponding to the γH2AX peak showing that γH2AX in the S phase after UV does not reflect on the total extent of DNA damage in those cells. Cells were treated with 10 J/m 2 and 1.6 µg/ml NCS for two minutes. Line graphs are showing relative levels with respect to the mean value of G1 phase in control cells. Scale bar: 10 µm.

    Article Snippet: For measuring global transcription, cells were labelled with 5-Ethynyl-Uridine (EU) immediately after UV irradiation for 30 minutes.

    Techniques:

    RHS are involved in global mRNA synthesis ( A ) Quantification of 5-ethynyl uridine (5-EU) incorporation. Each RNAi line was cultured in the presence or absence of tetracycline for 2 days and pulsed for 10 min with 5-EU ( n = 3). Values are given as a percentage of the uninduced control. P-values are shown for paired, one-tailed t-tests. Error bars = SD. ( B ) Correlation between reads per million (RPM) for nascent mRNAs from RNAi lines cultured in the presence or absence of tetracycline for 2 days. R: Pearson correlation coefficient. Biological replicates were performed. See also Supplemental Figure S5 .

    Journal: Nucleic Acids Research

    Article Title: Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei

    doi: 10.1093/nar/gky1255

    Figure Lengend Snippet: RHS are involved in global mRNA synthesis ( A ) Quantification of 5-ethynyl uridine (5-EU) incorporation. Each RNAi line was cultured in the presence or absence of tetracycline for 2 days and pulsed for 10 min with 5-EU ( n = 3). Values are given as a percentage of the uninduced control. P-values are shown for paired, one-tailed t-tests. Error bars = SD. ( B ) Correlation between reads per million (RPM) for nascent mRNAs from RNAi lines cultured in the presence or absence of tetracycline for 2 days. R: Pearson correlation coefficient. Biological replicates were performed. See also Supplemental Figure S5 .

    Article Snippet: 5-Ethynyl uridine incorporation and processing for GRO-Seq Procyclic forms at a density of 7–8 × 106 ml−1 were pulsed for 10 min with 200 μM 5-ethynyl uridine (5-EU; Jena Biosciences).

    Techniques: Cell Culture, One-tailed Test