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![Expression kinetics and subcellular localization of HCMV lncRNAs. a) Expression levels of HCMV encoded lncRNAs (RNA2.7, RNA1.2, RNA4.9 and exonic RNA5.0) together with the median expression of viral genes and one host transcript (ACTB) as measured by RNA-seq during HCMV infection in fibroblasts (MOI = 5) [ 24 ]. b) HCMV lncRNAs were detected by RNA-FISH using fluorescent probes (white) in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 1). c) RNA4.9 and the UL44 protein were detected in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 5) using RNA-FISH and IF, respectively. Differential interference contrast (DIC) of the stained cell shows viral <t>DNA</t> replication compartments, indicated by black arrows. d) RNA4.9 and nascent DNA were detected in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 3) using RNA-FISH and <t>EdU</t> incorporation followed by labelling with a 6-FAM fluorescent azide using the “Click” chemistry. b-d) Nuclei were counterstained with Hoechst (blue, in merge).](https://storage.googleapis.com/bioz_article_images/PMC7185721/ppat.1008390.g001.jpg)
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1) Product Images from "Human cytomegalovirus long noncoding RNA4.9 regulates viral DNA replication"
Article Title: Human cytomegalovirus long noncoding RNA4.9 regulates viral DNA replication
Journal: PLoS Pathogens
doi: 10.1371/journal.ppat.1008390
![... 48 hpi (MOI = 3) using RNA-FISH and EdU incorporation followed by labelling with a 6-FAM fluorescent ... Expression kinetics and subcellular localization of HCMV lncRNAs. a) Expression levels of HCMV encoded lncRNAs (RNA2.7, RNA1.2, RNA4.9 and exonic RNA5.0) together with the median expression of viral genes and one host transcript (ACTB) as measured by RNA-seq during HCMV infection in fibroblasts (MOI = 5) [ 24 ]. b) HCMV lncRNAs were detected by RNA-FISH using fluorescent probes (white) in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 1). c) RNA4.9 and the UL44 protein were detected in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 5) using RNA-FISH and IF, respectively. Differential interference contrast (DIC) of the stained cell shows viral DNA replication compartments, indicated by black arrows. d) RNA4.9 and nascent DNA were detected in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 3) using RNA-FISH and EdU incorporation followed by labelling with a 6-FAM fluorescent azide using the “Click” chemistry. b-d) Nuclei were counterstained with Hoechst (blue, in merge).](https://storage.googleapis.com/bioz_article_images/PMC7185721/ppat.1008390.g001.jpg)
Figure Legend Snippet: Expression kinetics and subcellular localization of HCMV lncRNAs. a) Expression levels of HCMV encoded lncRNAs (RNA2.7, RNA1.2, RNA4.9 and exonic RNA5.0) together with the median expression of viral genes and one host transcript (ACTB) as measured by RNA-seq during HCMV infection in fibroblasts (MOI = 5) [ 24 ]. b) HCMV lncRNAs were detected by RNA-FISH using fluorescent probes (white) in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 1). c) RNA4.9 and the UL44 protein were detected in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 5) using RNA-FISH and IF, respectively. Differential interference contrast (DIC) of the stained cell shows viral DNA replication compartments, indicated by black arrows. d) RNA4.9 and nascent DNA were detected in HCMV Merlin strain-infected fibroblasts at 48 hpi (MOI = 3) using RNA-FISH and EdU incorporation followed by labelling with a 6-FAM fluorescent azide using the “Click” chemistry. b-d) Nuclei were counterstained with Hoechst (blue, in merge).
Techniques Used: Expressing, RNA Sequencing Assay, Infection, Fluorescence In Situ Hybridization, Staining
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