dbco peg 4 nhs ester  (Jena Bioscience)


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    Jena Bioscience dbco peg 4 nhs ester
    Dbco Peg 4 Nhs Ester, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dbco peg 4 nhs ester  (Jena Bioscience)


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    Jena Bioscience dbco peg 4 nhs ester
    Dbco Peg 4 Nhs Ester, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dbco peg 4 nhs ester  (Jena Bioscience)


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    Jena Bioscience dbco peg 4 nhs ester
    Dbco Peg 4 Nhs Ester, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzylcyclooctyne amine  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne amine
    Dibenzylcyclooctyne Amine, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bodipy peg4 dbco 13  (Jena Bioscience)


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    Jena Bioscience bodipy peg4 dbco 13
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    click dye bodipy fl peg4 dbco  (Jena Bioscience)


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    Jena Bioscience click dye bodipy fl peg4 dbco
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    dibenzoazacyclooctyne dbco  (Jena Bioscience)


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    Jena Bioscience dibenzoazacyclooctyne dbco
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    dibenzylcyclooctyne amine  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne amine
    Dibenzylcyclooctyne Amine, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dibenzylcyclooctyne peg 4 nhs ester  (Jena Bioscience)


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    Jena Bioscience dibenzylcyclooctyne peg 4 nhs ester
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    dbco peg 4 maleimide  (Jena Bioscience)


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    Jena Bioscience dbco peg 4 maleimide
    ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.
    Dbco Peg 4 Maleimide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach"

    Article Title: Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach

    Journal: Science Advances

    doi: 10.1126/sciadv.aay5696

    ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.
    Figure Legend Snippet: ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.

    Techniques Used: Labeling, Functional Assay, Imaging, Fluorescence, Protein Concentration, Concentration Assay

    dbco peg4 nhs ester  (Jena Bioscience)


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    Jena Bioscience dbco peg4 nhs ester
    Dbco Peg4 Nhs Ester, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jena Bioscience dbco peg 4 maleimide
    ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.
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    Jena Bioscience dbco peg4 nhs ester
    ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.
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    ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.

    Journal: Science Advances

    Article Title: Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach

    doi: 10.1126/sciadv.aay5696

    Figure Lengend Snippet: ( A ) The heterobifunctional linker DBCO-PEG 4 -maleimide enables covalent labeling of ligands of interest with an oligonucleotide label. A free sulfhydryl group on the protein is reacted first with the maleimide moiety on the cross-linker, introducing a DBCO functional group to the ligand that then reacts via click chemistry to an azide-terminated oligonucleotide. SH, thiol group. ( B ) The incorporation of an oligonucleotide label having a fluorescent tag enables imaging and monitoring of DNA-directed ligand patterns. For proof of concept, eGFP with a Cy5 tag was assembled using DNA surface patterns (top). Trends in fluorescence intensity profiles for the patterned protein and the fluorescent tag closely matched one other when tuning surface DNA concentrations (2, 4, and 10 μM, from left to right), suggesting that the fluorescent oligonucleotide label can also be used as a relative readout of patterned protein concentration (bottom). ( C ) Multicomponent DNA surface patterns enable tunable control over each ligand concentration as evident in the DNA assembly of eGFP and mCherry. mCherry concentration was held constant as eGFP concentration was tuned as quantified by the change in eGFP fluorescence intensity (bottom) and visualized in the fluorescent composite images (top). Surface DNA concentration for mCherry was 20 μM. Surface DNA concentration for eGFP was 1, 2, and 20 μM (left to right). Scale bars, 500 μm.

    Article Snippet: Immediately before use, a 10 mM solution of DBCO-PEG 4 -maleimide (Jena Bioscience) was prepared in anhydrous dimethyl sulfoxide and reacted, at a fourfold molar excess, with the protein of interest (i.e., eGFP, mCherry, or FGF-2) diluted to 0.1 mM in conjugation buffer [1× PBS with 1 mM EDTA (pH 7)].

    Techniques: Labeling, Functional Assay, Imaging, Fluorescence, Protein Concentration, Concentration Assay