dbco af488  (Jena Bioscience)


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    Name:
    DBCO AF488
    Description:

    Catalog Number:
    CLK-1278-1
    Price:
    175.8
    Category:
    Click Chemistry
    Size:
    1 mg
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    Structured Review

    Jena Bioscience dbco af488

    https://www.bioz.com/result/dbco af488/product/Jena Bioscience
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dbco af488 - by Bioz Stars, 2021-05
    95/100 stars

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    Related Articles

    Functional Assay:

    Article Title: Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria
    Article Snippet: .. Afterwards, the cell pellet was resuspended in 100 µl of 5 µM Dibenzocyclooctyne-amine Alexa Fluor 488 (DBCO-AF488; Jena Bioscience, Germany) and the click reactions between the functional azide group of the lipid and the functional DBCO group of the dye took place at 37 °C for 15 min. After washing once with PBS, the pellet was fixed in 4% formaldehyde (in PBS) for 1 h at RT. .. The pellet was washed 5 times in PBS and incubated in ammonium chloride (50 mM ammonium chloride in PBS) for 15 min.

    Incubation:

    Article Title: Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
    Article Snippet: Afterwards, the cells were fixed in 4 % PFA and 0.1 % GA for 15 min, washed 3x in 1xPBS and then permeabilized for 15 min in 0.2 % Triton X-100 in PBS. .. The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) at 37 °C for 30 min. For staining with antibodies, the cells were washed, blocked using 2 % FCS in 1xPBS for 1 h and then incubated in primary antibody diluted in blocking buffer for 1 h in a humid chamber. .. The primary antibodies used in this study were: anti-HSP60 ms (Santa Cruz, sc-57840, dilution 1:200), anti-Neisseria gonorrhoeae primary antibody rb (US biological, dilution 1:200), anti-Prx3 (Origene, TA322470, dilution 1:100) and anti-CERT (Abcam, ab72536, 1:100).

    Article Title: Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
    Article Snippet: Afterwards, the cells were fixed in 4% PFA and 0.1% GA for 15 min, washed 3x in 1xPBS and then permeabilized for 15 min in 0.2% Triton X-100 in PBS. .. The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) at 37 °C for 30 min. For staining with antibodies, the cells were washed, blocked using 2% FCS in 1xPBS for 1 h and then incubated in primary antibody diluted in blocking buffer for 1 h in a humid chamber. .. The primary antibodies used in this study were: anti-HSP60 ms (Santa Cruz, sc-57840, dilution 1:200), anti-Neisseria gonorrhoeae primary antibody rb (US biological, dilution 1:200), anti-Prx3 (Origene, TA322470, dilution 1:100), anti-CERT (Abcam, ab72536, 1:100) and anti-LPS (BioRAD, MCA2718, dilution 1:200).

    Staining:

    Article Title: Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
    Article Snippet: Afterwards, the cells were fixed in 4 % PFA and 0.1 % GA for 15 min, washed 3x in 1xPBS and then permeabilized for 15 min in 0.2 % Triton X-100 in PBS. .. The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) at 37 °C for 30 min. For staining with antibodies, the cells were washed, blocked using 2 % FCS in 1xPBS for 1 h and then incubated in primary antibody diluted in blocking buffer for 1 h in a humid chamber. .. The primary antibodies used in this study were: anti-HSP60 ms (Santa Cruz, sc-57840, dilution 1:200), anti-Neisseria gonorrhoeae primary antibody rb (US biological, dilution 1:200), anti-Prx3 (Origene, TA322470, dilution 1:100) and anti-CERT (Abcam, ab72536, 1:100).

    Article Title: Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
    Article Snippet: Afterwards, the cells were fixed in 4% PFA and 0.1% GA for 15 min, washed 3x in 1xPBS and then permeabilized for 15 min in 0.2% Triton X-100 in PBS. .. The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) at 37 °C for 30 min. For staining with antibodies, the cells were washed, blocked using 2% FCS in 1xPBS for 1 h and then incubated in primary antibody diluted in blocking buffer for 1 h in a humid chamber. .. The primary antibodies used in this study were: anti-HSP60 ms (Santa Cruz, sc-57840, dilution 1:200), anti-Neisseria gonorrhoeae primary antibody rb (US biological, dilution 1:200), anti-Prx3 (Origene, TA322470, dilution 1:100), anti-CERT (Abcam, ab72536, 1:100) and anti-LPS (BioRAD, MCA2718, dilution 1:200).

    Blocking Assay:

    Article Title: Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
    Article Snippet: Afterwards, the cells were fixed in 4 % PFA and 0.1 % GA for 15 min, washed 3x in 1xPBS and then permeabilized for 15 min in 0.2 % Triton X-100 in PBS. .. The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) at 37 °C for 30 min. For staining with antibodies, the cells were washed, blocked using 2 % FCS in 1xPBS for 1 h and then incubated in primary antibody diluted in blocking buffer for 1 h in a humid chamber. .. The primary antibodies used in this study were: anti-HSP60 ms (Santa Cruz, sc-57840, dilution 1:200), anti-Neisseria gonorrhoeae primary antibody rb (US biological, dilution 1:200), anti-Prx3 (Origene, TA322470, dilution 1:100) and anti-CERT (Abcam, ab72536, 1:100).

    Article Title: Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
    Article Snippet: Afterwards, the cells were fixed in 4% PFA and 0.1% GA for 15 min, washed 3x in 1xPBS and then permeabilized for 15 min in 0.2% Triton X-100 in PBS. .. The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) at 37 °C for 30 min. For staining with antibodies, the cells were washed, blocked using 2% FCS in 1xPBS for 1 h and then incubated in primary antibody diluted in blocking buffer for 1 h in a humid chamber. .. The primary antibodies used in this study were: anti-HSP60 ms (Santa Cruz, sc-57840, dilution 1:200), anti-Neisseria gonorrhoeae primary antibody rb (US biological, dilution 1:200), anti-Prx3 (Origene, TA322470, dilution 1:100), anti-CERT (Abcam, ab72536, 1:100) and anti-LPS (BioRAD, MCA2718, dilution 1:200).

    Infection:

    Article Title: A Role of Sphingosine in the Intracellular Survival of Neisseria gonorrhoeae
    Article Snippet: .. The infection was performed with an MOI of 30 for 4 h. To stop the infection after the indicated infection time, cells were washed three times with Hepes medium and the copper-free clicking reaction with 5 μM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific, Waltham, USA) or 5 μM Alexa Fluor® 488-DBCO (Jena Bioscience, Jena, Germany) was performed for 30 min in a humidified atmosphere at 37°C. ..

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  • 95
    Jena Bioscience dbco af488
    Dbco Af488, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dbco af488/product/Jena Bioscience
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dbco af488 - by Bioz Stars, 2021-05
    95/100 stars
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