thpta  (Jena Bioscience)


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  • 94
    Name:
    THPTA
    Description:

    Catalog Number:
    CLK-1010-100
    Price:
    145.9
    Category:
    Click Chemistry
    Size:
    100 mg
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    Structured Review

    Jena Bioscience thpta

    https://www.bioz.com/result/thpta/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thpta - by Bioz Stars, 2021-04
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Nucleotidyl transferase assisted DNA labeling with different click chemistries
    Article Snippet: All CuAAC reactions were performed in presence of 100 μM CuSO4 , 500 μM Tris-(3-hydroxypropyltriazolylmethyl)-amine (THPTA) ( ) and 1 mM sodium ascorbate at 37°C for 2 h. For SPAAC of DIBAC-containing DNA Cy5 azide (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM 5-DIBAC-dUTP; final concentration in SPAAC: 250 nM DNA, 2.5 μM 5-DIBAC-dUTP) and incubated at 65°C for 20 min. For DARinv Cy5 tetrazine (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM Norb-dA/C/UTP; DARinv : 250 nM DNA, 2.5 μM Norb-dNTP) and incubated at 37°C for 2 h. Azide-containing DNA [DNA2/3, spiked with the respective radiolabeled DNA; TdT-reacted overnight at 2 μM (3′-N3 -ddATP; 5-PEG N3 -dUTP) or 4 μM (2′-N3 -ATP; 8-N3 -ATP; N6 -H N3 -3′-dATP) DNA concentration with 4 μM (5-PEG N3 -dUTP), 10 μM (N6 -H N3 -ATP), 100 μM (2′-N3 -ATP; 8-N3 -ATP) or 1 mM (3′-N3 -ddATP) NTP] was purified (250 nM final) and reacted at variable concentrations of the respective reaction partner (2.5 μM to 2 mM) in CuAAC, SPAAC and Staudinger ligation. .. For CuAAC, DNA was incubated with Alexa Fluor 647 alkyne (Life Technologies), CuSO4 (100 μM), THPTA (500 μM) and sodium ascorbate (1 mM) at 37°C for 2 h, for SPAAC with DIBAC-Fluor-488 (Jena Bioscience) at 65°C for 20 min, and for Staudinger ligation with DyLight 488-phosphine (Thermo Scientific) at 60°C for 2 h and all products were purified by ethanol precipitation. ..

    Ligation:

    Article Title: Nucleotidyl transferase assisted DNA labeling with different click chemistries
    Article Snippet: All CuAAC reactions were performed in presence of 100 μM CuSO4 , 500 μM Tris-(3-hydroxypropyltriazolylmethyl)-amine (THPTA) ( ) and 1 mM sodium ascorbate at 37°C for 2 h. For SPAAC of DIBAC-containing DNA Cy5 azide (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM 5-DIBAC-dUTP; final concentration in SPAAC: 250 nM DNA, 2.5 μM 5-DIBAC-dUTP) and incubated at 65°C for 20 min. For DARinv Cy5 tetrazine (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM Norb-dA/C/UTP; DARinv : 250 nM DNA, 2.5 μM Norb-dNTP) and incubated at 37°C for 2 h. Azide-containing DNA [DNA2/3, spiked with the respective radiolabeled DNA; TdT-reacted overnight at 2 μM (3′-N3 -ddATP; 5-PEG N3 -dUTP) or 4 μM (2′-N3 -ATP; 8-N3 -ATP; N6 -H N3 -3′-dATP) DNA concentration with 4 μM (5-PEG N3 -dUTP), 10 μM (N6 -H N3 -ATP), 100 μM (2′-N3 -ATP; 8-N3 -ATP) or 1 mM (3′-N3 -ddATP) NTP] was purified (250 nM final) and reacted at variable concentrations of the respective reaction partner (2.5 μM to 2 mM) in CuAAC, SPAAC and Staudinger ligation. .. For CuAAC, DNA was incubated with Alexa Fluor 647 alkyne (Life Technologies), CuSO4 (100 μM), THPTA (500 μM) and sodium ascorbate (1 mM) at 37°C for 2 h, for SPAAC with DIBAC-Fluor-488 (Jena Bioscience) at 65°C for 20 min, and for Staudinger ligation with DyLight 488-phosphine (Thermo Scientific) at 60°C for 2 h and all products were purified by ethanol precipitation. ..

    Purification:

    Article Title: Nucleotidyl transferase assisted DNA labeling with different click chemistries
    Article Snippet: All CuAAC reactions were performed in presence of 100 μM CuSO4 , 500 μM Tris-(3-hydroxypropyltriazolylmethyl)-amine (THPTA) ( ) and 1 mM sodium ascorbate at 37°C for 2 h. For SPAAC of DIBAC-containing DNA Cy5 azide (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM 5-DIBAC-dUTP; final concentration in SPAAC: 250 nM DNA, 2.5 μM 5-DIBAC-dUTP) and incubated at 65°C for 20 min. For DARinv Cy5 tetrazine (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM Norb-dA/C/UTP; DARinv : 250 nM DNA, 2.5 μM Norb-dNTP) and incubated at 37°C for 2 h. Azide-containing DNA [DNA2/3, spiked with the respective radiolabeled DNA; TdT-reacted overnight at 2 μM (3′-N3 -ddATP; 5-PEG N3 -dUTP) or 4 μM (2′-N3 -ATP; 8-N3 -ATP; N6 -H N3 -3′-dATP) DNA concentration with 4 μM (5-PEG N3 -dUTP), 10 μM (N6 -H N3 -ATP), 100 μM (2′-N3 -ATP; 8-N3 -ATP) or 1 mM (3′-N3 -ddATP) NTP] was purified (250 nM final) and reacted at variable concentrations of the respective reaction partner (2.5 μM to 2 mM) in CuAAC, SPAAC and Staudinger ligation. .. For CuAAC, DNA was incubated with Alexa Fluor 647 alkyne (Life Technologies), CuSO4 (100 μM), THPTA (500 μM) and sodium ascorbate (1 mM) at 37°C for 2 h, for SPAAC with DIBAC-Fluor-488 (Jena Bioscience) at 65°C for 20 min, and for Staudinger ligation with DyLight 488-phosphine (Thermo Scientific) at 60°C for 2 h and all products were purified by ethanol precipitation. ..

    Ethanol Precipitation:

    Article Title: Nucleotidyl transferase assisted DNA labeling with different click chemistries
    Article Snippet: All CuAAC reactions were performed in presence of 100 μM CuSO4 , 500 μM Tris-(3-hydroxypropyltriazolylmethyl)-amine (THPTA) ( ) and 1 mM sodium ascorbate at 37°C for 2 h. For SPAAC of DIBAC-containing DNA Cy5 azide (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM 5-DIBAC-dUTP; final concentration in SPAAC: 250 nM DNA, 2.5 μM 5-DIBAC-dUTP) and incubated at 65°C for 20 min. For DARinv Cy5 tetrazine (25 μM) was added to non-purified TdT mixture (TdT-reaction: 1 μM DNA, 10 μM Norb-dA/C/UTP; DARinv : 250 nM DNA, 2.5 μM Norb-dNTP) and incubated at 37°C for 2 h. Azide-containing DNA [DNA2/3, spiked with the respective radiolabeled DNA; TdT-reacted overnight at 2 μM (3′-N3 -ddATP; 5-PEG N3 -dUTP) or 4 μM (2′-N3 -ATP; 8-N3 -ATP; N6 -H N3 -3′-dATP) DNA concentration with 4 μM (5-PEG N3 -dUTP), 10 μM (N6 -H N3 -ATP), 100 μM (2′-N3 -ATP; 8-N3 -ATP) or 1 mM (3′-N3 -ddATP) NTP] was purified (250 nM final) and reacted at variable concentrations of the respective reaction partner (2.5 μM to 2 mM) in CuAAC, SPAAC and Staudinger ligation. .. For CuAAC, DNA was incubated with Alexa Fluor 647 alkyne (Life Technologies), CuSO4 (100 μM), THPTA (500 μM) and sodium ascorbate (1 mM) at 37°C for 2 h, for SPAAC with DIBAC-Fluor-488 (Jena Bioscience) at 65°C for 20 min, and for Staudinger ligation with DyLight 488-phosphine (Thermo Scientific) at 60°C for 2 h and all products were purified by ethanol precipitation. ..

    Centrifugation:

    Article Title: Inhibition of the ubiquitin-proteasome system by a bioactivatable compound
    Article Snippet: CLICK chemistry and analytical labellingCells were washed with PBS and the pellets lysed in lysis buffer (1% NP-40, 100 mM NaCl, 50 mM HEPES pH 7.2). .. Following centrifugation (10 min, 16,000× g, 4°C), supernatants were supplemented with 50 µM TAMRA-azide (Jena Bioscience), 5 mM sodium ascorbate (Sigma-Aldrich), 5 mM aminoguanidine-HCl (Sigma-Aldrich), 0.1 mM CuSO4 (Sigma-Aldrich) and 0.5 mM THPTA (Jena Bioscience), with gentle vortexing. .. The reaction mixtures were incubated at RT for 30 min, light protected.

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  • 94
    Jena Bioscience thpta
    Thpta, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thpta/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thpta - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

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