Journal: Journal of Proteome Research

Article Title: Glycoproteomics Identifies Plexin-B3 as a Targetable Cell Surface Protein Required for the Growth and Invasion of Triple-Negative Breast Cancer Cells

doi: 10.1021/acs.jproteome.2c00332

Figure Lengend Snippet: List of Antibodies Used for Western Blotting Experiments

Article Snippet: cleaved-CASP7 , rabbit , M.C.; clone D6H1 , cell signaling , 8438 , 1:1000.

Techniques: Western Blot

Journal: International Journal of Molecular Sciences

Article Title: PRKAR1B-AS2 Long Noncoding RNA Promotes Tumorigenesis, Survival, and Chemoresistance via the PI3K/AKT/mTOR Pathway

doi: 10.3390/ijms22041882

Figure Lengend Snippet: List of antibodies used.

Article Snippet: Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb , Cell Signaling Technology, Danvers, MA, USA (8438) , 1:400 , Anti-rabbit IgG, HRP-linked Antibody , Cell Signaling Technology, Danvers, MA, USA (7074) , 1:2000.

Techniques:

Journal: Biomolecules

Article Title: A Hydroxypropyl Methylcellulose-Based Solid Dispersion of Curcumin with Enhanced Bioavailability and Its Hepatoprotective Activity

doi: 10.3390/biom9070281

Figure Lengend Snippet: Effects of DW-CUR 20 on cleavage of caspases-7, -8 and PARP in HepG2 cells. Curcumin (4 µg/mL) was used as a positive control. Whole cell lysates were separated by electrophoresis, transferred onto membranes, and probed with the indicated antibodies. Cleaved caspase-7, -8 and PARP were detected using specific primary antibodies. β-Actin served as an internal loading control. All immunoblot bands were obtained from the same cell lysates ( a ). The bar charts display the intensity of Cleaved caspase-7, -8 or PARP after being normalized by β-Actin using ImageJ software ( b – d ). Data are presented as the means ± SD of three independent experiments. *** p < 0.001 vs the t-BHP treatment group.

Article Snippet: Primary antibodies against cleaved caspase-7 (D6H1) and Bcl-2 (D17C4) were purchased from Cell Signaling Technology (Denver, MA, USA).

Techniques: Positive Control, Electrophoresis, Western Blot, Software

Journal: PLoS ONE

Article Title: HCV Induces Telomerase Reverse Transcriptase, Increases Its Catalytic Activity, and Promotes Caspase Degradation in Infected Human Hepatocytes

doi: 10.1371/journal.pone.0166853

Figure Lengend Snippet: (A) Left panel, log phase Huh 5.15NS replicons were incubated with the pancaspase inhibitor (z-vad-fmk) (200uM) and at various times cellular lysates were assayed on WB for TERT or NS3-4A using C-terminal specific antibody. In right panel, log phase Huh 5.15NS replicons were incubated with indicated amounts of the specific Caspase 7 inhibitor (Millipore 28832) for 48 hr. Cellular lysates were then assayed on WB for TERT using C-terminal specific antibody. In the lower panel, log phase Huh 5.15NS replicons were incubated with pancaspase inhibitor or vehicle control for the indicated times and relative telomerase activity was determined in the cellular lysates as compared to day 0 using Real-time TRAP-RT PCR. *[Pancaspase inhibitor > control days 2, 3, and 4, p< 0.01] (B) Huh-7.5 cells were either infected with HCVcc (right panel) or mock control (left panel) and on various days WB were performed to detect TERT, NS3, and un-activated full length caspases 6 and 7 as well as cleaved fragments as indicated. # Antibodies recognizing both full length and upper cleaved caspase fragments were from Cell Signaling (Caspase 7 #9492 and Caspase 6 #9762). (C) Huh-7.5 or Huh-7 cells were infected with HCVcc and on various days assayed for TERT as well as lower kD cleaved fragments of caspase 6 and 7 by WB. Cell Signaling *ASP 162 antibody (#9761) was used to detect cleaved caspase 6 and **ASP 198 antibody (#8438) was used to detect cleaved caspase 7 with products appearing at 18 kD in both cases.

Article Snippet: All anti-caspase antibodies were obtained from Cell Signaling : Anti-caspase-6 antibody (#9762, polyclonal rabbit antibody, 1:1000 dilution), caspase-7 antibody (#9492, rabbit polyclonal, 1:1000 dilution), cleaved caspase-6 (Asp162) antibody (#9761, rabbit polyclonal, 1:1000 dilution), and cleaved caspase-7 (Asp198) (D6H1) antibody (#8438, rabbit monoclonal 1:1000 dilution) .

Techniques: Incubation, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Infection