cleaved caspase 3 rabbit 9664s ab 10831820 cst  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3 rabbit 9664s ab 10831820 cst
    Cleaved Caspase 3 Rabbit 9664s Ab 10831820 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3 rabbit 9664s ab 10831820 cst/product/Cell Signaling Technology Inc
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    3 cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 3 cleaved caspase 3
    3 Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti rabbit cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti rabbit cleaved caspase 3
    Anti Rabbit Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit cleaved caspase 3/product/Cell Signaling Technology Inc
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    rabbit cleaved caspase 3 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit cleaved caspase 3 antibody
    Rabbit Cleaved Caspase 3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit cleaved caspase 3 antibody/product/Cell Signaling Technology Inc
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    anti rabbit cleaved caspase 3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti rabbit cleaved caspase 3
    ERp57 protects against mutant TDP-43 induced cell death in neuronal cell lines A Neuro-2a cells were co-expressed with wild-type TDP-43 (TDP-WT) or TDP-43 M337V (M337V, green) and V5 tagged ERp57 (red), examined by confocal microscopy at 72 h post transfection. Nuclei are shown by Hoechst stain (blue). Arrow represents condensed or fragmented nuclei, indicating apoptosis is underway. Few cells expressing TDP-WT (panel 1) or TDP-WT co-expressing ERp57 (panel 2) contained fragmented nuclei and hence were apoptotic (< 1%) but more cells expressing TDP-43 M337V (panel 3) displayed Hoechst-stained condensed nuclei, indicating apoptosis, indicated by white arrows (middle panel). However, fewer cells co-expressing TDP-43 M337V with ERp57 (panel 3) were undergoing apoptosis compared to those transfected with empty vector, scale bar = 5 µm. B Quantification of apoptotic nuclei in cells in 5A expressing TDP-43 and ERp57. Results are expressed as mean ± SD, n = 3. A significant difference in apoptosis was observed between wild-type TDP-43 and TDP-43 M337V cells (****p < 0.0001). Over-expression of ERp57 with TDP-43 M337V resulted in significantly fewer cells undergoing apoptosis compared to cells transfected with empty vector only (***p < 0.001). C <t>Activated</t> <t>caspase-3</t> immunoreactivity, confirming induction of apoptosis, in cells expressing TDP-43 M337V and ERp57. Neuro-2a cells were co-expressed with either wild-type TDP-43 or TDP-43 M337V (green) and ERp57 for 72 h, followed by immunocytochemistry using anti-activated caspase-3 antibodies (red), visualized using confocal microscopy. Nuclei are shown by Hoechst stain (blue). White arrow represents caspase-3 activation, indicating apoptosis is underway. As expected, fewer cells expressing wild-type TDP-43 (row 1) displayed caspase-3 activation, compared to cells expressing TDP-43 M337V (row 3). However, fewer cells expressing TDP-43 M337V with ERp57 (row 4) displayed caspase-3 activation, compared to those TDP-43 M337V cells transfected with empty vector. D Quantification of transfected cells visualized in 6C, immunostained using anti-activated caspase-3 antibodies. Results are expressed as mean ± SD, n = 3. Over-expression of ERp57 with TDP-43 M337V significantly decreased the proportion of cells with activated caspase-3, indicating apoptotic cell death is underway, compared to cells expressing empty vector only (**p < 0.01, ***p<0.001 )
    Anti Rabbit Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Protein Disulfide Isomerase Endoplasmic Reticulum Protein 57 (ERp57) is Protective Against ALS-Associated Mutant TDP-43 in Neuronal Cells"

    Article Title: Protein Disulfide Isomerase Endoplasmic Reticulum Protein 57 (ERp57) is Protective Against ALS-Associated Mutant TDP-43 in Neuronal Cells

    Journal: Neuromolecular Medicine

    doi: 10.1007/s12017-024-08787-0

    ERp57 protects against mutant TDP-43 induced cell death in neuronal cell lines A Neuro-2a cells were co-expressed with wild-type TDP-43 (TDP-WT) or TDP-43 M337V (M337V, green) and V5 tagged ERp57 (red), examined by confocal microscopy at 72 h post transfection. Nuclei are shown by Hoechst stain (blue). Arrow represents condensed or fragmented nuclei, indicating apoptosis is underway. Few cells expressing TDP-WT (panel 1) or TDP-WT co-expressing ERp57 (panel 2) contained fragmented nuclei and hence were apoptotic (< 1%) but more cells expressing TDP-43 M337V (panel 3) displayed Hoechst-stained condensed nuclei, indicating apoptosis, indicated by white arrows (middle panel). However, fewer cells co-expressing TDP-43 M337V with ERp57 (panel 3) were undergoing apoptosis compared to those transfected with empty vector, scale bar = 5 µm. B Quantification of apoptotic nuclei in cells in 5A expressing TDP-43 and ERp57. Results are expressed as mean ± SD, n = 3. A significant difference in apoptosis was observed between wild-type TDP-43 and TDP-43 M337V cells (****p < 0.0001). Over-expression of ERp57 with TDP-43 M337V resulted in significantly fewer cells undergoing apoptosis compared to cells transfected with empty vector only (***p < 0.001). C Activated caspase-3 immunoreactivity, confirming induction of apoptosis, in cells expressing TDP-43 M337V and ERp57. Neuro-2a cells were co-expressed with either wild-type TDP-43 or TDP-43 M337V (green) and ERp57 for 72 h, followed by immunocytochemistry using anti-activated caspase-3 antibodies (red), visualized using confocal microscopy. Nuclei are shown by Hoechst stain (blue). White arrow represents caspase-3 activation, indicating apoptosis is underway. As expected, fewer cells expressing wild-type TDP-43 (row 1) displayed caspase-3 activation, compared to cells expressing TDP-43 M337V (row 3). However, fewer cells expressing TDP-43 M337V with ERp57 (row 4) displayed caspase-3 activation, compared to those TDP-43 M337V cells transfected with empty vector. D Quantification of transfected cells visualized in 6C, immunostained using anti-activated caspase-3 antibodies. Results are expressed as mean ± SD, n = 3. Over-expression of ERp57 with TDP-43 M337V significantly decreased the proportion of cells with activated caspase-3, indicating apoptotic cell death is underway, compared to cells expressing empty vector only (**p < 0.01, ***p<0.001 )
    Figure Legend Snippet: ERp57 protects against mutant TDP-43 induced cell death in neuronal cell lines A Neuro-2a cells were co-expressed with wild-type TDP-43 (TDP-WT) or TDP-43 M337V (M337V, green) and V5 tagged ERp57 (red), examined by confocal microscopy at 72 h post transfection. Nuclei are shown by Hoechst stain (blue). Arrow represents condensed or fragmented nuclei, indicating apoptosis is underway. Few cells expressing TDP-WT (panel 1) or TDP-WT co-expressing ERp57 (panel 2) contained fragmented nuclei and hence were apoptotic (< 1%) but more cells expressing TDP-43 M337V (panel 3) displayed Hoechst-stained condensed nuclei, indicating apoptosis, indicated by white arrows (middle panel). However, fewer cells co-expressing TDP-43 M337V with ERp57 (panel 3) were undergoing apoptosis compared to those transfected with empty vector, scale bar = 5 µm. B Quantification of apoptotic nuclei in cells in 5A expressing TDP-43 and ERp57. Results are expressed as mean ± SD, n = 3. A significant difference in apoptosis was observed between wild-type TDP-43 and TDP-43 M337V cells (****p < 0.0001). Over-expression of ERp57 with TDP-43 M337V resulted in significantly fewer cells undergoing apoptosis compared to cells transfected with empty vector only (***p < 0.001). C Activated caspase-3 immunoreactivity, confirming induction of apoptosis, in cells expressing TDP-43 M337V and ERp57. Neuro-2a cells were co-expressed with either wild-type TDP-43 or TDP-43 M337V (green) and ERp57 for 72 h, followed by immunocytochemistry using anti-activated caspase-3 antibodies (red), visualized using confocal microscopy. Nuclei are shown by Hoechst stain (blue). White arrow represents caspase-3 activation, indicating apoptosis is underway. As expected, fewer cells expressing wild-type TDP-43 (row 1) displayed caspase-3 activation, compared to cells expressing TDP-43 M337V (row 3). However, fewer cells expressing TDP-43 M337V with ERp57 (row 4) displayed caspase-3 activation, compared to those TDP-43 M337V cells transfected with empty vector. D Quantification of transfected cells visualized in 6C, immunostained using anti-activated caspase-3 antibodies. Results are expressed as mean ± SD, n = 3. Over-expression of ERp57 with TDP-43 M337V significantly decreased the proportion of cells with activated caspase-3, indicating apoptotic cell death is underway, compared to cells expressing empty vector only (**p < 0.01, ***p<0.001 )

    Techniques Used: Mutagenesis, Confocal Microscopy, Transfection, Staining, Expressing, Plasmid Preparation, Over Expression, Immunocytochemistry, Activation Assay

    alexa fluor 647 cleaved caspase 3 rabbit mab asp175  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc alexa fluor 647 cleaved caspase 3 rabbit mab asp175
    Neuro-2A cultures were infected with dLAT2903, McKrae, and ΔsncRNA1&2 viruses (10 pfu/cell for 24 hr) or mock infected. ( A) Detection of cleaved <t>caspase-3</t> in infected cells . At 24 hr PI, cells were harvested and reacted with anti-cleaved capase-3 antibody and FACS analysis was performed for expression of caspase-3 (see ). Experiments were repeated twice; and ( B) Quantification of FACS analysis from A . Percent of cleaved capase-3 positive cells for each infected or mock infected cells were counted. Each bar represents the mean ± SEM of cleaved capase-3-positive cells (N = 6).
    Alexa Fluor 647 Cleaved Caspase 3 Rabbit Mab Asp175, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 cleaved caspase 3 rabbit mab asp175/product/Cell Signaling Technology Inc
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    Images

    1) Product Images from "The anti-apoptotic function of HSV-1 LAT in neuronal cell cultures but not its function during reactivation correlates with expression of two small non-coding RNAs, sncRNA1&2"

    Article Title: The anti-apoptotic function of HSV-1 LAT in neuronal cell cultures but not its function during reactivation correlates with expression of two small non-coding RNAs, sncRNA1&2

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1012307

    Neuro-2A cultures were infected with dLAT2903, McKrae, and ΔsncRNA1&2 viruses (10 pfu/cell for 24 hr) or mock infected. ( A) Detection of cleaved caspase-3 in infected cells . At 24 hr PI, cells were harvested and reacted with anti-cleaved capase-3 antibody and FACS analysis was performed for expression of caspase-3 (see ). Experiments were repeated twice; and ( B) Quantification of FACS analysis from A . Percent of cleaved capase-3 positive cells for each infected or mock infected cells were counted. Each bar represents the mean ± SEM of cleaved capase-3-positive cells (N = 6).
    Figure Legend Snippet: Neuro-2A cultures were infected with dLAT2903, McKrae, and ΔsncRNA1&2 viruses (10 pfu/cell for 24 hr) or mock infected. ( A) Detection of cleaved caspase-3 in infected cells . At 24 hr PI, cells were harvested and reacted with anti-cleaved capase-3 antibody and FACS analysis was performed for expression of caspase-3 (see ). Experiments were repeated twice; and ( B) Quantification of FACS analysis from A . Percent of cleaved capase-3 positive cells for each infected or mock infected cells were counted. Each bar represents the mean ± SEM of cleaved capase-3-positive cells (N = 6).

    Techniques Used: Infection, Expressing

    cleaved caspase 3 primary antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cleaved caspase 3 primary antibody
    4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved <t>caspase</t> <t>3</t> in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.
    Cleaved Caspase 3 Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3 primary antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Targeting cellular stress in vitro improves osteoblast homeostasis, matrix collagen content and mineralization in two murine models of osteogenesis imperfecta"

    Article Title: Targeting cellular stress in vitro improves osteoblast homeostasis, matrix collagen content and mineralization in two murine models of osteogenesis imperfecta

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    doi: 10.1016/j.matbio.2021.03.001

    4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved caspase 3 in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.
    Figure Legend Snippet: 4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved caspase 3 in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.

    Techniques Used: Expressing, Cell Culture, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Transmission Assay, Electron Microscopy, Immunofluorescence, Quantitation Assay, Activation Assay, Marker, Staining

    Schematic view of the mechanisms activated by OI OBs to face intracellular collagen retention and highlighting the molecular mechanisms of action of 4-PBA. (A) Mutated collagen I is partly secreted in the ECM, where it impairs its structure and functions, and partly retained in the ER, causing ER stress and the consequent activation of UPR through protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) and inositol-requiring enzyme 1 α (IRE1α) branches. Phosphorylated PERK activates its effector ATF4, that translocates to the nucleus where it regulates gene expression. IRE1a activation leads to the alternative splicing of its effector X-box binding protein 1 (Xbp1), that works as a transcriptional activator in its spliced form. Intracellular protein accumulation also stimulates the degradative pathway autophagy, as revealed by the increased expression of its terminal marker LC3, present on autophagosome (AP) membrane. Prolonged activation of UPR induces apoptosis, in particular through the cleavage of the main apoptotic executor Caspase 3. A hallmark of apoptosis activation is the flipping of the phospholipid phosphatidylserine (indicated by yellow dots in the cell membrane) from the inner leaflet of the plasma membrane to the outer one. (B) 4-PBA reduces the activation of UPR and apoptosis markers and stimulates general protein secretion, clearing the ER from accumulated material. The drug exerts its effect on collagen, specifically, by promoting its secretion and incorporation into the ECM. Increased Osx and Alp expression following treatment, as well as increased ALP activity, denote 4-PBA stimulatory effect on OBs differentiation and mineralization activity.
    Figure Legend Snippet: Schematic view of the mechanisms activated by OI OBs to face intracellular collagen retention and highlighting the molecular mechanisms of action of 4-PBA. (A) Mutated collagen I is partly secreted in the ECM, where it impairs its structure and functions, and partly retained in the ER, causing ER stress and the consequent activation of UPR through protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) and inositol-requiring enzyme 1 α (IRE1α) branches. Phosphorylated PERK activates its effector ATF4, that translocates to the nucleus where it regulates gene expression. IRE1a activation leads to the alternative splicing of its effector X-box binding protein 1 (Xbp1), that works as a transcriptional activator in its spliced form. Intracellular protein accumulation also stimulates the degradative pathway autophagy, as revealed by the increased expression of its terminal marker LC3, present on autophagosome (AP) membrane. Prolonged activation of UPR induces apoptosis, in particular through the cleavage of the main apoptotic executor Caspase 3. A hallmark of apoptosis activation is the flipping of the phospholipid phosphatidylserine (indicated by yellow dots in the cell membrane) from the inner leaflet of the plasma membrane to the outer one. (B) 4-PBA reduces the activation of UPR and apoptosis markers and stimulates general protein secretion, clearing the ER from accumulated material. The drug exerts its effect on collagen, specifically, by promoting its secretion and incorporation into the ECM. Increased Osx and Alp expression following treatment, as well as increased ALP activity, denote 4-PBA stimulatory effect on OBs differentiation and mineralization activity.

    Techniques Used: Activation Assay, Expressing, Alternative Splicing, Binding Assay, Marker, Membrane, Activity Assay

    cleaved caspase 3 primary antibody  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cleaved caspase 3 primary antibody
    4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved <t>caspase</t> <t>3</t> in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.
    Cleaved Caspase 3 Primary Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3 primary antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 primary antibody - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Targeting cellular stress in vitro improves osteoblast homeostasis, matrix collagen content and mineralization in two murine models of osteogenesis imperfecta"

    Article Title: Targeting cellular stress in vitro improves osteoblast homeostasis, matrix collagen content and mineralization in two murine models of osteogenesis imperfecta

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    doi: 10.1016/j.matbio.2021.03.001

    4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved caspase 3 in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.
    Figure Legend Snippet: 4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved caspase 3 in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.

    Techniques Used: Expressing, Cell Culture, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Transmission Assay, Electron Microscopy, Immunofluorescence, Quantitation Assay, Activation Assay, Marker, Staining

    Schematic view of the mechanisms activated by OI OBs to face intracellular collagen retention and highlighting the molecular mechanisms of action of 4-PBA. (A) Mutated collagen I is partly secreted in the ECM, where it impairs its structure and functions, and partly retained in the ER, causing ER stress and the consequent activation of UPR through protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) and inositol-requiring enzyme 1 α (IRE1α) branches. Phosphorylated PERK activates its effector ATF4, that translocates to the nucleus where it regulates gene expression. IRE1a activation leads to the alternative splicing of its effector X-box binding protein 1 (Xbp1), that works as a transcriptional activator in its spliced form. Intracellular protein accumulation also stimulates the degradative pathway autophagy, as revealed by the increased expression of its terminal marker LC3, present on autophagosome (AP) membrane. Prolonged activation of UPR induces apoptosis, in particular through the cleavage of the main apoptotic executor Caspase 3. A hallmark of apoptosis activation is the flipping of the phospholipid phosphatidylserine (indicated by yellow dots in the cell membrane) from the inner leaflet of the plasma membrane to the outer one. (B) 4-PBA reduces the activation of UPR and apoptosis markers and stimulates general protein secretion, clearing the ER from accumulated material. The drug exerts its effect on collagen, specifically, by promoting its secretion and incorporation into the ECM. Increased Osx and Alp expression following treatment, as well as increased ALP activity, denote 4-PBA stimulatory effect on OBs differentiation and mineralization activity.
    Figure Legend Snippet: Schematic view of the mechanisms activated by OI OBs to face intracellular collagen retention and highlighting the molecular mechanisms of action of 4-PBA. (A) Mutated collagen I is partly secreted in the ECM, where it impairs its structure and functions, and partly retained in the ER, causing ER stress and the consequent activation of UPR through protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) and inositol-requiring enzyme 1 α (IRE1α) branches. Phosphorylated PERK activates its effector ATF4, that translocates to the nucleus where it regulates gene expression. IRE1a activation leads to the alternative splicing of its effector X-box binding protein 1 (Xbp1), that works as a transcriptional activator in its spliced form. Intracellular protein accumulation also stimulates the degradative pathway autophagy, as revealed by the increased expression of its terminal marker LC3, present on autophagosome (AP) membrane. Prolonged activation of UPR induces apoptosis, in particular through the cleavage of the main apoptotic executor Caspase 3. A hallmark of apoptosis activation is the flipping of the phospholipid phosphatidylserine (indicated by yellow dots in the cell membrane) from the inner leaflet of the plasma membrane to the outer one. (B) 4-PBA reduces the activation of UPR and apoptosis markers and stimulates general protein secretion, clearing the ER from accumulated material. The drug exerts its effect on collagen, specifically, by promoting its secretion and incorporation into the ECM. Increased Osx and Alp expression following treatment, as well as increased ALP activity, denote 4-PBA stimulatory effect on OBs differentiation and mineralization activity.

    Techniques Used: Activation Assay, Expressing, Alternative Splicing, Binding Assay, Marker, Membrane, Activity Assay

    cleaved caspase 3 asp175 9661 antibodies  (Cell Signaling Technology Inc)


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    Neuro-2A cultures were infected with dLAT2903, McKrae, and ΔsncRNA1&2 viruses (10 pfu/cell for 24 hr) or mock infected. ( A) Detection of cleaved <t>caspase-3</t> in infected cells . At 24 hr PI, cells were harvested and reacted with anti-cleaved capase-3 antibody and FACS analysis was performed for expression of caspase-3 (see ). Experiments were repeated twice; and ( B) Quantification of FACS analysis from A . Percent of cleaved capase-3 positive cells for each infected or mock infected cells were counted. Each bar represents the mean ± SEM of cleaved capase-3-positive cells (N = 6).
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    Cleaved Caspase 3 Asp175 9661 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved <t>caspase</t> <t>3</t> in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.
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    Neuro-2A cultures were infected with dLAT2903, McKrae, and ΔsncRNA1&2 viruses (10 pfu/cell for 24 hr) or mock infected. ( A) Detection of cleaved caspase-3 in infected cells . At 24 hr PI, cells were harvested and reacted with anti-cleaved capase-3 antibody and FACS analysis was performed for expression of caspase-3 (see ). Experiments were repeated twice; and ( B) Quantification of FACS analysis from A . Percent of cleaved capase-3 positive cells for each infected or mock infected cells were counted. Each bar represents the mean ± SEM of cleaved capase-3-positive cells (N = 6).

    Journal: PLOS Pathogens

    Article Title: The anti-apoptotic function of HSV-1 LAT in neuronal cell cultures but not its function during reactivation correlates with expression of two small non-coding RNAs, sncRNA1&2

    doi: 10.1371/journal.ppat.1012307

    Figure Lengend Snippet: Neuro-2A cultures were infected with dLAT2903, McKrae, and ΔsncRNA1&2 viruses (10 pfu/cell for 24 hr) or mock infected. ( A) Detection of cleaved caspase-3 in infected cells . At 24 hr PI, cells were harvested and reacted with anti-cleaved capase-3 antibody and FACS analysis was performed for expression of caspase-3 (see ). Experiments were repeated twice; and ( B) Quantification of FACS analysis from A . Percent of cleaved capase-3 positive cells for each infected or mock infected cells were counted. Each bar represents the mean ± SEM of cleaved capase-3-positive cells (N = 6).

    Article Snippet: At 24 hr PI, cells were harvested, washed, resuspended in FACS buffer and incubated for 15 min at 4°C with purified 2.4G2 antibody (Fc block, BD Biosciences, San Diego, CA) followed by subsequent incubation with Alexa Fluor 647 cleaved caspase-3 Rabbit mAb {Asp175 (D3E9)}, Cell Signaling Technology, Danvers, MA) at 4°C for 1 hr followed by fixation with BD Cytofix/Cytoperm solution for 20 min at 4°C.

    Techniques: Infection, Expressing

    4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved caspase 3 in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    Article Title: Targeting cellular stress in vitro improves osteoblast homeostasis, matrix collagen content and mineralization in two murine models of osteogenesis imperfecta

    doi: 10.1016/j.matbio.2021.03.001

    Figure Lengend Snippet: 4-PBA ameliorates Brtl and Amish osteoblasts homeostasis. (A) qPCR-based array heat map of relative expression of genes involved in UPR, autophagic and apoptotic pathways in Brtl and Amish OBs in the absence (−) or presence (+) of 4-PBA. Results are expressed as fold differences compared to control. 4-PBA mainly reduced or normalized the upregulated genes. *p<0.05 4-PBA treated Brtl VS control; # p<0.05 4-PBA treated Amish VS control. (B) qPCR analysis of Serpinh1 expression in mutated and control OBs cultured in the absence (−) or presence (+) of 4-PBA. The drug reduced Serpinh1 expression in all samples. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated. (C) RT-PCR amplification of Xbp1 mRNA from control and Brtl untreated (−) and 4-PBA (+) treated OBs. Xbp1-s is reduced by 4-PBA. cDNA from OBs treated with the stress inducing compound tunicamycin were used as positive control for ER stress. (D) Transmission electron microscopy representative images of OI OBs in absence (−) or presence (+) of 4-PBA. The analyses revealed ER enlargement (arrowhead) in mutant cells that was rescued by 4-PBA treatment.Scale bar: 2 μm. (E) Representative immunofluorescence images and quantification of cleaved caspase 3 in WT and mutant OBs in absence (−) or presence (+) of 4-PBA. Quantitation of the total area of punctate signal indicated increased cleaved caspase 3 level in mutant OBs, pointing to the activation of apoptosis. 4-PBA treatment reduced the apoptotic marker signal in mutated cells. Nuclei were stained with DAPI. Scale bar: 2 μm. *p<0.05 WT VS mutant, # p<0.05 4-PBA treated VS untreated.

    Article Snippet: LC3 primary antibody (Cell Signaling) and cleaved caspase 3 primary antibody (Cell Signaling) were used at 1:500 dilution in 1% BSA, 0.3% TritonX100 in PBS and the incubation was carried out o/n at 4 °C.

    Techniques: Expressing, Cell Culture, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Transmission Assay, Electron Microscopy, Immunofluorescence, Quantitation Assay, Activation Assay, Marker, Staining

    Schematic view of the mechanisms activated by OI OBs to face intracellular collagen retention and highlighting the molecular mechanisms of action of 4-PBA. (A) Mutated collagen I is partly secreted in the ECM, where it impairs its structure and functions, and partly retained in the ER, causing ER stress and the consequent activation of UPR through protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) and inositol-requiring enzyme 1 α (IRE1α) branches. Phosphorylated PERK activates its effector ATF4, that translocates to the nucleus where it regulates gene expression. IRE1a activation leads to the alternative splicing of its effector X-box binding protein 1 (Xbp1), that works as a transcriptional activator in its spliced form. Intracellular protein accumulation also stimulates the degradative pathway autophagy, as revealed by the increased expression of its terminal marker LC3, present on autophagosome (AP) membrane. Prolonged activation of UPR induces apoptosis, in particular through the cleavage of the main apoptotic executor Caspase 3. A hallmark of apoptosis activation is the flipping of the phospholipid phosphatidylserine (indicated by yellow dots in the cell membrane) from the inner leaflet of the plasma membrane to the outer one. (B) 4-PBA reduces the activation of UPR and apoptosis markers and stimulates general protein secretion, clearing the ER from accumulated material. The drug exerts its effect on collagen, specifically, by promoting its secretion and incorporation into the ECM. Increased Osx and Alp expression following treatment, as well as increased ALP activity, denote 4-PBA stimulatory effect on OBs differentiation and mineralization activity.

    Journal: Matrix biology : journal of the International Society for Matrix Biology

    Article Title: Targeting cellular stress in vitro improves osteoblast homeostasis, matrix collagen content and mineralization in two murine models of osteogenesis imperfecta

    doi: 10.1016/j.matbio.2021.03.001

    Figure Lengend Snippet: Schematic view of the mechanisms activated by OI OBs to face intracellular collagen retention and highlighting the molecular mechanisms of action of 4-PBA. (A) Mutated collagen I is partly secreted in the ECM, where it impairs its structure and functions, and partly retained in the ER, causing ER stress and the consequent activation of UPR through protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) and inositol-requiring enzyme 1 α (IRE1α) branches. Phosphorylated PERK activates its effector ATF4, that translocates to the nucleus where it regulates gene expression. IRE1a activation leads to the alternative splicing of its effector X-box binding protein 1 (Xbp1), that works as a transcriptional activator in its spliced form. Intracellular protein accumulation also stimulates the degradative pathway autophagy, as revealed by the increased expression of its terminal marker LC3, present on autophagosome (AP) membrane. Prolonged activation of UPR induces apoptosis, in particular through the cleavage of the main apoptotic executor Caspase 3. A hallmark of apoptosis activation is the flipping of the phospholipid phosphatidylserine (indicated by yellow dots in the cell membrane) from the inner leaflet of the plasma membrane to the outer one. (B) 4-PBA reduces the activation of UPR and apoptosis markers and stimulates general protein secretion, clearing the ER from accumulated material. The drug exerts its effect on collagen, specifically, by promoting its secretion and incorporation into the ECM. Increased Osx and Alp expression following treatment, as well as increased ALP activity, denote 4-PBA stimulatory effect on OBs differentiation and mineralization activity.

    Article Snippet: LC3 primary antibody (Cell Signaling) and cleaved caspase 3 primary antibody (Cell Signaling) were used at 1:500 dilution in 1% BSA, 0.3% TritonX100 in PBS and the incubation was carried out o/n at 4 °C.

    Techniques: Activation Assay, Expressing, Alternative Splicing, Binding Assay, Marker, Membrane, Activity Assay