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p putida atcc 12633 def1 clc f psy strain  (ATCC)
97
ATCC p putida atcc 12633 def1 clc f psy strain
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
P Putida Atcc 12633 Def1 Clc F Psy Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp clc hs00171342 m1  (Thermo Fisher)
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Thermo Fisher gene exp clc hs00171342 m1
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Gene Exp Clc Hs00171342 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clc genomic workbench v8.0.1  (Qiagen)
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Qiagen clc genomic workbench v8.0.1
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomic Workbench V8.0.1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clc genomic workbench v8.0.1 - by Bioz Stars, 2026-02
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clc genomics workbench v8.0.1  (Qiagen)
90
Qiagen clc genomics workbench v8.0.1
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomics Workbench V8.0.1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clc genomics workbench v8.0.1/product/Qiagen
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clc genomics workbench v8.0.1 - by Bioz Stars, 2026-02
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clc genomics workbench version 8.5  (Qiagen)
90
Qiagen clc genomics workbench version 8.5
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomics Workbench Version 8.5, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clc genomics workbench version 8.5/product/Qiagen
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clc genomics workbench version 8.5 - by Bioz Stars, 2026-02
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clc genomics workbench  (Qiagen)
90
Qiagen clc genomics workbench
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomics Workbench, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti clc 2  (Alomone Labs)
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Alomone Labs anti clc 2
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Anti Clc 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti clc 2 - by Bioz Stars, 2026-02
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clc genomic work-bench 23.0.1  (Qiagen)
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Qiagen clc genomic work-bench 23.0.1
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomic Work Bench 23.0.1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clc genomics workbench 23.0.1  (Qiagen)
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Qiagen clc genomics workbench 23.0.1
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomics Workbench 23.0.1, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clc genomics workbench 23.0.1/product/Qiagen
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clc genomics workbench 23.0.1 - by Bioz Stars, 2026-02
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clc genomics workbench software  (Qiagen)
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Qiagen clc genomics workbench software
Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, <t>DEF1.</t> The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.
Clc Genomics Workbench Software, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clc genomics workbench software/product/Qiagen
Average 90 stars, based on 1 article reviews
clc genomics workbench software - by Bioz Stars, 2026-02
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Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, DEF1. The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.

Journal: mBio

Article Title: Matrix-independent screening of defluorination in vitro and in vivo

doi: 10.1128/mbio.01798-25

Figure Lengend Snippet: Measuring substrate defluorination in vivo by pH change using a recombinant Pseudomonas expressing a defluorinase, DEF1. The reactions and cell growth were monitored by changes in bromothymol blue absorbance at 615 nm and turbidity at 700 nm (OD 700 ), respectively. The blue curves show the change in pH as a drop in absorbance at 615 nm when cells are present. The magenta curve shows absorbance at 615 nm with substrate alone, which controlled for the dissolution of carbon dioxide from the atmosphere, which gives background acidification. The orange curve shows the increase in OD 700 due to cell growth. ( A ) Reactions and growth with α-fluorophenylacetic acid. ( B ) Reactions and growth with 2-fluoropropionic acid. ( C ) Comparison of the rates of defluorination measured in a previous study with purified enzyme in vitro with the in vivo rate determined here via acidification by the change in absorbance at 615 nm. The rate was determined from the linear regions of the blue curves. The background absorbance change (magenta curve) was subtracted to correct for the control pH change. The rates were multiplied by 10 to make the numeric comparison easier. Error bars shown in parts A and B represent standard deviations from triplicate determinations.

Article Snippet: The P. putida ATCC 12633 DEF1 + CLC F psy strain was grown overnight to the stationary phase in LB with antibiotics as described above.

Techniques: In Vivo, Recombinant, Expressing, Dissolution, Comparison, Purification, In Vitro, Control