Journal: Molecules and Cells
Article Title: Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3
Figure Lengend Snippet: Expression and localization of ClC-2 and ClC-3 in BMDMs. (A) ClC transcripts detected in BMDMs by RTPCR. Heart tissue was used as positive control. (B) ClC protein expression in BMDMs measured by Western blot. Heart tissue served as positive control. For specific controls, antibodies against ClCs were pre-incubated with corresponding peptide antigens. (C) Localization of ClC-3 in BMDMs shown by double-staining immunofluorescence (original magnification × 1000). Scale bar = 20 μm. Nuclei (blue) and ClC-3 (Green) were counterstained with Hoechst 33342 and ClC-3 antibody, respectively. (D, E) ClC-3 expression in BMDMs after hypo-tonic exposure examined by Western blot. Photos taken from a representative experiment. The data represent mean ± SD from three independent experiments. (F) Localization of ClC-3 in BMDMs stimulated with hypotonic solution visualized by double-staining immunofluorescence (original magnification × 1000). Scale bar = 20 μm. ClC-3 collected in clusters around the cytomembrane, shown by arrows (n = 3 for each group).
Article Snippet: Rabbit anti-ClC-3 or rabbit anti-ClC-2 Abs were from Alomone Laboratories (Israel).
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Western Blot, Incubation, Double Immunofluorescence Staining