acl 002  (Alomone Labs)


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    Structured Review

    Alomone Labs acl 002
    Acl 002, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acl 002/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acl 002 - by Bioz Stars, 2022-08
    92/100 stars

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    Alomone Labs clc 2 antibody
    Inhibition of inward-rectifier Cl − currents by <t>ClC-2</t> antibody
    Clc 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clc 2 antibody/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    clc 2 antibody - by Bioz Stars, 2022-08
    90/100 stars
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    Inhibition of inward-rectifier Cl − currents by ClC-2 antibody

    Journal: Biochemical and biophysical research communications

    Article Title: Inward-rectifier chloride currents in Reissner's membrane epithelial cells

    doi: 10.1016/j.bbrc.2010.03.048

    Figure Lengend Snippet: Inhibition of inward-rectifier Cl − currents by ClC-2 antibody

    Article Snippet: ClC-2 antibody against an intracellular domain was obtained from Alomone Labs.

    Techniques: Inhibition

    Functional expression of the ClC-2 channel in submandibular granular duct cells. A : differential interference contrast images of single granular duct and acinar cells freshly isolated from male mouse submandibular glands. Scale bar = 10 μm

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Clcn2 encodes the hyperpolarization-activated chloride channel in the ducts of mouse salivary glands

    doi: 10.1152/ajpgi.90384.2008

    Figure Lengend Snippet: Functional expression of the ClC-2 channel in submandibular granular duct cells. A : differential interference contrast images of single granular duct and acinar cells freshly isolated from male mouse submandibular glands. Scale bar = 10 μm

    Article Snippet: This antibody recognizes the COOH-terminus epitope RSRHGLPREGTPSDSDDKC of rat ClC-2 that is identical to mouse ClC-2 (ACL-002, Alomone Labs, Jerusalem, Israel).

    Techniques: Functional Assay, Expressing, Isolation

    ClC-2 distribution in mouse submandibular and parotid glands. A : immunoperoxidase labeling for ClC-2 in the submandibular gland of a Clcn2 +/+ male mouse. AC, acinar cells; GD, granular duct; SD, striated duct; ED, excretory duct. Inset,

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Clcn2 encodes the hyperpolarization-activated chloride channel in the ducts of mouse salivary glands

    doi: 10.1152/ajpgi.90384.2008

    Figure Lengend Snippet: ClC-2 distribution in mouse submandibular and parotid glands. A : immunoperoxidase labeling for ClC-2 in the submandibular gland of a Clcn2 +/+ male mouse. AC, acinar cells; GD, granular duct; SD, striated duct; ED, excretory duct. Inset,

    Article Snippet: This antibody recognizes the COOH-terminus epitope RSRHGLPREGTPSDSDDKC of rat ClC-2 that is identical to mouse ClC-2 (ACL-002, Alomone Labs, Jerusalem, Israel).

    Techniques: Labeling

    Expression and localization of ClC-2 and ClC-3 in BMDMs. (A) ClC transcripts detected in BMDMs by RTPCR. Heart tissue was used as positive control. (B) ClC protein expression in BMDMs measured by Western blot. Heart tissue served as positive control. For specific controls, antibodies against ClCs were pre-incubated with corresponding peptide antigens. (C) Localization of ClC-3 in BMDMs shown by double-staining immunofluorescence (original magnification × 1000). Scale bar = 20 μm. Nuclei (blue) and ClC-3 (Green) were counterstained with Hoechst 33342 and ClC-3 antibody, respectively. (D, E) ClC-3 expression in BMDMs after hypo-tonic exposure examined by Western blot. Photos taken from a representative experiment. The data represent mean ± SD from three independent experiments. (F) Localization of ClC-3 in BMDMs stimulated with hypotonic solution visualized by double-staining immunofluorescence (original magnification × 1000). Scale bar = 20 μm. ClC-3 collected in clusters around the cytomembrane, shown by arrows (n = 3 for each group).

    Journal: Molecules and Cells

    Article Title: Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3

    doi: 10.14348/molcells.2014.0031

    Figure Lengend Snippet: Expression and localization of ClC-2 and ClC-3 in BMDMs. (A) ClC transcripts detected in BMDMs by RTPCR. Heart tissue was used as positive control. (B) ClC protein expression in BMDMs measured by Western blot. Heart tissue served as positive control. For specific controls, antibodies against ClCs were pre-incubated with corresponding peptide antigens. (C) Localization of ClC-3 in BMDMs shown by double-staining immunofluorescence (original magnification × 1000). Scale bar = 20 μm. Nuclei (blue) and ClC-3 (Green) were counterstained with Hoechst 33342 and ClC-3 antibody, respectively. (D, E) ClC-3 expression in BMDMs after hypo-tonic exposure examined by Western blot. Photos taken from a representative experiment. The data represent mean ± SD from three independent experiments. (F) Localization of ClC-3 in BMDMs stimulated with hypotonic solution visualized by double-staining immunofluorescence (original magnification × 1000). Scale bar = 20 μm. ClC-3 collected in clusters around the cytomembrane, shown by arrows (n = 3 for each group).

    Article Snippet: Rabbit anti-ClC-3 or rabbit anti-ClC-2 Abs were from Alomone Laboratories (Israel).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Western Blot, Incubation, Double Immunofluorescence Staining