citrobacter rodentium  (ATCC)


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    Name:
    Citrobacter rodentium DBS100
    Description:

    Catalog Number:
    51459
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    Applications:
    Characterization
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    Structured Review

    ATCC citrobacter rodentium
    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with <t>Citrobacter</t> <t>rodentium</t> (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    https://www.bioz.com/result/citrobacter rodentium/product/ATCC
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    citrobacter rodentium - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling"

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    Journal: FEMS immunology and medical microbiology

    doi: 10.1111/j.1574-695X.2012.00978.x

    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Figure Legend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Techniques Used: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium
    Figure Legend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Techniques Used: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination
    Figure Legend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Techniques Used: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)
    Figure Legend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Techniques Used: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed
    Figure Legend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Techniques Used: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)
    Figure Legend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Techniques Used: Activation Assay, Mouse Assay

    2) Product Images from "Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection"

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004410

    Enhanced inflammasome activation in Nod2 −/− and Rip2 −/− BMDMs. BMDM were generated from WT, Nod2 −/− and Rip2 −/− mice and infected with 20MOI of Citrobacter rodentium for 18 h. (A–F) Combined supernatant and lysates were examined by Western blot for caspase-1 cleavage (casp-1p20) visually (A,D) and by densitometry (B,E), or (C,F) supernatants were examined for IL-18 secretion by ELISA. (G) BMDM were infected with C. rodentium and assayed for intracellular growth at the indicated times post-infection. (A–F) Data are representative of five independent experiments with n = 2–3 wells per experiment. (G) Data are representative of two independent experiments with n = 2–3 wells per experiment. (B,C,E,F,G) Data are shown as the mean ± SEM. (*, p
    Figure Legend Snippet: Enhanced inflammasome activation in Nod2 −/− and Rip2 −/− BMDMs. BMDM were generated from WT, Nod2 −/− and Rip2 −/− mice and infected with 20MOI of Citrobacter rodentium for 18 h. (A–F) Combined supernatant and lysates were examined by Western blot for caspase-1 cleavage (casp-1p20) visually (A,D) and by densitometry (B,E), or (C,F) supernatants were examined for IL-18 secretion by ELISA. (G) BMDM were infected with C. rodentium and assayed for intracellular growth at the indicated times post-infection. (A–F) Data are representative of five independent experiments with n = 2–3 wells per experiment. (G) Data are representative of two independent experiments with n = 2–3 wells per experiment. (B,C,E,F,G) Data are shown as the mean ± SEM. (*, p

    Techniques Used: Activation Assay, Generated, Mouse Assay, Infection, Western Blot, Enzyme-linked Immunosorbent Assay

    3) Product Images from "Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling"

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    Journal: FEMS immunology and medical microbiology

    doi: 10.1111/j.1574-695X.2012.00978.x

    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Figure Legend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Techniques Used: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium
    Figure Legend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Techniques Used: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination
    Figure Legend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Techniques Used: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)
    Figure Legend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Techniques Used: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed
    Figure Legend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Techniques Used: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)
    Figure Legend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Techniques Used: Activation Assay, Mouse Assay

    4) Product Images from "Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves"

    Article Title: Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves. Cloning and characterization of short‐chain N‐acyl homoserine lactone‐producing Enterobacter asburiae strain L1 from lettuce leaves

    Journal: MicrobiologyOpen

    doi: 10.1002/mbo3.610

    Phylogenetic tree showing the evolutionary distances between the putative AHL synthase, EasI of strain L1 (red word) with the other E. asburiae strains, generated using neighbor‐joining algorithm. The tree is drawn to scale, with branch lengths to show the evolutionary distances in the phylogenetic tree. The horizontal bar indicates evolutionary distance as 0.02 change per nucleotide position. The bootstrap values as percentage of 1,000 replications are given as numbers at the nodes. The LuxI homolog of Citrobacter rodentium is used as an outgroup
    Figure Legend Snippet: Phylogenetic tree showing the evolutionary distances between the putative AHL synthase, EasI of strain L1 (red word) with the other E. asburiae strains, generated using neighbor‐joining algorithm. The tree is drawn to scale, with branch lengths to show the evolutionary distances in the phylogenetic tree. The horizontal bar indicates evolutionary distance as 0.02 change per nucleotide position. The bootstrap values as percentage of 1,000 replications are given as numbers at the nodes. The LuxI homolog of Citrobacter rodentium is used as an outgroup

    Techniques Used: Generated

    5) Product Images from "Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling"

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    Journal: FEMS immunology and medical microbiology

    doi: 10.1111/j.1574-695X.2012.00978.x

    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Figure Legend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Techniques Used: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium
    Figure Legend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Techniques Used: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination
    Figure Legend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Techniques Used: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)
    Figure Legend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Techniques Used: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed
    Figure Legend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Techniques Used: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)
    Figure Legend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Techniques Used: Activation Assay, Mouse Assay

    6) Product Images from "The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection"

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection

    Journal: Nature immunology

    doi: 10.1038/ni.3118

    IRF1 is not required for canonical or non-canonical NLRP3 or NLRC4 inflammasome activation ( a ) Caspase-1 activation, ( b ) IL-1β and IL-18 release and cell death in LPS-primed BMDMs stimulated with ATP or nigericin or in unprimed BMDMs infected with Citrobacter rodentium (MOI 20) or ΔfliCΔfljB mutant S. Typhimurium (MOI 20) or wild-type S. Typhimurium ( S Tm) for 20 h. ( c ) Caspase-1 activation, ( d ) IL-1β and IL-18 release and cell death in unprimed BMDMs infected with log-phase grown S. Typhimurium (MOI 1) for 4 h. Graphs show mean and s.e.m. of three independent experiments. NS, not significant (two-tailed t -test).
    Figure Legend Snippet: IRF1 is not required for canonical or non-canonical NLRP3 or NLRC4 inflammasome activation ( a ) Caspase-1 activation, ( b ) IL-1β and IL-18 release and cell death in LPS-primed BMDMs stimulated with ATP or nigericin or in unprimed BMDMs infected with Citrobacter rodentium (MOI 20) or ΔfliCΔfljB mutant S. Typhimurium (MOI 20) or wild-type S. Typhimurium ( S Tm) for 20 h. ( c ) Caspase-1 activation, ( d ) IL-1β and IL-18 release and cell death in unprimed BMDMs infected with log-phase grown S. Typhimurium (MOI 1) for 4 h. Graphs show mean and s.e.m. of three independent experiments. NS, not significant (two-tailed t -test).

    Techniques Used: Activation Assay, Infection, Mutagenesis, Two Tailed Test

    7) Product Images from "Infection with enteric pathogens Salmonella typhimurium and Citrobacter rodentium modulate TGF-beta/Smad signaling pathways in the intestine"

    Article Title: Infection with enteric pathogens Salmonella typhimurium and Citrobacter rodentium modulate TGF-beta/Smad signaling pathways in the intestine

    Journal: Gut Microbes

    doi: 10.1080/19490976.2018.1429878

    Smad 2/3 Immunostaining in response to Citrobacter infection. OCT embedded tissue sections of representative region of the colon were fixed and stained for total Smad2/3 (alexa fluor 488, green) and Villin (alexa fluor 568, red). A decrease in immunofluorescence staining of Smad2 and Smad3 proteins in the distal colon tissues is shown following C. rodentium infection in representative images. (scale bar: 50 μm).
    Figure Legend Snippet: Smad 2/3 Immunostaining in response to Citrobacter infection. OCT embedded tissue sections of representative region of the colon were fixed and stained for total Smad2/3 (alexa fluor 488, green) and Villin (alexa fluor 568, red). A decrease in immunofluorescence staining of Smad2 and Smad3 proteins in the distal colon tissues is shown following C. rodentium infection in representative images. (scale bar: 50 μm).

    Techniques Used: Immunostaining, Infection, Staining, Immunofluorescence

    Citrobacter rodentium infection decreases colonic mucosal TGF-R1 and TGF-R2 mRNA levels and downregulates Smad2 and Smad3 levels. Streptomycin-pretreated FVB/N mice were colonized with C. rodentium or control for 6 days. RNA extracted from mouse colonic mucosa was amplified using gene specific primers for real-time PCR quantification. Data represent the relative mRNA expression of TGF-R1 (A), TGF-RII (B), Smad2 (C) and Smad3 (D) normalized to the respective GAPDH mRNA (internal control) levels. Data are expressed as mean ± SEM. *p 0.05; **p
    Figure Legend Snippet: Citrobacter rodentium infection decreases colonic mucosal TGF-R1 and TGF-R2 mRNA levels and downregulates Smad2 and Smad3 levels. Streptomycin-pretreated FVB/N mice were colonized with C. rodentium or control for 6 days. RNA extracted from mouse colonic mucosa was amplified using gene specific primers for real-time PCR quantification. Data represent the relative mRNA expression of TGF-R1 (A), TGF-RII (B), Smad2 (C) and Smad3 (D) normalized to the respective GAPDH mRNA (internal control) levels. Data are expressed as mean ± SEM. *p 0.05; **p

    Techniques Used: Infection, Mouse Assay, Amplification, Real-time Polymerase Chain Reaction, Expressing

    Related Articles

    Sequencing:

    Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP
    Article Snippet: .. Draft genome sequence of Citrobacter rodentium DBS100 (ATCC 51459), a primary model of enterohemorrhagic Escherichia coli virulence . ..

    Infection:

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling
    Article Snippet: .. Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma. ..

    Mouse Assay:

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling
    Article Snippet: .. Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma. ..

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    ATCC citrobacter rodentium
    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with <t>Citrobacter</t> <t>rodentium</t> (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Citrobacter Rodentium, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/citrobacter rodentium/product/ATCC
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    citrobacter rodentium - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    95
    ATCC c rodentium
    C. <t>rodentium</t> adjuvant (CRA) induces a milder EAE disease course. C57Bl/6 mice were immunized with MOG35-55. (A) MOG35-55 was given in emulsion with CFA (2mg/ml) or CRA at 2mg/ml, 1mg/ml, and 0.5mg/ml with co-injection of Ptx. Mice were monitored for EAE
    C Rodentium, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c rodentium/product/ATCC
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    c rodentium - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Article Snippet: Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma.

    Techniques: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Article Snippet: Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma.

    Techniques: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Article Snippet: Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma.

    Techniques: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Article Snippet: Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma.

    Techniques: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Article Snippet: Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma.

    Techniques: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Article Snippet: Impaired resistance and enhanced pathology during infection with a noninvasive, attaching-effacing enteric bacterial pathogen, Citrobacter rodentium , in mice lacking IL-12 or IFN-gamma.

    Techniques: Activation Assay, Mouse Assay

    C. rodentium adjuvant (CRA) induces a milder EAE disease course. C57Bl/6 mice were immunized with MOG35-55. (A) MOG35-55 was given in emulsion with CFA (2mg/ml) or CRA at 2mg/ml, 1mg/ml, and 0.5mg/ml with co-injection of Ptx. Mice were monitored for EAE

    Journal: Journal of neuroimmunology

    Article Title: Comparison of a Classical Th1 Bacteria Versus a Th17 Bacteria as Adjuvant in the Induction of Experimental Autoimmune Encephalomyelitis

    doi: 10.1016/j.jneuroim.2011.05.012

    Figure Lengend Snippet: C. rodentium adjuvant (CRA) induces a milder EAE disease course. C57Bl/6 mice were immunized with MOG35-55. (A) MOG35-55 was given in emulsion with CFA (2mg/ml) or CRA at 2mg/ml, 1mg/ml, and 0.5mg/ml with co-injection of Ptx. Mice were monitored for EAE

    Article Snippet: To determine if C. rodentium could induce EAE in a more physiological-relevant model, C57Bl/6 mice were immunized with MOG35-55 in emulsion with CFA or CRA, and Ptx was also given in this and all subsequent experiments.

    Techniques: Mouse Assay, Injection

    C. rodentium adjuvant (CRA) induces a late onset of EAE. (A) B10.PL Vβ8.2 TCR-transgenic mice were immunized with MBP Ac1-11 in emulsion with CFA (2mg/ml), CRA (2mg/ml), or IFA with or without co-injection of pertussis toxin (Ptx). Mice were monitored

    Journal: Journal of neuroimmunology

    Article Title: Comparison of a Classical Th1 Bacteria Versus a Th17 Bacteria as Adjuvant in the Induction of Experimental Autoimmune Encephalomyelitis

    doi: 10.1016/j.jneuroim.2011.05.012

    Figure Lengend Snippet: C. rodentium adjuvant (CRA) induces a late onset of EAE. (A) B10.PL Vβ8.2 TCR-transgenic mice were immunized with MBP Ac1-11 in emulsion with CFA (2mg/ml), CRA (2mg/ml), or IFA with or without co-injection of pertussis toxin (Ptx). Mice were monitored

    Article Snippet: To determine if C. rodentium could induce EAE in a more physiological-relevant model, C57Bl/6 mice were immunized with MOG35-55 in emulsion with CFA or CRA, and Ptx was also given in this and all subsequent experiments.

    Techniques: Transgenic Assay, Mouse Assay, Immunofluorescence, Injection

    PepT1 transgenic mice exhibit decreased susceptibility to C. rodentium infection compared to WT mice WT and PepT1 transgenic mice were infected with C. rodentium and euthanized at 7 days post-infection. ( A ) C. rodentium colonization levels in the colon. ( B ) Neutrophil infiltration into the colon quantified by measuring MPO activity. ( C-i ) Representative H E-stained distal colonic sections. Original magnification, ×20. ( C-ii ) Neutrophils were identified by their distinctive nuclear morphology and counted in 10 crypts per colon. The mean number of neutrophils per crypt was calculated for each colon observed. ( D-i ) KC mRNA levels in the colon determined by real-time RT-PCR. ( D-ii ) KC levels in serum determined by ELISA. Colonic mRNA levels of pro-inflammatory ( E ) and anti-inflammatory ( F ) cytokines determined by real-time RT-PCR. Data are means ± S.E.M. * P

    Journal: Gastroenterology

    Article Title: Pathogenic bacteria induce colonic PepT1 expression: an implication in host defense response

    doi: 10.1053/j.gastro.2009.06.043

    Figure Lengend Snippet: PepT1 transgenic mice exhibit decreased susceptibility to C. rodentium infection compared to WT mice WT and PepT1 transgenic mice were infected with C. rodentium and euthanized at 7 days post-infection. ( A ) C. rodentium colonization levels in the colon. ( B ) Neutrophil infiltration into the colon quantified by measuring MPO activity. ( C-i ) Representative H E-stained distal colonic sections. Original magnification, ×20. ( C-ii ) Neutrophils were identified by their distinctive nuclear morphology and counted in 10 crypts per colon. The mean number of neutrophils per crypt was calculated for each colon observed. ( D-i ) KC mRNA levels in the colon determined by real-time RT-PCR. ( D-ii ) KC levels in serum determined by ELISA. Colonic mRNA levels of pro-inflammatory ( E ) and anti-inflammatory ( F ) cytokines determined by real-time RT-PCR. Data are means ± S.E.M. * P

    Article Snippet: Consistent with these ex vivo data, colonization of C. rodentium in colons from PepT1 transgenic mice was reduced compared to those from WT mice ( ).

    Techniques: Transgenic Assay, Mouse Assay, Infection, Activity Assay, Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    PepT1 overexpression reduces C. rodentium adherence and C. rodentium -induced cytokine production in cultured mouse colons Cultured colonic tissues from WT and PepT1 transgenic mice were infected ex vivo with C. rodentium for 6 h. ( A ) C. rodentium adherence to cultured colonic tissues. KC mRNA and protein levels were determined by real-time RT-PCR ( B ) and ELISA ( C ), respectively. Data are means ± S.E.M. * P

    Journal: Gastroenterology

    Article Title: Pathogenic bacteria induce colonic PepT1 expression: an implication in host defense response

    doi: 10.1053/j.gastro.2009.06.043

    Figure Lengend Snippet: PepT1 overexpression reduces C. rodentium adherence and C. rodentium -induced cytokine production in cultured mouse colons Cultured colonic tissues from WT and PepT1 transgenic mice were infected ex vivo with C. rodentium for 6 h. ( A ) C. rodentium adherence to cultured colonic tissues. KC mRNA and protein levels were determined by real-time RT-PCR ( B ) and ELISA ( C ), respectively. Data are means ± S.E.M. * P

    Article Snippet: Consistent with these ex vivo data, colonization of C. rodentium in colons from PepT1 transgenic mice was reduced compared to those from WT mice ( ).

    Techniques: Over Expression, Cell Culture, Transgenic Assay, Mouse Assay, Infection, Ex Vivo, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Citrobacter rodentium induces PepT1 expression in mouse colon ( A, B ) Cultured mouse colonic tissues were infected with C. rodentium for 6 h and analyzed for PepT1 expression by real-time RT-PCR ( A ) and Western blot ( B ). ( C, D ) For infection of mice, food was withdrawn and drinking water was replaced with C. rodentium suspension in 20% sucrose distilled water overnight. Mice were euthanized 7 days after infection. PepT1 expression in mouse colon was analyzed by real-time RT-PCR ( C ) and Western blot ( D ). Data are means ± S.E.M. * P

    Journal: Gastroenterology

    Article Title: Pathogenic bacteria induce colonic PepT1 expression: an implication in host defense response

    doi: 10.1053/j.gastro.2009.06.043

    Figure Lengend Snippet: Citrobacter rodentium induces PepT1 expression in mouse colon ( A, B ) Cultured mouse colonic tissues were infected with C. rodentium for 6 h and analyzed for PepT1 expression by real-time RT-PCR ( A ) and Western blot ( B ). ( C, D ) For infection of mice, food was withdrawn and drinking water was replaced with C. rodentium suspension in 20% sucrose distilled water overnight. Mice were euthanized 7 days after infection. PepT1 expression in mouse colon was analyzed by real-time RT-PCR ( C ) and Western blot ( D ). Data are means ± S.E.M. * P

    Article Snippet: Consistent with these ex vivo data, colonization of C. rodentium in colons from PepT1 transgenic mice was reduced compared to those from WT mice ( ).

    Techniques: Expressing, Cell Culture, Infection, Quantitative RT-PCR, Western Blot, Mouse Assay

    NKCC1 is required for enteric C rodentium infection clearance. NKCC1 WT/WT , NKCC1 WT/DFX , and NKCC1 DFX/DFX mice were infected with 10 9 colony forming units of a kanamycin-resistant strain of C rodentium . Feces were collected every day, eluted, plated, and bacteria were enumerated. ( A ) Percentage of NKCC1 WT/WT , NKCC1 WT/DFX , and NKCC1 DFX /DFX mice with positive C rodentium shedding in feces over the 9-day period. ( B ) Bacteria plated on a kanamycin-containing plate show colonization or delay clearance in NKCC1 WT/DFX and NKCC1 DFX/DFX mice. ( C ) Inflammatory cytokine production in the serum of NKCC1 WT/WT , NKCC1 WT/DFX , and NKKCC1 DFX/DFX mice after 9 days postinfection (n = 3–5 mice per group, the experiment was repeated twice). hets, heterozygotes; homos, homozygotes; IL, interleukin; INF, interferon.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Novel Human NKCC1 Mutations Cause Defects in Goblet Cell Mucus Secretion and Chronic Inflammation

    doi: 10.1016/j.jcmgh.2019.10.006

    Figure Lengend Snippet: NKCC1 is required for enteric C rodentium infection clearance. NKCC1 WT/WT , NKCC1 WT/DFX , and NKCC1 DFX/DFX mice were infected with 10 9 colony forming units of a kanamycin-resistant strain of C rodentium . Feces were collected every day, eluted, plated, and bacteria were enumerated. ( A ) Percentage of NKCC1 WT/WT , NKCC1 WT/DFX , and NKCC1 DFX /DFX mice with positive C rodentium shedding in feces over the 9-day period. ( B ) Bacteria plated on a kanamycin-containing plate show colonization or delay clearance in NKCC1 WT/DFX and NKCC1 DFX/DFX mice. ( C ) Inflammatory cytokine production in the serum of NKCC1 WT/WT , NKCC1 WT/DFX , and NKKCC1 DFX/DFX mice after 9 days postinfection (n = 3–5 mice per group, the experiment was repeated twice). hets, heterozygotes; homos, homozygotes; IL, interleukin; INF, interferon.

    Article Snippet: C rodentium (ATCC 51459) were grown overnight at 37°C in Luria Broth medium.

    Techniques: Infection, Mouse Assay