citrobacter rodentium  (ATCC)


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    Structured Review

    ATCC citrobacter rodentium
    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with <t>Citrobacter</t> <t>rodentium</t> (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Citrobacter Rodentium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling"

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    Journal: FEMS immunology and medical microbiology

    doi: 10.1111/j.1574-695X.2012.00978.x

    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Figure Legend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Techniques Used: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium
    Figure Legend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Techniques Used: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination
    Figure Legend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Techniques Used: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)
    Figure Legend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Techniques Used: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed
    Figure Legend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Techniques Used: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)
    Figure Legend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Techniques Used: Activation Assay, Mouse Assay

    2) Product Images from "Infection with enteric pathogens Salmonella typhimurium and Citrobacter rodentium modulate TGF-beta/Smad signaling pathways in the intestine"

    Article Title: Infection with enteric pathogens Salmonella typhimurium and Citrobacter rodentium modulate TGF-beta/Smad signaling pathways in the intestine

    Journal: Gut Microbes

    doi: 10.1080/19490976.2018.1429878

    Smad 2/3 Immunostaining in response to Citrobacter infection. OCT embedded tissue sections of representative region of the colon were fixed and stained for total Smad2/3 (alexa fluor 488, green) and Villin (alexa fluor 568, red). A decrease in immunofluorescence staining of Smad2 and Smad3 proteins in the distal colon tissues is shown following C. rodentium infection in representative images. (scale bar: 50 μm).
    Figure Legend Snippet: Smad 2/3 Immunostaining in response to Citrobacter infection. OCT embedded tissue sections of representative region of the colon were fixed and stained for total Smad2/3 (alexa fluor 488, green) and Villin (alexa fluor 568, red). A decrease in immunofluorescence staining of Smad2 and Smad3 proteins in the distal colon tissues is shown following C. rodentium infection in representative images. (scale bar: 50 μm).

    Techniques Used: Immunostaining, Infection, Staining, Immunofluorescence

    Citrobacter rodentium infection decreases colonic mucosal TGF-R1 and TGF-R2 mRNA levels and downregulates Smad2 and Smad3 levels. Streptomycin-pretreated FVB/N mice were colonized with C. rodentium or control for 6 days. RNA extracted from mouse colonic mucosa was amplified using gene specific primers for real-time PCR quantification. Data represent the relative mRNA expression of TGF-R1 (A), TGF-RII (B), Smad2 (C) and Smad3 (D) normalized to the respective GAPDH mRNA (internal control) levels. Data are expressed as mean ± SEM. *p 0.05; **p
    Figure Legend Snippet: Citrobacter rodentium infection decreases colonic mucosal TGF-R1 and TGF-R2 mRNA levels and downregulates Smad2 and Smad3 levels. Streptomycin-pretreated FVB/N mice were colonized with C. rodentium or control for 6 days. RNA extracted from mouse colonic mucosa was amplified using gene specific primers for real-time PCR quantification. Data represent the relative mRNA expression of TGF-R1 (A), TGF-RII (B), Smad2 (C) and Smad3 (D) normalized to the respective GAPDH mRNA (internal control) levels. Data are expressed as mean ± SEM. *p 0.05; **p

    Techniques Used: Infection, Mouse Assay, Amplification, Real-time Polymerase Chain Reaction, Expressing

    3) Product Images from "The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection"

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection

    Journal: Nature immunology

    doi: 10.1038/ni.3118

    IRF1 is not required for canonical or non-canonical NLRP3 or NLRC4 inflammasome activation ( a ) Caspase-1 activation, ( b ) IL-1β and IL-18 release and cell death in LPS-primed BMDMs stimulated with ATP or nigericin or in unprimed BMDMs infected with Citrobacter rodentium (MOI 20) or ΔfliCΔfljB mutant S. Typhimurium (MOI 20) or wild-type S. Typhimurium ( S Tm) for 20 h. ( c ) Caspase-1 activation, ( d ) IL-1β and IL-18 release and cell death in unprimed BMDMs infected with log-phase grown S. Typhimurium (MOI 1) for 4 h. Graphs show mean and s.e.m. of three independent experiments. NS, not significant (two-tailed t -test).
    Figure Legend Snippet: IRF1 is not required for canonical or non-canonical NLRP3 or NLRC4 inflammasome activation ( a ) Caspase-1 activation, ( b ) IL-1β and IL-18 release and cell death in LPS-primed BMDMs stimulated with ATP or nigericin or in unprimed BMDMs infected with Citrobacter rodentium (MOI 20) or ΔfliCΔfljB mutant S. Typhimurium (MOI 20) or wild-type S. Typhimurium ( S Tm) for 20 h. ( c ) Caspase-1 activation, ( d ) IL-1β and IL-18 release and cell death in unprimed BMDMs infected with log-phase grown S. Typhimurium (MOI 1) for 4 h. Graphs show mean and s.e.m. of three independent experiments. NS, not significant (two-tailed t -test).

    Techniques Used: Activation Assay, Infection, Mutagenesis, Two Tailed Test

    4) Product Images from "Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection"

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004410

    Enhanced inflammasome activation in Nod2 −/− and Rip2 −/− BMDMs. BMDM were generated from WT, Nod2 −/− and Rip2 −/− mice and infected with 20MOI of Citrobacter rodentium for 18 h. (A–F) Combined supernatant and lysates were examined by Western blot for caspase-1 cleavage (casp-1p20) visually (A,D) and by densitometry (B,E), or (C,F) supernatants were examined for IL-18 secretion by ELISA. (G) BMDM were infected with C. rodentium and assayed for intracellular growth at the indicated times post-infection. (A–F) Data are representative of five independent experiments with n = 2–3 wells per experiment. (G) Data are representative of two independent experiments with n = 2–3 wells per experiment. (B,C,E,F,G) Data are shown as the mean ± SEM. (*, p
    Figure Legend Snippet: Enhanced inflammasome activation in Nod2 −/− and Rip2 −/− BMDMs. BMDM were generated from WT, Nod2 −/− and Rip2 −/− mice and infected with 20MOI of Citrobacter rodentium for 18 h. (A–F) Combined supernatant and lysates were examined by Western blot for caspase-1 cleavage (casp-1p20) visually (A,D) and by densitometry (B,E), or (C,F) supernatants were examined for IL-18 secretion by ELISA. (G) BMDM were infected with C. rodentium and assayed for intracellular growth at the indicated times post-infection. (A–F) Data are representative of five independent experiments with n = 2–3 wells per experiment. (G) Data are representative of two independent experiments with n = 2–3 wells per experiment. (B,C,E,F,G) Data are shown as the mean ± SEM. (*, p

    Techniques Used: Activation Assay, Generated, Mouse Assay, Infection, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    Transferring:

    Article Title: Host and microbiota factors that control Klebsiella pneumoniae mucosal colonization in mice
    Article Snippet: Citrobacter rodentium (ATCC 51459, Rockville, MD) was grown in LB media overnight at 37 °C. .. The syringe containing bacteria was mounted onto a Stepper repetitive pipette (Tridak, Brookfield, CT) to deliver an equal amount of inoculum (50 μL) to each mouse.

    Electrophoresis:

    Article Title: The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli ▿ ▿ † ▿ † ‡
    Article Snippet: The strain of C. rodentium sequenced, strain ICC168, was obtained in 1993 from S. W. Barthold's original stocks, previously known as Citrobacter freundii biotype 4280 (ATCC 51459) ( , ), and subjected to minimal passages. .. Ten μg genomic DNA was fragmented by multiple passages through a 30-gauge needle and end repaired with a mixture of Klenow polymerase, T4 DNA polymerase, and polynucleotide kinase before size selection by preparative electrophoresis through a 0.8% agarose gel.

    Nuclear Magnetic Resonance:

    Article Title: Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies
    Article Snippet: Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. The core oligosaccharide component from Mannheimia (Pasteurella) haemolytica serotype A1 lipopolysaccharide contains L -glycero -D- manno- and D- glycero -D- manno heptoses: Analysis of the structure and conformation by high-resolution NMR spectroscopy.

    Cell Culture:

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection
    Article Snippet: Paragraph title: Cell culture and stimulation ... S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (ΔfliC ΔfljB S Tm) and Citrobacter rodentium (ATCC 51459) were inoculated into LB broth and incubated overnight under aerobic conditions at 37°C.

    Article Title: Citrobacter Infection and Wnt signaling
    Article Snippet: Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. Ong SE, Mann MA. practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) Nature Protocols.

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling
    Article Snippet: Probiotic L. acidophilus (La) was cultured in deMan, Rogosa, and Sharpe broth (MRS; Difco, Detroit, MI) and grown at 37 °C for 20 h and re-suspended in PBS prior to oral inoculation (1 × 109 CFU per mouse). .. Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Expressing:

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection
    Article Snippet: S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (ΔfliC ΔfljB S Tm) and Citrobacter rodentium (ATCC 51459) were inoculated into LB broth and incubated overnight under aerobic conditions at 37°C. .. The following conditions were used: F. novicida or F. novicida ΔmglA (MOI 100 and 20 h for caspase-1 activation; MOI 50 for 2, 8, 16 and 24 h for IRF1 or GBP expression), S. Typhimurium (MOI 1, 4 h), ΔfliC ΔfljB S Tm (MOI 20 for 20 h) and C. rodentium (MOI 20 for 20 h).

    Knock-In:

    Article Title: Autophagy Controls an Intrinsic Host Defense to Bacteria by Promoting Epithelial Cell Survival: A Murine Model
    Article Snippet: LC3-GFP knock-in mice, ATG5 flox/flox mice, and ATG7 flox/flox mice were purchased from RIKEN BRC (Tsukuba-shi, Ibaraki, Japan). .. Virulent Shigella flexneri 5a (M90T), IpaB4 deletion mutant (M90T∆IpaB4), enteropathogenic Escherichia coli (EPEC) from Dr. Chihiro Sasakawa (University of Tokyo, Japan), and Citrobacter rodentium from ATCC (Rockville, MD) were used for infection.

    Western Blot:

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection
    Article Snippet: Citrobacter rodentium (ATCC 51459), Salmonella typhimurium (SL1344), Salmonella typhimurium Δfljb/flic were grown in LB broth overnight with shaking at 37°C. .. Cell lysates combined with supernatants were collected after 18 h of infection and assessed for caspase-1 activation by Western blot using the anti-caspase-1 p20 antibody from Adipogen (AG-20B-0042-C100).

    Derivative Assay:

    Article Title: Host and microbiota factors that control Klebsiella pneumoniae mucosal colonization in mice
    Article Snippet: K. pneumoniae strain IA565, described elsewhere [ ], and it’s streptomycin resistant derived strain, IA565S were grown in tryptic soy broth (Difco, Detroit, MI) and luria broth (LB) containing 50μg/mL streptomycin (Teknova, Hollister, CA), respectively, overnight at 37 °C. .. Citrobacter rodentium (ATCC 51459, Rockville, MD) was grown in LB media overnight at 37 °C.

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection
    Article Snippet: Bacterial infection, caspase-1 activation and IL-18 production WT, Nod2 −/− and Rip2 −/− bone marrow derived macrophages (BMDM) were differentiated in complete IMDM containing 10% heat-inactivated FBS and supplemented with M-CSF containing L929 supernatant at 37°C in a humidified atmosphere containing 5% CO2 for 5 days. .. Citrobacter rodentium (ATCC 51459), Salmonella typhimurium (SL1344), Salmonella typhimurium Δfljb/flic were grown in LB broth overnight with shaking at 37°C.

    Transfection:

    Article Title: NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component
    Article Snippet: Measurement of cytokine production Cells were seeded onto 96-well plates at 1×105 cells per well and stimulated as follows: LPS (10 ng/ml, 4 h); MALP-2 (400 pg/ml, 4 h); R848 (20 ng/ml, 4 h); nigericin (10 μg/ml, 1 h); ATP (5 mM, 1 h); alum (400 μg/ml, 8 h), Escherichia coli , (ATCC 11775, 16 h) and Citrobacter rodentium (ATCC 51459, 16 h). .. Poly(dA:dT) (4 μg/ml, 8 h) and flagellin (500 ng/ml, 2 h) were complexed with Lipofectamine 2000 (Life Technologies) and transfected according to the manufacturer's instructions.

    Activation Assay:

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection
    Article Snippet: S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (ΔfliC ΔfljB S Tm) and Citrobacter rodentium (ATCC 51459) were inoculated into LB broth and incubated overnight under aerobic conditions at 37°C. .. The following conditions were used: F. novicida or F. novicida ΔmglA (MOI 100 and 20 h for caspase-1 activation; MOI 50 for 2, 8, 16 and 24 h for IRF1 or GBP expression), S. Typhimurium (MOI 1, 4 h), ΔfliC ΔfljB S Tm (MOI 20 for 20 h) and C. rodentium (MOI 20 for 20 h).

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection
    Article Snippet: Paragraph title: Bacterial infection, caspase-1 activation and IL-18 production ... Citrobacter rodentium (ATCC 51459), Salmonella typhimurium (SL1344), Salmonella typhimurium Δfljb/flic were grown in LB broth overnight with shaking at 37°C.

    Low Copy Number:

    Article Title: The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli ▿ ▿ † ▿ † ‡
    Article Snippet: The strain of C. rodentium sequenced, strain ICC168, was obtained in 1993 from S. W. Barthold's original stocks, previously known as Citrobacter freundii biotype 4280 (ATCC 51459) ( , ), and subjected to minimal passages. .. Two- to 2.2-kb and 2.2- to 4-kb fractions were ligated into pUC19_smaI, and 4- to 5-, 5- to 6-, 6- to 9-, and 9- to 10-kb fractions were ligated into the low-copy-number vector pMAQ1b_smaI.

    Infection:

    Article Title: Citrobacter Infection and Wnt signaling
    Article Snippet: Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. Diez E, Zhu L, Teatero SA, Paquet M, Roy MF, Loredo-Osti JC, Malo D, Gruenheid S. Identification and characterization of Cri1, a locus controlling mortality during Citrobacter rodentium infection in mice.

    Article Title: Autophagy Controls an Intrinsic Host Defense to Bacteria by Promoting Epithelial Cell Survival: A Murine Model
    Article Snippet: .. Virulent Shigella flexneri 5a (M90T), IpaB4 deletion mutant (M90T∆IpaB4), enteropathogenic Escherichia coli (EPEC) from Dr. Chihiro Sasakawa (University of Tokyo, Japan), and Citrobacter rodentium from ATCC (Rockville, MD) were used for infection. ..

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection
    Article Snippet: Paragraph title: Bacterial infection, caspase-1 activation and IL-18 production ... Citrobacter rodentium (ATCC 51459), Salmonella typhimurium (SL1344), Salmonella typhimurium Δfljb/flic were grown in LB broth overnight with shaking at 37°C.

    Sequencing:

    Article Title: The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli ▿ ▿ † ▿ † ‡
    Article Snippet: Paragraph title: Bacterial strains and sequencing. ... The strain of C. rodentium sequenced, strain ICC168, was obtained in 1993 from S. W. Barthold's original stocks, previously known as Citrobacter freundii biotype 4280 (ATCC 51459) ( , ), and subjected to minimal passages.

    Article Title: Citrobacter rodentium is an Unstable Pathogen Showing Evidence of Significant Genomic Flux
    Article Snippet: .. We previously determined the whole genome sequence of C. rodentium strain ICC168, a derivative of a strain isolated from a disease outbreak in Swiss-Webster mice at Yale University School of Medicine, USA, in 1972, originally designated Citrobacter freundii biotype 4280 (ATCC 51459) , . ..

    Sonication:

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling
    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse). .. The bacterial culture was washed in PBS and sonicated on ice.

    Mutagenesis:

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection
    Article Snippet: .. S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (ΔfliC ΔfljB S Tm) and Citrobacter rodentium (ATCC 51459) were inoculated into LB broth and incubated overnight under aerobic conditions at 37°C. ..

    Article Title: Autophagy Controls an Intrinsic Host Defense to Bacteria by Promoting Epithelial Cell Survival: A Murine Model
    Article Snippet: .. Virulent Shigella flexneri 5a (M90T), IpaB4 deletion mutant (M90T∆IpaB4), enteropathogenic Escherichia coli (EPEC) from Dr. Chihiro Sasakawa (University of Tokyo, Japan), and Citrobacter rodentium from ATCC (Rockville, MD) were used for infection. ..

    Article Title: IRGB10 liberates bacterial ligands for sensing by the AIM2 and caspase-11–NLRP3 inflammasomes
    Article Snippet: .. S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (Δ fliC Δ fljB S Tm), Citrobacter rodentium (51459, American Type Culture Collection) and Escherichia coli (11775, American Type Culture Collection) were inoculated into Luria-Bertani media (3002-031, MP Biomedicals) and incubated overnight under aerobic conditions at 37°C. ..

    Isolation:

    Article Title: Citrobacter rodentium is an Unstable Pathogen Showing Evidence of Significant Genomic Flux
    Article Snippet: .. We previously determined the whole genome sequence of C. rodentium strain ICC168, a derivative of a strain isolated from a disease outbreak in Swiss-Webster mice at Yale University School of Medicine, USA, in 1972, originally designated Citrobacter freundii biotype 4280 (ATCC 51459) , . ..

    Article Title: The Prophages of Citrobacter rodentium Represent a Conserved Family of Horizontally Acquired Mobile Genetic Elements Associated with Enteric Evolution towards Pathogenicity
    Article Snippet: C. rodentium was first isolated in 1972 from an outbreak of diarrhea in Swiss-Webster mice and then “rediscovered” by Schauer and Falkow in 1993 ( ). .. It was originally classified as Citrobacter freundii biotype 4280 (ATCC 51459).

    Labeling:

    Article Title: Citrobacter Infection and Wnt signaling
    Article Snippet: Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. Ong SE, Mann MA. practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) Nature Protocols.

    Mouse Assay:

    Article Title: Citrobacter rodentium is an Unstable Pathogen Showing Evidence of Significant Genomic Flux
    Article Snippet: .. We previously determined the whole genome sequence of C. rodentium strain ICC168, a derivative of a strain isolated from a disease outbreak in Swiss-Webster mice at Yale University School of Medicine, USA, in 1972, originally designated Citrobacter freundii biotype 4280 (ATCC 51459) , . ..

    Article Title: Citrobacter Infection and Wnt signaling
    Article Snippet: Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. Diez E, Zhu L, Teatero SA, Paquet M, Roy MF, Loredo-Osti JC, Malo D, Gruenheid S. Identification and characterization of Cri1, a locus controlling mortality during Citrobacter rodentium infection in mice.

    Article Title: The Prophages of Citrobacter rodentium Represent a Conserved Family of Horizontally Acquired Mobile Genetic Elements Associated with Enteric Evolution towards Pathogenicity
    Article Snippet: C. rodentium was first isolated in 1972 from an outbreak of diarrhea in Swiss-Webster mice and then “rediscovered” by Schauer and Falkow in 1993 ( ). .. It was originally classified as Citrobacter freundii biotype 4280 (ATCC 51459).

    Article Title: Autophagy Controls an Intrinsic Host Defense to Bacteria by Promoting Epithelial Cell Survival: A Murine Model
    Article Snippet: Paragraph title: Mice and Bacteria strains ... Virulent Shigella flexneri 5a (M90T), IpaB4 deletion mutant (M90T∆IpaB4), enteropathogenic Escherichia coli (EPEC) from Dr. Chihiro Sasakawa (University of Tokyo, Japan), and Citrobacter rodentium from ATCC (Rockville, MD) were used for infection.

    Article Title: Host and microbiota factors that control Klebsiella pneumoniae mucosal colonization in mice
    Article Snippet: Citrobacter rodentium (ATCC 51459, Rockville, MD) was grown in LB media overnight at 37 °C. .. For intranasal inoculation, mice were anesthetized with ketamine and xylazine and a 5–10 μL inoculum of bacteria was given into each nostril followed by a 5 μL flush with sterile saline in each nostril.

    Spectroscopy:

    Article Title: Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies
    Article Snippet: Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. The core oligosaccharide component from Mannheimia (Pasteurella) haemolytica serotype A1 lipopolysaccharide contains L -glycero -D- manno- and D- glycero -D- manno heptoses: Analysis of the structure and conformation by high-resolution NMR spectroscopy.

    Silver Staining:

    Article Title: Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies
    Article Snippet: A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. .. Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem.

    Plasmid Preparation:

    Article Title: The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli ▿ ▿ † ▿ † ‡
    Article Snippet: The strain of C. rodentium sequenced, strain ICC168, was obtained in 1993 from S. W. Barthold's original stocks, previously known as Citrobacter freundii biotype 4280 (ATCC 51459) ( , ), and subjected to minimal passages. .. Two- to 2.2-kb and 2.2- to 4-kb fractions were ligated into pUC19_smaI, and 4- to 5-, 5- to 6-, 6- to 9-, and 9- to 10-kb fractions were ligated into the low-copy-number vector pMAQ1b_smaI.

    Software:

    Article Title: Reactive Oxygen Species Regulate Caspase-11 Expression and Activation of the Non-canonical NLRP3 Inflammasome during Enteric Pathogen Infection
    Article Snippet: Citrobacter rodentium (ATCC 51459), Salmonella typhimurium (SL1344), Salmonella typhimurium Δfljb/flic were grown in LB broth overnight with shaking at 37°C. .. Western blots were imaged on a Kodak IMM4000pro and the densitometry performed using the Carestream software provided by the manufacturer.

    Selection:

    Article Title: The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli ▿ ▿ † ▿ † ‡
    Article Snippet: The strain of C. rodentium sequenced, strain ICC168, was obtained in 1993 from S. W. Barthold's original stocks, previously known as Citrobacter freundii biotype 4280 (ATCC 51459) ( , ), and subjected to minimal passages. .. Ten μg genomic DNA was fragmented by multiple passages through a 30-gauge needle and end repaired with a mixture of Klenow polymerase, T4 DNA polymerase, and polynucleotide kinase before size selection by preparative electrophoresis through a 0.8% agarose gel.

    Agarose Gel Electrophoresis:

    Article Title: The Citrobacter rodentium Genome Sequence Reveals Convergent Evolution with Human Pathogenic Escherichia coli ▿ ▿ † ▿ † ‡
    Article Snippet: The strain of C. rodentium sequenced, strain ICC168, was obtained in 1993 from S. W. Barthold's original stocks, previously known as Citrobacter freundii biotype 4280 (ATCC 51459) ( , ), and subjected to minimal passages. .. Ten μg genomic DNA was fragmented by multiple passages through a 30-gauge needle and end repaired with a mixture of Klenow polymerase, T4 DNA polymerase, and polynucleotide kinase before size selection by preparative electrophoresis through a 0.8% agarose gel.

    Homogenization:

    Article Title: NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component
    Article Snippet: Measurement of cytokine production Cells were seeded onto 96-well plates at 1×105 cells per well and stimulated as follows: LPS (10 ng/ml, 4 h); MALP-2 (400 pg/ml, 4 h); R848 (20 ng/ml, 4 h); nigericin (10 μg/ml, 1 h); ATP (5 mM, 1 h); alum (400 μg/ml, 8 h), Escherichia coli , (ATCC 11775, 16 h) and Citrobacter rodentium (ATCC 51459, 16 h). .. HEK293T cells were washed with cold PBS and lysed in Homogenization buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2 , 250 mM sucrose) by 20 strokes in a Dounce homogenizer.

    Incubation:

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection
    Article Snippet: .. S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (ΔfliC ΔfljB S Tm) and Citrobacter rodentium (ATCC 51459) were inoculated into LB broth and incubated overnight under aerobic conditions at 37°C. ..

    Article Title: Differential roles of caspase-1 and caspase-11 in infection and inflammation
    Article Snippet: .. Microbial culture S. Typhimurium SL1344, Citrobacter rodentium (51459, American Type Culture Collection) and Escherichia coli (11775, American Type Culture Collection) were inoculated into Luria-Bertani media (3002–031, MP Biomedicals) and incubated under aerobic conditions overnight at 37 °C. ..

    Article Title: Infection with enteric pathogens Salmonella typhimurium and Citrobacter rodentium modulate TGF-beta/Smad signaling pathways in the intestine
    Article Snippet: Bacterial strains used in this study were wild-type Salmonella ATCC 14028 and Citrobacter rodentium (ATCC , DBS100/255). .. Non-agitated microaerophilic Salmonella cultures were prepared by inoculating 10 ml of Luria-Bertani broth with 0.01 ml of a stationary phase culture and were incubated overnight (∼18 h) at 37°C, as previously described.

    Article Title: IRGB10 liberates bacterial ligands for sensing by the AIM2 and caspase-11–NLRP3 inflammasomes
    Article Snippet: .. S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (Δ fliC Δ fljB S Tm), Citrobacter rodentium (51459, American Type Culture Collection) and Escherichia coli (11775, American Type Culture Collection) were inoculated into Luria-Bertani media (3002-031, MP Biomedicals) and incubated overnight under aerobic conditions at 37°C. ..

    Produced:

    Article Title: Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies
    Article Snippet: .. Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. ..

    Article Title: Citrobacter Infection and Wnt signaling
    Article Snippet: .. Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459) Eur J Biochem. .. Diez E, Zhu L, Teatero SA, Paquet M, Roy MF, Loredo-Osti JC, Malo D, Gruenheid S. Identification and characterization of Cri1, a locus controlling mortality during Citrobacter rodentium infection in mice.

    Article Title: Toll-Like Receptor 4 Contributes to Colitis Development but Not to Host Defense during Citrobacter rodentium Infection in Mice
    Article Snippet: .. Structural studies on the O-polysaccharide of the lipopolysaccharide produced by Citrobacter rodentium (ATCC 51459). ..

    Concentration Assay:

    Article Title: The transcription factor IRF1 and guanylate-binding proteins target AIM2 inflammasome activation by Francisella infection
    Article Snippet: BMDMs were seeded in antibiotic–free media at a concentration of 1 × 106 cells onto 12-well plates and incubated overnight. .. S. Typhimurium SL1344, an isogenic mutant lacking fliC and fljB (ΔfliC ΔfljB S Tm) and Citrobacter rodentium (ATCC 51459) were inoculated into LB broth and incubated overnight under aerobic conditions at 37°C.

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  • 86
    ATCC c rodentium infection
    IECs serve as gatekeeper for the supply of AHR ligands to the host. a, Cyp1a1 expression gated on live colonic cells of mice fed control or I3C containing diet for two weeks (left panel) and colon sections stained for E-Cadherin (red), Cyp1a1 (green) and DAPI (blue). Scale bars represent 100μm (right panel). b , Cyp1a1 expression in the colon of WT Cyp1a1 reporter (WT) and Cyp1 -/- Cyp1a1 reporter mice treated with control or I3C diet. c , Absolute numbers of RORγt + ILC3 in mice of indicated genotypes, mean ± s.e.m. ( n =3 per group). d , Survival plot of C. <t>rodentium</t> infected mice ( n =4 per group). e , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue) of infected mice (day 7). Scale bars represent 100μm. f , Representative photomicrographs of (H E) stained colon sections of C. rodentium infected mice (day 7). Scale bars represent 100μm. g , C. rodentium burdens. Bars show the median, symbols represent individual mice. h , Absolute numbers of colonic RORγt + ILC3 and Th17 cells (WT: n =6, IEC Cyp1a1 : n =3) and IL-22 protein in colon explant cultures ( n =6 per group), mean ± s.e.m.. i , Pathology scores (day 6) of distal colon and caecum in bone marrow chimeras of wildtype (WT→WT) or Cyp1 deficient (WT→ Cyp1 -/- ) recipients. Bars show the mean, symbols represent individual mice. j , C. rodentium burdens in the colon. Bars are the median and symbols represent individual mice. k , Absolute numbers of WT donor-derived colonic RORγt + ILC3 and Th17 cells ( n =7 per group) and IL-22 protein in distal colon organ explant cultures (WT: n =7, Cyp1 -/- : n =6), mean ± s.e.m.. Results are representative of three independent experiments. * P
    C Rodentium Infection, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c rodentium
    Deficiency in inflammasome components increases host susceptibility to C. <t>rodentium</t> infection. WT, Nlrp3 −/− , and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. A , body weight
    C Rodentium, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC citrobacter rodentium
    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with <t>Citrobacter</t> <t>rodentium</t> (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
    Citrobacter Rodentium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC citrobacter rodentium colonies
    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with <t>Citrobacter</t> <t>rodentium</t> (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and
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    IECs serve as gatekeeper for the supply of AHR ligands to the host. a, Cyp1a1 expression gated on live colonic cells of mice fed control or I3C containing diet for two weeks (left panel) and colon sections stained for E-Cadherin (red), Cyp1a1 (green) and DAPI (blue). Scale bars represent 100μm (right panel). b , Cyp1a1 expression in the colon of WT Cyp1a1 reporter (WT) and Cyp1 -/- Cyp1a1 reporter mice treated with control or I3C diet. c , Absolute numbers of RORγt + ILC3 in mice of indicated genotypes, mean ± s.e.m. ( n =3 per group). d , Survival plot of C. rodentium infected mice ( n =4 per group). e , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue) of infected mice (day 7). Scale bars represent 100μm. f , Representative photomicrographs of (H E) stained colon sections of C. rodentium infected mice (day 7). Scale bars represent 100μm. g , C. rodentium burdens. Bars show the median, symbols represent individual mice. h , Absolute numbers of colonic RORγt + ILC3 and Th17 cells (WT: n =6, IEC Cyp1a1 : n =3) and IL-22 protein in colon explant cultures ( n =6 per group), mean ± s.e.m.. i , Pathology scores (day 6) of distal colon and caecum in bone marrow chimeras of wildtype (WT→WT) or Cyp1 deficient (WT→ Cyp1 -/- ) recipients. Bars show the mean, symbols represent individual mice. j , C. rodentium burdens in the colon. Bars are the median and symbols represent individual mice. k , Absolute numbers of WT donor-derived colonic RORγt + ILC3 and Th17 cells ( n =7 per group) and IL-22 protein in distal colon organ explant cultures (WT: n =7, Cyp1 -/- : n =6), mean ± s.e.m.. Results are representative of three independent experiments. * P

    Journal: Nature

    Article Title: Feedback Control of AHR Signaling Regulates Intestinal Immunity

    doi: 10.1038/nature21080

    Figure Lengend Snippet: IECs serve as gatekeeper for the supply of AHR ligands to the host. a, Cyp1a1 expression gated on live colonic cells of mice fed control or I3C containing diet for two weeks (left panel) and colon sections stained for E-Cadherin (red), Cyp1a1 (green) and DAPI (blue). Scale bars represent 100μm (right panel). b , Cyp1a1 expression in the colon of WT Cyp1a1 reporter (WT) and Cyp1 -/- Cyp1a1 reporter mice treated with control or I3C diet. c , Absolute numbers of RORγt + ILC3 in mice of indicated genotypes, mean ± s.e.m. ( n =3 per group). d , Survival plot of C. rodentium infected mice ( n =4 per group). e , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue) of infected mice (day 7). Scale bars represent 100μm. f , Representative photomicrographs of (H E) stained colon sections of C. rodentium infected mice (day 7). Scale bars represent 100μm. g , C. rodentium burdens. Bars show the median, symbols represent individual mice. h , Absolute numbers of colonic RORγt + ILC3 and Th17 cells (WT: n =6, IEC Cyp1a1 : n =3) and IL-22 protein in colon explant cultures ( n =6 per group), mean ± s.e.m.. i , Pathology scores (day 6) of distal colon and caecum in bone marrow chimeras of wildtype (WT→WT) or Cyp1 deficient (WT→ Cyp1 -/- ) recipients. Bars show the mean, symbols represent individual mice. j , C. rodentium burdens in the colon. Bars are the median and symbols represent individual mice. k , Absolute numbers of WT donor-derived colonic RORγt + ILC3 and Th17 cells ( n =7 per group) and IL-22 protein in distal colon organ explant cultures (WT: n =7, Cyp1 -/- : n =6), mean ± s.e.m.. Results are representative of three independent experiments. * P

    Article Snippet: Infection with Citrobacter rodentium For C. rodentium infection a single colony of strain DBS100 (ATCC 51459; American Type Culture Collection) was transferred to Luria–Bertani (LB) broth and grown to log phase followed by centrifugation and resuspension in PBS.

    Techniques: Expressing, Mouse Assay, Staining, Infection, Derivative Assay

    Depletion of natural AHR ligands leads to impaired immunity to C. rodentium . a , Representative photomicrographs of H E stained colon sections of C. rodentium infected mice (day7). Scal e bars represent 200μm. b , Pathology scores. Bars show the mean and each symbol represents an individual mouse. c , Survival plot (WT: n =8, R26 Cyp1a1 : n =8, Ahr -/- : n =6). d , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. e , C. rodentium burdens in colon, liver and spleen. Bars show the median and each symbol represents an individual mouse. f , Absolute numbers of colonic RORγt + ILC3 (WT: n =7, R26 Cyp1a1 : n =7, Ahr -/- : n =3) and IL-17A-producing TCRβ + CD4 + T cells (WT: n =5, R26 Cyp1a1 : n =6, Ahr -/- : n =4), mean ± s.e.m. g , IL-22 protein colon explant cultures (WT: n =10, R26 Cyp1a1 : n =6, Ahr -/- : n =5), mean ± s.e.m. Data represent pooled results of at least two independent experiments. * P

    Journal: Nature

    Article Title: Feedback Control of AHR Signaling Regulates Intestinal Immunity

    doi: 10.1038/nature21080

    Figure Lengend Snippet: Depletion of natural AHR ligands leads to impaired immunity to C. rodentium . a , Representative photomicrographs of H E stained colon sections of C. rodentium infected mice (day7). Scal e bars represent 200μm. b , Pathology scores. Bars show the mean and each symbol represents an individual mouse. c , Survival plot (WT: n =8, R26 Cyp1a1 : n =8, Ahr -/- : n =6). d , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. e , C. rodentium burdens in colon, liver and spleen. Bars show the median and each symbol represents an individual mouse. f , Absolute numbers of colonic RORγt + ILC3 (WT: n =7, R26 Cyp1a1 : n =7, Ahr -/- : n =3) and IL-17A-producing TCRβ + CD4 + T cells (WT: n =5, R26 Cyp1a1 : n =6, Ahr -/- : n =4), mean ± s.e.m. g , IL-22 protein colon explant cultures (WT: n =10, R26 Cyp1a1 : n =6, Ahr -/- : n =5), mean ± s.e.m. Data represent pooled results of at least two independent experiments. * P

    Article Snippet: Infection with Citrobacter rodentium For C. rodentium infection a single colony of strain DBS100 (ATCC 51459; American Type Culture Collection) was transferred to Luria–Bertani (LB) broth and grown to log phase followed by centrifugation and resuspension in PBS.

    Techniques: Staining, Infection, Mouse Assay

    Dietary AHR ligands restore immunity to C. rodentium . a , Survival plot C. rodentium infected R26 Cyp1a1 mice fed control or I3C diet ( n =6 per group). b , C. rodentium burdens in faeces (Ctrl: n =5, I3C: n =6). c , Colon sections of C. rodentium infected mice stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. d , Representative photomicrographs of H E stained colon sections. Scale bars represent 100μm. e , Pathology scores. Bars show the mean and symbols represent individual mice. f , Absolute numbers of cytokine-producing TCRβ + CD4 + T cells in the colon of C. rodentium infected mice ( n =4 per group), mean ± s.e.m.. g , IL-17A and IL-22 expression in TCRβ + CD4 + T cells from the colon of C. rodentium infected mice. h , Survival plot of R26 Cyp1a1 mice fed I3C diet treated with anti-IL-22 blocking antibody ( n =6) or isotype control ( n =5). i , Concentration-dependent inhibition of human recombinant CYP1A1 enzyme activity by I3C, DIM and FICZ. Results are representative of three independent experiments. Error bars, mean ± s.e.m. * P

    Journal: Nature

    Article Title: Feedback Control of AHR Signaling Regulates Intestinal Immunity

    doi: 10.1038/nature21080

    Figure Lengend Snippet: Dietary AHR ligands restore immunity to C. rodentium . a , Survival plot C. rodentium infected R26 Cyp1a1 mice fed control or I3C diet ( n =6 per group). b , C. rodentium burdens in faeces (Ctrl: n =5, I3C: n =6). c , Colon sections of C. rodentium infected mice stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. d , Representative photomicrographs of H E stained colon sections. Scale bars represent 100μm. e , Pathology scores. Bars show the mean and symbols represent individual mice. f , Absolute numbers of cytokine-producing TCRβ + CD4 + T cells in the colon of C. rodentium infected mice ( n =4 per group), mean ± s.e.m.. g , IL-17A and IL-22 expression in TCRβ + CD4 + T cells from the colon of C. rodentium infected mice. h , Survival plot of R26 Cyp1a1 mice fed I3C diet treated with anti-IL-22 blocking antibody ( n =6) or isotype control ( n =5). i , Concentration-dependent inhibition of human recombinant CYP1A1 enzyme activity by I3C, DIM and FICZ. Results are representative of three independent experiments. Error bars, mean ± s.e.m. * P

    Article Snippet: Infection with Citrobacter rodentium For C. rodentium infection a single colony of strain DBS100 (ATCC 51459; American Type Culture Collection) was transferred to Luria–Bertani (LB) broth and grown to log phase followed by centrifugation and resuspension in PBS.

    Techniques: Infection, Mouse Assay, Staining, Expressing, Blocking Assay, Concentration Assay, Inhibition, Recombinant, Activity Assay

    Effects of Rag1-Cre mediated Cyp1a1 expression on immunity to C. rodentium . Mice of indicated genotypes were infected orally with ~2x10 9 C. rodentium and killed 14 days after infection or monitored for survival. a , Survival plot. b , C. rodentium burdens in the colon, cecum, liver and spleen. Bars are the median and each symbol represents an individual mouse. c , Pathology scores of distal colon and cecum. Bars are the mean and each symbol represents an individual mouse. d , Absolute numbers of cytokine-producing TCRβ + CD4 + T cells in the colon of C. rodentium infected mice. Results are representative of two independent experiments ( n =5 per group). NS = not significant, ** P

    Journal: Nature

    Article Title: Feedback Control of AHR Signaling Regulates Intestinal Immunity

    doi: 10.1038/nature21080

    Figure Lengend Snippet: Effects of Rag1-Cre mediated Cyp1a1 expression on immunity to C. rodentium . Mice of indicated genotypes were infected orally with ~2x10 9 C. rodentium and killed 14 days after infection or monitored for survival. a , Survival plot. b , C. rodentium burdens in the colon, cecum, liver and spleen. Bars are the median and each symbol represents an individual mouse. c , Pathology scores of distal colon and cecum. Bars are the mean and each symbol represents an individual mouse. d , Absolute numbers of cytokine-producing TCRβ + CD4 + T cells in the colon of C. rodentium infected mice. Results are representative of two independent experiments ( n =5 per group). NS = not significant, ** P

    Article Snippet: Infection with Citrobacter rodentium For C. rodentium infection a single colony of strain DBS100 (ATCC 51459; American Type Culture Collection) was transferred to Luria–Bertani (LB) broth and grown to log phase followed by centrifugation and resuspension in PBS.

    Techniques: Expressing, Mouse Assay, Infection

    IL-22-Fc improves resistance to C. rodentium infection in R26 Cyp1a1 mice. Mice of indicated genotypes were infected orally with ~2x10 9 C. rodentium and killed 7 days after infection or monitored for survival. a , C. rodentium burdens in the colon and caecum. Bars are the median and each symbol represents an individual mouse. b , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. c , Survival plot (WT: n =4, R26 Cyp1a1 +Ctrl Ig: n =6, R26 Cyp1a1 +IL-22Fc: n =5). Results are representative of three independent experiments. NS = not significant, ** P

    Journal: Nature

    Article Title: Feedback Control of AHR Signaling Regulates Intestinal Immunity

    doi: 10.1038/nature21080

    Figure Lengend Snippet: IL-22-Fc improves resistance to C. rodentium infection in R26 Cyp1a1 mice. Mice of indicated genotypes were infected orally with ~2x10 9 C. rodentium and killed 7 days after infection or monitored for survival. a , C. rodentium burdens in the colon and caecum. Bars are the median and each symbol represents an individual mouse. b , Colon sections stained for E-Cadherin (green), C. rodentium (red) and DAPI (blue). Scale bars represent 100μm. c , Survival plot (WT: n =4, R26 Cyp1a1 +Ctrl Ig: n =6, R26 Cyp1a1 +IL-22Fc: n =5). Results are representative of three independent experiments. NS = not significant, ** P

    Article Snippet: Infection with Citrobacter rodentium For C. rodentium infection a single colony of strain DBS100 (ATCC 51459; American Type Culture Collection) was transferred to Luria–Bertani (LB) broth and grown to log phase followed by centrifugation and resuspension in PBS.

    Techniques: Infection, Mouse Assay, Staining

    Deficiency in inflammasome components increases host susceptibility to C. rodentium infection. WT, Nlrp3 −/− , and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. A , body weight

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: Deficiency in inflammasome components increases host susceptibility to C. rodentium infection. WT, Nlrp3 −/− , and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. A , body weight

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Infection, Mouse Assay

    Increased adaptive immune responses in caspase-1-deficient mice infected with C. rodentium . A–C , WT and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. Mice were killed at days 0 (WT,

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: Increased adaptive immune responses in caspase-1-deficient mice infected with C. rodentium . A–C , WT and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. Mice were killed at days 0 (WT,

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Mouse Assay, Infection

    IL-1β and IL-18 production in the downstream of inflammasomes is critical for controlling C. rodentium infection. A and B , WT, Nlrp3 −/− , and Casp1 −/− mice were infected with C. rodentium for 14 days. Proteins were

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: IL-1β and IL-18 production in the downstream of inflammasomes is critical for controlling C. rodentium infection. A and B , WT, Nlrp3 −/− , and Casp1 −/− mice were infected with C. rodentium for 14 days. Proteins were

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Infection, Mouse Assay

    Defects in inflammasome activation are associated with increased inflammatory responses in the colon. WT and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. The infected mice were killed at

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: Defects in inflammasome activation are associated with increased inflammatory responses in the colon. WT and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10 9 cfu/mouse. The infected mice were killed at

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Activation Assay, Mouse Assay, Infection

    C. rodentium type III secretion system is dispensable for inflammasome activation in infected macrophages. BMDMs from WT and Casp1 −/− mice were primed with or without LPS for 5 h and then infected with C. rodentium, Δ escC (Mutant

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: C. rodentium type III secretion system is dispensable for inflammasome activation in infected macrophages. BMDMs from WT and Casp1 −/− mice were primed with or without LPS for 5 h and then infected with C. rodentium, Δ escC (Mutant

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Activation Assay, Infection, Mouse Assay, Mutagenesis

    C. rodentium induces caspase-1 activation through Nlrp3 inflammasome in cultured macrophages. Bone marrow-derived macrophages from WT, Casp1 −/− , Nlrp3 −/− , Nlrc4 −/− , and Aim2 −/− mice were

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: C. rodentium induces caspase-1 activation through Nlrp3 inflammasome in cultured macrophages. Bone marrow-derived macrophages from WT, Casp1 −/− , Nlrp3 −/− , Nlrc4 −/− , and Aim2 −/− mice were

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Activation Assay, Cell Culture, Derivative Assay, Mouse Assay

    Increased inflammatory cell infiltration correlates with high colonic bacterial burdens in inflammasome-deficient mice after C. rodentium infection. WT and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10

    Journal: The Journal of Biological Chemistry

    Article Title: Role of Inflammasomes in Host Defense against Citrobacter rodentium Infection *

    doi: 10.1074/jbc.M112.358705

    Figure Lengend Snippet: Increased inflammatory cell infiltration correlates with high colonic bacterial burdens in inflammasome-deficient mice after C. rodentium infection. WT and Casp1 −/− mice were orally infected with C. rodentium at a dose of 4 × 10

    Article Snippet: C. rodentium (ATCC 51459) was grown in LB broth overnight with shaking at 37 °C.

    Techniques: Mouse Assay, Infection

    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Techniques: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Techniques: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Techniques: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Techniques: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Techniques: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Article Snippet: Citrobacter rodentium (strain DBS100; American Type Culture Collection number 51459) was grown overnight in Luria broth (LB) and subsequently re-suspended in PBS prior to dosing (0.5 mL per mouse; approximately 5 × 108 CFU of C. rodentium per mouse).

    Techniques: Activation Assay, Mouse Assay

    Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Activation of intracellular NF-kB and Smad 7 in mouse epithelial cells. (a) Confluent CMT93 (p23) cells were incubated with Citrobacter rodentium (Cr, 2.5 × 10 7 CFU per well) in a six-well plate for 1 h. Cells were subsequently washed in PBS and

    Article Snippet: Citrobacter rodentium colonies were easily distinguished by appearance, using MacConkey agar–plated bacterial cultures from the manufacturer (strain DBS100; American Type Culture Collection number 51459) as a positive control.

    Techniques: Activation Assay, Incubation

    Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Experimental timeline. Three in vivo experiments were conducted in which 3-day-old mice along with lactating dams were randomly assigned to treatment groups of 7–10 mice pups per treatment. Group A (nontreated controls), group B ( Citrobacter rodentium

    Article Snippet: Citrobacter rodentium colonies were easily distinguished by appearance, using MacConkey agar–plated bacterial cultures from the manufacturer (strain DBS100; American Type Culture Collection number 51459) as a positive control.

    Techniques: In Vivo, Mouse Assay

    Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Early administration of probiotic Lactobacillus acidophilus (La) and/or prebiotic inulin reduces Citrobacter rodentium (Cr)-induced morbidity in mice. (a) Body weight changes of noninfected control mice (○) and mice pretreated with a combination

    Article Snippet: Citrobacter rodentium colonies were easily distinguished by appearance, using MacConkey agar–plated bacterial cultures from the manufacturer (strain DBS100; American Type Culture Collection number 51459) as a positive control.

    Techniques: Mouse Assay

    Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Representative histopathology of colonic tissues at 2 weeks post-Cr infection. Three independent experiments showing similar results with ≈ 7–10 mice per treatment. (a and d) Mice that were infected with Citrobacter rodentium only. (b)

    Article Snippet: Citrobacter rodentium colonies were easily distinguished by appearance, using MacConkey agar–plated bacterial cultures from the manufacturer (strain DBS100; American Type Culture Collection number 51459) as a positive control.

    Techniques: Histopathology, Infection, Mouse Assay

    Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Pretreatment with probiotic Lactobacillus acidophilus (La), prebiotic inulin, or synbiotics alters cytokine responses in the MLN of mice. Cells from the MLN were isolated from mice 2 weeks post- Citrobacter rodentium infection of each treatment and exposed

    Article Snippet: Citrobacter rodentium colonies were easily distinguished by appearance, using MacConkey agar–plated bacterial cultures from the manufacturer (strain DBS100; American Type Culture Collection number 51459) as a positive control.

    Techniques: Mouse Assay, Isolation, Infection

    Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Journal: FEMS immunology and medical microbiology

    Article Title: Early administration of probiotic Lactobacillus acidophilus and/or prebiotic inulin attenuates pathogen-mediated intestinal inflammation and Smad 7 cell signaling

    doi: 10.1111/j.1574-695X.2012.00978.x

    Figure Lengend Snippet: Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic)

    Article Snippet: Citrobacter rodentium colonies were easily distinguished by appearance, using MacConkey agar–plated bacterial cultures from the manufacturer (strain DBS100; American Type Culture Collection number 51459) as a positive control.

    Techniques: Activation Assay, Mouse Assay