ciprofloxacin cip  (Shanghai Aladdin Bio-Chem)

Journal: bioRxiv

Article Title: A multiplexed, target-based phenotypic screening platform using CRISPR interference in Mycobacterium abscessus

doi: 10.1101/2025.03.17.643728

Figure Lengend Snippet: A. Strain growth rates measured in single-plex. Growth (%) was calculated based on OD 600 values in liquid medium normalized to growth under uninduced CRISPRi conditions after 4 days (blue). Growth defects were seen in a majority of hypomorphs when CRISPRi was induced with 100 ng/mL anhydrotetracycline (AHT) (red). Ciprofloxacin (CIP) was used as a positive well-killing control (purple). Data are mean values and standard deviations from four technical replicates. B. Growth assay in multiplex. Strain abundance was measured using read counts as a surrogate after normalization to account for any variation in DNA extraction and PCR amplification between and within plates. CIP was used as a positive well-killing control (red) while DMSO was used as a negative control (blue). Median normalized read counts were obtained from twelve wells per plate. Data represents the mean values and SEM from seventy-two technical replicate plates.

Article Snippet: 1% DMSO (Sigma-Aldrich) was used as the negative untreated control and 25 μg/mL ciprofloxacin hydrochloride (CIP) (MP Biomedicals) was used as the positive control.

Techniques: Control, Growth Assay, Multiplex Assay, DNA Extraction, Amplification, Negative Control

Journal: Microbiology Spectrum

Article Title: Effectiveness of clindamycin-based exposure strategies in experimental mature staphylococcal biofilms

doi: 10.1128/spectrum.01947-24

Figure Lengend Snippet: Experimental approach of the different anti-biofilm assays, with each number indicating a step in the procedure. The different treatment strategies are shown in step 3. Antibiotic concentration ranges (mg/L) are represented by color gradients and applied in twofold dilution series. CIP: ciprofloxacin; CLI: clindamycin; RIF: rifampicin; TAV: Ti-6Al-4V implant material discs (created with BioRender.com).

Article Snippet: RIF (R3501, Sigma-Aldrich, 822.94 g/mol) was diluted in DMSO (4 g/L) and ciprofloxacin (CIP) (PHR 1044, Sigma-Aldrich, 385.82 g/mol) in Milli-Q water (25.6 g/L), before storage at −20°C until use.

Techniques: Concentration Assay

Journal: Microbiology Spectrum

Article Title: Effectiveness of clindamycin-based exposure strategies in experimental mature staphylococcal biofilms

doi: 10.1128/spectrum.01947-24

Figure Lengend Snippet: Visualization of mature 7-day biofilm-residing S. aureus LUH15392 on glass slides using confocal laser scanning microscopy (CLSM) (top) and on Ti-6Al-4V implant material discs using atomic force microscopy (AFM) (bottom). Images are divided by treatment: control biofilms are indicated by the - symbol; T1 indicates the first 24-h exposure, and T2 indicates the second 24-h exposure. Biofilm-embedded bacteria were visualized by LIVE (green)/DEAD(red) staining for CLSM. Apical biofilm is depicted on the bottom; basal biofilm is depicted on top of the z-stack. Scale bars indicate 20 µm. For the AFM, detailed 5 × 5 µm images were made at random locations on the biofilms. CIP: ciprofloxacin; CLI: clindamycin; MBC: minimum bactericidal concentration; RIF: rifampicin.

Article Snippet: RIF (R3501, Sigma-Aldrich, 822.94 g/mol) was diluted in DMSO (4 g/L) and ciprofloxacin (CIP) (PHR 1044, Sigma-Aldrich, 385.82 g/mol) in Milli-Q water (25.6 g/L), before storage at −20°C until use.

Techniques: Confocal Laser Scanning Microscopy, Microscopy, Control, Bacteria, Staining, Concentration Assay