Structured Review

PerkinElmer ci mmol
Ci Mmol, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 10 article reviews
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ci mmol - by Bioz Stars, 2020-04
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Related Articles

Transfection:

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: .. In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The affinities of drugs at the [3 H]WIN35,428 binding site on the DAT were determined by incubating tissue with a fixed concentration of [3 H]WIN35,428 and a range of drug concentrations.

Radioactivity:

Article Title: Diastereoselective Synthesis of Potent Antimalarial Cis-β-lactam Agents
Article Snippet: Then, 100 µL from a culture containing > 95% ring (0-20 h postinvasion) at a 0.8% parasitemia and 3% haematocrit in complete medium was added per well along with 1.0 µCi of 3H-hypoxanthine with a specific activity of 14.1 Ci/mmol (Perkin-Elmer, Courtaboeuf, France). .. Percentage growth inhibition was calculated from the parasite-associated radioactivity.

Filtration:

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The 1 h incubation at 22 °C was initiated by addition of membranes and terminated by rapid filtration over Whatman GF/B glass fiber filters pre-soaked in 0.1% polyethyleneimine (Sigma Chem.

In Vitro:

Article Title: Diastereoselective Synthesis of Potent Antimalarial Cis-β-lactam Agents
Article Snippet: General procedure for antimalarial activity measurements The chloroquine-resistant P. falciparum strain K14 (Southeast Asia) was cultured in vitro in complete medium consisting of RPMI 1640 (In Vitrogen) supplemented with 27.5 mM NaHCO3 , 20 mg/L gentamycin, and 10% human serum (19). .. Then, 100 µL from a culture containing > 95% ring (0-20 h postinvasion) at a 0.8% parasitemia and 3% haematocrit in complete medium was added per well along with 1.0 µCi of 3H-hypoxanthine with a specific activity of 14.1 Ci/mmol (Perkin-Elmer, Courtaboeuf, France).

Stable Transfection:

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: .. In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The affinities of drugs at the [3 H]WIN35,428 binding site on the DAT were determined by incubating tissue with a fixed concentration of [3 H]WIN35,428 and a range of drug concentrations.

Chromatography:

Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Article Snippet: Razor blade 1.57 mm OD polyethylene tubing ( e.g ., Clay Adams® Intramedic® , BD, catalog number: 427431) Sephadex microcentrifuge columns (Illustra Microspin G-25) (GE Healthcare, catalog number: 27-5325-01) Plastic wrap ( e.g. , Fisherbrand Clear Plastic Wrap, Fisher Scientific, catalog number: 22-305654) C-fold paper towels ( e.g. , Scott paper towels, KCWW, Kimberly-Clark, catalog number: 01510) Positively charged nylon DNA blotting membrane (Hybond-N+, 30.0×50.0 cm) (GE Healthcare, catalog number: RPN3050B) Chromatography transfer paper (Whatman 3MM, 46.0×57.0 cm) (GE Healthcare, catalog number: 3030-917) Syringe tip ( e.g ., B-D 18 G 1 ½ PrecisionGlide® Needle) (BD, catalog number: 305196) phiX174 virion DNA, 1 mg/ml (New England Biolabs, catalog number: N3023L) Phi29 DNA polymerase (New England Biolabs, catalog number: M0269S) 100 mM dNTP (deoxynucleotide triphosphate) set (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0181) 1 µM CMG ( C dc45 M cm2–7 G ins) helicase (see for purification details) pUC19, 1 mg/ml (New England Biolabs, catalog number: N3041L) Bsa I-HF with CutSmart buffer (New England Biolabs, catalog number: R3535L) ‘blockLd’ oligo* ‘blockLg’ oligo* ‘Pr1B’ oligo* ‘160Ld’ oligo* ‘91Lg’ oligo* ‘Fork primer’ oligo* Nucleotide-biased template (synthesized by Biomatik, Wilmington DE)* *Note: See . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

Synthesized:

Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Article Snippet: Razor blade 1.57 mm OD polyethylene tubing ( e.g ., Clay Adams® Intramedic® , BD, catalog number: 427431) Sephadex microcentrifuge columns (Illustra Microspin G-25) (GE Healthcare, catalog number: 27-5325-01) Plastic wrap ( e.g. , Fisherbrand Clear Plastic Wrap, Fisher Scientific, catalog number: 22-305654) C-fold paper towels ( e.g. , Scott paper towels, KCWW, Kimberly-Clark, catalog number: 01510) Positively charged nylon DNA blotting membrane (Hybond-N+, 30.0×50.0 cm) (GE Healthcare, catalog number: RPN3050B) Chromatography transfer paper (Whatman 3MM, 46.0×57.0 cm) (GE Healthcare, catalog number: 3030-917) Syringe tip ( e.g ., B-D 18 G 1 ½ PrecisionGlide® Needle) (BD, catalog number: 305196) phiX174 virion DNA, 1 mg/ml (New England Biolabs, catalog number: N3023L) Phi29 DNA polymerase (New England Biolabs, catalog number: M0269S) 100 mM dNTP (deoxynucleotide triphosphate) set (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0181) 1 µM CMG ( C dc45 M cm2–7 G ins) helicase (see for purification details) pUC19, 1 mg/ml (New England Biolabs, catalog number: N3041L) Bsa I-HF with CutSmart buffer (New England Biolabs, catalog number: R3535L) ‘blockLd’ oligo* ‘blockLg’ oligo* ‘Pr1B’ oligo* ‘160Ld’ oligo* ‘91Lg’ oligo* ‘Fork primer’ oligo* Nucleotide-biased template (synthesized by Biomatik, Wilmington DE)* *Note: See . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

Recombinase Polymerase Amplification:

Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Article Snippet: .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

Size-exclusion Chromatography:

Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Article Snippet: T4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 5 M NaCl (Sigma-Aldrich, catalog number: S9888) Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog number: 17012001) 1 kb MW marker (New England Biolabs, catalog number: N3232L) Ethidium bromide (EthBr, 10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) T4 kinase and 10× T4 kinase buffer (New England Biolabs, catalog number: M0201L) 32 P-γ-ATP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU002A) Type XI low-melt agarose (Sigma-Aldrich, catalog number: A3038) Note: This product has been discontinued . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

Cell Culture:

Article Title: Diastereoselective Synthesis of Potent Antimalarial Cis-β-lactam Agents
Article Snippet: General procedure for antimalarial activity measurements The chloroquine-resistant P. falciparum strain K14 (Southeast Asia) was cultured in vitro in complete medium consisting of RPMI 1640 (In Vitrogen) supplemented with 27.5 mM NaHCO3 , 20 mg/L gentamycin, and 10% human serum (19). .. Then, 100 µL from a culture containing > 95% ring (0-20 h postinvasion) at a 0.8% parasitemia and 3% haematocrit in complete medium was added per well along with 1.0 µCi of 3H-hypoxanthine with a specific activity of 14.1 Ci/mmol (Perkin-Elmer, Courtaboeuf, France).

Purification:

Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Article Snippet: .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

Concentration Assay:

Article Title: Diastereoselective Synthesis of Potent Antimalarial Cis-β-lactam Agents
Article Snippet: Increasing concentrations of lactam derivatives (100 µL/well, top concentration = 50 µM) were distributed in a 96-well plate; DMSO (0.5% vol/vol, top concentration) was distributed for control. .. Then, 100 µL from a culture containing > 95% ring (0-20 h postinvasion) at a 0.8% parasitemia and 3% haematocrit in complete medium was added per well along with 1.0 µCi of 3H-hypoxanthine with a specific activity of 14.1 Ci/mmol (Perkin-Elmer, Courtaboeuf, France).

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The affinities of drugs at the [3 H]WIN35,428 binding site on the DAT were determined by incubating tissue with a fixed concentration of [3 H]WIN35,428 and a range of drug concentrations.

Incubation:

Article Title: Docking Screens for Dual Inhibitors of Disparate Drug Targets for Parkinson’s Disease
Article Snippet: .. An amount of 5 μg of membranes from HeLa-A2A cell line was incubated with 3 nM [3 H]ZM241385 (4-[2-[[7-amino-2-(furan-2-yl)-[1,2,4]triazolo[1,5-a ][1,3,5]triazin-5-yl]amino]ethyl]-2-tritiophenol) (50 Ci/mmol, 1 mCi/ml, ARC-ITISA 0884) and the studied compound at 25 °C for 30 min and then filtered and washed four times with 250 μL of wash buffer (Tris-HCl 50 mM, EDTA 1 mM, MgCl2 10 mM, pH = 7.4), before measuring in a microplate β scintillation counter (Microbeta Trilux, PerkinElmer, Madrid, Spain). .. Nonspecific binding was determined in the presence of 50 μM NECA (Sigma E2387).

Article Title: Altered Ratio of D1 and D2 Dopamine Receptors in Mouse Striatum Is Associated with Behavioral Sensitization to Cocaine
Article Snippet: D1R number was measured using [3 H]-N -methyl-SCH23390 ([3 H]-SCH23390, 85 Ci/mmol, Perkin Elmer, Boston, MA). .. Samples were incubated for 60 minutes at 37°C and filtered through Whatman GF/C filters that had been pretreated with 0.3% Poly(ethyleneimine) (PEI, Sigma).

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The 1 h incubation at 22 °C was initiated by addition of membranes and terminated by rapid filtration over Whatman GF/B glass fiber filters pre-soaked in 0.1% polyethyleneimine (Sigma Chem.

other:

Article Title: Hypoxia and hyperoxia potentiate PAF receptor‐mediated effects in newborn ovine pulmonary arterial smooth muscle cells: significance in oxygen therapy of PPHN. Hypoxia and hyperoxia potentiate PAF receptor‐mediated effects in newborn ovine pulmonary arterial smooth muscle cells: significance in oxygen therapy of PPHN
Article Snippet: Authentic standards of PAF hexadecyl‐2‐acetyl‐sn‐glyceryl‐3‐phosphorylcholine (C16 ‐PAF) and lyso‐C16 ‐PAF standards as well as 8‐Br‐cAMP, Rp‐cAMPS were purchased from Biomol, Plymouth Meeting, PA. Radiolabeled PAF standards and substrates; hexadecyl‐2‐acetyl‐sn‐glyceryl‐3‐phosphorylcholine, 1‐O‐[acetyl‐3 H‐(N)]‐ (3 H‐acetyl‐C16 ‐PAF), 21.5 Ci/mmol (370 GBq/mmol) were purchased from Perkin Elmer Life Sciences (Boston, MA).

Article Title: Inhibition of Mycoplasma pneumoniae growth by FDA-approved anticancer and antiviral nucleoside and nucleobase analogs
Article Snippet: Materials Radiolabelled substances: [3 H]-hypoxanthine ([3 H]-Hx, 13.8 Ci/mmol), [3 H]-thymidine ([3 H]-dT, 20 Ci/mmol), [γ-32 P]-ATP (2000 Ci/mmol), [3 H]-Uracil ([3 H]-Ura, 31.65 Ci/mmol), and [3 H]-adenine ([3 H]-Ade, 27.2 Ci/mmol) were purchased from PerkinElmer.

Article Title: Neonatal Diabetes and Congenital Hyperinsulinism Caused by Mutations in ABCC8/SUR1 are Associated with Altered and Opposite Affinities for ATP and ADP
Article Snippet: Reagents [3 H]glibenclamide, 5-chloro-N -(4-[N -(cyclohexylcarbamoyl) sulfamoyl]phenethyl)-2-methoxybenzamide, NET1024250UC at 40–70 ci/mmol was purchased from Perkin-Elmer, Inc., Waltham, MA, USA.

Article Title: Pre-Symptomatic Activation of Antioxidant Responses and Alterations in Glucose and Pyruvate Metabolism in Niemann-Pick Type C1-Deficient Murine Brain
Article Snippet: [3 H]-deoxyglucose (2-[1,2-3 H(N)]; 1 mCi/ml; 25–50 Ci/mmol) was obtained from Perkin-Elmer (Waltham, MA).

Activity Assay:

Article Title: Diastereoselective Synthesis of Potent Antimalarial Cis-β-lactam Agents
Article Snippet: .. Then, 100 µL from a culture containing > 95% ring (0-20 h postinvasion) at a 0.8% parasitemia and 3% haematocrit in complete medium was added per well along with 1.0 µCi of 3H-hypoxanthine with a specific activity of 14.1 Ci/mmol (Perkin-Elmer, Courtaboeuf, France). ..

Article Title: Novel N-1,2-Dihydroxypropyl Analogs of Lobelane Inhibit Vesicular Monoamine Transporter-2 Function and Methamphetamine-Evoked Dopamine Release
Article Snippet: .. [3 H]Dopamine ([3 H]DA; dihydroxyphenylethylamine, 3,4-[7-3 H]; specific activity, 28 Ci/mmol), [3 H]5-hydroxytryptamine ([3 H]5-HT; hydroxytryptamine creatinine sulfate 5-[1,2-3 H(N)]; specific activity, 30 Ci/mmol), and Microscint 20 LSC-cocktail were purchased from PerkinElmer Life and Analytical Sciences (Waltham, MA). .. [3 H]DTBZ [(±)α-[ O-methyl -3 H]dihydrotetrabenazine; specific activity, 20 Ci/mmol] was obtained from American Radiolabeled Chemicals (St. Louis, MO).

Labeling:

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: .. In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The affinities of drugs at the [3 H]WIN35,428 binding site on the DAT were determined by incubating tissue with a fixed concentration of [3 H]WIN35,428 and a range of drug concentrations.

Expressing:

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: .. In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The affinities of drugs at the [3 H]WIN35,428 binding site on the DAT were determined by incubating tissue with a fixed concentration of [3 H]WIN35,428 and a range of drug concentrations.

Marker:

Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Article Snippet: T4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 5 M NaCl (Sigma-Aldrich, catalog number: S9888) Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog number: 17012001) 1 kb MW marker (New England Biolabs, catalog number: N3232L) Ethidium bromide (EthBr, 10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) T4 kinase and 10× T4 kinase buffer (New England Biolabs, catalog number: M0201L) 32 P-γ-ATP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU002A) Type XI low-melt agarose (Sigma-Aldrich, catalog number: A3038) Note: This product has been discontinued . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

Binding Assay:

Article Title: Docking Screens for Dual Inhibitors of Disparate Drug Targets for Parkinson’s Disease
Article Snippet: Paragraph title: Binding Assays for the Adenosine Receptors ... An amount of 5 μg of membranes from HeLa-A2A cell line was incubated with 3 nM [3 H]ZM241385 (4-[2-[[7-amino-2-(furan-2-yl)-[1,2,4]triazolo[1,5-a ][1,3,5]triazin-5-yl]amino]ethyl]-2-tritiophenol) (50 Ci/mmol, 1 mCi/ml, ARC-ITISA 0884) and the studied compound at 25 °C for 30 min and then filtered and washed four times with 250 μL of wash buffer (Tris-HCl 50 mM, EDTA 1 mM, MgCl2 10 mM, pH = 7.4), before measuring in a microplate β scintillation counter (Microbeta Trilux, PerkinElmer, Madrid, Spain).

Article Title: Altered Ratio of D1 and D2 Dopamine Receptors in Mouse Striatum Is Associated with Behavioral Sensitization to Cocaine
Article Snippet: Paragraph title: Radioligand binding ... D1R number was measured using [3 H]-N -methyl-SCH23390 ([3 H]-SCH23390, 85 Ci/mmol, Perkin Elmer, Boston, MA).

Article Title: The synthesis of bivalent 2?-carbomethoxy-3?-(3,4-dichlorophenyl)-8-heterobicyclo[3.2.1]octanes as probes for proximal binding sites on the dopamine and serotonin transporters
Article Snippet: In assays involving the human transporters, membranes from transfected cells stably expressing the DAT were labeled with [3 H]WIN35,428 ([3 H]CFT, 2β-carbomethoxy-3β-(4-fluorophenyl)-N-[3 H]methyltropane, 81 − 84 Ci/mmol, Perkin Elmer, Boston , MA). .. The affinities of drugs at the [3 H]WIN35,428 binding site on the DAT were determined by incubating tissue with a fixed concentration of [3 H]WIN35,428 and a range of drug concentrations.

Article Title: In Vivo Phenotypic Screening for Treating Chronic Neuropathic Pain: Modification of C2-Arylethynyl Group of Conformationally Constrained A3 Adenosine Receptor Agonists
Article Snippet: .. Radioligand Binding Studies [3 H]R -N 6 -Phenylisopropyladenosine ( 65 , [3 H]R-PIA, 63 Ci/mmol), [3 H](2-[p -(2-carboxyethyl)phenyl-ethylamino]-5′-N -ethylcarboxamido-adenosine), ( 66 , [3 H]CGS21680, 40.5 Ci/mmol), and [125 I]N 6 -(4-amino-3-iodobenzyl)adenosine-5′-N -methyluronamide ( 67 , [125 I]I-AB-MECA, 2200 Ci/mmol) were purchased from Perkin–Elmer Life and Analytical Science (Boston, MA). ..

Mouse Assay:

Article Title: Altered Ratio of D1 and D2 Dopamine Receptors in Mouse Striatum Is Associated with Behavioral Sensitization to Cocaine
Article Snippet: Radioligand binding 24 hours after the last drug administration, mice were sacrificed by cervical dislocation and their striatum harvested for analysis of D1R and D2R. .. D1R number was measured using [3 H]-N -methyl-SCH23390 ([3 H]-SCH23390, 85 Ci/mmol, Perkin Elmer, Boston, MA).

Inhibition:

Article Title: Diastereoselective Synthesis of Potent Antimalarial Cis-β-lactam Agents
Article Snippet: Then, 100 µL from a culture containing > 95% ring (0-20 h postinvasion) at a 0.8% parasitemia and 3% haematocrit in complete medium was added per well along with 1.0 µCi of 3H-hypoxanthine with a specific activity of 14.1 Ci/mmol (Perkin-Elmer, Courtaboeuf, France). .. Percentage growth inhibition was calculated from the parasite-associated radioactivity.

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  • 92
    PerkinElmer ci mmol 32 p α dgtp
    Telomerase elongation of primers with a terminal 8-oxoG. ( a ) Telomerase reactions were conducted using primers containing three (3R) or four (4R) TTAGGG repeats, with a 3′ terminal G or 8-oxoG ( Supplementary Table 1 , oligos #3, 4, 6 and 7). Reactions contained high (lanes 1 – 4) or cellular (lanes 5 – 8) dNTP concentrations and 0.3 μM 32 <t>P-α-dGTP.</t> Telomerase products were separated on denaturing gels. The LC was 32 P end-labeled 36-mer oligonucleotide. Arrow points to a product from degraded primer. Numbers with plus sign indicate number of added repeats. ( b ) and ( c ) Products were normalized to the LC and used to calculate processivity and relative activity. Bars represent the mean ± sd from three independent reactions. ** p
    Ci Mmol 32 P α Dgtp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ci mmol 32 p α dgtp/product/PerkinElmer
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ci mmol 32 p α dgtp - by Bioz Stars, 2020-04
    92/100 stars
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    99
    PerkinElmer ci mmol
    Telomerase elongation of primers with a terminal 8-oxoG. ( a ) Telomerase reactions were conducted using primers containing three (3R) or four (4R) TTAGGG repeats, with a 3′ terminal G or 8-oxoG ( Supplementary Table 1 , oligos #3, 4, 6 and 7). Reactions contained high (lanes 1 – 4) or cellular (lanes 5 – 8) dNTP concentrations and 0.3 μM 32 <t>P-α-dGTP.</t> Telomerase products were separated on denaturing gels. The LC was 32 P end-labeled 36-mer oligonucleotide. Arrow points to a product from degraded primer. Numbers with plus sign indicate number of added repeats. ( b ) and ( c ) Products were normalized to the LC and used to calculate processivity and relative activity. Bars represent the mean ± sd from three independent reactions. ** p
    Ci Mmol, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ci mmol/product/PerkinElmer
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ci mmol - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Telomerase elongation of primers with a terminal 8-oxoG. ( a ) Telomerase reactions were conducted using primers containing three (3R) or four (4R) TTAGGG repeats, with a 3′ terminal G or 8-oxoG ( Supplementary Table 1 , oligos #3, 4, 6 and 7). Reactions contained high (lanes 1 – 4) or cellular (lanes 5 – 8) dNTP concentrations and 0.3 μM 32 P-α-dGTP. Telomerase products were separated on denaturing gels. The LC was 32 P end-labeled 36-mer oligonucleotide. Arrow points to a product from degraded primer. Numbers with plus sign indicate number of added repeats. ( b ) and ( c ) Products were normalized to the LC and used to calculate processivity and relative activity. Bars represent the mean ± sd from three independent reactions. ** p

    Journal: Nature structural & molecular biology

    Article Title: Oxidative guanine base damage regulates human telomerase activity

    doi: 10.1038/nsmb.3319

    Figure Lengend Snippet: Telomerase elongation of primers with a terminal 8-oxoG. ( a ) Telomerase reactions were conducted using primers containing three (3R) or four (4R) TTAGGG repeats, with a 3′ terminal G or 8-oxoG ( Supplementary Table 1 , oligos #3, 4, 6 and 7). Reactions contained high (lanes 1 – 4) or cellular (lanes 5 – 8) dNTP concentrations and 0.3 μM 32 P-α-dGTP. Telomerase products were separated on denaturing gels. The LC was 32 P end-labeled 36-mer oligonucleotide. Arrow points to a product from degraded primer. Numbers with plus sign indicate number of added repeats. ( b ) and ( c ) Products were normalized to the LC and used to calculate processivity and relative activity. Bars represent the mean ± sd from three independent reactions. ** p

    Article Snippet: Briefly, reactions (20 μl) contained 1x human telomerase buffer, 1 μM oligonucleotide substrate, 0.3 μM of 3,000 Ci/mmol 32 P-α-dGTP or 32 P-α-dTTP (Perkin Elmer) and dNTP (Invitrogen) mix as indicated in the figure legend.

    Techniques: Labeling, Activity Assay

    8-oxoG restores telomerase activity on quadruplex folded overhangs ( a ) Telomerase reactions were conducted using primers containing three (3R) or four (4R) TTAGGG repeats, with a middle G or 8-oxoG in the terminal repeat ( Supplementary Table 1 , oligos #3, 5, 6 and 8). Reactions contained high (lanes 1–4) or cellular (lanes 5–8) dNTP concentrations and 0.3 μM 32 P-α-dGTP. Products were separated on denaturing gels. The LC was a 32 P end-labeled 36-mer oligonucleotide. Numbers with plus sign indicate number of added repeats. ( b ) and ( c ) Products were normalized to the LC, and used to calculate processivity ( b ) and relative activity ( c ). Bars represent the mean ± sd from three independent reactions. * p

    Journal: Nature structural & molecular biology

    Article Title: Oxidative guanine base damage regulates human telomerase activity

    doi: 10.1038/nsmb.3319

    Figure Lengend Snippet: 8-oxoG restores telomerase activity on quadruplex folded overhangs ( a ) Telomerase reactions were conducted using primers containing three (3R) or four (4R) TTAGGG repeats, with a middle G or 8-oxoG in the terminal repeat ( Supplementary Table 1 , oligos #3, 5, 6 and 8). Reactions contained high (lanes 1–4) or cellular (lanes 5–8) dNTP concentrations and 0.3 μM 32 P-α-dGTP. Products were separated on denaturing gels. The LC was a 32 P end-labeled 36-mer oligonucleotide. Numbers with plus sign indicate number of added repeats. ( b ) and ( c ) Products were normalized to the LC, and used to calculate processivity ( b ) and relative activity ( c ). Bars represent the mean ± sd from three independent reactions. * p

    Article Snippet: Briefly, reactions (20 μl) contained 1x human telomerase buffer, 1 μM oligonucleotide substrate, 0.3 μM of 3,000 Ci/mmol 32 P-α-dGTP or 32 P-α-dTTP (Perkin Elmer) and dNTP (Invitrogen) mix as indicated in the figure legend.

    Techniques: Activity Assay, Labeling