Structured Review

Waters Corporation chromatography tandem mass spectrometry
Proteomic analysis of NCM460 and HCT 116 using a bottom-up proteomic strategy. Proteins were analyzed by label-free quantification (LFQ) liquid <t>chromatography–tandem</t> <t>mass</t> <t>spectrometry</t> (LC–MS/MS). MaxQuant and Perseus were used for data analysis. The log 2 transformed relative expression levels of HCT 116 versus NCM460 are plotted on the x-axis and on the y-axis are plotted the log 10 transformed p -values. Unchanged proteins are indicated with grey circles. 901 proteins were significantly ( p
Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 111 article reviews
Price from $9.99 to $1999.99
chromatography tandem mass spectrometry - by Bioz Stars, 2020-09
93/100 stars

Images

1) Product Images from "Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer"

Article Title: Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21103466

Proteomic analysis of NCM460 and HCT 116 using a bottom-up proteomic strategy. Proteins were analyzed by label-free quantification (LFQ) liquid chromatography–tandem mass spectrometry (LC–MS/MS). MaxQuant and Perseus were used for data analysis. The log 2 transformed relative expression levels of HCT 116 versus NCM460 are plotted on the x-axis and on the y-axis are plotted the log 10 transformed p -values. Unchanged proteins are indicated with grey circles. 901 proteins were significantly ( p
Figure Legend Snippet: Proteomic analysis of NCM460 and HCT 116 using a bottom-up proteomic strategy. Proteins were analyzed by label-free quantification (LFQ) liquid chromatography–tandem mass spectrometry (LC–MS/MS). MaxQuant and Perseus were used for data analysis. The log 2 transformed relative expression levels of HCT 116 versus NCM460 are plotted on the x-axis and on the y-axis are plotted the log 10 transformed p -values. Unchanged proteins are indicated with grey circles. 901 proteins were significantly ( p

Techniques Used: Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Transformation Assay, Expressing

2) Product Images from "Omega-3 Polyunsaturated Fatty Acids Decrease Aortic Valve Disease Through the Resolvin E1 and ChemR23 Axis"

Article Title: Omega-3 Polyunsaturated Fatty Acids Decrease Aortic Valve Disease Through the Resolvin E1 and ChemR23 Axis

Journal: Circulation

doi: 10.1161/CIRCULATIONAHA.119.041868

Omega-3 polyunsaturated fatty acids (PUFAs) are higher and n-6 PUFAs are lower in valve leaflets of Fat-1 tg ×Apoe −/− compared with Apoe −/− mice. A , Optical micrograph of an aortic root section of a Fat-1 tg ×Apoe −/− mouse. Red squares indicate areas (500×500 µm 2 ) for focused analyses of valve leaflets and valve insertion, respectively. B and C , Time-of-flight secondary ion mass spectrometry (TOF-SIMS) data from 2 different regions of the valve leaflet area with ion images of ( left ) overlay image of phosphatidylethanolamine (PE) in red, cholesterol in green, and heme in blue ( left ); total ion image with region of interest (ROI) used for extraction of mass spectrum of valve leaflet indicated in gray ( middle ); and image of the added signal intensity of eicosapentaenoic acid (EPA; C20:5n3), docosahexaenoic acid (DHA; C22:6n3), and docosapentaenoic acid (DPA, C22:5n3; right ). Brighter pixels in the ion images correspond to higher signal intensities. D , n-3 PUFAs (DHA, EPA, and DPA) and ( E ) n-6 PUFAs (arachidonic acid [C20:4n6] and adrenic acid [C22:4n6]) normalized TOF-SIMS signal intensities (n=3 animals per group; each observation is the average of 3 independent leaflet regions at different valve levels) in mass spectra acquired from valve leaflets. F , HS-omega-3 index in ventricular myocardium measured by gas chromatography in Apoe −/− compared with Fat-1 tg ×Apoe −/− mice (n=6 per group). Data are presented as individual values with horizontal lines representing mean±SEM. Statistical significances were evaluated with either a Student t test or a 2-way repeated measures ANOVA followed by Holm-Sidak multiple-comparison test. * P
Figure Legend Snippet: Omega-3 polyunsaturated fatty acids (PUFAs) are higher and n-6 PUFAs are lower in valve leaflets of Fat-1 tg ×Apoe −/− compared with Apoe −/− mice. A , Optical micrograph of an aortic root section of a Fat-1 tg ×Apoe −/− mouse. Red squares indicate areas (500×500 µm 2 ) for focused analyses of valve leaflets and valve insertion, respectively. B and C , Time-of-flight secondary ion mass spectrometry (TOF-SIMS) data from 2 different regions of the valve leaflet area with ion images of ( left ) overlay image of phosphatidylethanolamine (PE) in red, cholesterol in green, and heme in blue ( left ); total ion image with region of interest (ROI) used for extraction of mass spectrum of valve leaflet indicated in gray ( middle ); and image of the added signal intensity of eicosapentaenoic acid (EPA; C20:5n3), docosahexaenoic acid (DHA; C22:6n3), and docosapentaenoic acid (DPA, C22:5n3; right ). Brighter pixels in the ion images correspond to higher signal intensities. D , n-3 PUFAs (DHA, EPA, and DPA) and ( E ) n-6 PUFAs (arachidonic acid [C20:4n6] and adrenic acid [C22:4n6]) normalized TOF-SIMS signal intensities (n=3 animals per group; each observation is the average of 3 independent leaflet regions at different valve levels) in mass spectra acquired from valve leaflets. F , HS-omega-3 index in ventricular myocardium measured by gas chromatography in Apoe −/− compared with Fat-1 tg ×Apoe −/− mice (n=6 per group). Data are presented as individual values with horizontal lines representing mean±SEM. Statistical significances were evaluated with either a Student t test or a 2-way repeated measures ANOVA followed by Holm-Sidak multiple-comparison test. * P

Techniques Used: Mouse Assay, Mass Spectrometry, Gas Chromatography

Resolvin E1 (RvE1) is dysregulated in human calcified valve tissue. A , Representative multiple reaction monitoring chromatograms depicting the relative abundance of RvE1 and resolvin D3 (RvD3; Q1 > Q3), and accompanying tandem mass spectrometry spectra used in the identification of RvE1 (inset, diagnostic ions). B through D , RvE1, RvD3, and leukotriene B4 (LTB 4 ) levels from aortic valve tissue determined by liquid chromatography tandem mass spectrometry–based lipid mediator lipidomics. Data are presented as individual values, and statistical significance was evaluated by paired Student t test (n=9 vs n=9). M indicates molecular mass. * P
Figure Legend Snippet: Resolvin E1 (RvE1) is dysregulated in human calcified valve tissue. A , Representative multiple reaction monitoring chromatograms depicting the relative abundance of RvE1 and resolvin D3 (RvD3; Q1 > Q3), and accompanying tandem mass spectrometry spectra used in the identification of RvE1 (inset, diagnostic ions). B through D , RvE1, RvD3, and leukotriene B4 (LTB 4 ) levels from aortic valve tissue determined by liquid chromatography tandem mass spectrometry–based lipid mediator lipidomics. Data are presented as individual values, and statistical significance was evaluated by paired Student t test (n=9 vs n=9). M indicates molecular mass. * P

Techniques Used: Mass Spectrometry, Diagnostic Assay, Liquid Chromatography

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High Performance Liquid Chromatography:

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Article Snippet: .. After cell lysis, supernatants were collected and analyzed by liquid chromatography-tandem mass spectrometry on a Finnigan TSQ Quantum Discovery MAX on a Quattro-Micro triple quadrupole mass spectrometer (Waters Corporation, Milford, MA), coupled with an Alliance high-performance liquid chromatography (HPLC) system (Waters Corporation). .. The MassLynx software from Waters was used for instrument control, data acquisition, and data processing. c-di-GMP was detected by using selected reaction monitoring (SRM) in negative ionization mode at m/z 689.0/150.1.

Lysis:

Article Title: Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073
Article Snippet: .. After cell lysis, supernatants were collected and analyzed by liquid chromatography-tandem mass spectrometry on a Finnigan TSQ Quantum Discovery MAX on a Quattro-Micro triple quadrupole mass spectrometer (Waters Corporation, Milford, MA), coupled with an Alliance high-performance liquid chromatography (HPLC) system (Waters Corporation). .. The MassLynx software from Waters was used for instrument control, data acquisition, and data processing. c-di-GMP was detected by using selected reaction monitoring (SRM) in negative ionization mode at m/z 689.0/150.1.

Chromatography:

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Article Title: Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073
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Article Title: Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer
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Mass Spectrometry:

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Article Title: Altered Regulation of the Diguanylate Cyclase YaiC Reduces Production of Type 1 Fimbriae in a Pst Mutant of Uropathogenic Escherichia coli CFT073
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Article Snippet: .. Samples were homogenized and proteins were precipitated, followed by solid-phase extraction with Isolute C18 columns (Biotage, Uppsala, Sweden) and targeted liquid chromatography–tandem mass spectrometry as previously described., The system consisted of Waters XevoTQS triple quadruple equipped with Acquity UPLC System from Waters Corporation and an autosampler cooled to 5°C (Milford, MA). ..

Liquid Chromatography:

Article Title: Fipronil application on rice paddy fields reduces densities of common skimmer and scarlet skimmer
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Article Title: The acute effect of metabolic cofactor supplementation: a potential therapeutic strategy against non‐alc33oholic fatty liver disease
Article Snippet: .. Briefly, the liquid chromatography–tandem mass spectrometry (LC‐MS/MS) platform was based on a Waters ACQUITY ultraperformance liquid chromatography (UPLC) system and a Thermo‐Finnigan LTQ mass spectrometer operated at nominal mass resolution, which was equipped with an electrospray ionization (ESI) source and a linear ion trap (LIT) mass analyzer. .. The sample extract was dried and then reconstituted in acidic or basic LC‐compatible solvents, each of which contained 12 or more injection standards at fixed concentrations.

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    Waters Corporation sim lc ms ms
    Analysis of <t>HiPMO1</t> reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using <t>SIM</t> LC–MS/MS analysis. a SIM LC–MS showing extracted ion chromatograms and the corresponding mass spectra of glucuronic acid and saccharic acid (saccharic acid lactone). In positive mode: saccharic acid ( m / z 210 + H + ). In negative mode: glucuronic acid ( m / z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ). b The SIM MS/MS spectra showing fragmentation ions of the parent ions at m / z 192.8 for glucuronic acid ( m / z 194 − H + ) and at m / z 190.9 for saccharic acid lactone ( m / z 192 − H + ). Loss of [H] and [H 2 O] and addition of [H] is common in carbohydrate fragmentations using LC–MS/MS in the negative ion mode [ 38 ]. The loss of [H], [H 2 O], [CHO], and [COOH] and the addition of [H] from the parent ions of glucuronic acid ( m/z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ), generating various fragmentation ions. The MS/MS spectra of saccharic acid ( m / z 210 + H + ) were not shown, likely due to the low intensities
    Sim Lc Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Waters Corporation γ hbcdd
    Analytical scale LC-MS separation of ( A ) a technical <t>HBCDD</t> mixture and ( B ) the isolated α-, β-, and <t>γ-HBCDD</t> enantiomers as well as reference standards for δ- and ε-HBCDD on a Phenomenex Nucleodex β-PM (permethylated β-cyclodextrin) stationary phase at ambient temperature using an acetonitrile/water mobile phase.
    γ Hbcdd, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation waters xbridge c18
    Extracted ion chromatograms of 31 standard compounds separated by reverse-phase chromatography using a (A) <t>XBridge</t> <t>C18,</t> (B) Cogent Bidentate C18, and (C) Scherzo SM-C18 column.
    Waters Xbridge C18, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Waters Corporation urinary vitamin e metabolites
    Product ion scans of <t>vitamin</t> E metabolites.
    Urinary Vitamin E Metabolites, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using SIM LC–MS/MS analysis. a SIM LC–MS showing extracted ion chromatograms and the corresponding mass spectra of glucuronic acid and saccharic acid (saccharic acid lactone). In positive mode: saccharic acid ( m / z 210 + H + ). In negative mode: glucuronic acid ( m / z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ). b The SIM MS/MS spectra showing fragmentation ions of the parent ions at m / z 192.8 for glucuronic acid ( m / z 194 − H + ) and at m / z 190.9 for saccharic acid lactone ( m / z 192 − H + ). Loss of [H] and [H 2 O] and addition of [H] is common in carbohydrate fragmentations using LC–MS/MS in the negative ion mode [ 38 ]. The loss of [H], [H 2 O], [CHO], and [COOH] and the addition of [H] from the parent ions of glucuronic acid ( m/z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ), generating various fragmentation ions. The MS/MS spectra of saccharic acid ( m / z 210 + H + ) were not shown, likely due to the low intensities

    Journal: Biotechnology for Biofuels

    Article Title: Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides

    doi: 10.1186/s13068-019-1384-0

    Figure Lengend Snippet: Analysis of HiPMO1 reaction products hydrolyzed by beta-glucuronidase and beta-glucosidase using SIM LC–MS/MS analysis. a SIM LC–MS showing extracted ion chromatograms and the corresponding mass spectra of glucuronic acid and saccharic acid (saccharic acid lactone). In positive mode: saccharic acid ( m / z 210 + H + ). In negative mode: glucuronic acid ( m / z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ). b The SIM MS/MS spectra showing fragmentation ions of the parent ions at m / z 192.8 for glucuronic acid ( m / z 194 − H + ) and at m / z 190.9 for saccharic acid lactone ( m / z 192 − H + ). Loss of [H] and [H 2 O] and addition of [H] is common in carbohydrate fragmentations using LC–MS/MS in the negative ion mode [ 38 ]. The loss of [H], [H 2 O], [CHO], and [COOH] and the addition of [H] from the parent ions of glucuronic acid ( m/z 194 − H + ) and saccharic acid lactone ( m / z 192 − H + ), generating various fragmentation ions. The MS/MS spectra of saccharic acid ( m / z 210 + H + ) were not shown, likely due to the low intensities

    Article Snippet: The full scan m /z ranged from 100 to 300. (ii) SIM LC–MS/MS: We analyzed HiPMO1 reaction products using SIM LC–MS (ACQUITY UPLC and Q-TOF MS Premier, Waters) on a U3000-HPLC C18 column (Agilent Zorbax SB-C18, 150 × 4.6 mm).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Analytical scale LC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Phenomenex Nucleodex β-PM (permethylated β-cyclodextrin) stationary phase at ambient temperature using an acetonitrile/water mobile phase.

    Journal: Molecules

    Article Title: Enantioselective Analytical- and Preparative-Scale Separation of Hexabromocyclododecane Stereoisomers Using Packed Column Supercritical Fluid Chromatography

    doi: 10.3390/molecules21111509

    Figure Lengend Snippet: Analytical scale LC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Phenomenex Nucleodex β-PM (permethylated β-cyclodextrin) stationary phase at ambient temperature using an acetonitrile/water mobile phase.

    Article Snippet: Isolation of Enantiomerically Pure (+)- and (−)-α-, β-, and γ-HBCDD by Preparative-Scale pSFC Preparative-scale pSFC separations were carried out using a Waters Prep15 SFC system (Waters Corp., Milford, MA, USA) coupled with a Waters 3100 benchtop single quadrupole mass detector (Waters Corp., Milford, MA, USA) configured in negative ion atmospheric pressure chemical ionization (APCI) mode with the following parameters: corona needle (uA) = 5.00, cone voltage (V) = 30.00; desolvation gas flow (L/h) = 550; desolvation temperature (°C) = 350; source temperature (°C) = 125.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Isolation

    Analytical scale pSFC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Trefoil CEL2 [cellulose tris-(3-chloro-4-methylphenylcarbamate)] stationary phase at 50 °C using an isopropanol modified carbon dioxide mobile phase.

    Journal: Molecules

    Article Title: Enantioselective Analytical- and Preparative-Scale Separation of Hexabromocyclododecane Stereoisomers Using Packed Column Supercritical Fluid Chromatography

    doi: 10.3390/molecules21111509

    Figure Lengend Snippet: Analytical scale pSFC-MS separation of ( A ) a technical HBCDD mixture and ( B ) the isolated α-, β-, and γ-HBCDD enantiomers as well as reference standards for δ- and ε-HBCDD on a Trefoil CEL2 [cellulose tris-(3-chloro-4-methylphenylcarbamate)] stationary phase at 50 °C using an isopropanol modified carbon dioxide mobile phase.

    Article Snippet: Isolation of Enantiomerically Pure (+)- and (−)-α-, β-, and γ-HBCDD by Preparative-Scale pSFC Preparative-scale pSFC separations were carried out using a Waters Prep15 SFC system (Waters Corp., Milford, MA, USA) coupled with a Waters 3100 benchtop single quadrupole mass detector (Waters Corp., Milford, MA, USA) configured in negative ion atmospheric pressure chemical ionization (APCI) mode with the following parameters: corona needle (uA) = 5.00, cone voltage (V) = 30.00; desolvation gas flow (L/h) = 550; desolvation temperature (°C) = 350; source temperature (°C) = 125.

    Techniques: Mass Spectrometry, Isolation, Modification

    Stable conformations, schematic representations, and stereoviews of the crystal structures (when acquired) of enantiomerically pure (+)-α-HBCDD, (+)-γ-HBCDD, and (+)-β-HBCDD as well as the two minor isomers δ-HBCDD and ε-HBCDD.

    Journal: Molecules

    Article Title: Enantioselective Analytical- and Preparative-Scale Separation of Hexabromocyclododecane Stereoisomers Using Packed Column Supercritical Fluid Chromatography

    doi: 10.3390/molecules21111509

    Figure Lengend Snippet: Stable conformations, schematic representations, and stereoviews of the crystal structures (when acquired) of enantiomerically pure (+)-α-HBCDD, (+)-γ-HBCDD, and (+)-β-HBCDD as well as the two minor isomers δ-HBCDD and ε-HBCDD.

    Article Snippet: Isolation of Enantiomerically Pure (+)- and (−)-α-, β-, and γ-HBCDD by Preparative-Scale pSFC Preparative-scale pSFC separations were carried out using a Waters Prep15 SFC system (Waters Corp., Milford, MA, USA) coupled with a Waters 3100 benchtop single quadrupole mass detector (Waters Corp., Milford, MA, USA) configured in negative ion atmospheric pressure chemical ionization (APCI) mode with the following parameters: corona needle (uA) = 5.00, cone voltage (V) = 30.00; desolvation gas flow (L/h) = 550; desolvation temperature (°C) = 350; source temperature (°C) = 125.

    Techniques:

    Extracted ion chromatograms of 31 standard compounds separated by reverse-phase chromatography using a (A) XBridge C18, (B) Cogent Bidentate C18, and (C) Scherzo SM-C18 column.

    Journal: Analytical Chemistry

    Article Title: Expanding Coverage of the Metabolome for Global Metabolite Profiling

    doi: 10.1021/ac102981k

    Figure Lengend Snippet: Extracted ion chromatograms of 31 standard compounds separated by reverse-phase chromatography using a (A) XBridge C18, (B) Cogent Bidentate C18, and (C) Scherzo SM-C18 column.

    Article Snippet: E.coli extractions and standard mixtures were separated using a Cogent Bidentate C18: 4 μm, 100Å, 150mm × 2.1 mm ID (Cat No. 40018-15P-2), a Waters XBridge C18: 3.5 μm, 135Å, 150mm × 1.0 mm ID (Part No. 186003128), or an Imtakt Scherzo SM-C18: 3 μm, 13nm, 150mm × 2mm ID (Prod.

    Techniques: Reversed-phase Chromatography

    Quantification of 36 standard compounds analyzed in negative ionization mode (ESI−) using three different additives in the mobile phase: 1mM ammonium fluoride, 5mM ammonium acetate, or 0.1% formic acid. Compounds were separated by reverse-phase chromatography using a XBridge C18 column. Fold values indicate the difference in intensity between ammonium fluoride and the closest mobile phase. The intensity scale of the X axis is log 10.

    Journal: Analytical Chemistry

    Article Title: Expanding Coverage of the Metabolome for Global Metabolite Profiling

    doi: 10.1021/ac102981k

    Figure Lengend Snippet: Quantification of 36 standard compounds analyzed in negative ionization mode (ESI−) using three different additives in the mobile phase: 1mM ammonium fluoride, 5mM ammonium acetate, or 0.1% formic acid. Compounds were separated by reverse-phase chromatography using a XBridge C18 column. Fold values indicate the difference in intensity between ammonium fluoride and the closest mobile phase. The intensity scale of the X axis is log 10.

    Article Snippet: E.coli extractions and standard mixtures were separated using a Cogent Bidentate C18: 4 μm, 100Å, 150mm × 2.1 mm ID (Cat No. 40018-15P-2), a Waters XBridge C18: 3.5 μm, 135Å, 150mm × 1.0 mm ID (Part No. 186003128), or an Imtakt Scherzo SM-C18: 3 μm, 13nm, 150mm × 2mm ID (Prod.

    Techniques: Reversed-phase Chromatography

    (A) Venn diagram representing the total number of features from LC/MS data of E.coli samples extracted using the method “Boiling water”, and analyzed using ammonium acetate or ammonium fluoride enriched mobile phases. (B) Quantification of 39 metabolites from E.coli analyzed using ammonium acetate and ammonium fluoride enriched mobile phases. The intensity scale of the X axis is log 2. Metabolites were separated by reverse-phase chromatography using an XBridge C18 column and detected in negative ionization mode (ESI−). Identification is based on accurate mass and MS/MS data. Fold values indicate the difference in intensity between ammonium fluoride and ammonium acetate. Examples of unique metabolites detected with ammonium fluoride are also represented. Data points and error bars represent mean intensity values and s.d. for three replicates.

    Journal: Analytical Chemistry

    Article Title: Expanding Coverage of the Metabolome for Global Metabolite Profiling

    doi: 10.1021/ac102981k

    Figure Lengend Snippet: (A) Venn diagram representing the total number of features from LC/MS data of E.coli samples extracted using the method “Boiling water”, and analyzed using ammonium acetate or ammonium fluoride enriched mobile phases. (B) Quantification of 39 metabolites from E.coli analyzed using ammonium acetate and ammonium fluoride enriched mobile phases. The intensity scale of the X axis is log 2. Metabolites were separated by reverse-phase chromatography using an XBridge C18 column and detected in negative ionization mode (ESI−). Identification is based on accurate mass and MS/MS data. Fold values indicate the difference in intensity between ammonium fluoride and ammonium acetate. Examples of unique metabolites detected with ammonium fluoride are also represented. Data points and error bars represent mean intensity values and s.d. for three replicates.

    Article Snippet: E.coli extractions and standard mixtures were separated using a Cogent Bidentate C18: 4 μm, 100Å, 150mm × 2.1 mm ID (Cat No. 40018-15P-2), a Waters XBridge C18: 3.5 μm, 135Å, 150mm × 1.0 mm ID (Part No. 186003128), or an Imtakt Scherzo SM-C18: 3 μm, 13nm, 150mm × 2mm ID (Prod.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Reversed-phase Chromatography, Mass Spectrometry

    Product ion scans of vitamin E metabolites.

    Journal: Free Radical Biology & Medicine

    Article Title: Urinary conjugated ?-tocopheronolactone--a biomarker of oxidative stress in children with type 1 diabetes

    doi: 10.1016/j.freeradbiomed.2012.09.012

    Figure Lengend Snippet: Product ion scans of vitamin E metabolites.

    Article Snippet: The urinary vitamin E metabolites were desalted and/or separated before mass spectrometry using a Waters 2795XE high-performance liquid chromatography unit fitted with a 100 × 2.1-mm (5 μ) HyPURITY C8 column plus a guard column containing the same stationary phase (Phenomenex UK).

    Techniques:

    The LC–MS/MS analysis of vitamin E metabolites and internal standards.

    Journal: Free Radical Biology & Medicine

    Article Title: Urinary conjugated ?-tocopheronolactone--a biomarker of oxidative stress in children with type 1 diabetes

    doi: 10.1016/j.freeradbiomed.2012.09.012

    Figure Lengend Snippet: The LC–MS/MS analysis of vitamin E metabolites and internal standards.

    Article Snippet: The urinary vitamin E metabolites were desalted and/or separated before mass spectrometry using a Waters 2795XE high-performance liquid chromatography unit fitted with a 100 × 2.1-mm (5 μ) HyPURITY C8 column plus a guard column containing the same stationary phase (Phenomenex UK).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Parent ion scans of vitamin E metabolites.

    Journal: Free Radical Biology & Medicine

    Article Title: Urinary conjugated ?-tocopheronolactone--a biomarker of oxidative stress in children with type 1 diabetes

    doi: 10.1016/j.freeradbiomed.2012.09.012

    Figure Lengend Snippet: Parent ion scans of vitamin E metabolites.

    Article Snippet: The urinary vitamin E metabolites were desalted and/or separated before mass spectrometry using a Waters 2795XE high-performance liquid chromatography unit fitted with a 100 × 2.1-mm (5 μ) HyPURITY C8 column plus a guard column containing the same stationary phase (Phenomenex UK).

    Techniques: