chromatography solvent b  (Millipore)


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  • 99
    Name:
    Trifluoroacetic acid
    Description:

    Catalog Number:
    t1647
    Price:
    None
    Applications:
    TFA can be used as an ion-pairing reagent for reverse-phase chromatography.
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    Structured Review

    Millipore chromatography solvent b

    https://www.bioz.com/result/chromatography solvent b/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chromatography solvent b - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides
    Article Snippet: .. Solvents for chromatography such as acetonitrile and TFA (HPLC grade) were from Sigma-Aldrich. .. Ethanol and methanol were from Avantor Performance Materials (Center Valley, PA, U.S.A.). (3-Aminopropyl) triethoxysilane (APTES 2%) was from Sigma-Aldrich.

    other:

    Article Title: A Multi-Step Chromatographic Approach to Purify Radically Generated Ferulate Oligomers Reveals Naturally Occurring 5-5/8-8(Cyclic)-, 8-8(Noncyclic)/8-O-4-, and 5-5/8-8(Noncyclic)-Coupled Dehydrotriferulic Acids
    Article Snippet: Research chemicals were obtained from the following suppliers: Cu(I)Cl, copper(II)-tetramethylethylenediamine (TMEDA), HCl (37%), NaOH, ammonium chloride, ethyl acetate, and ethanol from Carl Roth GmbH (Karlsruhe, Germany); acetonitrile, diethyl ether, methanol, acetone, petroleum ether, tetrahydrofuran, and Na2 SO4 from VWR International (Radnor, PA, USA); acetone- d 6 , ferulic acid, [1-13 C]triethyl phosphonoacetate, and trifluoroacetic acid from Sigma-Aldrich (St. Louis, MO, USA); D2 O from deutero GmbH (Kastellaun, Germany); acetyl chloride and vanillin from Fluka (Buchs, Switzerland); formic acid from Merck KGaA (Darmstadt, Germany); NaHCO3 from Riedel-de Haën AG (Seelze, Germany); carbogen (5% CO2 ) from Air Liquide S.A. (Düsseldorf, Germany), and NaH (60% dispersion in oil) from Alfa Aesar (Karlsruhe, Germany).

    Chromatography:

    Article Title: Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides
    Article Snippet: .. Solvents for chromatography such as acetonitrile and TFA (HPLC grade) were from Sigma-Aldrich. .. Ethanol and methanol were from Avantor Performance Materials (Center Valley, PA, U.S.A.). (3-Aminopropyl) triethoxysilane (APTES 2%) was from Sigma-Aldrich.

    Titration:

    Article Title: Studies on citrullinated LL-37: detection in human airways, antibacterial effects and biophysical properties
    Article Snippet: .. The 50 µL syringe was filled with 200 µM LL-37 or 200 µM LL-37Cit5 in 0.1% TFA (Sigma-Aldrich) for titration into 164 µL of 62.5 µM E. coli O111:B4 LPS (InVivoGen, San Diego, CA, USA) in 0.1% TFA (Sigma-Aldrich). .. Data were analyzed with the NanoAnalyze software (TA Instruments-Waters LLC, New Castle, USA).

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  • 98
    Millipore sheep anti α msh antisera
    Preabsorption controls for POMC, β-endorphin, ACTH and <t>α-MSH</t> antisera. (A1, A2) Adjacent sections from a brain with high POMC levels in tanycytes demonstrates that preincubation of the POMC antiserum with the immunizing peptide abolishes all POMC immunolabeling in tanycytes and neurons. (B1-B6) Preabsorption control for the β-endorphin antiserum in adjacent sections from a brain with intermediate POMC levels in tanycytes. Specific labeling is present in α2 tanycyte cell bodies and processes (arrow, B2), and in β2 and γ tanycytes (B3). In the section reacted with the preabsorbed antiserum (B4-6), some background labeling remains in the location of α2 tanycyte cell bodies, but this signal is indistinct. (C1, C2) Preabsorption control for the ACTH antiserum; inset shows an ACTH-positive γ tanycyte. (D1, D2) After preincubation of the α-MSH antiserum with excess α-MSH, a neurofilament-like nonspecific labeling remains in the internal median eminence (arrowheads). 3v, third ventricle; ME, median eminence. Scale bar: 100μm; 50μm on B2 (for B2, B3, B5, B6, inset of C1).
    Sheep Anti α Msh Antisera, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep anti α msh antisera/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sheep anti α msh antisera - by Bioz Stars, 2020-07
    98/100 stars
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    99
    Millipore acetonitrile
    Preabsorption controls for POMC, β-endorphin, ACTH and <t>α-MSH</t> antisera. (A1, A2) Adjacent sections from a brain with high POMC levels in tanycytes demonstrates that preincubation of the POMC antiserum with the immunizing peptide abolishes all POMC immunolabeling in tanycytes and neurons. (B1-B6) Preabsorption control for the β-endorphin antiserum in adjacent sections from a brain with intermediate POMC levels in tanycytes. Specific labeling is present in α2 tanycyte cell bodies and processes (arrow, B2), and in β2 and γ tanycytes (B3). In the section reacted with the preabsorbed antiserum (B4-6), some background labeling remains in the location of α2 tanycyte cell bodies, but this signal is indistinct. (C1, C2) Preabsorption control for the ACTH antiserum; inset shows an ACTH-positive γ tanycyte. (D1, D2) After preincubation of the α-MSH antiserum with excess α-MSH, a neurofilament-like nonspecific labeling remains in the internal median eminence (arrowheads). 3v, third ventricle; ME, median eminence. Scale bar: 100μm; 50μm on B2 (for B2, B3, B5, B6, inset of C1).
    Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acetonitrile/product/Millipore
    Average 99 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    acetonitrile - by Bioz Stars, 2020-07
    99/100 stars
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    Preabsorption controls for POMC, β-endorphin, ACTH and α-MSH antisera. (A1, A2) Adjacent sections from a brain with high POMC levels in tanycytes demonstrates that preincubation of the POMC antiserum with the immunizing peptide abolishes all POMC immunolabeling in tanycytes and neurons. (B1-B6) Preabsorption control for the β-endorphin antiserum in adjacent sections from a brain with intermediate POMC levels in tanycytes. Specific labeling is present in α2 tanycyte cell bodies and processes (arrow, B2), and in β2 and γ tanycytes (B3). In the section reacted with the preabsorbed antiserum (B4-6), some background labeling remains in the location of α2 tanycyte cell bodies, but this signal is indistinct. (C1, C2) Preabsorption control for the ACTH antiserum; inset shows an ACTH-positive γ tanycyte. (D1, D2) After preincubation of the α-MSH antiserum with excess α-MSH, a neurofilament-like nonspecific labeling remains in the internal median eminence (arrowheads). 3v, third ventricle; ME, median eminence. Scale bar: 100μm; 50μm on B2 (for B2, B3, B5, B6, inset of C1).

    Journal: The Journal of comparative neurology

    Article Title: Variable proopiomelanocortin expression in tanycytes of the adult rat hypothalamus and pituitary stalk *

    doi: 10.1002/cne.24090

    Figure Lengend Snippet: Preabsorption controls for POMC, β-endorphin, ACTH and α-MSH antisera. (A1, A2) Adjacent sections from a brain with high POMC levels in tanycytes demonstrates that preincubation of the POMC antiserum with the immunizing peptide abolishes all POMC immunolabeling in tanycytes and neurons. (B1-B6) Preabsorption control for the β-endorphin antiserum in adjacent sections from a brain with intermediate POMC levels in tanycytes. Specific labeling is present in α2 tanycyte cell bodies and processes (arrow, B2), and in β2 and γ tanycytes (B3). In the section reacted with the preabsorbed antiserum (B4-6), some background labeling remains in the location of α2 tanycyte cell bodies, but this signal is indistinct. (C1, C2) Preabsorption control for the ACTH antiserum; inset shows an ACTH-positive γ tanycyte. (D1, D2) After preincubation of the α-MSH antiserum with excess α-MSH, a neurofilament-like nonspecific labeling remains in the internal median eminence (arrowheads). 3v, third ventricle; ME, median eminence. Scale bar: 100μm; 50μm on B2 (for B2, B3, B5, B6, inset of C1).

    Article Snippet: For dual immunofluorescence, sections were incubated in the cocktail of rabbit anti-β-endorphin (Phoenix Pharmaceuticals, Cat# H-022-33, RRID:AB_2314007) and sheep anti-α-MSH antisera (Millipore, Cat# AB5087, RRID:AB_91683; gift of Jeffrey B. Tatro) overnight followed by Cy3-conjugated donkey anti-rabbit (Jackson ImmunoResearch Labs, Cat# 711-165-152, RRID:AB_2307443) and Alexa 488-conjugated donkey anti-sheep IgG (Jackson ImmunoResearch Labs, Cat# 713-545-147, RRID:AB_2340745) for 2h.

    Techniques: Immunolabeling, Labeling