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Phenomenex chromatography chromatographic separations
Chromatography Chromatographic Separations, supplied by Phenomenex, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromatography chromatographic separations - by Bioz Stars, 2020-07
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Article Title: Determination of ciprofloxacin in human plasma using high-performance liquid chromatography coupled with fluorescence detection: Application to a population pharmacokinetics study in children with severe malnutrition
Article Snippet: .. 2.3 Chromatography Chromatographic separations were performed on a Synergi ® Max-RP analytical column (150 mm × 4.6 mm i.d., 4 μm particle size; Phenomenex Inc., Macclesfield, Cheshire, UK), protected by a guard column (LiChroSpher ® 100 RP-18e, 10 mm × 4.6 mm i.d., 5 μm particle size; Merck, Darmstadt, Germany), maintained at 40 °C, using a thermostatically controlled column heater. ..

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  • 91
    Phenomenex libertellenone m separation
    Structures of pimarane diterpenoids; myrocin F ( 1 ), <t>libertellenone</t> M ( 2 ), the suggested opened γ-lactone of libertellenone M ( 3 ), libertellenone C ( 4 ), and libertellenone E ( 5 ).
    Libertellenone M Separation, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    libertellenone m separation - by Bioz Stars, 2020-07
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    94
    Phenomenex hplc separation
    In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from <t>ETA</t> by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase <t>HPLC</t> retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.
    Hplc Separation, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Phenomenex helvolic acid separation
    Base peak chromatograms (BPC) of the EtOAc crude extract and three bioactive fractions (ranging from 40% to 100% organic) in positive electrospray ionization (ESI) mode. The fractions were obtained by RP flash chromatography with a gradient of MeCN and water going from 15% to 100% MeCN. In the bioactive fractions the marked peaks indicate the tentatively identified peptaiboitics, <t>helvolic</t> acid, ilicicolin H, and a potential new ilicicolin H analogue.
    Helvolic Acid Separation, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Structures of pimarane diterpenoids; myrocin F ( 1 ), libertellenone M ( 2 ), the suggested opened γ-lactone of libertellenone M ( 3 ), libertellenone C ( 4 ), and libertellenone E ( 5 ).

    Journal: Marine Drugs

    Article Title: A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria

    doi: 10.3390/md15080253

    Figure Lengend Snippet: Structures of pimarane diterpenoids; myrocin F ( 1 ), libertellenone M ( 2 ), the suggested opened γ-lactone of libertellenone M ( 3 ), libertellenone C ( 4 ), and libertellenone E ( 5 ).

    Article Snippet: Libertellenone M and the opened γ-lactone of libertellenone M separation was achieved on a Gemini C6 -Phenyl, 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min using a linear gradient 40% MeCN in Milli-Q water with 20 mM FA going to 100% MeCN in 28 min. Further libertellenone M separation was done on a Luna II C18 , 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min isocratic 55% MeCN in Milli-Q water with 20 mM FA in 20 min and a Kinetex Biphenyl, 5 μm 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min using a linear gradient 30% MeCN in Milli-Q water with 20 mM FA going to 100% MeCN in 25 min. Libertellenone C separation was achieved on a Luna II C18 , 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 5 mL/min using a linear gradient of 30% MeCN in Milli-Q water with 20 mM FA going to 70% MeCN in 30 min. Libertellenone E was purified from the EtOAc 3:1 MeOH fraction on the Waters 600 semipreparative HPLC.

    Techniques:

    Selected key NOESY correlations for libertellenone M ( 2 ).

    Journal: Marine Drugs

    Article Title: A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria

    doi: 10.3390/md15080253

    Figure Lengend Snippet: Selected key NOESY correlations for libertellenone M ( 2 ).

    Article Snippet: Libertellenone M and the opened γ-lactone of libertellenone M separation was achieved on a Gemini C6 -Phenyl, 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min using a linear gradient 40% MeCN in Milli-Q water with 20 mM FA going to 100% MeCN in 28 min. Further libertellenone M separation was done on a Luna II C18 , 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min isocratic 55% MeCN in Milli-Q water with 20 mM FA in 20 min and a Kinetex Biphenyl, 5 μm 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min using a linear gradient 30% MeCN in Milli-Q water with 20 mM FA going to 100% MeCN in 25 min. Libertellenone C separation was achieved on a Luna II C18 , 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 5 mL/min using a linear gradient of 30% MeCN in Milli-Q water with 20 mM FA going to 70% MeCN in 30 min. Libertellenone E was purified from the EtOAc 3:1 MeOH fraction on the Waters 600 semipreparative HPLC.

    Techniques:

    In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from ETA by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase HPLC retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Ethionamide activation and sensitivity in multidrug-resistant Mycobacterium tuberculosis

    doi:

    Figure Lengend Snippet: In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from ETA by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase HPLC retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.

    Article Snippet: HPLC separation of the [14 C]ETA metabolite mixture was achieved by using a reverse-phase LUNA column [5 μm, C18(2), 250 × 4.6 mm, Phenomenex, Torrence, CA] with a gradient of (0–5 min) 0% acetonitrile, 100% water; then (5–65 min) to 70% acetonitrile; then (65–80 min) to 100% acetonitrile (all solvents contained 0.1% trifluoroacetic acid).

    Techniques: In Vivo, Incubation, Thin Layer Chromatography, Autoradiography, Filtration, Radioactivity, High Performance Liquid Chromatography, Isolation

    Base peak chromatograms (BPC) of the EtOAc crude extract and three bioactive fractions (ranging from 40% to 100% organic) in positive electrospray ionization (ESI) mode. The fractions were obtained by RP flash chromatography with a gradient of MeCN and water going from 15% to 100% MeCN. In the bioactive fractions the marked peaks indicate the tentatively identified peptaiboitics, helvolic acid, ilicicolin H, and a potential new ilicicolin H analogue.

    Journal: Marine Drugs

    Article Title: A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria

    doi: 10.3390/md15080253

    Figure Lengend Snippet: Base peak chromatograms (BPC) of the EtOAc crude extract and three bioactive fractions (ranging from 40% to 100% organic) in positive electrospray ionization (ESI) mode. The fractions were obtained by RP flash chromatography with a gradient of MeCN and water going from 15% to 100% MeCN. In the bioactive fractions the marked peaks indicate the tentatively identified peptaiboitics, helvolic acid, ilicicolin H, and a potential new ilicicolin H analogue.

    Article Snippet: Myrocin F separation was achieved on a Luna II C18 , 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 5 mL/min using a linear gradient of 45% MeCN in Milli-Q water with 20 mM FA going to 75% MeCN in 20 min. Helvolic acid separation was achieved on a Luna II C18 , 5 μm, 250 × 10 mm column (Phenomenex, Torrance, CA, USA) with a flow rate of 4 mL/min using a linear gradient 60% MeCN in Milli-Q water going to 100% MeCN in 20 min. Cultivation 2: Extraction was achieved using 600 mL per flask of EtOAc with 1% FA.

    Techniques: Chromatography