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Agilent technologies chromatography chromatographic separation
Chromatography Chromatographic Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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High Performance Liquid Chromatography:

Article Title: 1H NMR-Based Metabolomics Coupled With Molecular Docking Reveal the Anti-Diabetic Effects and Potential Active Components of Berberis vernae on Type 2 Diabetic Rats
Article Snippet: .. HPLC Analysis of B. vernae Extract Chromatographic separation was performed on an Agilent 1260 Series HPLC system equipped with a quaternary pump, temperature-controlled autosampler, and diode array detector recorded from 200 to 400 nm (Agilent technologies, Germany). .. A WondaSil C18 column (250 mm × 4.6 mm, 5 μm, Shimadzu, Japan) was used.

Article Title: Ultrasound-Assisted Extraction of Arabinogalactan and Dihydroquercetin Simultaneously from Larix gmelinii as a Pretreatment for Pulping and Papermaking
Article Snippet: The HPLC-UV chromatograms of the DHQ standard and a larch wood sample are shown in and 1(c). .. LC-ESI-MS qualitative analysis method of DHQ The HPLC-ESI-MS system consisted of an Agilent 1100 series HPLC system equipped with a G1312A Bin pump, a G1379A Degasser (Agilent, San Jose, CA, USA) and a G1316A automatic column temperature control box. ..

Article Title: Aldo-keto Reductase Metabolizes Glyphosate and Confers Glyphosate Resistance in Echinochloa colona 1
Article Snippet: .. HPLC-Q-TOF-MS Analysis of Glyphosate Metabolites by E . coli -Expressed EcAKR4-1 Enzyme Chromatographic separations of glyphosate and its possible metabolites (AMPA, glyoxylate, sarcosine, and formaldehyde) were achieved with the 1290 HPLC system (Agilent Technologies) on a XAqua C18 column (2.1 mm × 150 mm, particle size 5 μm; Acchrom). .. The mobile phase consisted of 0.1% (v/v) formic acid (FA) aqueous solution (solvent A) and acetonitrile (ACN; solvent B) with a flow rate of 0.3 mL min−1 and an injection volume of 5 μL.

Article Title: Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain
Article Snippet: .. The surfactin concentration was determined by high performance liquid chromatography (HPLC) using an Agilent Technologies model 1220 device equipped with a diode detector. .. The chromatographic separation was carried out under the following conditions: Poroshell 120 EC-C18 column (4.6 × 150 mm), mobile phase 80:20 (acetonitrile: 3.8 mM trifluoroacetic acid), 40 °C, detection at 205 nm.

Article Title: Identification of Bioactive Compounds of Asparagus officinalis L.: Permutation Test Allows Differentiation among “Triguero” and Hybrid Green Varieties
Article Snippet: Analytical Characterization The asparagus extracts were analytically characterized by high-performance liquid chromatography coupled to electro-spray time-of-flight mass spectrometry (HPLC-DAD-ESI-TOF/MS). .. The HPLC-DAD-ESI-TOF/MS method was performed in an Agilent 1200-HPLC system (Agilent Technologies, Waldbronn, Germany) of the Series Rapid Resolution equipped with a vacuum degasser, autosampler, a binary pump, and a diode-array detector (DAD). .. The chromatographic separation was performed in a Zorbax Eclipse Plus RP-C18 analytical column (Agilent Technologies, Palo Alto, CA, USA) 150 × 4.6 mm i.d., 1.8 μm particle size).

Chromatography:

Article Title: Naoling decoction restores cognitive function by inhibiting the neuroinflammatory network in a rat model of Alzheimer’s disease
Article Snippet: .. Liquid chromatography–mass spectrometry quadrapole time of flight (LC/MS-Q-TOF) analysis of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (THSG) and Icariin determination The Agilent LC/MS-Q-TOF system (Santa Clara, CA, USA) was setup in the negative electrospray ionization (ESI) mode for chromatographic separation of THSG amd Icariin in an Agilent Technologies 1290 liquid chromatograph with an Cosmosil MS-II C18 column (250 × 4.6 mm, 5 μm). ..

Mass Spectrometry:

Article Title: Naoling decoction restores cognitive function by inhibiting the neuroinflammatory network in a rat model of Alzheimer’s disease
Article Snippet: .. Liquid chromatography–mass spectrometry quadrapole time of flight (LC/MS-Q-TOF) analysis of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside (THSG) and Icariin determination The Agilent LC/MS-Q-TOF system (Santa Clara, CA, USA) was setup in the negative electrospray ionization (ESI) mode for chromatographic separation of THSG amd Icariin in an Agilent Technologies 1290 liquid chromatograph with an Cosmosil MS-II C18 column (250 × 4.6 mm, 5 μm). ..

Concentration Assay:

Article Title: Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain
Article Snippet: .. The surfactin concentration was determined by high performance liquid chromatography (HPLC) using an Agilent Technologies model 1220 device equipped with a diode detector. .. The chromatographic separation was carried out under the following conditions: Poroshell 120 EC-C18 column (4.6 × 150 mm), mobile phase 80:20 (acetonitrile: 3.8 mM trifluoroacetic acid), 40 °C, detection at 205 nm.

Activity Assay:

Article Title: PDE7B is involved in nandrolone decanoate hydrolysis in liver cytosol and its transcription is up-regulated by androgens in HepG2
Article Snippet: After a selected incubation time, the reaction mixture was stopped by adding 100 μL acetonitril from Merck and centrifuged 10 min at 3500 rpm prior to injection of 20 μL onto a high performance liquid chromatography system coupled to ultra-violet detection (HPLC-UV). .. Esterase activity was determined by monitoring the nandrolone formation by analysis on an Agilent 1100 LC system from Agilent Technologies (Palo Alto, CA, USA) system coupled to a UV detector Agilent 1200 sets at a wavelength of 242 nm. .. The chromatographic separation was performed on a C18 Luna (100 × 4.6, 3 μm) column from Phenomenex Inc. (Torrance, CA, USA) with an isocratic flow of acetonitril/H2O (40:60, v:v) at 1.0 mL/min.

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  • 99
    Agilent technologies nano hplc
    HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography <t>(RP-HPLC)</t> to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by <t>nano-HPLC</t> coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.
    Nano Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Agilent technologies zorbax eclipse xdb c18 column
    Representative LC-ESI MS/MS MRM (multi reaction monitoring) chromatogram of urophysial sample of round goby. Chromatographic conditions: Agilent <t>Zorbax</t> Extend Plus <t>C18</t> column (50 mm × 2.1 mm I.D., 1.8 μm particle); elution: solvent A (0.1 % acetic acid in H 2 O), solvent B [0.1 % acetic acid in acetonitrile: H 2 O (3:1)], a gradient elution was used starting from 5 to 30 % B in 5.3 min; flow rate 0.6 mL/min; column temperature 20 °C; injection volume 5 μL; the monitored mass transitions for AVT were set at m/z 525.5 → 517.2 and for IT were set at m/z 483.7 → 136.1
    Zorbax Eclipse Xdb C18 Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zorbax eclipse xdb c18 column/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
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    zorbax eclipse xdb c18 column - by Bioz Stars, 2021-07
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    99
    Agilent technologies ultra performance liquid chromatography uplc accurate mass quadrupole time of flight
    <t>Q-TOF</t> <t>UPLC/MS</t> spectra in negative ion mode and chemical structures of ginsenoside Re. MS scan data for ginsenoside Re (a). MS 2 patterns were obtained with indicated collision energies (CE) 40 eV (b) and CE 50 eV (c).
    Ultra Performance Liquid Chromatography Uplc Accurate Mass Quadrupole Time Of Flight, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra performance liquid chromatography uplc accurate mass quadrupole time of flight/product/Agilent technologies
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    HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by nano-HPLC coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.

    Journal: Mucosal Immunology

    Article Title: Paneth cell α-defensin 6 (HD-6) is an antimicrobial peptide

    doi: 10.1038/mi.2014.100

    Figure Lengend Snippet: HD-6 is reduced in a one-step reduction without intermediate, partly reduced forms. ( a ) oxHD-6 was incubated with or without 2 m M DTT, alkylated with iodoacetamide, and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Mass spectra contain mass-to-charge ratios of singly protonated ions: 3,706.62 m/z for oxidized HD-6 and 4,054.95 m/z for completely reduced and sixfold alkylated HD-6. Each alkylated, formerly free cysteine results in an increased m/z of +57. ( b ) oxHD-6 or ( c ) ΔoxHD-6 was incubated with or without 2 m M DTT and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) to investigate hydrophobicity and intermediate forms of HD-6. ( d ) oxHD-6 was incubated with 2 m M DTT and analyzed by nano-HPLC coupled to a quadrupole-time-of-flight mass spectrometer equipped with an electrospray-ionization source. The mass spectra of the peaks contain mass-to-charge ratios ( m/z ) that correspond to 3-, 4-, 5-, and 6-fold protonated ions of either oxidized HD-6 (neutral mass M=3,705.4961 Da) or completely reduced HD-6 (neutral mass M=3,711.5430 Da). The neutral mass difference of ∼6 reflects the difference of six hydrogen atoms between the two redox forms. The signal at 1,221.9906 m/z is derived from the reference mass that is present in all analyses. For each subfigure, one representative experiment out of three independent experiments is shown. DTT, dithiothreitol; HD-6, α-defensin 6; IAA, iodoacetamide; oxHD-6, oxidized HD-6; redHD-6, reduced HD-6; UV, ultraviolet.

    Article Snippet: Chromatographic separation was carried out by nano-HPLC (ChipCube, Agilent) with a reversed-phase column using an Agilent HPLC ProtID-Chip-43 with a 40 nl enrichment column and a 75 μl × 43 mm analytical column made of ZORBAX 300SB-C18 5 μm material.

    Techniques: Incubation, Mass Spectrometry, High Performance Liquid Chromatography, Derivative Assay

    Representative LC-ESI MS/MS MRM (multi reaction monitoring) chromatogram of urophysial sample of round goby. Chromatographic conditions: Agilent Zorbax Extend Plus C18 column (50 mm × 2.1 mm I.D., 1.8 μm particle); elution: solvent A (0.1 % acetic acid in H 2 O), solvent B [0.1 % acetic acid in acetonitrile: H 2 O (3:1)], a gradient elution was used starting from 5 to 30 % B in 5.3 min; flow rate 0.6 mL/min; column temperature 20 °C; injection volume 5 μL; the monitored mass transitions for AVT were set at m/z 525.5 → 517.2 and for IT were set at m/z 483.7 → 136.1

    Journal: Fish Physiology and Biochemistry

    Article Title: Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species

    doi: 10.1007/s10695-012-9746-6

    Figure Lengend Snippet: Representative LC-ESI MS/MS MRM (multi reaction monitoring) chromatogram of urophysial sample of round goby. Chromatographic conditions: Agilent Zorbax Extend Plus C18 column (50 mm × 2.1 mm I.D., 1.8 μm particle); elution: solvent A (0.1 % acetic acid in H 2 O), solvent B [0.1 % acetic acid in acetonitrile: H 2 O (3:1)], a gradient elution was used starting from 5 to 30 % B in 5.3 min; flow rate 0.6 mL/min; column temperature 20 °C; injection volume 5 μL; the monitored mass transitions for AVT were set at m/z 525.5 → 517.2 and for IT were set at m/z 483.7 → 136.1

    Article Snippet: Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle size).

    Techniques: Mass Spectrometry, Flow Cytometry, Injection

    Representative HPLC-FL chromatograms of a urophysial sample of round goby and b the same sample spiked with standard AVT (6.6 pmol/mL) and IT (6.8 pmol/mL). Chromatographic conditions: Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle); elution: solvent A (0.1 % TFA in H 2 O), solvent B [0.1 % TFA in acetonitrile : H 2 O (3:1)], linear gradient 45–80 % of eluent B in 12 min; flow rate 1 mL/min; column temperature 20 °C; injection volume 40 μL; detection: FL, excitation 470 nm, emission 530 nm

    Journal: Fish Physiology and Biochemistry

    Article Title: Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species

    doi: 10.1007/s10695-012-9746-6

    Figure Lengend Snippet: Representative HPLC-FL chromatograms of a urophysial sample of round goby and b the same sample spiked with standard AVT (6.6 pmol/mL) and IT (6.8 pmol/mL). Chromatographic conditions: Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle); elution: solvent A (0.1 % TFA in H 2 O), solvent B [0.1 % TFA in acetonitrile : H 2 O (3:1)], linear gradient 45–80 % of eluent B in 12 min; flow rate 1 mL/min; column temperature 20 °C; injection volume 40 μL; detection: FL, excitation 470 nm, emission 530 nm

    Article Snippet: Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 column (150 mm × 4.6 mm I.D., 5 μm particle size).

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection

    Purification of MHC class II-associated peptides. A. RP-HPLC chromatogram of elution of the MHCII associated peptides. System: Agilent 1100, Column: Agilent ZORBAX 300SB-C18, Flow rate : 1 mL/min and a gradient was created by mixing Solvent A: 0.1% TFA (v/v) in CH3COOH(8.7%) and HCOOH(2.2%), pH1.9, and Solvent B: 0.08% TFA (v/v) in Acetonitrile; B. Dot blot of RP-HPLC fractions. 5 µL from each fraction was applied to a blotting membrane and I-A b molecules were detected as described in the text. C. Quantitative Imaging Analysis of MHCII positive fractions from GILT-WT and GILT−/− samples. MHC class II positive fractions from RP-HPLC were pooled for each sample and three different amounts (high: 25 µL, medium: 12.5 µL, and low: 6µL) from each pool were loaded onto SDS-PAGE gel for quantitative imaging. D. Flow cytometry analysis of GILT−/− and GILT-WT splenocytes. Spleens were isolated, ground and red blood cells lysed in hypertonic buffer. Washed and filtered splenocytes were incubated 30 min. on ice with anti-IA b M5/114–PE antibody.

    Journal: PLoS ONE

    Article Title: Comparative Quantitative Mass Spectrometry Analysis of MHC Class II-Associated Peptides Reveals a Role of GILT in Formation of Self-Peptide Repertoire

    doi: 10.1371/journal.pone.0010599

    Figure Lengend Snippet: Purification of MHC class II-associated peptides. A. RP-HPLC chromatogram of elution of the MHCII associated peptides. System: Agilent 1100, Column: Agilent ZORBAX 300SB-C18, Flow rate : 1 mL/min and a gradient was created by mixing Solvent A: 0.1% TFA (v/v) in CH3COOH(8.7%) and HCOOH(2.2%), pH1.9, and Solvent B: 0.08% TFA (v/v) in Acetonitrile; B. Dot blot of RP-HPLC fractions. 5 µL from each fraction was applied to a blotting membrane and I-A b molecules were detected as described in the text. C. Quantitative Imaging Analysis of MHCII positive fractions from GILT-WT and GILT−/− samples. MHC class II positive fractions from RP-HPLC were pooled for each sample and three different amounts (high: 25 µL, medium: 12.5 µL, and low: 6µL) from each pool were loaded onto SDS-PAGE gel for quantitative imaging. D. Flow cytometry analysis of GILT−/− and GILT-WT splenocytes. Spleens were isolated, ground and red blood cells lysed in hypertonic buffer. Washed and filtered splenocytes were incubated 30 min. on ice with anti-IA b M5/114–PE antibody.

    Article Snippet: Chromatographic separation of peptides and I-Ab molecules was achieved on Agilent Zorbax 300SB-C18 column (4.6 mm ID×150 mm, 5 µm), with an Agilent Zorbax High Pressure, Reliance Cartridge, (4.6 mm ID×12.5 mm) Guard-Column.

    Techniques: Purification, High Performance Liquid Chromatography, Flow Cytometry, Dot Blot, Imaging, SDS Page, Cytometry, Isolation, Incubation, IA

    Q-TOF UPLC/MS spectra in negative ion mode and chemical structures of ginsenoside Re. MS scan data for ginsenoside Re (a). MS 2 patterns were obtained with indicated collision energies (CE) 40 eV (b) and CE 50 eV (c).

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effect of Ginseng (Panax ginseng) Berry EtOAc Fraction on Cognitive Impairment in C57BL/6 Mice under High-Fat Diet Inducement

    doi: 10.1155/2015/316527

    Figure Lengend Snippet: Q-TOF UPLC/MS spectra in negative ion mode and chemical structures of ginsenoside Re. MS scan data for ginsenoside Re (a). MS 2 patterns were obtained with indicated collision energies (CE) 40 eV (b) and CE 50 eV (c).

    Article Snippet: Ultra-Performance Liquid Chromatography (UPLC) Accurate-Mass Quadrupole Time-of-Flight (Q-TOF)/MS Analysis The chromatographic separation was performed using an ultra-performance liquid chromatography (UPLC) accurate-mass quadrupole time-of-flight (Q-TOF) MS (Agilent Technologies, Santa Clara, CA, USA), and column was used to a ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μ m particle size; Waters Corp, Milford, MA, USA) with a flow rate of 0.3 mL/min and oven temperature at 40°C.

    Techniques: Mass Spectrometry