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Agilent technologies chromatography chromatographic separation
Chromatography Chromatographic Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromatography chromatographic separation - by Bioz Stars, 2020-07
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Article Title: Urine and serum metabolomic profiling reveals that bile acids and carnitine may be potential biomarkers of primary biliary cirrhosis
Article Snippet: .. Chromatography Chromatographic separation was performed on a 15 cmx2.1 mm Acquity™ 1.8 µ m C18 column (Agilent Technologies, Santa Clara, CA, USA) using an Acquity™ ultra performance liquid chromatography system (Ultimate 3000-Bruker mXis; Dionex, Sunnyvale, CA, USA). .. A 10-µ l aliquot of each sample was injected into the column.

Chromatography:

Article Title: Urine and serum metabolomic profiling reveals that bile acids and carnitine may be potential biomarkers of primary biliary cirrhosis
Article Snippet: .. Chromatography Chromatographic separation was performed on a 15 cmx2.1 mm Acquity™ 1.8 µ m C18 column (Agilent Technologies, Santa Clara, CA, USA) using an Acquity™ ultra performance liquid chromatography system (Ultimate 3000-Bruker mXis; Dionex, Sunnyvale, CA, USA). .. A 10-µ l aliquot of each sample was injected into the column.

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    Agilent technologies hplc q tof ms conditions chromatographic separation
    The mass spectra of the branched-chain keto acids standards using <t>HPLC-Q-TOF/MS.</t> ( A ) Full scan mass spectra of α-keto-β-methylvalerate (KMV); ( B ) Full scan mass spectrum of α-ketoisovalerate (KIV); ( C ) Full scan mass spectrum of α-ketoisovalerate (KIC); ( D ) MS/MS spectrum of KMV; ( E ) MS/MS spectrum of KIV; ( F ) MS/MS spectrum of KIC.
    Hplc Q Tof Ms Conditions Chromatographic Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc q tof ms conditions chromatographic separation/product/Agilent technologies
    Average 92 stars, based on 2 article reviews
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    92
    Agilent technologies preparative hsccc separation
    (a) <t>HPLC</t> chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with <t>HSCCC.</t> Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.
    Preparative Hsccc Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies hplc esi tof ms chromatographic separation
    <t>HPLC-ESI-TOF-MS</t> total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group B.
    Hplc Esi Tof Ms Chromatographic Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies assay conditions hplc separation
    <t>HPLC</t> chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the <t>Agilent</t> 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).
    Assay Conditions Hplc Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The mass spectra of the branched-chain keto acids standards using HPLC-Q-TOF/MS. ( A ) Full scan mass spectra of α-keto-β-methylvalerate (KMV); ( B ) Full scan mass spectrum of α-ketoisovalerate (KIV); ( C ) Full scan mass spectrum of α-ketoisovalerate (KIC); ( D ) MS/MS spectrum of KMV; ( E ) MS/MS spectrum of KIV; ( F ) MS/MS spectrum of KIC.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Determination of Branched-Chain Keto Acids in Serum and Muscles Using High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

    doi: 10.3390/molecules23010147

    Figure Lengend Snippet: The mass spectra of the branched-chain keto acids standards using HPLC-Q-TOF/MS. ( A ) Full scan mass spectra of α-keto-β-methylvalerate (KMV); ( B ) Full scan mass spectrum of α-ketoisovalerate (KIV); ( C ) Full scan mass spectrum of α-ketoisovalerate (KIC); ( D ) MS/MS spectrum of KMV; ( E ) MS/MS spectrum of KIV; ( F ) MS/MS spectrum of KIC.

    Article Snippet: HPLC Q-TOF/MS Conditions Chromatographic separation of three BCKAs was achieved on an Agilent Eclipse Plus C18 column (2.1 × 100 mm, 1.8 μm).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

    doi: 10.1155/2018/3158293

    Figure Lengend Snippet: (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Article Snippet: The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA).

    Techniques: High Performance Liquid Chromatography, Purification, High Speed Counter-current Chromatography, Flow Cytometry, Injection

    HPLC-ESI-TOF-MS total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group B.

    Journal: PLoS ONE

    Article Title: Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave

    doi: 10.1371/journal.pone.0200174

    Figure Lengend Snippet: HPLC-ESI-TOF-MS total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group B.

    Article Snippet: Flavonoid analysis of D . erythrosora leaves with different drying pretreatments using HPLC-ESI-TOF-MS Chromatographic separation was performed on an Agilent 1100 HPLC system (USA Agilent Technologies), equipped with a binary pump, a microdegasser, Hi-performance well-plate auto sampler, thermostat column compartment, and diode-array detector (DAD).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    HPLC-ESI-TOF-MS total ion chromatograms of ethyl acetate and n-butanol of extracts from group A.

    Journal: PLoS ONE

    Article Title: Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave

    doi: 10.1371/journal.pone.0200174

    Figure Lengend Snippet: HPLC-ESI-TOF-MS total ion chromatograms of ethyl acetate and n-butanol of extracts from group A.

    Article Snippet: Flavonoid analysis of D . erythrosora leaves with different drying pretreatments using HPLC-ESI-TOF-MS Chromatographic separation was performed on an Agilent 1100 HPLC system (USA Agilent Technologies), equipped with a binary pump, a microdegasser, Hi-performance well-plate auto sampler, thermostat column compartment, and diode-array detector (DAD).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    HPLC-ESI-TOF-MS total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group C.

    Journal: PLoS ONE

    Article Title: Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave

    doi: 10.1371/journal.pone.0200174

    Figure Lengend Snippet: HPLC-ESI-TOF-MS total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group C.

    Article Snippet: Flavonoid analysis of D . erythrosora leaves with different drying pretreatments using HPLC-ESI-TOF-MS Chromatographic separation was performed on an Agilent 1100 HPLC system (USA Agilent Technologies), equipped with a binary pump, a microdegasser, Hi-performance well-plate auto sampler, thermostat column compartment, and diode-array detector (DAD).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    HPLC-ESI-TOF-MS total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group D.

    Journal: PLoS ONE

    Article Title: Effects of different pretreatments on flavonoids and antioxidant activity of Dryopteris erythrosora leave

    doi: 10.1371/journal.pone.0200174

    Figure Lengend Snippet: HPLC-ESI-TOF-MS total ion chromatograms of dichloromethane, ethyl acetate, and n-butanol of extracts from group D.

    Article Snippet: Flavonoid analysis of D . erythrosora leaves with different drying pretreatments using HPLC-ESI-TOF-MS Chromatographic separation was performed on an Agilent 1100 HPLC system (USA Agilent Technologies), equipped with a binary pump, a microdegasser, Hi-performance well-plate auto sampler, thermostat column compartment, and diode-array detector (DAD).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    HPLC chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).

    Journal: Molecular Vision

    Article Title: Pharmacokinetic study of mangiferin in rat plasma and retina using high-performance liquid chromatography

    doi:

    Figure Lengend Snippet: HPLC chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).

    Article Snippet: Assay conditions HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Injection

    HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A : Typical chromatogram of blank eye sample. B : Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C : Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).

    Journal: Molecular Vision

    Article Title: Pharmacokinetic study of mangiferin in rat plasma and retina using high-performance liquid chromatography

    doi:

    Figure Lengend Snippet: HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A : Typical chromatogram of blank eye sample. B : Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C : Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).

    Article Snippet: Assay conditions HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Injection